LOCUS       sequence-296465-        4374 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector, with insert of NSP14 gene's CDS 
            from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4374)
  AUTHORS   Kim DK, Knapp JJ, Kuang D, Chawla A, Cassonnet P, Lee H, 
            Sheykhkarimli D, Samavarchi-Tehrani P, Abdouni H, Rayhan A, Li R, 
            Pogoutse O, Coyaud E, van der Werf S, Demeret C, Gingras AC, Taipale
            M, Raught B, Jacob Y, Roth FP
  TITLE     A Comprehensive, Flexible Collection of SARS-CoV-2 Coding Regions.
  JOURNAL   G3 (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554.
  PUBMED    32763951
REFERENCE   2  (bases 1 to 4374)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554."
COMMENT     Sequence Label: pDONR223 SARS-CoV-2 NSP14_nostop
FEATURES             Location/Qualifiers
     source          1..4374
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(63..90)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(182..268)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     317..339
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     331..348
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     332..348
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    364..463
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2048..2147)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2164..2183)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2165..2183)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2188..2204)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2188..2204)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2635..3426
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3519..4107
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4008..4027
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4261..4278
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ggcagtgagc gcaacgcaat taatacgcgt accgctagcc aggaagagtt tgtagaaacg
       61 caaaaaggcc atccgtcagg atggccttct gcttagtttg atgcctggca gtttatggcg
      121 ggcgtcctgc ccgccaccct ccgggccgtt gcttcacaac gttcaaatcc gctcccggcg
      181 gatttgtcct actcaggaga gcgttcaccg acaaacaaca gataaaacga aaggcccagt
      241 cttccgactg agcctttcgt tttatttgat gcctggcagt tccctactct cgcgttaacg
      301 ctagcatgga tgttttccca gtcacgacgt tgtaaaacga cggccagtct taagctcggg
      361 ccccaaataa tgattttatt ttgactgata gtgacctgtt cgttgcaaca aattgatgag
      421 caatgctttt ttataatgcc aactttgtac aaaaaagttg gcatggctga gaacgtgacc
      481 ggtctgttca aggactgctc taaggtcatc actggtctgc acccaactca ggctccaacc
      541 cacctgtctg tggacaccaa gttcaagacc gaaggactgt gcgtggacat ccccggtatc
      601 cctaaggaca tgacctaccg caggctgatc tccatgatgg gcttcaagat gaactaccag
      661 gtgaacggat accctaacat gttcatcacc agagaggaag ctatccgcca cgtgagggcc
      721 tggatcggtt tcgacgtgga aggttgccac gctaccagag aagctgtggg taccaacctg
      781 ccactgcagc tgggcttctc taccggagtg aacctggtgg ctgtgcctac cggctacgtg
      841 gacaccccaa acaacaccga cttctctaga gtgagcgcca agccccctcc aggagaccag
      901 ttcaagcacc tgatccccct gatgtacaag ggtctgcctt ggaacgtggt gcgcatcaag
      961 atcgtgcaga tgctgtccga caccctgaag aacctgtctg acagggtggt gttcgtgctg
     1021 tgggctcacg gcttcgagct gaccagcatg aagtacttcg tgaagatcgg acccgaacgt
     1081 acctgctgcc tgtgcgacag acgtgccacc tgcttctcca ccgcctctga cacctacgct
     1141 tgctggcacc actccatcgg attcgactac gtgtacaacc ctttcatgat cgacgtgcag
     1201 cagtggggat tcaccggtaa cctgcagtcc aaccacgacc tgtactgcca ggtgcacggt
     1261 aacgcccacg tggcttcttg cgacgctatc atgaccagat gcctggccgt gcacgagtgc
     1321 ttcgtgaagc gtgtggactg gaccatcgag taccctatca tcggcgacga actgaagatc
     1381 aacgccgctt gcagaaaggt gcagcacatg gtggtgaagg ccgctctgct ggctgacaag
     1441 ttcccagtgc tgcacgacat cggaaaccca aaggctatca agtgcgtgcc ccaggccgac
     1501 gtggagtgga agttctacga cgctcagccc tgctccgaca aggcctacaa gatcgaggaa
     1561 ctgttctact cttacgctac ccacagcgac aagttcaccg acggcgtgtg cctgttctgg
     1621 aactgcaacg tggacaggta cccagccaac tctatcgtgt gcagattcga cacccgtgtg
     1681 ctgagcaacc tgaacctgcc cggatgcgac ggcggatccc tgtacgtgaa caagcacgcc
     1741 ttccacaccc ctgctttcga caagtctgcc ttcgtgaacc tgaagcagct gcctttcttc
     1801 tactacagcg actccccatg cgagagccac ggcaagcagg tggtgtccga catcgactac
     1861 gtgcccctga agagcgccac ctgcatcacc cgctgcaacc tgggcggcgc cgtgtgcaga
     1921 caccacgcca acgaatacag gctgtacctg gacgcttaca acatgatgat cagcgccggc
     1981 ttctccctgt gggtgtacaa gcagttcgac acctacaacc tgtggaacac cttcaccagg
     2041 ctgcagtacc caactttctt gtacaaagtt ggcattataa gaaagcattg cttatcaatt
     2101 tgttgcaacg aacaggtcac tatcagtcaa aataaaatca ttatttgcca tccagctgat
     2161 atcccctata gtgagtcgta ttacatggtc atagctgttt cctggcagct ctggcccgtg
     2221 tctcaaaatc tctgatgtta cattgcacaa gataaaaata tatcatcatg cctcctctag
     2281 accagccagg acagaaatgc ctcgacttcg ctgctaccca aggttgccgg gtgacgcaca
     2341 ccgtggaaac ggatgaaggc acgaacccag tggacataag cctgttcggt tcgtaagctg
     2401 taatgcaagt agcgtatgcg ctcacgcaac tggtccagaa ccttgaccga acgcagcggt
     2461 ggtaacggcg cagtggcggt tttcatggct tgttatgact gtttttttgg ggtacagtct
     2521 atgcctcggg catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg
     2581 agcagcaacg atgttacgca gcagggcagt cgccctaaaa caaagttaaa cattatgagg
     2641 gaagcggtga tcgccgaagt atcgactcaa ctatcagagg tagttggcgt catcgagcgc
     2701 catctcgaac cgacgttgct ggccgtacat ttgtacggct ccgcagtgga tggcggcctg
     2761 aagccacaca gtgatattga tttgctggtt acggtgaccg taaggcttga tgaaacaacg
     2821 cggcgagctt tgatcaacga ccttttggaa acttcggctt cccctggaga gagcgagatt
     2881 ctccgcgctg tagaagtcac cattgttgtg cacgacgaca tcattccgtg gcgttatcca
     2941 gctaagcgcg aactgcaatt tggagaatgg cagcgcaatg acattcttgc aggtatcttc
     3001 gagccagcca cgatcgacat tgatctggct atcttgctga caaaagcaag agaacatagc
     3061 gttgccttgg taggtccagc ggcggaggaa ctctttgatc cggttcctga acaggatcta
     3121 tttgaggcgc taaatgaaac cttaacgcta tggaactcgc cgcccgactg ggctggcgat
     3181 gagcgaaatg tagtgcttac gttgtcccgc atttggtaca gcgcagtaac cggcaaaatc
     3241 gcgccgaagg atgtcgctgc cgactgggca atggagcgcc tgccggccca gtatcagccc
     3301 gtcatacttg aagctagaca ggcttatctt ggacaagaag aagatcgctt ggcctcgcgc
     3361 gcagatcagt tggaagaatt tgtccactac gtgaaaggcg agatcaccaa ggtagtcggc
     3421 aaataaccct cgagccaccc atgaccaaaa tcccttaacg tgagttacgc gtcgttccac
     3481 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     3541 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     3601 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     3661 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     3721 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     3781 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     3841 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     3901 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     3961 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     4021 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     4081 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     4141 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     4201 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     4261 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
     4321 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcg
//
