LOCUS       sequence-296492-        6612 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector, with insert of the spike (S) gene's
            CDS from SARS-CoV-2 isolate Wuhan-Hu-1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6612)
  AUTHORS   Kim DK, Knapp JJ, Kuang D, Chawla A, Cassonnet P, Lee H, 
            Sheykhkarimli D, Samavarchi-Tehrani P, Abdouni H, Rayhan A, Li R, 
            Pogoutse O, Coyaud E, van der Werf S, Demeret C, Gingras AC, Taipale
            M, Raught B, Jacob Y, Roth FP
  TITLE     A Comprehensive, Flexible Collection of SARS-CoV-2 Coding Regions.
  JOURNAL   G3 (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554.
  PUBMED    32763951
REFERENCE   2  (bases 1 to 6612)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554."
COMMENT     Sequence Label: pDONR223 SARS-CoV-2 S
FEATURES             Location/Qualifiers
     source          1..6612
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(147..174)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(266..352)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     401..423
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     415..432
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     416..432
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    448..547
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(4370..4469)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(4486..4505)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4487..4505)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4510..4526)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4510..4526)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             4957..5748
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      5841..6429
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6330..6349
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6583..6600
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
       61 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatac gcgtaccgct
      121 agccaggaag agtttgtaga aacgcaaaaa ggccatccgt caggatggcc ttctgcttag
      181 tttgatgcct ggcagtttat ggcgggcgtc ctgcccgcca ccctccgggc cgttgcttca
      241 caacgttcaa atccgctccc ggcggatttg tcctactcag gagagcgttc accgacaaac
      301 aacagataaa acgaaaggcc cagtcttccg actgagcctt tcgttttatt tgatgcctgg
      361 cagttcccta ctctcgcgtt aacgctagca tggatgtttt cccagtcacg acgttgtaaa
      421 acgacggcca gtcttaagct cgggccccaa ataatgattt tattttgact gatagtgacc
      481 tgttcgttgc aacaaattga tgagcaatgc ttttttataa tgccaacttt gtacaaaaaa
      541 gttggcatgt tcgtgttcct ggtgctgctg ccactggtga gctcccagtg cgtgaacctg
      601 accaccagaa cccagctgcc acctgcctac accaactcct tcaccagggg cgtgtactac
      661 ccagacaagg tgttcagatc cagcgtgctg cactctaccc aggacctgtt cctgccattc
      721 ttctctaacg tgacctggtt ccacgccatc cacgtgtctg gaaccaacgg caccaagaga
      781 ttcgacaacc cagtgctgcc attcaacgac ggcgtgtact tcgcttctac cgagaagtct
      841 aacatcatca ggggttggat cttcggaacc accctggact ctaagaccca gtctctgctg
      901 atcgtgaaca acgctaccaa cgtggtgatc aaggtgtgcg agttccagtt ctgcaacgac
      961 ccattcctgg gagtgtacta ccacaagaac aacaagtctt ggatggaaag cgagttcagg
     1021 gtgtacagct ctgctaacaa ctgcaccttc gagtacgtgt ctcagccttt cctgatggac
     1081 ctggaaggaa agcagggaaa cttcaagaac ctgagagagt tcgtgttcaa gaacatcgac
     1141 ggttacttca agatatacag caagcacacc ccaatcaacc tggtgaggga cctgccacag
     1201 ggtttctctg ctctggagcc cctggtggac ctgccaatcg gcatcaacat caccaggttc
     1261 cagaccctgc tggctctgca caggtcttac ctgaccccag gcgacagcag ctctggatgg
     1321 accgccggcg ccgctgctta ctacgtggga tacctgcagc caaggacctt cctgctgaag
     1381 tacaacgaaa acggcaccat caccgacgcc gtggactgcg ctctggaccc actgtctgag
     1441 accaagtgca ccctgaagtc tttcaccgtg gaaaagggaa tctaccagac cagcaacttc
     1501 cgcgtgcagc ccaccgagtc tatcgtgaga ttcccaaaca tcaccaacct gtgcccattc
     1561 ggagaagtgt tcaacgctac caggttcgcc agcgtgtacg cttggaacag gaagagaatc
     1621 agcaactgcg tggccgacta ctctgtgctg tacaactctg cctccttctc caccttcaag
     1681 tgctacggcg tgtccccaac caagctgaac gacctgtgct tcaccaacgt gtacgctgac
     1741 tccttcgtga tcagaggaga cgaagtgaga cagatcgctc ccggtcagac cggaaagatc
     1801 gccgactaca actacaagct gccagacgac ttcaccggtt gcgtgatcgc ttggaactct
     1861 aacaacctgg actctaaggt gggaggcaac tacaactacc tgtacaggct gttccgcaag
     1921 agcaacctga agcctttcga aagggacatc agcaccgaaa tctaccaggc tggatctacc
     1981 ccatgcaacg gagtggaagg attcaactgc tacttccctc tgcagtctta cggattccag
     2041 ccaaccaacg gagtgggata ccagccatac agagtggtgg tgctgtcttt cgagctgctg
     2101 cacgctcctg ctaccgtgtg cggaccaaag aagtctacca acctggtgaa gaacaagtgc
     2161 gtgaacttca acttcaacgg actgaccgga accggagtgc tgaccgaatc caacaagaag
     2221 ttcctgcctt tccagcagtt cggtagagac atcgccgaca ccaccgacgc tgtgagagac
     2281 ccacagaccc tggaaatcct ggacatcacc ccctgctctt tcggaggagt gagcgtgatc
     2341 accccaggaa ccaacacctc caaccaggtg gccgtgctgt accaggacgt gaactgcacc
     2401 gaagtgccag tggccatcca cgctgaccag ctgaccccaa cctggagagt gtacagcacc
     2461 ggatctaacg tgttccagac ccgtgccggc tgcctgatcg gagccgagca cgtgaacaac
     2521 tcctacgaat gcgacatccc aatcggagct ggcatctgcg cttcttacca gacccagacc
     2581 aacagcccaa gaagggccag atccgtggcc tctcagagca tcatcgccta caccatgagc
     2641 ctgggcgctg aaaacagcgt ggcttacagc aacaactcta tcgccatccc aaccaacttc
     2701 accatctctg tgaccaccga aatcctgcca gtgagcatga ccaagaccag cgtggactgc
     2761 accatgtaca tctgcggaga ctctaccgaa tgctctaacc tgctgctgca gtacggcagc
     2821 ttctgcaccc agctgaaccg cgctctgacc ggaatcgctg tggagcagga caagaacacc
     2881 caggaagtgt tcgctcaggt gaagcagata tacaagaccc cacctatcaa ggacttcgga
     2941 ggattcaact tctctcagat cctgcccgac ccatctaagc cctctaagag gtctttcatc
     3001 gaagacctgc tgttcaacaa ggtgaccctg gccgacgccg gtttcatcaa gcagtacggt
     3061 gactgcctgg gagacatcgc cgctagagac ctgatctgcg cccagaagtt caacggtctg
     3121 accgtgctgc caccactgct gaccgacgag atgatcgccc agtacacctc tgccctgctg
     3181 gctggaacca tcacctctgg ttggaccttc ggagctggcg ccgccctgca gatcccattc
     3241 gctatgcaga tggcctaccg tttcaacggt atcggagtga cccagaacgt gctgtacgaa
     3301 aaccagaagc tgatcgctaa ccagttcaac tctgctatcg gaaagatcca ggactccctg
     3361 tcctctaccg cttctgccct gggaaagctg caggacgtgg tgaaccagaa cgcccaggcc
     3421 ctgaacaccc tggtgaagca gctgtcttct aacttcggag ccatctcctc tgtgctgaac
     3481 gacatcctgt ctaggctgga caaggtggaa gccgaggtgc agatcgacag actgatcacc
     3541 ggaagactgc agtctctgca gacctacgtg acccagcagc tgatcagggc cgctgaaatc
     3601 agagcttctg ccaacctggc tgccaccaag atgtccgaat gcgtgctggg ccagtctaag
     3661 agggtggact tctgcggaaa gggataccac ctgatgtcct tcccacagtc tgctccacac
     3721 ggagtggtgt tcctgcacgt gacctacgtg cccgcccagg aaaagaactt caccaccgcc
     3781 ccagctatct gccacgacgg aaaggctcac ttccccaggg agggagtgtt cgtgtctaac
     3841 ggaacccact ggttcgtgac ccagaggaac ttctacgagc cacagatcat caccaccgac
     3901 aacaccttcg tgtctggaaa ctgcgacgtg gtgatcggaa tcgtgaacaa caccgtgtac
     3961 gacccactgc agccagaact ggactctttc aaggaggaac tggacaagta cttcaagaac
     4021 cacacctctc cagacgtgga cctgggagac atctccggaa tcaacgcctc tgtggtgaac
     4081 atccagaagg aaatcgacag actgaacgag gtggccaaga acctgaacga atctctgatc
     4141 gacctgcagg aactgggaaa gtacgagcag tacatcaagt ggccctggta catctggctg
     4201 ggtttcatcg ctggtctgat cgctatcgtg atggtgacca tcatgctgtg ctgcatgacc
     4261 tcttgctgct cttgcctgaa gggatgctgc tcttgcggat cttgctgcaa gttcgacgag
     4321 gacgactccg agcctgtgct gaagggtgtg aagctgcact acacctaata cccaactttc
     4381 ttgtacaaag ttggcattat aagaaagcat tgcttatcaa tttgttgcaa cgaacaggtc
     4441 actatcagtc aaaataaaat cattatttgc catccagctg atatccccta tagtgagtcg
     4501 tattacatgg tcatagctgt ttcctggcag ctctggcccg tgtctcaaaa tctctgatgt
     4561 tacattgcac aagataaaaa tatatcatca tgcctcctct agaccagcca ggacagaaat
     4621 gcctcgactt cgctgctacc caaggttgcc gggtgacgca caccgtggaa acggatgaag
     4681 gcacgaaccc agtggacata agcctgttcg gttcgtaagc tgtaatgcaa gtagcgtatg
     4741 cgctcacgca actggtccag aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg
     4801 gttttcatgg cttgttatga ctgttttttt ggggtacagt ctatgcctcg ggcatccaag
     4861 cagcaagcgc gttacgccgt gggtcgatgt ttgatgttat ggagcagcaa cgatgttacg
     4921 cagcagggca gtcgccctaa aacaaagtta aacattatga gggaagcggt gatcgccgaa
     4981 gtatcgactc aactatcaga ggtagttggc gtcatcgagc gccatctcga accgacgttg
     5041 ctggccgtac atttgtacgg ctccgcagtg gatggcggcc tgaagccaca cagtgatatt
     5101 gatttgctgg ttacggtgac cgtaaggctt gatgaaacaa cgcggcgagc tttgatcaac
     5161 gaccttttgg aaacttcggc ttcccctgga gagagcgaga ttctccgcgc tgtagaagtc
     5221 accattgttg tgcacgacga catcattccg tggcgttatc cagctaagcg cgaactgcaa
     5281 tttggagaat ggcagcgcaa tgacattctt gcaggtatct tcgagccagc cacgatcgac
     5341 attgatctgg ctatcttgct gacaaaagca agagaacata gcgttgcctt ggtaggtcca
     5401 gcggcggagg aactctttga tccggttcct gaacaggatc tatttgaggc gctaaatgaa
     5461 accttaacgc tatggaactc gccgcccgac tgggctggcg atgagcgaaa tgtagtgctt
     5521 acgttgtccc gcatttggta cagcgcagta accggcaaaa tcgcgccgaa ggatgtcgct
     5581 gccgactggg caatggagcg cctgccggcc cagtatcagc ccgtcatact tgaagctaga
     5641 caggcttatc ttggacaaga agaagatcgc ttggcctcgc gcgcagatca gttggaagaa
     5701 tttgtccact acgtgaaagg cgagatcacc aaggtagtcg gcaaataacc ctcgagccac
     5761 ccatgaccaa aatcccttaa cgtgagttac gcgtcgttcc actgagcgtc agaccccgta
     5821 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     5881 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     5941 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     6001 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     6061 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     6121 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     6181 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     6241 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     6301 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     6361 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     6421 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     6481 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     6541 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     6601 gaagcggaag ag
//
