LOCUS       sequence-296477-        3081 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector, with insert of ORF9B gene's CDS 
            from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3081)
  AUTHORS   Kim DK, Knapp JJ, Kuang D, Chawla A, Cassonnet P, Lee H, 
            Sheykhkarimli D, Samavarchi-Tehrani P, Abdouni H, Rayhan A, Li R, 
            Pogoutse O, Coyaud E, van der Werf S, Demeret C, Gingras AC, Taipale
            M, Raught B, Jacob Y, Roth FP
  TITLE     A Comprehensive, Flexible Collection of SARS-CoV-2 Coding Regions.
  JOURNAL   G3 (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554.
  PUBMED    32763951
REFERENCE   2  (bases 1 to 3081)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554."
COMMENT     Sequence Label: pDONR223 SARS-CoV-2 ORF9B_nostop
FEATURES             Location/Qualifiers
     source          1..3081
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(55..141)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     190..212
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     204..221
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     205..221
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    237..336
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(628..727)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(744..763)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(745..763)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(768..784)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(768..784)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             1215..2006
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2099..2687
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2588..2607
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2841..2858
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3017..3044)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
ORIGIN
        1 tgcccgccac cctccgggcc gttgcttcac aacgttcaaa tccgctcccg gcggatttgt
       61 cctactcagg agagcgttca ccgacaaaca acagataaaa cgaaaggccc agtcttccga
      121 ctgagccttt cgttttattt gatgcctggc agttccctac tctcgcgtta acgctagcat
      181 ggatgttttc ccagtcacga cgttgtaaaa cgacggccag tcttaagctc gggccccaaa
      241 taatgatttt attttgactg atagtgacct gttcgttgca acaaattgat gagcaatgct
      301 tttttataat gccaactttg tacaaaaaag ttggcatgga cccaaagatc tctgaaatgc
      361 accccgctct gagactggtg gaccctcaga tccagctggc cgtgacccgc atggaaaacg
      421 ctgtgggcag ggaccagaac aacgtgggac ccaaggtgta ccctatcatc ctgagactgg
      481 gcagccctct gtccctgaac atggcccgta agaccctgaa cagcctggag gacaaggcct
      541 tccagctgac cccaatcgct gtgcagatga ccaagctggc caccaccgag gaactgcccg
      601 acgaattcgt ggtggtgacc gtgaagtacc caactttctt gtacaaagtt ggcattataa
      661 gaaagcattg cttatcaatt tgttgcaacg aacaggtcac tatcagtcaa aataaaatca
      721 ttatttgcca tccagctgat atcccctata gtgagtcgta ttacatggtc atagctgttt
      781 cctggcagct ctggcccgtg tctcaaaatc tctgatgtta cattgcacaa gataaaaata
      841 tatcatcatg cctcctctag accagccagg acagaaatgc ctcgacttcg ctgctaccca
      901 aggttgccgg gtgacgcaca ccgtggaaac ggatgaaggc acgaacccag tggacataag
      961 cctgttcggt tcgtaagctg taatgcaagt agcgtatgcg ctcacgcaac tggtccagaa
     1021 ccttgaccga acgcagcggt ggtaacggcg cagtggcggt tttcatggct tgttatgact
     1081 gtttttttgg ggtacagtct atgcctcggg catccaagca gcaagcgcgt tacgccgtgg
     1141 gtcgatgttt gatgttatgg agcagcaacg atgttacgca gcagggcagt cgccctaaaa
     1201 caaagttaaa cattatgagg gaagcggtga tcgccgaagt atcgactcaa ctatcagagg
     1261 tagttggcgt catcgagcgc catctcgaac cgacgttgct ggccgtacat ttgtacggct
     1321 ccgcagtgga tggcggcctg aagccacaca gtgatattga tttgctggtt acggtgaccg
     1381 taaggcttga tgaaacaacg cggcgagctt tgatcaacga ccttttggaa acttcggctt
     1441 cccctggaga gagcgagatt ctccgcgctg tagaagtcac cattgttgtg cacgacgaca
     1501 tcattccgtg gcgttatcca gctaagcgcg aactgcaatt tggagaatgg cagcgcaatg
     1561 acattcttgc aggtatcttc gagccagcca cgatcgacat tgatctggct atcttgctga
     1621 caaaagcaag agaacatagc gttgccttgg taggtccagc ggcggaggaa ctctttgatc
     1681 cggttcctga acaggatcta tttgaggcgc taaatgaaac cttaacgcta tggaactcgc
     1741 cgcccgactg ggctggcgat gagcgaaatg tagtgcttac gttgtcccgc atttggtaca
     1801 gcgcagtaac cggcaaaatc gcgccgaagg atgtcgctgc cgactgggca atggagcgcc
     1861 tgccggccca gtatcagccc gtcatacttg aagctagaca ggcttatctt ggacaagaag
     1921 aagatcgctt ggcctcgcgc gcagatcagt tggaagaatt tgtccactac gtgaaaggcg
     1981 agatcaccaa ggtagtcggc aaataaccct cgagccaccc atgaccaaaa tcccttaacg
     2041 tgagttacgc gtcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     2101 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     2161 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     2221 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     2281 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     2341 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     2401 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     2461 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     2521 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     2581 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     2641 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     2701 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     2761 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     2821 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     2881 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
     2941 ccgactggaa agcgggcagt gagcgcaacg caattaatac gcgtaccgct agccaggaag
     3001 agtttgtaga aacgcaaaaa ggccatccgt caggatggcc ttctgcttag tttgatgcct
     3061 ggcagtttat ggcgggcgtc c
//
