LOCUS       sequence-296528-        9590 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CRISPR/Cas9 engineering with nourseothricin selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9590)
  AUTHORS   Barre B, Hallin J, Yue JX, Persson K, Mikhalev E, Irizar A, Holt S, 
            Thompson D, Molin M, Warringer J, Liti G
  TITLE     Intragenic repeat expansion in the cell wall protein gene HPF1 
            controls yeast chronological aging.
  JOURNAL   Genome Res. 2020 Apr 10. pii: gr.253351.119. doi: 
            10.1101/gr.253351.119.
  PUBMED    32277013
REFERENCE   2  (bases 1 to 9590)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome Res.
            2020 Apr 10. pii: gr.253351.119. doi: 10.1101/gr.253351.119."
COMMENT     Sequence Label: pL59-Nat
FEATURES             Location/Qualifiers
     source          1..9590
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..667
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     537..560
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
     primer_bind     complement(729..747)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     CDS             complement(join(1181..1972,1973..2041))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGTRVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1804..1823
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2042..2146)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      2249..2837
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2738..2757
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     gene            3001..4122
                     /label=natMX6
                     /note="yeast selectable marker conferring nourseothricin 
                     resistance"
     CDS             3349..3921
                     /codon_start=1
                     /gene="Streptomyces noursei nat1"
                     /product="nourseothricin acetyltransferase"
                     /label=NrsR
                     /note="confers resistance to nourseothricin"
                     /translation="MGTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDGF
                     TLREVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGWNR
                     RLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRMGFT
                     LCGLDTALYDGTASDGEQALYMSMPCP"
     CDS             4500..4523
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4527..4547
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4548..8648
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTYKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSITHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             8652..8672
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tcattatcaa tactgccatt tcaaagaata cgtaaataat taatagtagt gattttccta
       61 actttattta gtcaaaaaat tagcctttta attctgctgt aacccgtaca tgcccaaaat
      121 agggggcggg ttacacagaa tatataacat cgtaggtgtc tgggtgaaca gtttattctt
      181 ggcatccact aaatataatg gagcccgctt tttaagctgg catccagaaa aaaaaagaat
      241 cccagcacca aaatattgtt ttcttcacca accatcagtt cataggtcca ttctcttagc
      301 gcaactacag agaacagggg cacaaacagg caaaaaacgg gcacaacctc aatggagtga
      361 tgcaacctgc ctggagtaaa tgatgacaca aggcaattga cccacgcatg tatctatctc
      421 attttcttac accttctatt accttctgct ctctctgatt tggaaaaagc tgaaaaaaaa
      481 ggttgaaacc agttccctga aattattccc ctacttgact aataagtata taaagacggt
      541 aggtattgat tgtaattctg taaatctatt tcttaaactt cttaaattct acttttatag
      601 ttagtctttt ttttagtttt aaaacaccaa gaacttagtt tcgaataaac acacataaac
      661 aaacaaacga gacctgcgga ggtctccaca ggcccctttt cctttgtcga tatcatgtaa
      721 ttagttatgt cacgcttaca ttcacgccct ccccccacat ccgctctaac cgaaaaggaa
      781 ggagttagac aacctgaagt ctaggtccct atttattttt ttatagttat gttagtatta
      841 agaacgttat ttatatttca aatttttctt ttttttctgt acaaacgcgt gtacgcatgt
      901 aacattatac tgaaaacctt gcttgagaag gttttgggac aagcttacgt ctcacggatc
      961 gtatatgccg tagcgacaat ctaagaacta tgcgaggaca cgctagcagc tgtattcacg
     1021 tagacggata ggtatagcca gacatcagca gcatacttcg ggaaccgtag gccaccgagc
     1081 ggccgcgatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     1141 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     1201 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     1261 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     1321 gggacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     1381 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     1441 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     1501 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     1561 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     1621 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     1681 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     1741 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     1801 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     1861 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     1921 gtgtacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     1981 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     2041 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     2101 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     2161 aagtgccacc tgtcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     2221 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     2281 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     2341 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     2401 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     2461 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     2521 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     2581 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     2641 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     2701 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     2761 gtcctgtcgg gtttccccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     2821 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     2881 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtgcgg
     2941 actatatgtg aaggcatggc tatggcacgg cagacattcc gccagatcat caataggcac
     3001 gacatggagg cccagaatac cctccttgac agtcttgacg tgcgcagctc aggggcatga
     3061 tgtgactgtc gcccgtacat ttagcccata catccccatg tataatcatt tgcatccata
     3121 cattttgatg gccgcacggc gcgaagcaaa aattacggct cctcgctgca gacctgcgag
     3181 cagggaaacg ctcccctcac agacgcgttg aattgtcccc acgccgcgcc cctgtagaga
     3241 aatataaaag gttaggattt gccactgagg ttcttctttc atatacttcc ttttaaaatc
     3301 ttgctaggat acagttctca catcacatcc gaacataaac aaggatccat gggtactact
     3361 cttgacgaca cggcttaccg gtaccgcacc agtgtcccgg gggacgccga ggccatcgag
     3421 gcactggatg ggtccttcac caccgacacc gtcttccgcg tcaccgccac cggggacggc
     3481 ttcaccctgc gggaggtgcc ggtggacccg cccctgacca aggtgttccc cgacgacgaa
     3541 tcggacgacg aatcggacga cggggaggac ggcgacccgg actcccggac gttcgtcgcg
     3601 tacggggacg acggcgacct ggcgggcttc gtggtcgtct cgtactccgg ctggaaccgc
     3661 cggctgaccg tcgaggacat cgaggtcgcc ccggagcacc gggggcacgg ggtcgggcgc
     3721 gcgttgatgg ggctcgcgac ggagttcgcc cgcgagcggg gcgccgggca cctctggctg
     3781 gaggtcacca acgtcaacgc accggcgatc cacgcgtacc ggcggatggg gttcaccctc
     3841 tgcggcctgg acaccgccct gtacgacggc accgcctcgg acggcgagca ggcgctctac
     3901 atgagcatgc cctgccccta actcgaggac aataaaaaga ttcttgtttt caagaacttg
     3961 tcatttgtat agttttttta tattgtagtt gttctatttt aatcaaatgt tagcgtgatt
     4021 tatatttttt ttcgcctcga catcatctgc ccagatgcga agttaagtgc gcagaaagta
     4081 atatcatgcg tcaatcgtat gtgaatgctg gtcgctatac tggagctctc gagaaccctc
     4141 acctttcgag aggacgatgc ccgtgtctaa atgattcgac cagcctaaga atgttcaaca
     4201 atctataatc agtccatagt caacaagagc cctcaaagtt gatatcgttt agtgatctcc
     4261 ctcgagatga gcggaggatg gttgcttttt ggggagccct aagtggtacg tgcggcgagc
     4321 gggatgtttc cccattaggc aaaggacgac cgggtaaccc ctcgagaaaa aaaatttttc
     4381 gccgctaatc tggtgttata taaagctccc cctgctcctg gaattttttt ccttgtcaac
     4441 tcacaccgga aatcaaggca tttcattctg agtagttctt aaaaacataa tcaacaatgg
     4501 attataaaga tgacgatgac aaacctccaa aaaagaagag aaaggtcgat aagaaatact
     4561 caataggctt agatatcggc acaaatagcg tcggatgggc ggtgatcact gatgaatata
     4621 aggttccgtc taaaaagttc aaggttctgg gaaatacaga ccgccacagt atcaaaaaaa
     4681 atcttatagg ggctctttta tttgacagtg gagagacagc ggaagcgact cgtctcaaac
     4741 ggacagctcg tagaaggtat acacgtcgga agaatcgtat ttgttatcta caggagattt
     4801 tttcaaatga gatggcgaaa gtagatgata gtttctttca tcgacttgaa gagtcttttt
     4861 tggtggaaga agacaagaag catgaacgtc atcctatttt tggaaatata gtagatgaag
     4921 ttgcttatca tgagaaatat ccaactatct atcatctgcg aaaaaaattg gtagattcta
     4981 cttataaagc ggatttgcgc ttaatctatt tggccttagc gcatatgatt aagtttcgtg
     5041 gtcatttttt gattgaggga gatttaaatc ctgataatag tgatgtggac aaactattta
     5101 tccagttggt acaaacctac aatcaattat ttgaagaaaa ccctattaac gcaagtggag
     5161 tagatgctaa agcgattctt tctgcacgat tgagtaaatc aagacgatta gaaaatctca
     5221 ttgctcagct ccccggtgag aagaaaaatg gcttatttgg gaatctcatt gctttgtcat
     5281 tgggtttgac ccctaatttt aaatcaaatt ttgatttggc agaagatgct aaattacagc
     5341 tttcaaaaga tacttacgat gatgatttag ataatttatt ggcgcaaatt ggagatcaat
     5401 atgctgattt gtttttggca gctaagaatt tatcagatgc tattttactt tcagatatcc
     5461 taagagtaaa tactgaaata actaaggctc ccctatcagc ttcaatgatt aaacgctacg
     5521 atgaacatca tcaagacttg actcttttaa aagctttagt tcgacaacaa cttccagaaa
     5581 agtataaaga aatctttttt gatcaatcaa aaaacggata tgcaggttat attgatgggg
     5641 gagctagcca agaagaattt tataaattta tcaaaccaat tttagaaaaa atggatggta
     5701 ctgaggaatt attggtgaaa ctaaatcgtg aagatttgct gcgcaagcaa cggacctttg
     5761 acaacggctc tattacccat caaattcact tgggtgagct gcatgctatt ttgagaagac
     5821 aagaagactt ttatccattt ttaaaagaca atcgtgagaa gattgaaaaa atcttgactt
     5881 ttcgaattcc ttattatgtt ggtccattgg cgcgtggcaa tagtcgtttt gcatggatga
     5941 ctcggaagtc tgaagaaaca attaccccat ggaattttga agaagttgtc gataaaggtg
     6001 cttcagctca atcatttatt gaacgcatga caaactttga taaaaatctt ccaaatgaaa
     6061 aagtactacc aaaacatagt ttgctttatg agtattttac ggtttataac gaattgacaa
     6121 aggtcaaata tgttactgaa ggaatgcgaa aaccagcatt tctttcaggt gaacagaaga
     6181 aagccattgt tgatttactc ttcaaaacaa atcgaaaagt aaccgttaag caattaaaag
     6241 aagattattt caaaaaaata gaatgttttg atagtgttga aatttcagga gttgaagata
     6301 gatttaatgc ttcattaggt acctaccatg atttgctaaa aattattaaa gataaagatt
     6361 ttttggataa tgaagaaaat gaagatatct tagaggatat tgttttaaca ttgaccttat
     6421 ttgaagatag ggagatgatt gaggaaagac ttaaaacata tgctcacctc tttgatgata
     6481 aggtgatgaa acagcttaaa cgtcgccgtt atactggttg gggacgtttg tctcgaaaat
     6541 tgattaatgg tattagggat aagcaatctg gcaaaacaat attagatttt ttgaaatcag
     6601 atggttttgc caatcgcaat tttatgcagc tgatccatga tgatagtttg acatttaaag
     6661 aagacattca aaaagcacaa gtgtctggac aaggcgatag tttacatgaa catattgcaa
     6721 atttagctgg tagccctgct attaaaaaag gtattttaca gactgtaaaa gttgttgatg
     6781 aattggtcaa agtaatgggg cggcataagc cagaaaatat cgttattgaa atggcacgtg
     6841 aaaatcagac aactcaaaag ggccagaaaa attcgcgaga gcgtatgaaa cgaatcgaag
     6901 aaggtatcaa agaattagga agtcagattc ttaaagagca tcctgttgaa aatactcaat
     6961 tgcaaaatga aaagctctat ctctattatc tccaaaatgg aagagacatg tatgtggacc
     7021 aagaattaga tattaatcgt ttaagtgatt atgatgtcga tcacattgtt ccacaaagtt
     7081 tccttaaaga cgattcaata gacaataagg tcttaacgcg ttctgataaa aatcgtggta
     7141 aatcggataa cgttccaagt gaagaagtag tcaaaaagat gaaaaactat tggagacaac
     7201 ttctaaacgc caagttaatc actcaacgta agtttgataa tttaacgaaa gctgaacgtg
     7261 gaggtttgag tgaacttgat aaagctggtt ttatcaaacg ccaattggtt gaaactcgcc
     7321 aaatcactaa gcatgtggca caaattttgg atagtcgcat gaatactaaa tacgatgaaa
     7381 atgataaact tattcgagag gttaaagtga ttaccttaaa atctaaatta gtttctgact
     7441 tccgaaaaga tttccaattc tataaagtac gtgagattaa caattaccat catgcccatg
     7501 atgcgtatct aaatgccgtc gttggaactg ctttgattaa gaaatatcca aaacttgaat
     7561 cggagtttgt ctatggtgat tataaagttt atgatgttcg taaaatgatt gctaagtctg
     7621 agcaagaaat aggcaaagca accgcaaaat atttctttta ctctaatatc atgaacttct
     7681 tcaaaacaga aattacactt gcaaatggag agattcgcaa acgccctcta atcgaaacta
     7741 atggggaaac tggagaaatt gtctgggata aagggcgaga ttttgccaca gtgcgcaaag
     7801 tattgtccat gccccaagtc aatattgtca agaaaacaga agtacagaca ggcggattct
     7861 ccaaggagtc aattttacca aaaagaaatt cggacaagct tattgctcgt aaaaaagact
     7921 gggatccaaa aaaatatggt ggttttgata gtccaacggt agcttattca gtcctagtgg
     7981 ttgctaaggt ggaaaaaggg aaatcgaaga agttaaaatc cgttaaagag ttactaggga
     8041 tcacaattat ggaaagaagt tcctttgaaa aaaatccgat tgacttttta gaagctaaag
     8101 gatataagga agttaaaaaa gacttaatca ttaaactacc taaatatagt ctttttgagt
     8161 tagaaaacgg tcgtaaacgg atgctggcta gtgccggaga attacaaaaa ggaaatgagc
     8221 tggctctgcc aagcaaatat gtgaattttt tatatttagc tagtcattat gaaaagttga
     8281 agggtagtcc agaagataac gaacaaaaac aattgtttgt ggagcagcat aagcattatt
     8341 tagatgagat tattgagcaa atcagtgaat tttctaagcg tgttatttta gcagatgcca
     8401 atttagataa agttcttagt gcatataaca aacatagaga caaaccaata cgtgaacaag
     8461 cagaaaatat tattcattta tttacgttga cgaatcttgg agctcccgct gcttttaaat
     8521 attttgatac aacaattgat cgtaaacgat atacgtctac aaaagaagtt ttagatgcca
     8581 ctcttatcca tcaatccatc actggtcttt atgaaacacg cattgatttg agtcagctag
     8641 gaggtgaccc tccaaaaaag aagagaaagg tctgagcggt tttgtataac taaataatat
     8701 tggaaactaa atacgaatac ccaaattttt tatctaaatt ttgccgaaag attaaaatct
     8761 gcagagatat ccgaaacagg taaatggatg tttcaatccc tgtagtcagt caggaaccca
     8821 tattatatta cagtattagt cgccgcttag gcacgccttt aattagcaaa atcaaacctt
     8881 aagtgcatat gccgtataag ggaaactcaa agaactggca tcgcaaaaat gaaaacatac
     8941 gttgaaacta cggcaaagga ttggtcagat cgcttcatac agggaaagtt cggcatcaac
     9001 atctttggat aatatcagaa tgagaaagaa cagatacgca gtacgttttt tggtgagctc
     9061 tttgcacttc tttagttctt tccatcaata tcagtttttt aaacttttag gactaaaagt
     9121 gatgtttaac ttcaaaatgt ttaaaatttt gttcttcccg acgttcatta agaatactaa
     9181 tacactttaa taattatttt aatattttgt tctaaataat gacttttaat taaaaaagat
     9241 aaaatataaa aacatcataa taactcacca gaggttaaga acaaaaaaac aaattagata
     9301 tctgctaatc caatatagtt aaatcaatct ttccttggta taatgggtat attacatata
     9361 tttcaaggac cgacactcct accaaatatc taaaatttac catattaaca taacatgtat
     9421 ataaacgtca aatcataatc agcactaacg tctcacggat cgtatatgcc gtagcgacaa
     9481 tctaagaact atgcgaggac acgctagggc caaggcaaaa agattccttg attacgtaag
     9541 ggagttagaa tcattttgaa taaaaaacac gctttttcag ttcgagttta
//
