LOCUS       sequence-296467-        3687 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector, with insert of NSP16 gene's CDS 
            from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3687)
  AUTHORS   Kim DK, Knapp JJ, Kuang D, Chawla A, Cassonnet P, Lee H, 
            Sheykhkarimli D, Samavarchi-Tehrani P, Abdouni H, Rayhan A, Li R, 
            Pogoutse O, Coyaud E, van der Werf S, Demeret C, Gingras AC, Taipale
            M, Raught B, Jacob Y, Roth FP
  TITLE     A Comprehensive, Flexible Collection of SARS-CoV-2 Coding Regions.
  JOURNAL   G3 (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554.
  PUBMED    32763951
REFERENCE   2  (bases 1 to 3687)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554."
COMMENT     Sequence Label: pDONR223 SARS-CoV-2 NSP16_nostop
FEATURES             Location/Qualifiers
     source          1..3687
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(127..154)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(246..332)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     381..403
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     395..412
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     396..412
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    428..527
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1425..1524)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1541..1560)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1542..1560)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1565..1581)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1565..1581)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2012..2803
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2896..3484
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3385..3404
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3638..3655
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
       61 agcgggcagt gagcgcaacg caattaatac gcgtaccgct agccaggaag agtttgtaga
      121 aacgcaaaaa ggccatccgt caggatggcc ttctgcttag tttgatgcct ggcagtttat
      181 ggcgggcgtc ctgcccgcca ccctccgggc cgttgcttca caacgttcaa atccgctccc
      241 ggcggatttg tcctactcag gagagcgttc accgacaaac aacagataaa acgaaaggcc
      301 cagtcttccg actgagcctt tcgttttatt tgatgcctgg cagttcccta ctctcgcgtt
      361 aacgctagca tggatgtttt cccagtcacg acgttgtaaa acgacggcca gtcttaagct
      421 cgggccccaa ataatgattt tattttgact gatagtgacc tgttcgttgc aacaaattga
      481 tgagcaatgc ttttttataa tgccaacttt gtacaaaaaa gttggcatgt cttcccaggc
      541 ttggcagcct ggagtggcta tgcctaacct gtacaagatg cagcgtatgc tgctggagaa
      601 gtgcgacctg cagaactacg gagactccgc caccctgccc aagggtatca tgatgaacgt
      661 ggctaagtac acccagctgt gccagtacct gaacaccctg accctggccg tgccttacaa
      721 catgcgcgtg atccacttcg gtgctggttc tgacaaggga gtggctccag gtaccgctgt
      781 gctgagacag tggctgccca ccggtaccct gctggtggac tctgacctga acgacttcgt
      841 gtctgatgct gacagcaccc tgatcggcga ctgtgctact gtgcacaccg ctaacaagtg
      901 ggacctgatc atctctgaca tgtacgaccc aaagaccaag aacgtgacca aggagaacga
      961 cagcaaggaa ggtttcttca cctacatctg cggcttcatc cagcagaagc tggccctggg
     1021 cggatctgtg gctatcaaga tcaccgaaca ctcctggaac gctgacctgt acaagctgat
     1081 gggacacttc gcctggtgga ccgctttcgt gaccaacgtg aacgcctcta gctccgaggc
     1141 tttcctgatc ggctgcaact acctgggaaa gccccgtgaa cagatcgacg gttacgtgat
     1201 gcacgccaac tacatcttct ggcgcaacac caaccctatc cagctgtcta gctactccct
     1261 gttcgacatg tctaagttcc cactgaagct gagaggcacc gctgtgatga gcctgaagga
     1321 gggacagatc aacgacatga tcctgagcct gctgtccaag ggacgcctga tcatcaggga
     1381 aaacaacaga gtggtcatct cctctgacgt gctggtgaac aactacccaa ctttcttgta
     1441 caaagttggc attataagaa agcattgctt atcaatttgt tgcaacgaac aggtcactat
     1501 cagtcaaaat aaaatcatta tttgccatcc agctgatatc ccctatagtg agtcgtatta
     1561 catggtcata gctgtttcct ggcagctctg gcccgtgtct caaaatctct gatgttacat
     1621 tgcacaagat aaaaatatat catcatgcct cctctagacc agccaggaca gaaatgcctc
     1681 gacttcgctg ctacccaagg ttgccgggtg acgcacaccg tggaaacgga tgaaggcacg
     1741 aacccagtgg acataagcct gttcggttcg taagctgtaa tgcaagtagc gtatgcgctc
     1801 acgcaactgg tccagaacct tgaccgaacg cagcggtggt aacggcgcag tggcggtttt
     1861 catggcttgt tatgactgtt tttttggggt acagtctatg cctcgggcat ccaagcagca
     1921 agcgcgttac gccgtgggtc gatgtttgat gttatggagc agcaacgatg ttacgcagca
     1981 gggcagtcgc cctaaaacaa agttaaacat tatgagggaa gcggtgatcg ccgaagtatc
     2041 gactcaacta tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc
     2101 cgtacatttg tacggctccg cagtggatgg cggcctgaag ccacacagtg atattgattt
     2161 gctggttacg gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga tcaacgacct
     2221 tttggaaact tcggcttccc ctggagagag cgagattctc cgcgctgtag aagtcaccat
     2281 tgttgtgcac gacgacatca ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg
     2341 agaatggcag cgcaatgaca ttcttgcagg tatcttcgag ccagccacga tcgacattga
     2401 tctggctatc ttgctgacaa aagcaagaga acatagcgtt gccttggtag gtccagcggc
     2461 ggaggaactc tttgatccgg ttcctgaaca ggatctattt gaggcgctaa atgaaacctt
     2521 aacgctatgg aactcgccgc ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt
     2581 gtcccgcatt tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga
     2641 ctgggcaatg gagcgcctgc cggcccagta tcagcccgtc atacttgaag ctagacaggc
     2701 ttatcttgga caagaagaag atcgcttggc ctcgcgcgca gatcagttgg aagaatttgt
     2761 ccactacgtg aaaggcgaga tcaccaaggt agtcggcaaa taaccctcga gccacccatg
     2821 accaaaatcc cttaacgtga gttacgcgtc gttccactga gcgtcagacc ccgtagaaaa
     2881 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     2941 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     3001 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     3061 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     3121 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     3181 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     3241 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     3301 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     3361 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     3421 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     3481 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     3541 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     3601 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     3661 ggaagagcgc ccaatacgca aaccgcc
//
