LOCUS       sequence-296498-        3547 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mouse Isl2 (mIsl2) promoter-mediates gene expression in retinal 
            neurons with fluorescent reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3547)
  AUTHORS   Wang Q, Zhuang PS, Huang H, Li L, Liu L, Webber HC, Dalal R, Siew L,
            Fligor CM, Chang KC, Nahmou M, Kreymerman A, Sun Y, Meyer JS, 
            Goldberg JL, Hu Y
  TITLE     Mouse gamma-Synuclein Promoter-Mediated Gene Expression and Editing 
            in Mammalian Retinal Ganglion Cells.
  JOURNAL   J Neurosci. 2020 Apr 9. pii: JNEUROSCI.0102-20.2020. doi: 
            10.1523/JNEUROSCI.0102-20.2020.
  PUBMED    32300046
REFERENCE   2  (bases 1 to 3547)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Neurosci.
            2020 Apr 9. pii: JNEUROSCI.0102-20.2020. doi: 
            10.1523/JNEUROSCI.0102-20.2020."
COMMENT     Sequence Label: pAM-AAV-mIsl2-EGPF
FEATURES             Location/Qualifiers
     source          1..3547
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     repeat_region   1..130
                     /label=AAV2 ITR
     CDS             join(1710..1712,1713..1715,1716..2429)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1710..1712,1713..1715,1716..2426)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1755..1776)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2016..2035)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2363..2384
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    2487..3075
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2540..2560)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2958..2969)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3102..3119)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3108..3332
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   3418..3547
                     /label=AAV2 ITR
ORIGIN
        1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct tgtagttaat gattaacccg ccatgctact tatttacgta gccatgctct
      181 aggtaccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      241 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      301 atcatatgga ctgcaaccat ttcccagccc agcagcttcg gctcttagca gttggaccgc
      361 cgcgcctgaa ttagatagat ctcgggcagc gaggcctgcg ggatccaagt ccggacatag
      421 cttttggtcg agatacttta tcccagaagc ccacccgcgc cccacttaga gaggtatcaa
      481 gtttttggtg tcctttcaga atgggagtga gggaccaaca gaccaccgtt ggataaacac
      541 tgtttgtacc tcatcgattt gtctttcaac cctttgaaac aaaattcaca acgatttttg
      601 tttaaaaaaa tcccgagtct tagaaatcgc agatttttct aggattaaaa agccaccggg
      661 aagacggtaa attaccccgt gtcccacatt tgtttctgat tagttgtgtt gtaattggtt
      721 tgtgtaaccc aaaatcagag agttcgtaaa aatcttttac tgtatgagtg acacatcaca
      781 gcactgtttt taggctctat gcagctgaga ggagaaagga gagggtggga gtcagcatca
      841 atttaaccaa aagagcaaaa agtaggaggg aggaaaagtg cctaggccag ggtgggtggg
      901 aggactgtgg ctctaagtct ctggattcct tttggcttac ccacctgcaa catcgagttt
      961 tctacaggcg gcgcgggtgg gaaagtccgc atagagttta tccaaaccca ggtgctcatt
     1021 tcctacccgt tctccctaac ttggaccccg ccgaacaaag gctcaggccc accagggaga
     1081 aaggtggccg gctggaccag tgatggtccc gaaacagcga atcctctttt ctccctcaag
     1141 gtctgtcgtc cccaggagga atttttaatt taaaaattat aaaagaaaaa cacttttaag
     1201 ggggagacga ctctcatcta ttcccagaag actattgcct tgctaggcaa acgccctaca
     1261 agtccgaaga aagcgcggcg ctgcggcaga gcccgcgggg tggagatgcg gcttccaagg
     1321 gattgcggga cccggggctg cagagaggat ctaggaaggt ttcgggggag ggggctgcgc
     1381 tccattgttc actccgaacc aatcagagct ttcccacttc ctcccagcca atctccctgc
     1441 gcctccgtgt ctaccccagc ccaccctccg ggtccccatc atctcccgag caagccccag
     1501 ggcggtggga gagctgggtg acgctacagg tcccgcctcc agctcgggcc ccgggcgagt
     1561 gcgtgctgac gtcatgctgc gtgcgggccg gtgcggaatc gctccttcaa ctccgcgggg
     1621 caggagttag ttagcaaaga gcagaggctg ggcgctcgac cctggtcctt ctgtccccgg
     1681 ccgcaagctt tgctcacatc tctgaattca tggtgagcaa gggcgaggag ctgttcaccg
     1741 gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt
     1801 ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca
     1861 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt
     1921 gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg
     1981 aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg
     2041 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact
     2101 tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg
     2161 tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca
     2221 acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg
     2281 acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag
     2341 accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca
     2401 ctctcggcat ggacgagctg tacaagtaaa gatctacgcg tggtacctct agagtcgacc
     2461 cgggcggcct cgagaagctt atcgataatc aacctctgga ttacaaaatt tgtgaaagat
     2521 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
     2581 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
     2641 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca
     2701 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt
     2761 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg
     2821 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga
     2881 aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt
     2941 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc
     3001 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt
     3061 gggccgcctc cccgcatcga taccgtcgac tcgctgatca gcctcgactg tgccttctag
     3121 ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac
     3181 tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca
     3241 ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag
     3301 caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg
     3361 ctcgactaga gcatggctac gtagataagt agcatggcgg gttaatcatt aactacaagg
     3421 aacccctagt gatggagttg gccactccct ctctgcgcgc tcgctcgctc actgaggccg
     3481 ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag
     3541 cgcgcag
//
