LOCUS       sequence-296468-        4195 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector, with insert of ORF3A gene's CDS 
            from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4195)
  AUTHORS   Kim DK, Knapp JJ, Kuang D, Chawla A, Cassonnet P, Lee H, 
            Sheykhkarimli D, Samavarchi-Tehrani P, Abdouni H, Rayhan A, Li R, 
            Pogoutse O, Coyaud E, van der Werf S, Demeret C, Gingras AC, Taipale
            M, Raught B, Jacob Y, Roth FP
  TITLE     A Comprehensive, Flexible Collection of SARS-CoV-2 Coding Regions.
  JOURNAL   G3 (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554.
  PUBMED    32763951
REFERENCE   2  (bases 1 to 4195)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554."
COMMENT     Sequence Label: pDONR207 SARS-CoV-2 ORF3A_nostop
FEATURES             Location/Qualifiers
     source          1..4195
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(67..94)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(186..272)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    325..424
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1250..1349)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1461..1480)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(1539..1558)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     CDS             complement(2132..2665)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(2854..2859,2860..2876,2877..2882))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     rep_origin      3539..4127
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4028..4047
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgcgttatcc cctgattctg tggataaccg tattaccgct agccaggaag agtttgtaga
       61 aacgcaaaaa ggccatccgt caggatggcc ttctgcttag tttgatgcct ggcagtttat
      121 ggcgggcgtc ctgcccgcca ccctccgggc cgttgcttca caacgttcaa atccgctccc
      181 ggcggatttg tcctactcag gagagcgttc accgacaaac aacagataaa acgaaaggcc
      241 cagtcttccg actgagcctt tcgttttatt tgatgcctgg cagttcccta ctctcgcgtt
      301 aacgctagca tggatctcgg gccccaaata atgattttat tttgactgat agtgacctgt
      361 tcgttgcaac aaattgatga gcaatgcttt tttataatgc caactttgta caaaaaagtt
      421 ggcatggacc tgttcatgag aatcttcacc atcggcaccg tgaccctgaa gcagggagag
      481 atcaaggacg ccaccccttc tgacttcgtg cgtgccaccg ctaccatccc aatccaggct
      541 agcctgccct tcggatggct gatcgtgggt gtggccctgc tggccgtgtt ccagtccgcc
      601 tctaagatca tcaccctgaa gaagaggtgg cagctggctc tgtccaaggg tgtgcacttc
      661 gtgtgcaacc tgctgctgct gttcgtgacc gtgtactcac acctgctgct ggtggctgct
      721 ggtctggagg ctccattcct gtacctgtac gctctggtgt acttcctgca gagcatcaac
      781 ttcgtgcgca tcatcatgag gctgtggctg tgctggaagt gcagatccaa gaaccccctg
      841 ctgtacgacg ccaactactt cctgtgctgg cacaccaact gctacgacta ctgcatcccc
      901 tacaacagcg tgaccagctc catcgtgatc acctccggtg acggcaccac cagccctatc
      961 tccgaacacg actaccagat cggcggatac accgagaagt gggaatctgg agtgaaggac
     1021 tgcgtggtgc tgcactctta cttcaccagc gactactacc agctgtactc cacccagctg
     1081 tctaccgaca ccggtgtgga gcacgtgacc ttcttcatct acaacaagat cgtggacgaa
     1141 cccgaggaac acgtgcagat ccacaccatc gacgtgtcta gcggcgtggt gaaccccgtg
     1201 atggagccta tctacgacga acctaccacc accacctccg tgccactgta cccaactttc
     1261 ttgtacaaag ttggcattat aagaaagcat tgcttatcaa tttgttgcaa cgaacaggtc
     1321 actatcagtc aaaataaaat cattatttgc catccagctg cagctctggc ccgtgtctca
     1381 aaatctctga tgttacattg cacaagataa aaatatatca tcatgaacaa taaaactgtc
     1441 tgcttacata aacagtaata caaggggtgt tatgagccat attcaacggg aaacgtcgag
     1501 gccgcgatta aattccaaca tggatgctga tttatatggg tataaatggg ctcgcgataa
     1561 tgtcgggcaa tcaggtgcga caatctatcg cttgtatggg aagcccgatg cgccagagtt
     1621 gtttctgaaa catggcaaag gtagcgttgc caatgatgtt acagatgaga tggtcagact
     1681 aaactggctg acggaattta tgcctcttcc gaccatcaag cattttatcc gtactcctga
     1741 tgatgcatgg ttactcacca ctgcgatccc cggaaaaaca gcattccagg tattagaaga
     1801 atatcctgat tcaggtgaaa atattgttga tgcgctggca gtgttcctgc gccggttgca
     1861 ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc
     1921 gcaatcacga atgaataacg gtttggttga tgcgagtgat tttgatgacg agcgtaatgg
     1981 ctggcctgtt gaacaagtct ggaaagaaat gcataaactt ttgccattct caccggattc
     2041 agtcgtcact catggtgatt tctcacttga taaccttatt tttgacgagg ggaaattagg
     2101 ccgggaagcc gatctcggct tgaacgaatt gttaggtggc ggtacttggg tcgatatcaa
     2161 agtgcatcac ttcttcccgt atgcccaact ttgtatagag agccactgcg ggatcgtcac
     2221 cgtaatctgc ttgcacgtag atcacataag caccaagcgc gttggcctca tgcttgagga
     2281 gattgatgag cgcggtggca atgccctgcc tccggtgctc gccggagact gcgagatcat
     2341 agatatagat ctcactacgc ggctgctcaa acttgggcag aacgtaagcc gcgagagcgc
     2401 caacaaccgc ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta cggagcaagt
     2461 tcccgaggta atcggagtcc ggctgatgtt gggagtaggt ggctacgtct ccgaactcac
     2521 gaccgaaaag atcaagagca gcccgcatgg atttgacttg gtcagggccg agcctacatg
     2581 tgcgaatgat gcccatactt gagccaccta actttgtttt agggcgactg ccctgctgcg
     2641 taacatcgtt gctgctgcgt aacatcgttg ctgctccata acatcaaaca tcgacccacg
     2701 gcgtaacgcg cttgctgctt ggatgcccga ggcatagact gtacaaaaaa acagtcataa
     2761 caagccatga aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa ggttctggac
     2821 cagttgcgtg agcgcatacg ctacttgcat tacagtttac gaaccgaaca ggcttatgtc
     2881 aactgggttc gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac cttgggcagc
     2941 agcgaagtcg aggcatttct gtcctggctg gcgaacgagc gcaaggtttc ggtctccacg
     3001 catcgtcagg cattggcggc cttgctgttc ttctacggca aggtgctgtg cacggatctg
     3061 ccctggcttc aggagatcgg aagacctcgg ccgtcgcggc gcttgccggt ggtgctgacc
     3121 ccggatgaag tggttcgcat cctcggtttt ctggaaggcg agcatcgttt gttcgcccag
     3181 gactctagct atagttctag tggttggcta ctaataggtt gtattgatgt tggacgagtc
     3241 ggaatcgcag accgatacca ggatcttgcc atcctatgga actgcctcgg tgagttttct
     3301 ccttcattac agaaacggct ttttcaaaaa tatggtattg ataatcctga tatgaataaa
     3361 ttgcagtttc atttgatgct cgatgagttt ttctaatcag aattggttaa ttggttgtaa
     3421 cactggcaga gcattacgct gacttgacgg gacggcgcaa gctcatgacc aaaatccctt
     3481 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     3541 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     3601 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     3661 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     3721 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     3781 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     3841 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     3901 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     3961 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     4021 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     4081 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     4141 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcc
//
