LOCUS       sequence-296535-         948 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Transient expression of human TMEM192. Dileucine motifs LL23-24 and 
            LL28-29 have been mutated to AA..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 948)
  AUTHORS   Adelmann CH, Traunbauer AK, Chen B, Condon KJ, Chan SH, Kunchok T, 
            Lewis CA, Sabatini DM
  TITLE     MFSD12 mediates the import of cysteine into melanosomes and 
            lysosomes.
  JOURNAL   Nature. 2020 Nov 18. pii: 10.1038/s41586-020-2937-x. doi: 
            10.1038/s41586-020-2937-x.
  PUBMED    33208952
REFERENCE   2  (bases 1 to 948)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2020 Nov 18. pii: 10.1038/s41586-020-2937-x. doi: 
            10.1038/s41586-020-2937-x."
COMMENT     Sequence Label: prk5-TMEM192-3xHA PM mutant
FEATURES             Location/Qualifiers
     source          1..948
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             829..855
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             868..894
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             907..933
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 atggcggcgg ggggcaggat ggaggacggt tccttggata tcacccagag tattgaagac
       61 gacccagcgg ctgatgccca ggctgctcca caccactcat tacaagctca ctttagaccc
      121 cgattccatc ctcttcctac agtcatcata gtgaatcttc tgtggtttat tcatctcgtg
      181 tttgttgttt tagcattttt aacaggtgtg ctttgttctt atcctaatcc aaatgaggac
      241 aagtgcccag gaaattacac aaacccattg aaagttcaga cggttataat ccttgggaaa
      301 gttattttgt ggattctcca tttactcctt gaatgctaca tccagtatca ccacagcaaa
      361 atcagaaacc gaggctataa cttgatctac cgatcaacaa ggcatctcaa gagacttgcg
      421 ttgatgatac agtcctctgg caacacagtg cttctcctca tactgtgcat gcagcactcc
      481 ttcccagagc ctggcagatt gtatcttgac ctcattctgg ccatcttggc actggaactc
      541 atctgttccc tgatatgtct cctcatttac acagtgaaaa tccggagatt taataaagct
      601 aaaccagagc ctgatatact tgaagaagaa aaaatctatg cttaccccag caatattacc
      661 tcggagactg gattcagaac tatttcaagc ctagaagaaa ttgttgaaaa gcaaggagac
      721 accattgaat acctgaagcg acacaatgcg ctgctgagta agcgattgtt ggctctcact
      781 tcctcagacc tgggctgtca gccaagtaga acgggaggtg gaggtagtta tccctatgac
      841 gtgcctgatt acgccggcac aggatcctac ccctatgatg tgcctgacta cgctggcagc
      901 gccggatacc cttatgatgt gcctgattat gctggaggga gcggctaa
//
