LOCUS       sequence-296510-       11718 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Inducing plant cells to enter mitosis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11718)
  AUTHORS   Xu J, Lee YJ, Liu B
  TITLE     Establishment of a mitotic model system by transient expression of 
            the D-type cyclin in differentiated leaf cells of tobacco (Nicotiana
            benthamiana).
  JOURNAL   New Phytol. 2019 Nov 2. doi: 10.1111/nph.16309.
  PUBMED    31679162
REFERENCE   2  (bases 1 to 11718)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "New Phytol.
            2019 Nov 2. doi: 10.1111/nph.16309."
COMMENT     Sequence Label: pGWB660-CYCD3;1
FEATURES             Location/Qualifiers
     source          1..11718
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    69..93
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     281..303
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     295..312
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     296..312
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        827..1172
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     1115..1135
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    1194..1218
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2350..2374)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2385..3098
                     /codon_start=1
                     /product="monomeric derivative of red fluorescent protein 
                     from Entacmaea quadricolor (Merzlyak et al., 2007)"
                     /label=TagRFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     terminator      3120..3372
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3405..3421)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3405..3421)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3418..3440)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3429..3445
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3453..3459,3460..3477,3478..3483))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3498..3519
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      complement(3555..3807)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(3967..4518)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(4570..4749)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    5255..5279
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             5800..6591
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      6837..7425
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7326..7345
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7579..7596
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7611..7751
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7677..7696)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7837..7859
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8095..8289
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8355..9428)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(9857..10486)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 cgaacggata aaccttttca cgccctttta aatatccgat tattctaata aacgctcttt
       61 tctcttaggt ttacccgcca atatatcctg tcaaacactg atagtttaaa ctgaaggcgg
      121 gaaacgacaa tctgatccaa gctcaagctg ctctagcatt cgccattcag gctgcgcaac
      181 tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga
      241 tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa
      301 acgacggcca gtgccaagct tgcatgcctg caggtcccca gattagcctt ttcaatttca
      361 gaaagaatgc taacccacag atggttagag aggcttacgc agcaggtctc atcaagacga
      421 tctacccgag caataatctc caggaaatca aataccttcc caagaaggtt aaagatgcag
      481 tcaaaagatt caggactaac tgcatcaaga acacagagaa agatatattt ctcaagatca
      541 gaagtactat tccagtatgg acgattcaag gcttgcttca caaaccaagg caagtaatag
      601 agattggagt ctctaaaaag gtagttccca ctgaatcaaa ggccatggag tcaaagattc
      661 aaatagagga cctaacagaa ctcgccgtaa agactggcga acagttcata cagagtctct
      721 tacgactcaa tgacaagaag aaaatcttcg tcaacatggt ggagcacgac acacttgtct
      781 actccaaaaa tatcaaagat acagtctcag aagaccaaag ggcaattgag acttttcaac
      841 aaagggtaat atccggaaac ctcctcggat tccattgccc agctatctgt cactttattg
      901 tgaagatagt ggaaaaggaa ggtggctcct acaaatgcca tcattgcgat aaaggaaagg
      961 ccatcgttga agatgcctct gccgacagtg gtcccaaaga tggaccccca cccacgagga
     1021 gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa gcaagtggat tgatgtgata
     1081 tctccactga cgtaagggat gacgcacaat cccactatcc ttcgcaagac ccttcctcta
     1141 tataaggaag ttcatttcat ttggagagaa cacgggggac tctagagtta tcaacaagtt
     1201 tgtacaaaaa agcaggctta atggcgattc ggaaggagga agaaagtaga gaagaacaga
     1261 gcaattcgtt tcttcttgat gctctctact gcgaagaaga gaaatgggac gatgaaggag
     1321 aagaagttga agaaaactct tccttgtctt cttcttcttc tccattcgtt gttttgcaac
     1381 aagatttgtt ctgggaagat gaagatctgg ttacactctt ctccaaagaa gaagaacaag
     1441 gactcagctg tctcgatgat gtttatcttt ccacggatcg aaaagaagct gttggttgga
     1501 ttctgagagt caacgctcat tatggcttct ctactttagc agctgtttta gccataactt
     1561 atctcgataa gttcatctgt agctacagct tacagagaga caaaccatgg atgcttcagc
     1621 tcgtttctgt cgcgtgtctc tcattagctg ctaaagtcga agaaacccaa gtccctcttc
     1681 ttctagactt tcaagtggag gagacaaagt atgtgtttga agcaaaaacc atacagagaa
     1741 tggagctact gattctgtct actctcgagt ggaagatgca tctcattact ccaatttcgt
     1801 tcgtagacca cattatcagg agattgggac ttaagaacaa tgctcactgg gatttcctca
     1861 acaaatgcca ccgtctcctc ctctctgtaa tctccgattc aagatttgtc gggtacctcc
     1921 catcagtagt tgccgcagct accatgatgc gaattataga gcaagttgat ccctttgacc
     1981 ctctttcata ccaaactaat ctcctcggtg tccttaactt aaccaaggaa aaggtgaaaa
     2041 cttgctacga tctaatcctc caactaccag tggaccgcat cggtttacag atccaaatcc
     2101 aatcttccaa gaaacgcaag agtcacgatt catcatcatc gttgaacagt ccaagctgcg
     2161 tgattgatgc aaaccctttc aatagcgacg aaagctcaaa cgattcgtgg tcagcgagtt
     2221 cgtgcaaccc accaacgtcg tcgtcgtccc cgcagcaaca acctccattg aagaagatga
     2281 gaggagctga agagaatgag aagaagaagc cgattttgca tctgccatgg gcaatcgtag
     2341 ccactccaaa cccagctttc ttgtacaaag tggttgataa cagcatggtg tctaagggcg
     2401 aagagctgat taaggagaac atgcacatga agctgtacat ggagggcacc gtgaacaacc
     2461 accacttcaa gtgcacatcc gagggcgaag gcaagcccta cgagggcacc cagaccatga
     2521 gaatcaaggt ggtcgagggc ggccctctcc ccttcgcctt cgacatcctg gctaccagct
     2581 tcatgtacgg cagcagaacc ttcatcaacc acacccaggg catccccgac ttctttaagc
     2641 agtccttccc tgagggcttc acatgggaga gagtcaccac atacgaagac gggggcgtgc
     2701 tgaccgctac ccaggacacc agcctccagg acggctgcct catctacaac gtcaagatca
     2761 gaggggtgaa cttcccatcc aacggccctg tgatgcagaa gaaaacactc ggctgggagg
     2821 ccaacaccga gatgctgtac cccgctgacg gcggcctgga aggcagaagc gacatggccc
     2881 tgaagctcgt gggcgggggc cacctgatct gcaacttcaa gaccacatac agatccaaga
     2941 aacccgctaa gaacctcaag atgcccggcg tctactatgt ggaccacaga ctggaaagaa
     3001 tcaaggaggc cgacaaagag acctacgtcg agcagcacga ggtggctgtg gccagatact
     3061 gcgacctccc tagcaaactg gggcacaaac ttaattgagc ttagagctcg aatttccccg
     3121 atcgttcaaa catttggcaa taaagtttct taagattgaa tcctgttgcc ggtcttgcga
     3181 tgattatcat ataatttctg ttgaattacg ttaagcatgt aataattaac atgtaatgca
     3241 tgacgttatt tatgagatgg gtttttatga ttagagtccc gcaattatac atttaatacg
     3301 cgatagaaaa caaaatatag cgcgcaaact aggataaatt atcgcgcgcg gtgtcatcta
     3361 tgttactaga tcgggaattg gttccggaac caattcgtaa tcatgtcata gctgtttcct
     3421 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     3481 aaagcctggg gtgcctaatg agtgagctaa ctcacattag gctgaattag gcgcgcctat
     3541 ttctattaat tcccgatcta gtaacataga tgacaccgcg cgcgataatt tatcctagtt
     3601 tgcgcgctat attttgtttt ctatcgcgta ttaaatgtat aattgcggga ctctaatcat
     3661 aaaaacccat ctcataaata acgtcatgca ttacatgtta attattacat gcttaacgta
     3721 attcaacaga aattatatga taatcatcgc aagaccggca acaggattca atcttaagaa
     3781 actttattgc caaatgtttg aacgatcggg gaaattcgag ctggggacgt ccccagctcg
     3841 gtaccccctc ctgggcacgg tcgtacgcga tccgccgcgg ttccacctca ccattgcggg
     3901 tatgccagtt gagttcgatg agttcccgta ccagctccaa ctcgggcgat gccacgcgca
     3961 ctccgttcag atctcggtga cgggcaggac cggacggggc ggtaccggca ggctgaagtc
     4021 cagctgccag aaacccacgt catgccagtt cccgtgcttg aagccggccg cccgcagcat
     4081 gccgcggggg gcatatccga gcgcctcgtg catgcgcacg ctcgggtcgt tgggcagccc
     4141 gatgacagcg accacgctct tgaagccctg tgcctccagg gacttcagca ggtgggtgta
     4201 gagcgtggag cccagtcccg tccgctggtg gcggggggag acgtacacgg tcgactcggc
     4261 cgtccagtcg taggcgttgc gtgccttcca ggggcccgcg taggcgatgc cggcgacctc
     4321 gccgtccacc tcggcgacga gccagggata gcgctcccgc agacggacga ggtcgtccgt
     4381 ccactcctgc ggttcctgcg gctcggtacg gaagttgacc gtgcttgtct cgatgtagtg
     4441 gttgacgatg gtgcagaccg ccggcatgtc cgcctcggtg gcacggcgga tgtcggccgg
     4501 gcgtcgttct gggctcatat cttattgccc cccgggatca gatctggatt gagagtgaat
     4561 atgagactct aattggatac cgaggggaat ttatggaacg tcagtggagc atttttgaca
     4621 agaaatattt gctagctgat agtgacctta ggcgactttt gaacgcgcaa taatggtttc
     4681 tgacgtatgt gcttagctca ttaaactcca gaaacccgcg gctgagtggc tccttcaacg
     4741 ttgcggttct gtcagttcca aacgtaaaac ggcttgtccc gcgtcatcgg cgggggtcat
     4801 aacgtgactc ccttaattct ccgctcatga tcttgatccc ctgcgccatc agatccttgg
     4861 cggcaagaaa gccatccagt ttactttgca gggcttccca accttaccag agggcgcccc
     4921 agctggcaat tccggttcgc ttgctgtcca taaaaccgcc cagtctagct atcgccatgt
     4981 aagcccactg caagctacct gctttctctt tgcgcttgcg ttttcccttg tccagatagc
     5041 ccagtagctg acattcatcc ggggtcagca ccgtttctgc ggactggctt tctacgtgtt
     5101 ccgcttcctt tagcagccct tgcgccctga gtgcttgcgg cagcgtgaag ctgatccgtc
     5161 gagattaata gaaataaatt cagcctaatt cggcgttaat tcagtacatt aaaaacgtcc
     5221 gcaatgtgtt attaagttgt ctaagcgtca atttgtttac accacaatat atcctgccac
     5281 cagccagcca acagctcccc gaccggcagc tcggcacaaa atcaccactc gatacaggca
     5341 gcccatcagt ccgggacggc gtcagcggga gagccgttgt aaggcggcag actttgctca
     5401 tgttaccgat gctattcgga agaacggcaa ctaagctgcc gggtttgaaa cacggatgat
     5461 ctcgcggagg gtagcatgtt gattgtaacg atgacagagc gttgctgcct gtgatcaatt
     5521 cgggcacgaa cccagtggac ataagcctgt tcggttcgta agctgtaatg caagtagcgt
     5581 atgcgctcac gcaactggtc cagaaccttg accgaacgca gcggtggtaa cggcgcagtg
     5641 gcggttttca tggcttgtta tgactgtttt tttggggtac agtctatgcc tcgggcatcc
     5701 aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag caacgatgtt
     5761 acgcagcagg gcagtcgccc taaaacaaag ttaaacatca tgggggaagc ggtgatcgcc
     5821 gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg
     5881 ttgctggccg tacatttgta cggctccgca gtggatggcg gcctgaagcc acacagtgat
     5941 attgatttgc tggttacggt gaccgtaagg cttgatgaaa caacgcggcg agctttgatc
     6001 aacgaccttt tggaaacttc ggcttcccct ggagagagcg agattctccg cgctgtagaa
     6061 gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg
     6121 caatttggag aatggcagcg caatgacatt cttgcaggta tcttcgagcc agccacgatc
     6181 gacattgatc tggctatctt gctgacaaaa gcaagagaac atagcgttgc cttggtaggt
     6241 ccagcggcgg aggaactctt tgatccggtt cctgaacagg atctatttga ggcgctaaat
     6301 gaaaccttaa cgctatggaa ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg
     6361 cttacgttgt cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc
     6421 gctgccgact gggcaatgga gcgcctgccg gcccagtatc agcccgtcat acttgaagct
     6481 agacaggctt atcttggaca agaagaagat cgcttggcct cgcgcgcaga tcagttggaa
     6541 gaatttgtcc actacgtgaa aggcgagatc accaaggtag tcggcaaata atgtctagct
     6601 agaaattcgt tcaagccgac gccgcttcgc ggcgcggctt aactcaagcg ttagatgcac
     6661 taagcacata attgctcaca gccaaactat caggtcaagt ctgcttttat tatttttaag
     6721 cgtgcataat aagccctaca caaattggga gatatatcat gcatgaccaa aatcccttaa
     6781 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     6841 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     6901 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     6961 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag
     7021 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     7081 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     7141 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     7201 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     7261 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     7321 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     7381 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     7441 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     7501 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     7561 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg
     7621 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatggtgcac tctcagtaca
     7681 atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg
     7741 tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
     7801 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
     7861 tttcaccgtc atcaccgaaa cgcgcgaggc agggtgcctt gatgtgggcg ccggcggtcg
     7921 agtggcgacg gcgcggcttg tccgcgccct ggtagattgc ctggccgtag gccagccatt
     7981 tttgagcggc cagcggccgc gataggccga cgcgaagcgg cggggcgtag ggagcgcagc
     8041 gaccgaaggg taggcgcttt ttgcagctct tcggctgtgc gctggccaga cagttatgca
     8101 caggccaggc gggttttaag agttttaata agttttaaag agttttaggc ggaaaaatcg
     8161 ccttttttct cttttatatc agtcacttac atgtgtgacc ggttcccaat gtacggcttt
     8221 gggttcccaa tgtacgggtt ccggttccca atgtacggct ttgggttccc aatgtacgtg
     8281 ctatccacag gaaagagacc ttttcgacct ttttcccctg ctagggcaat ttgccctagc
     8341 atctgctccg tacattagga accggcggat gcttcgccct cgatcaggtt gcggtagcgc
     8401 atgactagga tcgggccagc ctgccccgcc tcctccttca aatcgtactc cggcaggtca
     8461 tttgacccga tcagcttgcg cacggtgaaa cagaacttct tgaactctcc ggcgctgcca
     8521 ctgcgttcgt agatcgtctt gaacaaccat ctggcttctg ccttgcctgc ggcgcggcgt
     8581 gccaggcggt agagaaaacg gccgatgccg ggatcgatca aaaagtaatc ggggtgaacc
     8641 gtcagcacgt ccgggttctt gccttctgtg atctcgcggt acatccaatc agctagctcg
     8701 atctcgatgt actccggccg cccggtttcg ctctttacga tcttgtagcg gctaatcaag
     8761 gcttcaccct cggataccgt caccaggcgg ccgttcttgg ccttcttcgt acgctgcatg
     8821 gcaacgtgcg tggtgtttaa ccgaatgcag gtttctacca ggtcgtcttt ctgctttccg
     8881 ccatcggctc gccggcagaa cttgagtacg tccgcaacgt gtggacggaa cacgcggccg
     8941 ggcttgtctc ccttcccttc ccggtatcgg ttcatggatt cggttagatg ggaaaccgcc
     9001 atcagtacca ggtcgtaatc ccacacactg gccatgccgg ccggccctgc ggaaacctct
     9061 acgtgcccgt ctggaagctc gtagcggatc acctcgccag ctcgtcggtc acgcttcgac
     9121 agacggaaaa cggccacgtc catgatgctg cgactatcgc gggtgcccac gtcatagagc
     9181 atcggaacga aaaaatctgg ttgctcgtcg cccttgggcg gcttcctaat cgacggcgca
     9241 ccggctgccg gcggttgccg ggattctttg cggattcgat cagcggccgc ttgccacgat
     9301 tcaccggggc gtgcttctgc ctcgatgcgt tgccgctggg cggcctgcgc ggccttcaac
     9361 ttctccacca ggtcatcacc cagcgccgcg ccgatttgta ccgggccgga tggtttgcga
     9421 ccgctcacgc cgattcctcg ggcttggggg ttccagtgcc attgcagggc cggcagacaa
     9481 cccagccgct tacgcctggc caaccgcccg ttcctccaca catggggcat tccacggcgt
     9541 cggtgcctgg ttgttcttga ttttccatgc cgcctccttt agccgctaaa attcatctac
     9601 tcatttattc atttgctcat ttactctggt agctgcgcga tgtattcaga tagcagctcg
     9661 gtaatggtct tgccttggcg taccgcgtac atcttcagct tggtgtgatc ctccgccggc
     9721 aactgaaagt tgacccgctt catggctggc gtgtctgcca ggctggccaa cgttgcagcc
     9781 ttgctgctgc gtgcgctcgg acggccggca cttagcgtgt ttgtgctttt gctcattttc
     9841 tctttacctc attaactcaa atgagttttg atttaatttc agcggccagc gcctggacct
     9901 cgcgggcagc gtcgccctcg ggttctgatt caagaacggt tgtgccggcg gcggcagtgc
     9961 ctgggtagct cacgcgctgc gtgatacggg actcaagaat gggcagctcg tacccggcca
    10021 gcgcctcggc aacctcaccg ccgatgcgcg tgcctttgat cgcccgcgac acgacaaagg
    10081 ccgcttgtag ccttccatcc gtgacctcaa tgcgctgctt aaccagctcc accaggtcgg
    10141 cggtggccca tatgtcgtaa gggcttggct gcaccggaat cagcacgaag tcggctgcct
    10201 tgatcgcgga cacagccaag tccgccgcct ggggcgctcc gtcgatcact acgaagtcgc
    10261 gccggccgat ggccttcacg tcgcggtcaa tcgtcgggcg gtcgatgccg acaacggtta
    10321 gcggttgatc ttcccgcacg gccgcccaat cgcgggcact gccctgggga tcggaatcga
    10381 ctaacagaac atcggccccg gcgagttgca gggcgcgggc tagatgggtt gcgatggtcg
    10441 tcttgcctga cccgcctttc tggttaagta cagcgataac cttcatgcgt tccccttgcg
    10501 tatttgttta tttactcatc gcatcatata cgcagcgacc gcatgacgca agctgtttta
    10561 ctcaaataca catcaccttt ttagacggcg gcgctcggtt tcttcagcgg ccaagctggc
    10621 cggccaggcc gccagcttgg catcagacaa accggccagg atttcatgca gccgcacggt
    10681 tgagacgtgc gcgggcggct cgaacacgta cccggccgcg atcatctccg cctcgatctc
    10741 ttcggtaatg aaaaacggtt cgtcctggcc gtcctggtgc ggtttcatgc ttgttcctct
    10801 tggcgttcat tctcggcggc cgccagggcg tcggcctcgg tcaatgcgtc ctcacggaag
    10861 gcaccgcgcc gcctggcctc ggtgggcgtc acttcctcgc tgcgctcaag tgcgcggtac
    10921 agggtcgagc gatgcacgcc aagcagtgca gccgcctctt tcacggtgcg gccttcctgg
    10981 tcgatcagct cgcgggcgtg cgcgatctgt gccggggtga gggtagggcg ggggccaaac
    11041 ttcacgcctc gggccttggc ggcctcgcgc ccgctccggg tgcggtcgat gattagggaa
    11101 cgctcgaact cggcaatgcc ggcgaacacg gtcaacacca tgcggccggc cggcgtggtg
    11161 gtgtcggccc acggctctgc caggctacgc aggcccgcgc cggcctcctg gatgcgctcg
    11221 gcaatgtcca gtaggtcgcg ggtgctgcgg gccaggcggt ctagcctggt cactgtcaca
    11281 acgtcgccag ggcgtaggtg gtcaagcatc ctggccagct ccgggcggtc gcgcctggtg
    11341 ccggtgatct tctcggaaaa cagcttggtg cagccggccg cgtgcagttc ggcccgttgg
    11401 ttggtcaagt cctggtcgtc ggtgctgacg cgggcatagc ccagcaggcc agcggcggcg
    11461 ctcttgttca tggcgtaatg tctccggttc tagtcgcaag tattctactt tatgcgacta
    11521 aaacacgcga caagaaaacg ccaggaaaag ggcagggcgg cagcctgtcg cgtaacttag
    11581 gacttgtgcg acatgtcgtt ttcagaagac ggctgcactg aacgtcagaa gccgactgca
    11641 ctatagcagc ggaggggttg gatcaaagta ctttgatccc gaggggaacc ctgtggttgg
    11701 catgcacata caaatgga
//
