LOCUS       sequence-296471-        4036 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector, with insert of the membrane (M) 
            gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop
            codon..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4036)
  AUTHORS   Kim DK, Knapp JJ, Kuang D, Chawla A, Cassonnet P, Lee H, 
            Sheykhkarimli D, Samavarchi-Tehrani P, Abdouni H, Rayhan A, Li R, 
            Pogoutse O, Coyaud E, van der Werf S, Demeret C, Gingras AC, Taipale
            M, Raught B, Jacob Y, Roth FP
  TITLE     A Comprehensive, Flexible Collection of SARS-CoV-2 Coding Regions.
  JOURNAL   G3 (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554.
  PUBMED    32763951
REFERENCE   2  (bases 1 to 4036)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554."
COMMENT     Sequence Label: pDONR207 SARS-CoV-2 M_nostop
FEATURES             Location/Qualifiers
     source          1..4036
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(113..199)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    252..351
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1018..1117)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1229..1248)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(1307..1326)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     CDS             complement(1900..2433)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(2622..2627,2628..2644,2645..2650))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     rep_origin      3307..3895
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3796..3815
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 catcctgacg gatggccttc tgcttagttt gatgcctggc agtttatggc gggcgtcctg
       61 cccgccaccc tccgggccgt tgcttcacaa cgttcaaatc cgctcccggc ggatttgtcc
      121 tactcaggag agcgttcacc gacaaacaac agataaaacg aaaggcccag tcttccgact
      181 gagcctttcg ttttatttga tgcctggcag ttccctactc tcgcgttaac gctagcatgg
      241 atctcgggcc ccaaataatg attttatttt gactgatagt gacctgttcg ttgcaacaaa
      301 ttgatgagca atgctttttt ataatgccaa ctttgtacaa aaaagttggc atggctgact
      361 ctaacggtac catcaccgtg gaggaactga agaagctgct ggagcagtgg aacctggtca
      421 tcggcttcct gttcctgacc tggatctgcc tgctgcagtt cgcctacgct aaccgcaaca
      481 ggttcctgta catcatcaag ctgatcttcc tgtggctgct gtggcctgtg accctggctt
      541 gcttcgtgct ggctgccgtg taccgcatca actggatcac cggcggaatc gccatcgcta
      601 tggcctgcct ggtgggcctg atgtggctgt cttacttcat cgctagcttc aggctgttcg
      661 ccagaacccg ttccatgtgg tctttcaacc ccgagaccaa catcctgctg aacgtgcctc
      721 tgcacggaac catcctgacc agaccactgc tggagagcga actggtcatc ggcgctgtga
      781 tcctgagagg acacctgcgt atcgccggac accacctggg tcgttgcgac atcaaggacc
      841 tgcccaagga aatcaccgtg gctaccagcc gcaccctgtc ctactacaag ctgggagctt
      901 ctcagagagt ggctggtgac tctggtttcg ctgcttactc tcgctacagg atcggtaact
      961 acaagctgaa caccgaccac agctcctcta gcgacaacat cgccctgctg gtgcagtacc
     1021 caactttctt gtacaaagtt ggcattataa gaaagcattg cttatcaatt tgttgcaacg
     1081 aacaggtcac tatcagtcaa aataaaatca ttatttgcca tccagctgca gctctggccc
     1141 gtgtctcaaa atctctgatg ttacattgca caagataaaa atatatcatc atgaacaata
     1201 aaactgtctg cttacataaa cagtaataca aggggtgtta tgagccatat tcaacgggaa
     1261 acgtcgaggc cgcgattaaa ttccaacatg gatgctgatt tatatgggta taaatgggct
     1321 cgcgataatg tcgggcaatc aggtgcgaca atctatcgct tgtatgggaa gcccgatgcg
     1381 ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca atgatgttac agatgagatg
     1441 gtcagactaa actggctgac ggaatttatg cctcttccga ccatcaagca ttttatccgt
     1501 actcctgatg atgcatggtt actcaccact gcgatccccg gaaaaacagc attccaggta
     1561 ttagaagaat atcctgattc aggtgaaaat attgttgatg cgctggcagt gttcctgcgc
     1621 cggttgcatt cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt atttcgtctc
     1681 gctcaggcgc aatcacgaat gaataacggt ttggttgatg cgagtgattt tgatgacgag
     1741 cgtaatggct ggcctgttga acaagtctgg aaagaaatgc ataaactttt gccattctca
     1801 ccggattcag tcgtcactca tggtgatttc tcacttgata accttatttt tgacgagggg
     1861 aaattaggcc gggaagccga tctcggcttg aacgaattgt taggtggcgg tacttgggtc
     1921 gatatcaaag tgcatcactt cttcccgtat gcccaacttt gtatagagag ccactgcggg
     1981 atcgtcaccg taatctgctt gcacgtagat cacataagca ccaagcgcgt tggcctcatg
     2041 cttgaggaga ttgatgagcg cggtggcaat gccctgcctc cggtgctcgc cggagactgc
     2101 gagatcatag atatagatct cactacgcgg ctgctcaaac ttgggcagaa cgtaagccgc
     2161 gagagcgcca acaaccgctt cttggtcgaa ggcagcaagc gcgatgaatg tcttactacg
     2221 gagcaagttc ccgaggtaat cggagtccgg ctgatgttgg gagtaggtgg ctacgtctcc
     2281 gaactcacga ccgaaaagat caagagcagc ccgcatggat ttgacttggt cagggccgag
     2341 cctacatgtg cgaatgatgc ccatacttga gccacctaac tttgttttag ggcgactgcc
     2401 ctgctgcgta acatcgttgc tgctgcgtaa catcgttgct gctccataac atcaaacatc
     2461 gacccacggc gtaacgcgct tgctgcttgg atgcccgagg catagactgt acaaaaaaac
     2521 agtcataaca agccatgaaa accgccactg cgccgttacc accgctgcgt tcggtcaagg
     2581 ttctggacca gttgcgtgag cgcatacgct acttgcatta cagtttacga accgaacagg
     2641 cttatgtcaa ctgggttcgt gccttcatcc gtttccacgg tgtgcgtcac ccggcaacct
     2701 tgggcagcag cgaagtcgag gcatttctgt cctggctggc gaacgagcgc aaggtttcgg
     2761 tctccacgca tcgtcaggca ttggcggcct tgctgttctt ctacggcaag gtgctgtgca
     2821 cggatctgcc ctggcttcag gagatcggaa gacctcggcc gtcgcggcgc ttgccggtgg
     2881 tgctgacccc ggatgaagtg gttcgcatcc tcggttttct ggaaggcgag catcgtttgt
     2941 tcgcccagga ctctagctat agttctagtg gttggctact aataggttgt attgatgttg
     3001 gacgagtcgg aatcgcagac cgataccagg atcttgccat cctatggaac tgcctcggtg
     3061 agttttctcc ttcattacag aaacggcttt ttcaaaaata tggtattgat aatcctgata
     3121 tgaataaatt gcagtttcat ttgatgctcg atgagttttt ctaatcagaa ttggttaatt
     3181 ggttgtaaca ctggcagagc attacgctga cttgacggga cggcgcaagc tcatgaccaa
     3241 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     3301 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     3361 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     3421 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     3481 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     3541 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     3601 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     3661 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     3721 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     3781 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     3841 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     3901 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     3961 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gctagccagg aagagtttgt
     4021 agaaacgcaa aaaggc
//
