LOCUS       sequence-296488-        5681 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bait vector AT1G07550 L10_pECIA2 should be used with prey vector 
            AT1G07550 L10_pECIA14..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5681)
  AUTHORS   Smakowska-Luzan E, Mott GA, Parys K, Stegmann M, Howton TC, 
            Layeghifard M, Neuhold J, Lehner A, Kong J, Grunwald K, Weinberger 
            N, Satbhai SB, Mayer D, Busch W, Madalinski M, Stolt-Bergner P, 
            Provart NJ, Mukhtar MS, Zipfel C, Desveaux D, Guttman DS, Belkhadir 
            Y
  TITLE     An extracellular network of Arabidopsis leucine-rich repeat receptor
            kinases.
  JOURNAL   Nature. 2018 Jan 18;553(7688):342-346. doi: 10.1038/nature25184. 
            Epub 2018 Jan 10.
  PUBMED    29320478
REFERENCE   2  (bases 1 to 5681)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature25184"; journalName: "Nature"; date: "2018-01-18- 
            18"; volume: "553"; issue: "7688"; pages: "342-346"
COMMENT     Sequence Label: AT1G07550 L10_pECIA2
FEATURES             Location/Qualifiers
     source          1..5681
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        23..449
                     /label=MT promoter
                     /note="Drosophila metallothionein promoter"
     sig_peptide     467..520
                     /codon_start=1
                     /product="Drosophila BiP signal sequence"
                     /label=BiP signal sequence
                     /translation="MKLCILLAVVAFVGLSLG"
     CDS             1934..1957
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             2663..2704
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             2714..2731
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(2754..2771)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2804..2938
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2828..2847)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2882..2901
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(3082..3098)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3082..3098)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3095..3117)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3106..3122
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3130..3136,3137..3154,3155..3160))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3175..3196
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3313..3330)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3484..4072)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3564..3583)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4243..5034,5035..5103))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4866..4885
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5104..5208)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5276..5294
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(5332..5354)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5454..5473
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     join(5667..5681,1..8)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 gtaaaacgac ggccagtgaa ttaattcgtt gcaggacagg atgtggtgcc cgatgtgact
       61 agctctttgc tgcaggccgt cctatcctct ggttccgata agagacccag aactccggcc
      121 ccccaccgcc caccgccacc cccatacata tgtggtacgc aagtaagagt gcctgcgcat
      181 gccccatgtg ccccaccaag agctttgcat cccatacaag tccccaaagt ggagaaccga
      241 accaattctt cgcgggcaga acaaaagctt ctgcacacgt ctccactcga atttggagcc
      301 ggccggcgtg tgcaaaagag gtgaatcgaa cgaaagaccc gtgtgtaaag ccgcgtttcc
      361 aaaatgtata aaaccgagag catctggcca atgtgcatca gttgtggtca gcagcaaaat
      421 caagtgaatc atctcagtgc aactaaaggg gggatccgat ctcaatatga agttatgcat
      481 attactggcc gtcgtggcct ttgttggcct ctcgctcggg agtttggatt gtggattagc
      541 ttctaacgag tctccttaca acgaagcaaa tagcaatctg acgtacatat ccgatgctga
      601 tttcattcaa ggaggaaaaa ctggtaatgt ccaaaaagac ttgcttatga aactcaggaa
      661 gccatatacg gttttgagat actttccgga tggaatccga aattgttaca gtctaaatgt
      721 caagcaagac acaaattatc taatcagggt tatgtttcga tatggaaact atgacgggct
      781 taacaattct ccaagattcg acctctatct tggtcctaat atttggacaa ccatagatat
      841 ggggaagtca ggtgatggag ttctggagga gatcattcac atcacaaggt ctaacatctt
      901 ggacatatgt ctcgtcaaga cagggacgag tacaccaatg atatcatcca tagagttgag
      961 acccttgcta tatgatactt atattgctca aaccggttcc ttgaggaatt ataaccggtt
     1021 ctatttcacc gattcaaaca actatatacg ttatccccaa gatgtgcatg atagaatttg
     1081 ggtgccgttg atattaccag aatggacgca tataaacaca agtcaccacg ttattgattc
     1141 tatagatggc tatgacccac cacaagatgt acttaggacc ggcgccatgc ccgctaatgc
     1201 cagtgatcca atgaccatta cctggaattt gaagacggct actgaccagg tttacggtta
     1261 tatttacatc gccgagatca tggaagtcca ggccaatgaa actagagaat tcgaagttgt
     1321 ggtaaataat aaagttcatt tcgatccttt ccgtcctacc agatttgaag cacaagttat
     1381 gttcaacaat gtaccactga catgcgaggg aggattttgt cgtctgcaac ttataaaaac
     1441 tccgaaatcg actcttccac ccttgatgaa tgctttcgaa attttcacgg gcattgagtt
     1501 tcctcaatca gaaacaaatc aaaatgatgt cattgctgta aagaatattc aagctagtta
     1561 tggactgaac agaatcagct ggcagggcga tccatgtgtc cctaaacagt ttttgtggac
     1621 tggcttaagc tgcaacgtaa ttgatgtgtc cacaccacca agaatcgtga aattagattt
     1681 gtcttcaagt ggattaaatg gagttatacc acctagcata caaaatctaa ctcaacttca
     1741 agaactggac ctgtcccaaa acaacttgac tggtaaagtg cctgaatttc tagccaagat
     1801 gaaatatttg ttggtcataa acttaagtgg aaacaaactt agcggtttgg ttcctcaagc
     1861 ccttcttgat agaaaaaagg aaggattaaa gctattggtt gacgaaaata tgatttgtgt
     1921 gtcttgcggt acactggagg tgctgttcca gggaccaggg agcgacaaaa ctcacacatg
     1981 cccaccgtgc ccagcacctg aactcctggg gggaccgtca gtcttcctct tccccccaaa
     2041 acccaaggac accctcatga tctcccggac ccctgaggtc acatgcgtgg tggtggacgt
     2101 gagccacgaa gaccctgagg tcaagttcaa ctggtacgtg gacggcgtgg aggtgcataa
     2161 tgccaagaca aagccgcggg aggagcagta caacagcacg taccgtgtgg tcagcgtcct
     2221 caccgtcctg caccaggact ggctgaatgg caaggagtac aagtgcaagg tctccaacaa
     2281 agccctccca gcccccatcg agaaaaccat ctccaaagcc aaagggcagc cccgagaacc
     2341 acaggtgtac accctgcccc catcccggga ggagatgacc aagaaccagg tcagcctgac
     2401 ctgcctggtc aaaggcttct atcccagcga catcgccgtg gagtgggaga gcaatgggca
     2461 gccggagaac aactacaaga ccacgcctcc cgtgctggac tccgacggct ccttcttcct
     2521 ctacagcaag ctcaccgtgg acaagagcag gtggcagcag gggaacgtct tctcatgctc
     2581 cgtgatgcat gagggtctgc acaaccacta cacgcagaag agcctctccc tgtctccggg
     2641 taaatctaga gggcccttcg aaggtaagcc tatccctaac cctctcctcg gtctcgattc
     2701 tacgcgtacc ggtcatcatc accatcacca ttgagtttaa acccgctgat cagcctcgac
     2761 tgtgccttct aaggcctgag ctcgctgatc agcctcgatc gaggatccag acatgataag
     2821 atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg
     2881 tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa
     2941 caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta
     3001 aagcaagtaa aacctctaca aatgtggtat ggctgattat gatcagtcga cctgcaggca
     3061 tgcaagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     3121 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     3181 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     3241 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     3301 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     3361 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     3421 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     3481 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     3541 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     3601 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     3661 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     3721 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     3781 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     3841 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     3901 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     3961 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     4021 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     4081 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     4141 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     4201 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     4261 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     4321 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     4381 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     4441 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     4501 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     4561 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     4621 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     4681 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     4741 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     4801 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     4861 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     4921 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     4981 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     5041 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     5101 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     5161 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     5221 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag
     5281 gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca
     5341 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc
     5401 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc
     5461 agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag
     5521 gagaaaatac cgcatcaggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg
     5581 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg
     5641 attaagttgg gtaacgccag ggttttccca gtcacgacgt t
//
