LOCUS       sequence-296507-       16708 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Inducing plant cells to enter mitosis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16708)
  AUTHORS   Xu J, Lee YJ, Liu B
  TITLE     Establishment of a mitotic model system by transient expression of 
            the D-type cyclin in differentiated leaf cells of tobacco (Nicotiana
            benthamiana).
  JOURNAL   New Phytol. 2019 Nov 2. doi: 10.1111/nph.16309.
  PUBMED    31679162
REFERENCE   2  (bases 1 to 16708)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "New Phytol.
            2019 Nov 2. doi: 10.1111/nph.16309."
COMMENT     Sequence Label: pGWB2-CYCD3;1
FEATURES             Location/Qualifiers
     source          1..16708
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(145..516)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            549..658
                     /note="incP origin of transfer"
     primer_bind     881..900
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1134..1151
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(1339..1989)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    2454..2478
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2602..2781
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        2634..2817
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             2838..3632
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2892..2911)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3502..3521
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      4022..4274
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    4823..4844
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4859..4864,4865..4882,4883..4889)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4897..4913
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4902..4924
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4921..4937
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4921..4937
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        5461..5806
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    5828..5852
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6984..7008)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      7040..7292
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      complement(7721..7973)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(8441..9466)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(8441..9466)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(9476..9820)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(9938..9955)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9938..9954)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9947..9969)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(10094..10113)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10304..10325
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    10572..10596
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(12192..13340)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(13639..14433)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  14625..15392
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      join(16085..16708,1..8)
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 tgagcgtcgg aaaggcgctc ggtcttgcct tgctcgtcgg tgatgtactt caccagctcc
       61 gcgaagtcgc tcttcttgat ggagcgcatg gggacgtgct tggcaatcac gcgcaccccc
      121 cggccgtttt agcggctaaa aaagtcatgg ctctgccctc gggcggacca cgcccatcat
      181 gaccttgcca agctcgtcct gcttctcttc gatcttcgcc agcagggcga ggatcgtggc
      241 atcaccgaac cgcgccgtgc gcgggtcgtc ggtgagccag agtttcagca ggccgcccag
      301 gcggcccagg tcgccattga tgcgggccag ctcgcggacg tgctcatagt ccacgacgcc
      361 cgtgattttg tagccctggc cgacggccag caggtaggcc gacaggctca tgccggccgc
      421 cgccgccttt tcctcaatcg ctcttcgttc gtctggaagg cagtacacct tgataggtgg
      481 gctgcccttc ctggttggct tggtttcatc agccatccgc ttgccctcat ctgttacgcc
      541 ggcggtagcc ggccagcctc gcagagcagg attcccgttg agcaccgcca ggtgcgaata
      601 agggacagtg aagaaggaac acccgctcgc gggtgggcct acttcaccta tcctgcccgg
      661 ctgacgccgt tggatacacc aaggaaagtc tacacgaacc ctttggcaaa atcctgtata
      721 tcgtgcgaaa aaggatggat ataccgaaaa aatcgctata atgaccccga agcagggtta
      781 tgcagcggaa aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
      841 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
      901 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
      961 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1021 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1081 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1141 cagtgagcga ggaagcggaa gagcgccaga aggccgccag agaggccgag cgcggccgtg
     1201 aggcttggac gctagggcag ggcatgaaaa agcccgtagc gggctgctac gggcgtctga
     1261 cgcggtggaa agggggaggg gatgttgtct acatggctct gctgtagtga gtgggttgcg
     1321 ctccggcagc ggtcctgatc aatcgtcacc ctttctcggt ccttcaacgt tcctgacaac
     1381 gagcctcctt ttcgccaatc catcgacaat caccgcgagt ccctgctcga acgctgcgtc
     1441 cggaccggct tcgtcgaagg cgtctatcgc ggcccgcaac agcggcgaga gcggagcctg
     1501 ttcaacggtg ccgccgcgct cgccggcatc gctgtcgccg gcctgctcct caagcacggc
     1561 cccaacagtg aagtagctga ttgtcatcag cgcattgacg gcgtccccgg ccgaaaaacc
     1621 cgcctcgcag aggaagcgaa gctgcgcgtc ggccgtttcc atctgcggtg cgcccggtcg
     1681 cgtgccggca tggatgcgcg cgccatcgcg gtaggcgagc agcgcctgcc tgaagctgcg
     1741 ggcattcccg atcagaaatg agcgccagtc gtcgtcggct ctcggcaccg aatgcgtatg
     1801 attctccgcc agcatggctt cggccagtgc gtcgagcagc gcccgcttgt tcctgaagtg
     1861 ccagtaaagc gccggctgct gaacccccaa ccgttccgcc agtttgcgtg tcgtcagacc
     1921 gtctacgccg acctcgttca acaggtccag ggcggcacgg atcactgtat tcggctgcaa
     1981 ctttgtcatg cttgacactt tatcactgat aaacataata tgtccaccaa cttatcagtg
     2041 ataaagaatc cgcgcgttca atcggaccag cggaggctgg tccggaggcc agacgtgaaa
     2101 cccaacatac ccctgatcgt aattctgagc actgtcgcgc tcgacgctgt cggcatcggc
     2161 ctgattatgc cggtgctgcc gggcctcctg cgcgatctgg ttcactcgaa cgacgtcacc
     2221 gcccactatg gcattctgct ggcgctgtat gcgttggtgc aatttgcctg cgcacctgtg
     2281 ctgggcgcgc tgtcggatcg tttcgggcgg cggccaatct tgctcgtctc gctggccggc
     2341 gccagatctg gggaaccctg tggttggcat gcacatacaa atggacgaac ggataaacct
     2401 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
     2461 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
     2521 tcatgagcgg agaattaagg gagtcacgtt atgacccccg ccgatgacgc gggacaagcc
     2581 gttttacgtt tggaactgac agaaccgcaa cgttgaagga gccactcagc cgcgggtttc
     2641 tggagtttaa tgagctaagc acatacgtca gaaaccatta ttgcgcgttc aaaagtcgcc
     2701 taaggtcact atcagctagc aaatatttct tgtcaaaaat gctccactga cgttccataa
     2761 attcccctcg gtatccaatt agagtctcat attcactctc aatccaaata atctgcaccg
     2821 gatctggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt
     2881 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
     2941 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
     3001 gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg
     3061 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
     3121 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca
     3181 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc
     3241 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
     3301 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca
     3361 aggcgcgcat gcccgacggc gatgatctcg tcgtgaccca tggcgatgcc tgcttgccga
     3421 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg
     3481 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg
     3541 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg
     3601 ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga
     3661 ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag
     3721 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
     3781 catgctggag ttcttcgccc acgggatctc tgcggaacag gcggtcgaag gtgccgatat
     3841 cattacgaca gcaacggccg acaagcacaa cgccacgatc ctgagcgaca atatgatcgg
     3901 gcccggcgtc cacatcaacg gcgtcggcgg cgactgccca ggcaagaccg agatgcaccg
     3961 cgatatcttg ctgcgttcgg atattttcgt ggagttcccg ccacagaccc ggatgatccc
     4021 cgatcgttca aacatttggc aataaagttt cttaagattg aatcctgttg ccggtcttgc
     4081 gatgattatc atataatttc tgttgaatta cgttaagcat gtaataatta acatgtaatg
     4141 catgacgtta tttatgagat gggtttttat gattagagtc ccgcaattat acatttaata
     4201 cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc
     4261 tatgttacta gatcgggcct cctgtcaatg ctggcggcgg ctctggtggt ggttctggtg
     4321 gcggctctga gggtggtggc tctgagggtg gcggttctga gggtggcggc tctgagggag
     4381 gcggttccgg tggtggctct ggttccggtg attttgatta tgaaaagatg gcaaacgcta
     4441 ataagggggc tatgaccgaa aatgccgatg aaaacgcgct acagtctgac gctaaaggca
     4501 aacttgattc tgtcgctact gattacggtg ctgctatcga tggtttcatt ggtgacgttt
     4561 ccggccttgc taatggtaat ggtgctactg gtgattttgc tggctctaat tcccaaatgg
     4621 ctcaagtcgg tgacggtgat aattcacctt taatgaataa tttccgtcaa tatttacctt
     4681 ccctccctca atcggttgaa tgtcgccctt ttgtctttgg cccaatacgc aaaccgcctc
     4741 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     4801 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
     4861 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
     4921 caggaaacag ctatgaccat gattacgcca agcttgcatg cctgcaggtc cccagattag
     4981 ccttttcaat ttcagaaaga atgctaaccc acagatggtt agagaggctt acgcagcagg
     5041 tctcatcaag acgatctacc cgagcaataa tctccaggaa atcaaatacc ttcccaagaa
     5101 ggttaaagat gcagtcaaaa gattcaggac taactgcatc aagaacacag agaaagatat
     5161 atttctcaag atcagaagta ctattccagt atggacgatt caaggcttgc ttcacaaacc
     5221 aaggcaagta atagagattg gagtctctaa aaaggtagtt cccactgaat caaaggccat
     5281 ggagtcaaag attcaaatag aggacctaac agaactcgcc gtaaagactg gcgaacagtt
     5341 catacagagt ctcttacgac tcaatgacaa gaagaaaatc ttcgtcaaca tggtggagca
     5401 cgacacactt gtctactcca aaaatatcaa agatacagtc tcagaagacc aaagggcaat
     5461 tgagactttt caacaaaggg taatatccgg aaacctcctc ggattccatt gcccagctat
     5521 ctgtcacttt attgtgaaga tagtggaaaa ggaaggtggc tcctacaaat gccatcattg
     5581 cgataaagga aaggccatcg ttgaagatgc ctctgccgac agtggtccca aagatggacc
     5641 cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt
     5701 ggattgatgt gatatctcca ctgacgtaag ggatgacgca caatcccact atccttcgca
     5761 agacccttcc tctatataag gaagttcatt tcatttggag agaacacggg ggactctaga
     5821 gttatcaaca agtttgtaca aaaaagcagg cttaatggcg attcggaagg aggaagaaag
     5881 tagagaagaa cagagcaatt cgtttcttct tgatgctctc tactgcgaag aagagaaatg
     5941 ggacgatgaa ggagaagaag ttgaagaaaa ctcttccttg tcttcttctt cttctccatt
     6001 cgttgttttg caacaagatt tgttctggga agatgaagat ctggttacac tcttctccaa
     6061 agaagaagaa caaggactca gctgtctcga tgatgtttat ctttccacgg atcgaaaaga
     6121 agctgttggt tggattctga gagtcaacgc tcattatggc ttctctactt tagcagctgt
     6181 tttagccata acttatctcg ataagttcat ctgtagctac agcttacaga gagacaaacc
     6241 atggatgctt cagctcgttt ctgtcgcgtg tctctcatta gctgctaaag tcgaagaaac
     6301 ccaagtccct cttcttctag actttcaagt ggaggagaca aagtatgtgt ttgaagcaaa
     6361 aaccatacag agaatggagc tactgattct gtctactctc gagtggaaga tgcatctcat
     6421 tactccaatt tcgttcgtag accacattat caggagattg ggacttaaga acaatgctca
     6481 ctgggatttc ctcaacaaat gccaccgtct cctcctctct gtaatctccg attcaagatt
     6541 tgtcgggtac ctcccatcag tagttgccgc agctaccatg atgcgaatta tagagcaagt
     6601 tgatcccttt gaccctcttt cataccaaac taatctcctc ggtgtcctta acttaaccaa
     6661 ggaaaaggtg aaaacttgct acgatctaat cctccaacta ccagtggacc gcatcggttt
     6721 acagatccaa atccaatctt ccaagaaacg caagagtcac gattcatcat catcgttgaa
     6781 cagtccaagc tgcgtgattg atgcaaaccc tttcaatagc gacgaaagct caaacgattc
     6841 gtggtcagcg agttcgtgca acccaccaac gtcgtcgtcg tccccgcagc aacaacctcc
     6901 attgaagaag atgagaggag ctgaagagaa tgagaagaag aagccgattt tgcatctgcc
     6961 atgggcaatc gtagccactc caaacccagc tttcttgtac aaagtggttg ataacagcgc
     7021 ttagagctcg aatttccccg atcgttcaaa catttggcaa taaagtttct taagattgaa
     7081 tcctgttgcc ggtcttgcga tgattatcat ataatttctg ttgaattacg ttaagcatgt
     7141 aataattaac atgtaatgca tgacgttatt tatgagatgg gtttttatga ttagagtccc
     7201 gcaattatac atttaatacg cgatagaaaa caaaatatag cgcgcaaact aggataaatt
     7261 atcgcgcgcg gtgtcatcta tgttactaga tcgggaatta gcttcatcaa cgcaagacat
     7321 gcgcacgacc gtctgacagg agaggaattt ccgacgagca cagaaaggac ttgctcttgg
     7381 acgtaggcct atttctcagg cacatgtatc aagtgttcgg acgtgggttt tcgatggtgt
     7441 atcagccgcc gccaactggg agatgaggag gctttcttgg ggggcagtca gcagttcatt
     7501 tcacaagaca gaggaacttg taaggagatg cactgattta tcttggcgca aaccagcagg
     7561 acgaattagt gggaatagcc cgcgaatatc taagttatgc ctgtcggcat gagcagaaac
     7621 ttccaattcg aaacagtttg gagaggttgt ttttgggcat accttttgtt agtcagcctc
     7681 tcgattgctc atcgtcatta cacagtaccg aagtttgatc gatctagtaa catagatgac
     7741 accgcgcgcg ataatttatc ctagtttgcg cgctatattt tgttttctat cgcgtattaa
     7801 atgtataatt gcgggactct aatcataaaa acccatctca taaataacgt catgcattac
     7861 atgttaatta ttacatgctt aacgtaattc aacagaaatt atatgataat catcgcaaga
     7921 ccggcaacag gattcaatct taagaaactt tattgccaaa tgtttgaacg atctgcttcg
     7981 acgcactcct tctttactcc accatctcgt ccttattgaa aacgtgggta gcaccaaaac
     8041 gaatcaagtc gctggaactg aagttaccaa tcacgctgga tgatttgcca gttggattaa
     8101 tcttgccttt ccccgcatga ataatattga tgaatgcatg cgtgaggggt atttcgattt
     8161 tggcaatagc tgcaattgcc gcgacatcct ccaacgagca taattcttca gaaaaatagc
     8221 gatgttccat gttgtcaggg catgcatgat gcacgttatg aggtgacggt gctaggcagt
     8281 attccctcaa agtttcatag tcagtatcat attcatcatt gcattcctgc aagagagaat
     8341 tgagacgcaa tccacacgct gcggcaacct tccggcgttc gtggtctatt tgctcttgga
     8401 cgttgcaaac gtaagtgttg gatcccggtc ggcatctact ctattccttt gccctcggac
     8461 gagtgctggg gcgtcggttt ccactatcgg cgagtacttc tacacagcca tcggtccaga
     8521 cggccgcgct tctgcgggcg atttgtgtac gcccgacagt cccggctccg gatcggacga
     8581 ttgcgtcgca tcgaccctgc gcccaagctg catcatcgaa attgccgtca accaagctct
     8641 gatagagttg gtcaagacca atgcggagca tatacgcccg gagccgcggc gatcctgcaa
     8701 gctccggatg cctccgctcg aagtagcgcg tctgctgctc catacaagcc aaccacggcc
     8761 tccagaagaa gatgttggcg acctcgtatt gggaatcccc gaacatcgcc tcgctccagt
     8821 caatgaccgc tgttatgcgg ccattgtccg tcaggacatt gttggagccg aaatccgcgt
     8881 gcacgaggtg ccggacttcg gggcagtcct cggcccaaag catcagctca tcgagagcct
     8941 gcgcgacgga cgcactgacg gtgtcgtcca tcacagtttg ccagtgatac acatggggat
     9001 cagcaatcgc gcatatgaaa tcacgccatg tagtgtattg accgattcct tgcggtccga
     9061 atgggccgaa cccgctcgtc tggctaagat cggccgcagc gatcgcatcc atggcctccg
     9121 cgaccggctg cagaacagcg ggcagttcgg tttcaggcag gtcttgcaac gtgacaccct
     9181 gtgcacggcg ggagatgcaa taggtcaggc tctcgctgaa ttccccaatg tcaagcactt
     9241 ccggaatcgg gagcgcggcc gatgcaaagt gccgataaac ataacgatct ttgtagaaac
     9301 catcggcgca gctatttacc cgcaggacat atccacgccc tcctacatcg aagctgaaag
     9361 cacgagattc ttcgccctcc gagagctgca tcaggtcgga gacgctgtcg aacttttcga
     9421 tcagaaactt ctcgacagac gtcgcggtga gttcaggctt tttcatatcg gggtcgtcct
     9481 ctccaaatga aatgaacttc cttatataga ggaagggtct tgcgaaggat agtgggattg
     9541 tgcgtcatcc cttacgtcag tggagatatc acatcaatcc acttgctttg aagacgtggt
     9601 tggaacgtct tctttttcca cgatgctcct cgtgggtggg ggtccatctt tgggaccact
     9661 gtcggcagag gcatcttgaa cgatagcctt tcctttatcg caatgatggc atttgtaggt
     9721 gccaccttcc ttttctactg tccttttgat gaagtgacag atagctgggc aatggaatcc
     9781 gaggaggttt cccgatatta ccctttgttg aaaagtctca atagcccttt ggtcttctga
     9841 gactgtatct ttgatattct tggagtagac gagagtgtcg tgctccacca tgttgacgga
     9901 tctctaggac gcgtcgacgc gtcctagaag ctaattcact ggccgtcgtt ttacaacgtc
     9961 gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg
    10021 ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc
    10081 tgaatggcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
    10141 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
    10201 ctcgacccca aaaaacttga tttgggtgat ggttcacgta gtgggccatc gccctgatag
    10261 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
    10321 actggaacaa cactcaaccc tatctcgggc tattcttttg atttataagg gattttgccg
    10381 atttcggaac caccatcaaa caggattttc gcctgctggg gcaaaccagc gtggaccgct
    10441 tgctgcaact ctctcagggc caggcggtga agggcaatca gctgttgccc gtctcactgg
    10501 tgaaaagaaa aaccacccca gtacattaaa aacgtccgca atgtgttatt aagttgtcta
    10561 agcgtcaatt tgtttacacc acaatatatc ctgccaccag ccagccaaca gctccccgac
    10621 cggcagctcg gcacaaaatc accactcgat acaggcagcc catcagtccg ggacggcgtc
    10681 agcgggagag ccgttgtaag gcggcagact ttgctcatgt taccgatgct attcggaaga
    10741 acggcaacta agctgccggg tttgaaacac ggatgatctc gcggagggta gcatgttgat
    10801 tgtaacgatg acagagcgtt gctgcctgtg atcaaatatc atctccctcg cagagatccg
    10861 aattatcagc cttcttattc atttctcgct taaccgtgac aggctgtcga tcttgagaac
    10921 tatgccgaca taataggaaa tcgctggata aagccgctga ggaagctgag tggcgctatt
    10981 tctttagaag tgaacgttga cgatatcaac tcccctatcc attgctcacc gaatggtaca
    11041 ggtcggggac ccgaagttcc gactgtcggc ctgatgcatc cccggctgat cgaccccaga
    11101 tctggggctg agaaagccca gtaaggaaac aactgtaggt tcgagtcgcg agatcccccg
    11161 gaaccaaagg aagtaggtta aacccgctcc gatcaggccg agccacgcca ggccgagaac
    11221 attggttcct gtaggcatcg ggattggcgg atcaaacact aaagctactg gaacgagcag
    11281 aagtcctccg gccgccagtt gccaggcggt aaaggtgagc agaggcacgg gaggttgcca
    11341 cttgcgggtc agcacggttc cgaacgccat ggaaaccgcc cccgccaggc ccgctgcgac
    11401 gccgacagga tctagcgctg cgtttggtgt caacaccaac agcgccacgc ccgcagttcc
    11461 gcaaatagcc cccaggaccg ccatcaatcg tatcgggcta cctagcagag cggcagagat
    11521 gaacacgacc atcagcggct gcacagcgcc taccgtcgcc gcgaccccgc ccggcaggcg
    11581 gtagaccgaa ataaacaaca agctccagaa tagcgaaata ttaagtgcgc cgaggatgaa
    11641 gatgcgcatc caccagattc ccgttggaat ctgtcggacg atcatcacga gcaataaacc
    11701 cgccggcaac gcccgcagca gcataccggc gacccctcgg cctcgctgtt cgggctccac
    11761 gaaaacgccg gacagatgcg ccttgtgagc gtccttgggg ccgtcctcct gtttgaagac
    11821 cgacagccca atgatctcgc cgtcgatgta ggcgccgaat gccacggcat ctcgcaaccg
    11881 ttcagcgaac gcctccatgg gctttttctc ctcgtgctcg taaacggacc cgaacatctc
    11941 tggagctttc ttcagggccg acaatcggat ctcgcggaaa tcctgcacgt cggccgctcc
    12001 aagccgtcga atctgagcct taatcacaat tgtcaatttt aatcctctgt ttatcggcag
    12061 ttcgtagagc gcgccgtgcg tcccgagcga tactgagcga agcaagtgcg tcgagcagtg
    12121 cccgcttgtt cctgaaatgc cagtaaagcg ctggctgctg aacccccagc cggaactgac
    12181 cccacaaggc cctagcgttt gcaatgcacc aggtcatcat tgacccaggc gtgttccacc
    12241 aggccgctgc ctcgcaactc ttcgcaggct tcgccgacct gctcgcgcca cttcttcacg
    12301 cgggtggaat ccgatccgca catgaggcgg aaggtttcca gcttgagcgg gtacggctcc
    12361 cggtgcgagc tgaaatagtc gaacatccgt cgggccgtcg gcgacagctt gcggtacttc
    12421 tcccatatga atttcgtgta gtggtcgcca gcaaacagca cgacgatttc ctcgtcgatc
    12481 aggacctggc aacgggacgt tttcttgcca cggtccagga cgcggaagcg gtgcagcagc
    12541 gacaccgatt ccaggtgccc aacgcggtcg gacgtgaagc ccatcgccgt cgcctgtagg
    12601 cgcgacaggc attcctcggc cttcgtgtaa taccggccat tgatcgacca gcccaggtcc
    12661 tggcaaagct cgtagaacgt gaaggtgatc ggctcgccga taggggtgcg cttcgcgtac
    12721 tccaacacct gctgccacac cagttcgtca tcgtcggccc gcagctcgac gccggtgtag
    12781 gtgatcttca cgtccttgtt gacgtggaaa atgaccttgt tttgcagcgc ctcgcgcggg
    12841 attttcttgt tgcgcgtggt gaacagggca gagcgggccg tgtcgtttgg catcgctcgc
    12901 atcgtgtccg gccacggcgc aatatcgaac aaggaaagct gcatttcctt gatctgctgc
    12961 ttcgtgtgtt tcagcaacgc ggcctgcttg gcctcgctga cctgttttgc caggtcctcg
    13021 ccggcggttt ttcgcttctt ggtcgtcata gttcctcgcg tgtcgatggt catcgacttc
    13081 gccaaacctg ccgcctcctg ttcgagacga cgcgaacgct ccacggcggc cgatggcgcg
    13141 ggcagggcag ggggagccag ttgcacgctg tcgcgctcga tcttggccgt agcttgctgg
    13201 accatcgagc cgacggactg gaaggtttcg cggggcgcac gcatgacggt gcggcttgcg
    13261 atggtttcgg catcctcggc ggaaaacccc gcgtcgatca gttcttgcct gtatgccttc
    13321 cggtcaaacg tccgattcat tcaccctcct tgcgggattg ccccgactca cgccggggca
    13381 atgtgccctt attcctgatt tgacccgcct ggtgccttgg tgtccagata atccacctta
    13441 tcggcaatga agtcggtccc gtagaccgtc tggccgtcct tctcgtactt ggtattccga
    13501 atcttgccct gcacgaatac cagcgacccc ttgcccaaat acttgccgtg ggcctcggcc
    13561 tgagagccaa aacacttgat gcggaagaag tcggtgcgct cctgcttgtc gccggcatcg
    13621 ttgcgccaca tctaggtact aaaacaattc atccagtaaa atataatatt ttattttctc
    13681 ccaatcaggc ttgatcccca gtaagtcaaa aaatagctcg acatactgtt cttccccgat
    13741 atcctccctg atcgaccgga cgcagaaggc aatgtcatac cacttgtccg ccctgccgct
    13801 tctcccaaga tcaataaagc cacttacttt gccatctttc acaaagatgt tgctgtctcc
    13861 caggtcgccg tgggaaaaga caagttcctc ttcgggcttt tccgtcttta aaaaatcata
    13921 cagctcgcgc ggatctttaa atggagtgtc ttcttcccag ttttcgcaat ccacatcggc
    13981 cagatcgtta ttcagtaagt aatccaattc ggctaagcgg ctgtctaagc tattcgtata
    14041 gggacaatcc gatatgtcga tggagtgaaa gagcctgatg cactccgcat acagctcgat
    14101 aatcttttca gggctttgtt catcttcata ctcttccgag caaaggacgc catcggcctc
    14161 actcatgagc agattgctcc agccatcatg ccgttcaaag tgcaggacct ttggaacagg
    14221 cagctttcct tccagccata gcatcatgtc cttttcccgt tccacatcat aggtggtccc
    14281 tttataccgg ctgtccgtca tttttaaata taggttttca ttttctccca ccagcttata
    14341 taccttagca ggagacattc cttccgtatc ttttacgcag cggtattttt cgatcagttt
    14401 tttcaattcc ggtgatattc tcattttagc catttattat ttccttcctc ttttctacag
    14461 tatttaaaga taccccaaga agctaattat aacaagacga actccaattc actgttcctt
    14521 gcattctaaa accttaaata ccagaaaaca gctttttcaa agttgttttc aaagttggcg
    14581 tataacatag tatcgacgga gccgattttg aaaccacaat tatgggtgat gctgccaact
    14641 tactgattta gtgtatgatg gtgtttttga ggtgctccag tggcttctgt ttctatcagc
    14701 tgtccctcct gttcagctac tgacggggtg gtgcgtaacg gcaaaagcac cgccggacat
    14761 cagcgctatc tctgctctca ctgccgtaaa acatggcaac tgcagttcac ttacaccgct
    14821 tctcaacccg gtacgcacca gaaaatcatt gatatggcca tgaatggcgt tggatgccgg
    14881 gcaacagccc gcattatggg cgttggcctc aacacgattt tacgtcactt aaaaaactca
    14941 ggccgcagtc ggtaacctcg cgcatacagc cgggcagtga cgtcatcgtc tgcgcggaaa
    15001 tggacgaaca gtggggctat gtcggggcta aatcgcgcca gcgctggctg ttttacgcgt
    15061 atgacagtct ccggaagacg gttgttgcgc acgtattcgg tgaacgcact atggcgacgc
    15121 tggggcgtct tatgagcctg ctgtcaccct ttgacgtggt gatatggatg acggatggct
    15181 ggccgctgta tgaatcccgc ctgaagggaa agctgcacgt aatcagcaag cgatatacgc
    15241 agcgaattga gcggcataac ctgaatctga ggcagcacct ggcacggctg ggacggaagt
    15301 cgctgtcgtt ctcaaaatcg gtggagctgc atgacaaagt catcgggcat tatctgaaca
    15361 taaaacacta tcaataagtt ggagtcatta cccaattatg atagaattta caagctataa
    15421 ggttattgtc ctgggtttca agcattagtc catgcaagtt tttatgcttt gcccattcta
    15481 tagatatatt gataagcgcg ctgcctatgc cttgccccct gaaatcctta catacggcga
    15541 tatcttctat ataaaagata tattatctta tcagtattgt caatatattc aaggcaatct
    15601 gcctcctcat cctcttcatc ctcttcgtct tggtagcttt ttaaatatgg cgcttcatag
    15661 agtaattctg taaaggtcca attctcgttt tcatacctcg gtataatctt acctatcacc
    15721 tcaaatggtt cgctgggttt atcgcacccc cgaacacgag cacggcaccc gcgaccacta
    15781 tgccaagaat gcccaaggta aaaattgccg gccccgccat gaagtccgtg aatgccccga
    15841 cggccgaagt gaagggcagg ccgccaccca ggccgccgcc ctcactgccc ggcacctggt
    15901 cgctgaatgt cgatgccagc acctgcggca cgtcaatgct tccgggcgtc gcgctcgggc
    15961 tgatcgccca tcccgttact gccccgatcc cggcaatggc aaggactgcc agcgctgcca
    16021 tttttggggt gaggccgttc gcggccgagg ggcgcagccc ctggggggat gggaggcccg
    16081 cgttagcggg ccgggagggt tcgagaaggg ggggcacccc ccttcggcgt gcgcggtcac
    16141 gcgcacaggg cgcagccctg gttaaaaaca aggtttataa atattggttt aaaagcaggt
    16201 taaaagacag gttagcggtg gccgaaaaac gggcggaaac ccttgcaaat gctggatttt
    16261 ctgcctgtgg acagcccctc aaatgtcaat aggtgcgccc ctcatctgtc agcactctgc
    16321 ccctcaagtg tcaaggatcg cgcccctcat ctgtcagtag tcgcgcccct caagtgtcaa
    16381 taccgcaggg cacttatccc caggcttgtc cacatcatct gtgggaaact cgcgtaaaat
    16441 caggcgtttt cgccgatttg cgaggctggc cagctccacg tcgccggccg aaatcgagcc
    16501 tgcccctcat ctgtcaacgc cgcgccgggt gagtcggccc ctcaagtgtc aacgtccgcc
    16561 cctcatctgt cagtgagggc caagttttcc gcgaggtatc cacaacgccg gcggccgcgg
    16621 tgtctcgcac acggcttcga cggcgtttct ggcgcgtttg cagggccata gacggccgcc
    16681 agcccagcgg cgagggcaac cagcccgg
//
