LOCUS       sequence-296452-        3597 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector, with a fragmented insert of S 
            gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3597)
  AUTHORS   Kim DK, Knapp JJ, Kuang D, Chawla A, Cassonnet P, Lee H, 
            Sheykhkarimli D, Samavarchi-Tehrani P, Abdouni H, Rayhan A, Li R, 
            Pogoutse O, Coyaud E, van der Werf S, Demeret C, Gingras AC, Taipale
            M, Raught B, Jacob Y, Roth FP
  TITLE     A Comprehensive, Flexible Collection of SARS-CoV-2 Coding Regions.
  JOURNAL   G3 (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554.
  PUBMED    32763951
REFERENCE   2  (bases 1 to 3597)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554."
COMMENT     Sequence Label: pDONR221 SARS-CoV-2 S-frag2_nostop
FEATURES             Location/Qualifiers
     source          1..3597
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atggtgaacc tgaccaccag aacccagctg ccacctgcct acaccaactc cttcaccagg
       61 ggcgtgtact acccagacaa ggtgttcaga tccagcgtgc tgcactctac ccaggacctg
      121 ttcctgccat tcttctctaa cgtgacctgg ttccacgcca tccacgtgtc tggaaccaac
      181 ggcaccaaga gattcgacaa cccagtgctg ccattcaacg acggcgtgta cttcgcttct
      241 accgagaagt ctaacatcat caggggttgg atcttcggaa ccaccctgga ctctaagacc
      301 cagtctctgc tgatcgtgaa caacgctacc aacgtggtga tcaaggtgtg cgagttccag
      361 ttctgcaacg acccattcct gggagtgtac taccacaaga acaacaagtc ttggatggaa
      421 agcgagttca gggtgtacag ctctgctaac aactgcacct tcgagtacgt gtctcagcct
      481 ttcctgatgg acctggaagg aaagcaggga aacttcaaga acctgagaga gttcgtgttc
      541 aagaacatcg acggttactt caagatatac agcaagcaca ccccaatcaa cctggtgagg
      601 gacctgccac agggtttctc tgctctggag cccctggtgg acctgccaat cggcatcaac
      661 atcaccaggt tccagaccct gctggctctg cacaggtctt acctgacccc aggcgacagc
      721 agctctggat ggaccgccgg cgccgctgct tactacgtgg gatacctgca gccaaggacc
      781 ttcctgctga agtacaacga aaacggcacc atcaccgacg ccgtggactg cgctctggac
      841 ccactgtctg agaccaagtg caccctgaag tctttcaccg tggaaaaggg aatctaccag
      901 accagcaact tccgcgtgca gcccaccgag tctatcgtga gattcccaaa catcaccaac
      961 ctgtgcccat tcggagaagt gttcaacgct accaggttcg ccagcgtgta cgcttggaac
     1021 aggaagagaa tcagcaactg cgtggccgac tactctgtgc tgtacaactc tgcctccttc
     1081 tccaccttca agtgctacgg cgtgtcccca accaagctga acgacctgtg cttcaccaac
     1141 gtgtacgctg actccttcgt gatcagagga gacgaagtga gacagatcgc tcccggtcag
     1201 accggaaaga tcgccgacta caactacaag ctgccagacg acttcaccgg ttgcgtgatc
     1261 gcttggaact ctaacaacct ggactctaag gtgggaggca actacaacta cctgtacagg
     1321 ctgttccgca agagcaacct gaagcctttc gaaagggaca tcagcaccga aatctaccag
     1381 gctggatcta ccccatgcaa cggagtggaa ggattcaact gctacttccc tctgcagtct
     1441 tacggattcc agccaaccaa cggagtggga taccagccat acagagtggt ggtgctgtct
     1501 ttcgagctgc tgcacgctcc tgctaccgtg tgcggaccaa agaagtctac caacctggtg
     1561 aagaacaagt gcgtgaactt caacttcaac ggactgaccg gaaccggagt gctgaccgaa
     1621 tccaacaaga agttcctgcc tttccagcag ttcggtagag acatcgccga caccaccgac
     1681 gctgtgagag acccacagac cctggaaatc ctggacatca ccccctgctc tttcggagga
     1741 gtgagcgtga tcaccccagg aaccaacacc tccaaccagg tggccgtgct gtaccaggac
     1801 gtgaactgca ccgaagtgcc agtggccatc cacgctgacc agctgacccc aacctggaga
     1861 gtgtacagca ccggatctaa cgtgttccag acccgtgccg gctgcctgat cggagccgag
     1921 cacgtgaaca actcctacga atgcgacatc ccaatcggag ctggcatctg cgcttcttac
     1981 cagacccaga ccaacagccc aagaagggcc agatccgtgg cctctcagag catcatcgcc
     2041 tacaccatga gcctgggcgc tgaaaacagc gtggcttaca gcaacaactc tatcgccatc
     2101 ccaaccaact tcaccatctc tgtgaccacc gaaatcctgc cagtgagcat gaccaagacc
     2161 agcgtggact gcaccatgta catctgcgga gactctaccg aatgctctaa cctgctgctg
     2221 cagtacggca gcttctgcac ccagctgaac cgcgctctga ccggaatcgc tgtggagcag
     2281 gacaagaaca cccaggaagt gttcgctcag gtgaagcaga tatacaagac cccacctatc
     2341 aaggacttcg gaggattcaa cttctctcag atcctgcccg acccatctaa gccctctaag
     2401 aggtctttca tcgaagacct gctgttcaac aaggtgaccc tggccgacgc cggtttcatc
     2461 aagcagtacg gtgactgcct gggagacatc gccgctagag acctgatctg cgcccagaag
     2521 ttcaacggtc tgaccgtgct gccaccactg ctgaccgacg agatgatcgc ccagtacacc
     2581 tctgccctgc tggctggaac catcacctct ggttggacct tcggagctgg cgccgccctg
     2641 cagatcccat tcgctatgca gatggcctac cgtttcaacg gtatcggagt gacccagaac
     2701 gtgctgtacg aaaaccagaa gctgatcgct aaccagttca actctgctat cggaaagatc
     2761 caggactccc tgtcctctac cgcttctgcc ctgggaaagc tgcaggacgt ggtgaaccag
     2821 aacgcccagg ccctgaacac cctggtgaag cagctgtctt ctaacttcgg agccatctcc
     2881 tctgtgctga acgacatcct gtctaggctg gacaaggtgg aagccgaggt gcagatcgac
     2941 agactgatca ccggaagact gcagtctctg cagacctacg tgacccagca gctgatcagg
     3001 gccgctgaaa tcagagcttc tgccaacctg gctgccacca agatgtccga atgcgtgctg
     3061 ggccagtcta agagggtgga cttctgcgga aagggatacc acctgatgtc cttcccacag
     3121 tctgctccac acggagtggt gttcctgcac gtgacctacg tgcccgccca ggaaaagaac
     3181 ttcaccaccg ccccagctat ctgccacgac ggaaaggctc acttccccag ggagggagtg
     3241 ttcgtgtcta acggaaccca ctggttcgtg acccagagga acttctacga gccacagatc
     3301 atcaccaccg acaacacctt cgtgtctgga aactgcgacg tggtgatcgg aatcgtgaac
     3361 aacaccgtgt acgacccact gcagccagaa ctggactctt tcaaggagga actggacaag
     3421 tacttcaaga accacacctc tccagacgtg gacctgggag acatctccgg aatcaacgcc
     3481 tctgtggtga acatccagaa ggaaatcgac agactgaacg aggtggccaa gaacctgaac
     3541 gaatctctga tcgacctgca ggaactggga aagtacgagc agtacatcaa gtggccc
//
