LOCUS       sequence-296480-        2417 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Truncated mouse gamma-synuclein (mSncg-0.27kb) promoter-mediates 
            gene expression in retinal ganglion cells with fluorescent reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2417)
  AUTHORS   Wang Q, Zhuang PS, Huang H, Li L, Liu L, Webber HC, Dalal R, Siew L,
            Fligor CM, Chang KC, Nahmou M, Kreymerman A, Sun Y, Meyer JS, 
            Goldberg JL, Hu Y
  TITLE     Mouse gamma-Synuclein Promoter-Mediated Gene Expression and Editing 
            in Mammalian Retinal Ganglion Cells.
  JOURNAL   J Neurosci. 2020 Apr 9. pii: JNEUROSCI.0102-20.2020. doi: 
            10.1523/JNEUROSCI.0102-20.2020.
  PUBMED    32300046
REFERENCE   2  (bases 1 to 2417)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Neurosci.
            2020 Apr 9. pii: JNEUROSCI.0102-20.2020. doi: 
            10.1523/JNEUROSCI.0102-20.2020."
COMMENT     Sequence Label: pAM-AAV-mSncg-0.27kb-EGFP
FEATURES             Location/Qualifiers
     source          1..2417
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     repeat_region   1..130
                     /label=AAV2 ITR
     CDS             join(580..582,583..585,586..1299)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(580..582,583..585,586..1296)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(625..646)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(886..905)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1233..1254
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    1357..1945
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1410..1430)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1828..1839)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(1972..1989)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1978..2202
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   2288..2417
                     /label=AAV2 ITR
ORIGIN
        1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct tgtagttaat gattaacccg ccatgctact tatttacgta gccatgctct
      181 aggtaccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      241 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      301 atcatatgaa gagtcagcag ggcagaatag agccagtagg ggcccgggcc ctgctcgctg
      361 gtatccccgt gaggcatgcc ttctctctgg cccgccctcc ctgcccccac cctggcccgg
      421 gctggctggg ctccagccag cagccacagc atcaatattt catctgcgtc aataagaggc
      481 agtagcagca gagacagcgg ctgcggcagc actccagtcc atagcttgca gcagccaggt
      541 tccatccttg caaacaccat ggacgtcttc aaggaattca tggtgagcaa gggcgaggag
      601 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag
      661 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc
      721 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac
      781 ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc
      841 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac
      901 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
      961 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac
     1021 agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag
     1081 atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc
     1141 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc
     1201 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc
     1261 gccgggatca ctctcggcat ggacgagctg tacaagtaaa gatctacgcg tggtacctct
     1321 agagtcgacc cgggcggcct cgagaagctt atcgataatc aacctctgga ttacaaaatt
     1381 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     1441 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     1501 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     1561 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     1621 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     1681 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     1741 ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg
     1801 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     1861 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
     1921 atctcccttt gggccgcctc cccgcatcga taccgtcgac tcgctgatca gcctcgactg
     1981 tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg
     2041 aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga
     2101 gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg
     2161 aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa
     2221 ccagctgggg ctcgactaga gcatggctac gtagataagt agcatggcgg gttaatcatt
     2281 aactacaagg aacccctagt gatggagttg gccactccct ctctgcgcgc tcgctcgctc
     2341 actgaggccg ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg
     2401 agcgagcgag cgcgcag
//
