LOCUS       sequence-296494-        3272 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mouse synapsin-1 (mSyn1) promoter-mediates gene expression in 
            retinal neurons with fluorescent reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3272)
  AUTHORS   Wang Q, Zhuang PS, Huang H, Li L, Liu L, Webber HC, Dalal R, Siew L,
            Fligor CM, Chang KC, Nahmou M, Kreymerman A, Sun Y, Meyer JS, 
            Goldberg JL, Hu Y
  TITLE     Mouse gamma-Synuclein Promoter-Mediated Gene Expression and Editing 
            in Mammalian Retinal Ganglion Cells.
  JOURNAL   J Neurosci. 2020 Apr 9. pii: JNEUROSCI.0102-20.2020. doi: 
            10.1523/JNEUROSCI.0102-20.2020.
  PUBMED    32300046
REFERENCE   2  (bases 1 to 3272)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Neurosci.
            2020 Apr 9. pii: JNEUROSCI.0102-20.2020. doi: 
            10.1523/JNEUROSCI.0102-20.2020."
COMMENT     Sequence Label: pAM-AAV-mSyn1-EGFP
FEATURES             Location/Qualifiers
     source          1..3272
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     repeat_region   1..130
                     /label=AAV2 ITR
     CDS             join(1435..1437,1438..1440,1441..2154)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1435..1437,1438..1440,1441..2151)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1480..1501)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1741..1760)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2088..2109
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    2212..2800
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2265..2285)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2683..2694)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2827..2844)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2833..3057
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   3143..3272
                     /label=AAV2 ITR
ORIGIN
        1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct tgtagttaat gattaacccg ccatgctact tatttacgta gccatgctct
      181 aggtaccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      241 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      301 atcatatgtg agccttctta ttcagaccac caaaattact ttatttttcc acatgaaagt
      361 attatgtggc ttcctgtctg caaagaggaa gacatccatg aacactaatg acactgggtt
      421 tgggctatgt ccggagcaga ggaatgaggc catgtagact aaatatgtgc atgtggagga
      481 ggctgaaaac acatcagagc tagcgctgca ggaaatgctt ctgcattgca tacccagagt
      541 ttccttgctc atctgggagt ctgtgttttt cctagatgtg tgcacttgtg tgagattctc
      601 tgggtgtgag tcaaagtgtt atctgaatgt gtaatgtgtg ctcaatatgc tcatgtgtgt
      661 taccctgagc ttctgtgtct acatatatac ctggatgcct gtgtgttctg tgatgtacat
      721 atatattctg tctttccttc cttttctatt tgtgttattc catgtgttct ttcagattct
      781 caccaccaag ggcaaggata tgttaactac ccaagtgtcc acctccgcct gtctggtgat
      841 gtttacgcca cccccgtgct cttttctttg cccgacagag ttgttatagg agatgtctcc
      901 ccgggaacac tgcaggaagg agaatttcta catttatgtt cccctctgag tgtgcttcta
      961 tccccaaaat gccttcaaag gtgaaaatca acactggaaa cccaagtatc tgggaagggc
     1021 aagagtgtgt aagtgcaagt tagcctaagg aataggaaga ggttggtaaa cagggtagga
     1081 tcgtgggagg gagtttcgtt actacaggtc cggaccctca ggacaagaac cccaccccca
     1141 ctccccaaat tgcgcatccc ccgcccccat cagaggggga ggggaagagg ttgcggcgcg
     1201 gcgcatgcgc actgtcggat tcagcaccgc ggtcagagcc ttcgcctccg ctgccggcgc
     1261 gcaccaccac ctccccagca ccaaaggctg actgacgtca ctcactagcc ctccccaaac
     1321 tccccttcct cgccgccttg gtcgcgtcca tgctgccgtg agtccagtcg gaccgcacca
     1381 cgagaggtgc aagataggga ggtgcgggcg cgaccatacg ctctgcggga attcatggtg
     1441 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
     1501 gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag
     1561 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
     1621 accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac
     1681 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag
     1741 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
     1801 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
     1861 gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc
     1921 aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac
     1981 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
     2041 agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg
     2101 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtaaagatct
     2161 acgcgtggta cctctagagt cgacccgggc ggcctcgaga agcttatcga taatcaacct
     2221 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     2281 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     2341 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     2401 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     2461 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     2521 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     2581 gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt
     2641 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     2701 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     2761 cctcagacga gtcggatctc cctttgggcc gcctccccgc atcgataccg tcgactcgct
     2821 gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc
     2881 cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg
     2941 catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca
     3001 agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt
     3061 ctgaggcgga aagaaccagc tggggctcga ctagagcatg gctacgtaga taagtagcat
     3121 ggcgggttaa tcattaacta caaggaaccc ctagtgatgg agttggccac tccctctctg
     3181 cgcgctcgct cgctcactga ggccgggcga ccaaaggtcg cccgacgccc gggctttgcc
     3241 cgggcggcct cagtgagcga gcgagcgcgc ag
//
