LOCUS       sequence-296491-        4050 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector, with insert of the nucleocapsid (N)
            gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4050)
  AUTHORS   Kim DK, Knapp JJ, Kuang D, Chawla A, Cassonnet P, Lee H, 
            Sheykhkarimli D, Samavarchi-Tehrani P, Abdouni H, Rayhan A, Li R, 
            Pogoutse O, Coyaud E, van der Werf S, Demeret C, Gingras AC, Taipale
            M, Raught B, Jacob Y, Roth FP
  TITLE     A Comprehensive, Flexible Collection of SARS-CoV-2 Coding Regions.
  JOURNAL   G3 (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554.
  PUBMED    32763951
REFERENCE   2  (bases 1 to 4050)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554."
COMMENT     Sequence Label: pDONR223 SARS-CoV-2 N
FEATURES             Location/Qualifiers
     source          1..4050
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(79..106)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(198..284)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     333..355
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     347..364
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     348..364
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    380..479
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(668..679)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     protein_bind    complement(1740..1839)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1856..1875)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1857..1875)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1880..1896)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1880..1896)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2327..3118
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3211..3799
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3700..3719
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3953..3970
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat acgcgtaccg ctagccagga
       61 agagtttgta gaaacgcaaa aaggccatcc gtcaggatgg ccttctgctt agtttgatgc
      121 ctggcagttt atggcgggcg tcctgcccgc caccctccgg gccgttgctt cacaacgttc
      181 aaatccgctc ccggcggatt tgtcctactc aggagagcgt tcaccgacaa acaacagata
      241 aaacgaaagg cccagtcttc cgactgagcc tttcgtttta tttgatgcct ggcagttccc
      301 tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc
      361 cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga cctgttcgtt
      421 gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa aagttggcat
      481 gtccgacaac ggtccccaga accagcgtaa cgctccaagg atcaccttcg gtggacctag
      541 cgactccacc ggcagcaacc agaacggaga acgctccgga gccagaagca agcagcgtag
      601 gccacagggc ctgcctaaca acaccgccag ctggttcacc gctctgaccc agcacggaaa
      661 ggaagacctg aagttcccaa ggggacaggg cgtgccaatc aacaccaaca gctctccaga
      721 cgaccagatc ggatactaca ggcgtgccac caggcgcatc agaggaggcg acggaaagat
      781 gaaggacctg tctcctaggt ggtacttcta ctacctgggc accggaccag aggctggtct
      841 gccatacggc gccaacaagg acggcatcat ctgggtggct accgagggag ctctgaacac
      901 cccaaaggac cacatcggaa cccgtaaccc tgccaacaac gctgccatcg tgctgcagct
      961 gccacaggga accaccctgc ccaagggatt ctacgctgag ggctccaggg gtggaagcca
     1021 ggctagctct cgttcttcca gccgttctag gaacagctcc cgcaactcta ccccaggaag
     1081 ctccagaggc accagcccag ctcgtatggc cggcaacgga ggtgacgccg ctctggctct
     1141 gctgctgctg gaccgtctga accagctgga aagcaagatg tccggaaagg gtcagcagca
     1201 gcagggccag accgtgacca agaagtctgc cgctgaggcc tctaagaagc ccaggcagaa
     1261 gaggaccgct accaaggcct acaacgtgac ccaggccttc ggaagacgtg gacctgaaca
     1321 gacccaggga aacttcggcg accaggagct gatccgccag ggaaccgact acaagcactg
     1381 gccacagatc gctcagttcg ccccttctgc tagcgccttc ttcggaatgt ccaggatcgg
     1441 aatggaggtg accccttccg gcacctggct gacctacacc ggagctatca agctggacga
     1501 caaggacccc aacttcaagg accaggtgat cctgctgaac aagcacatcg acgcctacaa
     1561 gaccttccca cctaccgagc caaagaagga caagaagaag aaggccgacg aaacccaggc
     1621 cctgccacag aggcagaaga agcagcagac cgtgaccctg ctgccagctg ctgacctgga
     1681 cgacttctcc aagcagctgc agcagtctat gagctctgct gactccaccc aggcttaata
     1741 cccaactttc ttgtacaaag ttggcattat aagaaagcat tgcttatcaa tttgttgcaa
     1801 cgaacaggtc actatcagtc aaaataaaat cattatttgc catccagctg atatccccta
     1861 tagtgagtcg tattacatgg tcatagctgt ttcctggcag ctctggcccg tgtctcaaaa
     1921 tctctgatgt tacattgcac aagataaaaa tatatcatca tgcctcctct agaccagcca
     1981 ggacagaaat gcctcgactt cgctgctacc caaggttgcc gggtgacgca caccgtggaa
     2041 acggatgaag gcacgaaccc agtggacata agcctgttcg gttcgtaagc tgtaatgcaa
     2101 gtagcgtatg cgctcacgca actggtccag aaccttgacc gaacgcagcg gtggtaacgg
     2161 cgcagtggcg gttttcatgg cttgttatga ctgttttttt ggggtacagt ctatgcctcg
     2221 ggcatccaag cagcaagcgc gttacgccgt gggtcgatgt ttgatgttat ggagcagcaa
     2281 cgatgttacg cagcagggca gtcgccctaa aacaaagtta aacattatga gggaagcggt
     2341 gatcgccgaa gtatcgactc aactatcaga ggtagttggc gtcatcgagc gccatctcga
     2401 accgacgttg ctggccgtac atttgtacgg ctccgcagtg gatggcggcc tgaagccaca
     2461 cagtgatatt gatttgctgg ttacggtgac cgtaaggctt gatgaaacaa cgcggcgagc
     2521 tttgatcaac gaccttttgg aaacttcggc ttcccctgga gagagcgaga ttctccgcgc
     2581 tgtagaagtc accattgttg tgcacgacga catcattccg tggcgttatc cagctaagcg
     2641 cgaactgcaa tttggagaat ggcagcgcaa tgacattctt gcaggtatct tcgagccagc
     2701 cacgatcgac attgatctgg ctatcttgct gacaaaagca agagaacata gcgttgcctt
     2761 ggtaggtcca gcggcggagg aactctttga tccggttcct gaacaggatc tatttgaggc
     2821 gctaaatgaa accttaacgc tatggaactc gccgcccgac tgggctggcg atgagcgaaa
     2881 tgtagtgctt acgttgtccc gcatttggta cagcgcagta accggcaaaa tcgcgccgaa
     2941 ggatgtcgct gccgactggg caatggagcg cctgccggcc cagtatcagc ccgtcatact
     3001 tgaagctaga caggcttatc ttggacaaga agaagatcgc ttggcctcgc gcgcagatca
     3061 gttggaagaa tttgtccact acgtgaaagg cgagatcacc aaggtagtcg gcaaataacc
     3121 ctcgagccac ccatgaccaa aatcccttaa cgtgagttac gcgtcgttcc actgagcgtc
     3181 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     3241 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     3301 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     3361 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     3421 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     3481 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     3541 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     3601 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     3661 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     3721 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     3781 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     3841 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     3901 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     3961 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
     4021 gattcattaa tgcagctggc acgacaggtt
//
