LOCUS       sequence-296470-        3595 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector, with insert of the envelope (E) 
            gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop
            codon..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3595)
  AUTHORS   Kim DK, Knapp JJ, Kuang D, Chawla A, Cassonnet P, Lee H, 
            Sheykhkarimli D, Samavarchi-Tehrani P, Abdouni H, Rayhan A, Li R, 
            Pogoutse O, Coyaud E, van der Werf S, Demeret C, Gingras AC, Taipale
            M, Raught B, Jacob Y, Roth FP
  TITLE     A Comprehensive, Flexible Collection of SARS-CoV-2 Coding Regions.
  JOURNAL   G3 (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554.
  PUBMED    32763951
REFERENCE   2  (bases 1 to 3595)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554."
COMMENT     Sequence Label: pDONR207 SARS-CoV-2 E_nostop
FEATURES             Location/Qualifiers
     source          1..3595
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(52..79)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(171..257)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    310..409
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(635..734)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(846..865)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(924..943)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     CDS             complement(1517..2050)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(2239..2244,2245..2261,2262..2267))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     rep_origin      2924..3512
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3413..3432
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttctgtggat aaccgtatta ccgctagcca ggaagagttt gtagaaacgc aaaaaggcca
       61 tccgtcagga tggccttctg cttagtttga tgcctggcag tttatggcgg gcgtcctgcc
      121 cgccaccctc cgggccgttg cttcacaacg ttcaaatccg ctcccggcgg atttgtccta
      181 ctcaggagag cgttcaccga caaacaacag ataaaacgaa aggcccagtc ttccgactga
      241 gcctttcgtt ttatttgatg cctggcagtt ccctactctc gcgttaacgc tagcatggat
      301 ctcgggcccc aaataatgat tttattttga ctgatagtga cctgttcgtt gcaacaaatt
      361 gatgagcaat gcttttttat aatgccaact ttgtacaaaa aagttggcat gtactctttc
      421 gtgagcgagg aaaccggcac cctgatcgtg aactccgtgc tgctgttcct ggccttcgtg
      481 gtgttcctgc tggtgaccct ggctatcctg accgctctga gactgtgcgc ttactgctgc
      541 aacatcgtga acgtgtccct ggtgaagccc tctttctacg tgtacagccg cgtgaagaac
      601 ctgaacagct ccagggtgcc tgacctgctg gtgtacccaa ctttcttgta caaagttggc
      661 attataagaa agcattgctt atcaatttgt tgcaacgaac aggtcactat cagtcaaaat
      721 aaaatcatta tttgccatcc agctgcagct ctggcccgtg tctcaaaatc tctgatgtta
      781 cattgcacaa gataaaaata tatcatcatg aacaataaaa ctgtctgctt acataaacag
      841 taatacaagg ggtgttatga gccatattca acgggaaacg tcgaggccgc gattaaattc
      901 caacatggat gctgatttat atgggtataa atgggctcgc gataatgtcg ggcaatcagg
      961 tgcgacaatc tatcgcttgt atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg
     1021 caaaggtagc gttgccaatg atgttacaga tgagatggtc agactaaact ggctgacgga
     1081 atttatgcct cttccgacca tcaagcattt tatccgtact cctgatgatg catggttact
     1141 caccactgcg atccccggaa aaacagcatt ccaggtatta gaagaatatc ctgattcagg
     1201 tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg
     1261 taattgtcct tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa
     1321 taacggtttg gttgatgcga gtgattttga tgacgagcgt aatggctggc ctgttgaaca
     1381 agtctggaaa gaaatgcata aacttttgcc attctcaccg gattcagtcg tcactcatgg
     1441 tgatttctca cttgataacc ttatttttga cgaggggaaa ttaggccggg aagccgatct
     1501 cggcttgaac gaattgttag gtggcggtac ttgggtcgat atcaaagtgc atcacttctt
     1561 cccgtatgcc caactttgta tagagagcca ctgcgggatc gtcaccgtaa tctgcttgca
     1621 cgtagatcac ataagcacca agcgcgttgg cctcatgctt gaggagattg atgagcgcgg
     1681 tggcaatgcc ctgcctccgg tgctcgccgg agactgcgag atcatagata tagatctcac
     1741 tacgcggctg ctcaaacttg ggcagaacgt aagccgcgag agcgccaaca accgcttctt
     1801 ggtcgaaggc agcaagcgcg atgaatgtct tactacggag caagttcccg aggtaatcgg
     1861 agtccggctg atgttgggag taggtggcta cgtctccgaa ctcacgaccg aaaagatcaa
     1921 gagcagcccg catggatttg acttggtcag ggccgagcct acatgtgcga atgatgccca
     1981 tacttgagcc acctaacttt gttttagggc gactgccctg ctgcgtaaca tcgttgctgc
     2041 tgcgtaacat cgttgctgct ccataacatc aaacatcgac ccacggcgta acgcgcttgc
     2101 tgcttggatg cccgaggcat agactgtaca aaaaaacagt cataacaagc catgaaaacc
     2161 gccactgcgc cgttaccacc gctgcgttcg gtcaaggttc tggaccagtt gcgtgagcgc
     2221 atacgctact tgcattacag tttacgaacc gaacaggctt atgtcaactg ggttcgtgcc
     2281 ttcatccgtt tccacggtgt gcgtcacccg gcaaccttgg gcagcagcga agtcgaggca
     2341 tttctgtcct ggctggcgaa cgagcgcaag gtttcggtct ccacgcatcg tcaggcattg
     2401 gcggccttgc tgttcttcta cggcaaggtg ctgtgcacgg atctgccctg gcttcaggag
     2461 atcggaagac ctcggccgtc gcggcgcttg ccggtggtgc tgaccccgga tgaagtggtt
     2521 cgcatcctcg gttttctgga aggcgagcat cgtttgttcg cccaggactc tagctatagt
     2581 tctagtggtt ggctactaat aggttgtatt gatgttggac gagtcggaat cgcagaccga
     2641 taccaggatc ttgccatcct atggaactgc ctcggtgagt tttctccttc attacagaaa
     2701 cggctttttc aaaaatatgg tattgataat cctgatatga ataaattgca gtttcatttg
     2761 atgctcgatg agtttttcta atcagaattg gttaattggt tgtaacactg gcagagcatt
     2821 acgctgactt gacgggacgg cgcaagctca tgaccaaaat cccttaacgt gagttttcgt
     2881 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     2941 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     3001 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     3061 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     3121 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     3181 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     3241 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     3301 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     3361 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     3421 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     3481 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     3541 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctga
//
