LOCUS       sequence-296463-        4596 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector, with insert of NSP13 gene's CDS 
            from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4596)
  AUTHORS   Kim DK, Knapp JJ, Kuang D, Chawla A, Cassonnet P, Lee H, 
            Sheykhkarimli D, Samavarchi-Tehrani P, Abdouni H, Rayhan A, Li R, 
            Pogoutse O, Coyaud E, van der Werf S, Demeret C, Gingras AC, Taipale
            M, Raught B, Jacob Y, Roth FP
  TITLE     A Comprehensive, Flexible Collection of SARS-CoV-2 Coding Regions.
  JOURNAL   G3 (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554.
  PUBMED    32763951
REFERENCE   2  (bases 1 to 4596)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554."
COMMENT     Sequence Label: pDONR223 SARS-CoV-2 NSP13_nostop
FEATURES             Location/Qualifiers
     source          1..4596
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(129..156)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(248..334)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     383..405
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     397..414
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     398..414
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    430..529
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2336..2435)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2452..2471)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2453..2471)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2476..2492)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2476..2492)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2923..3714
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3807..4395
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4296..4315
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4549..4566
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
       61 aaagcgggca gtgagcgcaa cgcaattaat acgcgtaccg ctagccagga agagtttgta
      121 gaaacgcaaa aaggccatcc gtcaggatgg ccttctgctt agtttgatgc ctggcagttt
      181 atggcgggcg tcctgcccgc caccctccgg gccgttgctt cacaacgttc aaatccgctc
      241 ccggcggatt tgtcctactc aggagagcgt tcaccgacaa acaacagata aaacgaaagg
      301 cccagtcttc cgactgagcc tttcgtttta tttgatgcct ggcagttccc tactctcgcg
      361 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaag
      421 ctcgggcccc aaataatgat tttattttga ctgatagtga cctgttcgtt gcaacaaatt
      481 gatgagcaat gcttttttat aatgccaact ttgtacaaaa aagttggcat ggccgtgggc
      541 gcttgcgtgc tgtgcaactc tcagaccagc ctgagatgtg gagcttgcat ccgcaggcct
      601 ttcctgtgct gcaagtgctg ctacgaccac gtgatcagca cctcccacaa gctggtgctg
      661 tccgtgaacc catacgtgtg caacgctcct ggttgtgacg tgactgacgt gacccagctg
      721 tacctgggcg gaatgagcta ctactgcaag tcccacaagc cccctatcag cttcccactg
      781 tgcgctaacg gccaggtgtt cggactgtac aagaacacct gcgtgggctc cgacaacgtg
      841 accgacttca acgccatcgc tacctgcgac tggaccaacg ccggagacta catcctggct
      901 aacacctgca ccgaaaggct gaagctgttc gccgctgaga ccctgaaggc caccgaggaa
      961 accttcaagc tgtcttacgg tatcgctacc gtgcgtgagg tgctgtctga ccgcgaactg
     1021 cacctgtctt gggaagtggg caagccaaga ccacccctga accgtaacta cgtgttcacc
     1081 ggatacaggg tgaccaagaa cagcaaggtg cagatcggag agtacacctt cgaaaaggga
     1141 gactacggtg acgccgtggt gtacagagga accaccacct acaagctgaa cgtgggtgac
     1201 tacttcgtgc tgacctccca caccgtgatg ccactgtctg ctcccaccct ggtgcctcag
     1261 gaacactacg tgcgcatcac cggactgtac ccaaccctga acatctccga cgagttcagc
     1321 tccaacgtgg ccaactacca gaaagtgggc atgcagaagt actccaccct gcagggacct
     1381 ccaggtaccg gcaagtctca cttcgccatc ggtctggctc tgtactaccc ctctgcccgt
     1441 atcgtgtaca ctgcttgttc tcacgctgct gtggatgctc tgtgcgagaa ggctctgaag
     1501 tacctgccta tcgacaagtg ctcccgcatc atcccagcca gagctcgtgt ggaatgcttc
     1561 gacaagttca aggtgaactc taccctggag cagtacgtgt tctgcaccgt gaacgccctg
     1621 cctgaaacca ccgctgacat cgtggtgttc gacgagatca gcatggccac caactacgac
     1681 ctgtctgtgg tgaacgctag actgagggct aagcactacg tgtacatcgg tgacccagct
     1741 cagctgcctg ctcctcgtac cctgctgacc aagggcaccc tggagccaga atacttcaac
     1801 agcgtgtgcc gcctgatgaa gaccatcggt ccagacatgt tcctgggtac ctgccgccgc
     1861 tgccctgctg aaatcgtgga caccgtgtct gctctggtgt acgacaacaa gctgaaggcc
     1921 cacaaggaca agagcgccca gtgcttcaag atgttctaca agggagtgat cacccacgac
     1981 gtgtctagcg ccatcaacag gccccagatc ggtgtggtgc gcgagttcct gaccaggaac
     2041 cccgcctgga gaaaggccgt gttcatctcc ccttacaact ctcagaacgc cgtggcttcc
     2101 aagatcctgg gtctgcctac ccagaccgtg gactcctctc agggctctga atacgactac
     2161 gtgatcttca cccagaccac cgagaccgcc cacagctgca acgtgaacag gttcaacgtg
     2221 gccatcacca gagctaaagt gggaatcctg tgcatcatga gcgacagaga cctgtacgac
     2281 aagctgcagt tcacctccct ggagatcccc cgcaggaacg tggctaccct gcagtaccca
     2341 actttcttgt acaaagttgg cattataaga aagcattgct tatcaatttg ttgcaacgaa
     2401 caggtcacta tcagtcaaaa taaaatcatt atttgccatc cagctgatat cccctatagt
     2461 gagtcgtatt acatggtcat agctgtttcc tggcagctct ggcccgtgtc tcaaaatctc
     2521 tgatgttaca ttgcacaaga taaaaatata tcatcatgcc tcctctagac cagccaggac
     2581 agaaatgcct cgacttcgct gctacccaag gttgccgggt gacgcacacc gtggaaacgg
     2641 atgaaggcac gaacccagtg gacataagcc tgttcggttc gtaagctgta atgcaagtag
     2701 cgtatgcgct cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg taacggcgca
     2761 gtggcggttt tcatggcttg ttatgactgt ttttttgggg tacagtctat gcctcgggca
     2821 tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga tgttatggag cagcaacgat
     2881 gttacgcagc agggcagtcg ccctaaaaca aagttaaaca ttatgaggga agcggtgatc
     2941 gccgaagtat cgactcaact atcagaggta gttggcgtca tcgagcgcca tctcgaaccg
     3001 acgttgctgg ccgtacattt gtacggctcc gcagtggatg gcggcctgaa gccacacagt
     3061 gatattgatt tgctggttac ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg
     3121 atcaacgacc ttttggaaac ttcggcttcc cctggagaga gcgagattct ccgcgctgta
     3181 gaagtcacca ttgttgtgca cgacgacatc attccgtggc gttatccagc taagcgcgaa
     3241 ctgcaatttg gagaatggca gcgcaatgac attcttgcag gtatcttcga gccagccacg
     3301 atcgacattg atctggctat cttgctgaca aaagcaagag aacatagcgt tgccttggta
     3361 ggtccagcgg cggaggaact ctttgatccg gttcctgaac aggatctatt tgaggcgcta
     3421 aatgaaacct taacgctatg gaactcgccg cccgactggg ctggcgatga gcgaaatgta
     3481 gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat
     3541 gtcgctgccg actgggcaat ggagcgcctg ccggcccagt atcagcccgt catacttgaa
     3601 gctagacagg cttatcttgg acaagaagaa gatcgcttgg cctcgcgcgc agatcagttg
     3661 gaagaatttg tccactacgt gaaaggcgag atcaccaagg tagtcggcaa ataaccctcg
     3721 agccacccat gaccaaaatc ccttaacgtg agttacgcgt cgttccactg agcgtcagac
     3781 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     3841 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     3901 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     3961 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     4021 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     4081 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     4141 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     4201 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     4261 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     4321 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     4381 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     4441 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     4501 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     4561 agcgaggaag cggaagagcg cccaatacgc aaaccg
//
