LOCUS       sequence-296653-        2201 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Diatom terminator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2201)
  AUTHORS   Jaramillo-Madrid AC, Abbriano R, Ashworth J, Fabris M, Pernice M, 
            Ralph PJ
  TITLE     Overexpression of Key Sterol Pathway Enzymes in Two Model Marine 
            Diatoms Alters Sterol Profiles in Phaeodactylum tricornutum.
  JOURNAL   Pharmaceuticals (Basel). 2020 Dec 21;13(12). pii: ph13120481. doi: 
            10.3390/ph13120481.
  PUBMED    33371196
REFERENCE   2  (bases 1 to 2201)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Pharmaceuticals (Basel)."; date: "2020-12-21"; volume: "13(12). 
            pii: ph13120481. doi"; pages: " 10.3390/ph13120481"
COMMENT     Sequence Label: EF_tTpEF2
FEATURES             Location/Qualifiers
     source          1..2201
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             696..1487
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1578..2166
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2067..2086
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtgggtgg
       61 aagggctcgg agttgtggta atctatgtat cctggtggtc tcgagcgagc agggttggtt
      121 agagataact aatgtctcac tctatacgac ctgtcaagtc actattgtag actcattgaa
      181 aaacattcac agtggatttt tttatctatt atctcatcaa tcgcgctacc attgttgcaa
      241 gaagatataa gcggagacct gagacgagac gagacagcct gagaatggat gcgagtaatg
      301 agctcggcac aaaatcacca ctcgatacag gcagcccatc agtccgggac ggcgtcagcg
      361 ggagagccgt tgtaaggcgg cagactttgc tcatgttacc gatgctattc ggaagaacgg
      421 caactaagct gccgggtttg aaacacggat gatctcgcgg agggtagcat gttgattgta
      481 acgatgacag agcgttgctg cctgtgatca ccgctcctct agagtcgact tgcaggcatg
      541 caagcttagc aagcgaaccg gaattgccag ctggggcgcc ctctggtaag gttgggaagc
      601 cctgcaaagt aaactggatg gctttcttgc cgccaaggat ctgatggcgc aggggatcaa
      661 tatctgatca agagacagga tgaggatcgt ttcgcatgag ggaagcggtg atcgccgaag
      721 tatcgactca actatcagag gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc
      781 tggccgtaca tttgtacggc tccgcagtgg atggcggcct gaagccacac agtgatattg
      841 atttgctggt tacggtgacc gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg
      901 accttttgga aacttcggct tcccctggag agagtgagat tctccgcgct gtagaagtca
      961 ccattgttgt gcacgacgac atcattccgt ggcgttatcc agctaagcgc gaactgcaat
     1021 ttggagaatg gcagcgcaat gacattcttg ctggtatctt cgagccagcc acgatcgaca
     1081 ttgatctggc tatcttgctg acaaaagcaa gagaacatag cgttgccttg gtaggtccag
     1141 cggcggagga actctttgat ccggttcctg aacaggatct atttgaggcg ctaaatgaaa
     1201 ccttaacgct atggaactcg ccgcccgact gggctggcga tgagcgaaat gtagtgctta
     1261 cgttgtcccg catttggtac agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg
     1321 ccgactgggc aatggagcgc ctgccggccc agtatcagcc cgtcatactt gaagctagac
     1381 aggcttatct tggacaagaa gaagatcgct tggcctcgcg cgcagatcag ttggaagaat
     1441 ttgtccacta cgtgaaaggc gagatcacca aggtagtcgg caaataataa tcacctagaa
     1501 tgcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     1561 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     1621 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     1681 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     1741 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     1801 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     1861 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     1921 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     1981 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     2041 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     2101 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     2161 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg c
//
