LOCUS       sequence-296662-       11233 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Over-expression of native 3-hydroxy-3-methyl glutaryl CoA reductase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11233)
  AUTHORS   Jaramillo-Madrid AC, Abbriano R, Ashworth J, Fabris M, Pernice M, 
            Ralph PJ
  TITLE     Overexpression of Key Sterol Pathway Enzymes in Two Model Marine 
            Diatoms Alters Sterol Profiles in Phaeodactylum tricornutum.
  JOURNAL   Pharmaceuticals (Basel). 2020 Dec 21;13(12). pii: ph13120481. doi: 
            10.3390/ph13120481.
  PUBMED    33371196
REFERENCE   2  (bases 1 to 11233)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Pharmaceuticals (Basel)."; date: "2020-12-21"; volume: "13(12). 
            pii: ph13120481. doi"; pages: " 10.3390/ph13120481"
COMMENT     Sequence Label: pL2-1 ConjEpi PtEF2bsd Pt49202HMGRVenusThrFLAG
FEATURES             Location/Qualifiers
     source          1..11233
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        69..255
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
     CDS             256..918
                     /codon_start=1
                     /gene="S. cerevisiae HIS3"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=HIS3
                     /note="yeast auxotrophic marker"
                     /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE
                     QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF
                     KEALGAVRGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHF
                     LESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM"
     CDS             complement(1076..1447)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELVRINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            1480..1589
                     /note="incP origin of transfer"
     CDS             2504..2899
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     CDS             join(6750..6752,6753..6755,6756..7268)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIE"
     primer_bind     complement(6795..6816)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7056..7075)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7403..7424
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             7464..7481
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             7482..7499
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             7500..7523
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     rep_origin      complement(8849..9437)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8929..8948)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(9528..10319)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_feature    join(10798..11233,1..68)
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
ORIGIN
        1 agagtaaatt cccaaattat tccatcatta aaagatacga ggcgcgtgta agttacaggc
       61 aagcgatcct agtacactct atattttttt atgcctcggt aatgattttc attttttttt
      121 tccacctagc ggatgactct ttttttttct tagcgattgg cattatcaca taatgaatta
      181 tacattatat aaagtaatgt gatttcttcg aagaatatac taaaaaatga gcaggcaaga
      241 taaacgaagg caaagatgac agagcagaaa gccctagtaa agcgtattac aaatgaaacc
      301 aagattcaga ttgcgatctc tttaaagggt ggtcccctag cgatagagca ctcgatcttc
      361 ccagaaaaag aggcagaagc agtagcagaa caggccacac aatcgcaagt gattaacgtc
      421 cacacaggta tagggtttct ggaccatatg atacatgctc tggccaagca ttccggctgg
      481 tcgctaatcg ttgagtgcat tggtgactta cacatagacg accatcacac cactgaagac
      541 tgcgggattg ctctcggtca agcttttaaa gaggccctag gggccgtgcg tggagtaaaa
      601 aggtttggat caggatttgc gcctttggat gaggcacttt ccagagcggt ggtagatctt
      661 tcgaacaggc cgtacgcagt tgtcgaactt ggtttgcaaa gggagaaagt aggagatctc
      721 tcttgcgaga tgatcccgca ttttcttgaa agctttgcag aggctagcag aattaccctc
      781 cacgttgatt gtctgcgagg caagaatgat catcaccgta gtgagagtgc gttcaaggct
      841 cttgcggttg ccataagaga agccacctcg cccaatggta ccaacgatgt tccctccacc
      901 aaaggtgttc ttatgtagtt ttacacagga gtctggactt gaccaatgga tcgtcttgcc
      961 ttgctcgtcg gtgatgtact tcaccagctc cgcgaagtcg cccttcttga tggagcgcat
     1021 ggggacgtgc ttggcaatca cgcgcacccc ccggccgttt tagcggctaa aaaagtcatg
     1081 gctctgccct cgggcggacc acgcccatca tgaccttgcc aagctcgtcc tgcttctctt
     1141 cgatcttcgc cagcagggcg aggatcgtgg catcaccgaa ccgcgccgtg cgcgggtcgt
     1201 cggtgagcca gagtttcagc aggccgccca ggcggcccag gtcgccattg atgcggacca
     1261 gctcgcggac gtgctcatag tccacgacgc ccgtgatttt gtagccctgg ccgacggcca
     1321 gcaggtaggc cgacaggctc atgccggccg ccgccgcctt ttcctcaatc gcccttcgtt
     1381 cgtctggaag gcagtacacc ttgataggtg ggctgccctt cctggttggc ttggtttcat
     1441 cagccatccg cttgccctca tctgttacgc cggcggtagc cggccagcct cgcagagcag
     1501 gattcccgtt gagcaccgcc aggtgcgaat aagggacagt gaagaaggaa cacccgctcg
     1561 cgggtgggcc tacttcacct atcctgcccg gctgacgccg ttggatacac caaggaaagt
     1621 ctacacgaac cctttggcaa aatcctgtat atcgtgcgaa aaaggatgga tataccgaaa
     1681 aaatcgctat aatgaccccg aagcagggtt atgcagcgga agatcgctag caaggagtcc
     1741 attttgacat gtttcctagc tagaaggaac agctattcat atgaattttc cgaggtcacg
     1801 ctgtgatgga tcgattcgtt cccgtcacag acgcaagcag cgaggtgatg gctggacggg
     1861 cacgcgtcac aatttagaac aacccggact cttgtgacgc gagctccggg tgcggaaaac
     1921 cctacggggt gaaaggagcc gcagcttcca cccatttact tgactgataa cccagtacac
     1981 acaacgctac cgtgacggaa atgccgcgcg ctgtgaagcc gtggtgaatc tttcgctgct
     2041 gctcgttctc tccacacaca gaattgcacg gcttgacgat tagcaaaaag cagaagaagc
     2101 agtgtcaaga tggtgagtgt cttgagtaca aaagtagcgc acggtagtgc agaatacggc
     2161 tgtgtagact atgcggcgtc ggagtagccg agatcccgta aaaaacggta ggaatcggag
     2221 gaatctcaat gagtgatcgt gctgcgtacg ccgcttccgt tggggaccat acgatcgcgt
     2281 agttcttgtt agcaacgaca tgcctttacg tcgcgaaggt ctttcaagta actaaaacaa
     2341 cggaatatgt caagattacc tcgatttgat ggattcgaat gaggatcggg atcaggagtt
     2401 ctattgacac tagttgattt cctcaatgcg aatgccgctc aatgtccccc tttctcatct
     2461 atgactgttt acttccctta ggtgaacttt acagttgaac agaatggcca agcctttgtc
     2521 tcaagaagaa tccaccctca ttgaaagagc aacggctaca atcaacagca tccccatctc
     2581 tgaagactac agcgtcgcca gcgcagctct ctctagcgac ggccgcatct tcactggtgt
     2641 caatgtatat cattttactg ggggaccttg tgcagaactc gtggtgctgg gcactgctgc
     2701 tgctgcggca gctggcaacc tgacttgtat cgtcgcgatc ggaaatgaga acaggggcat
     2761 cttgagcccc tgcggacggt gccgacaggt gcttctcgat ctgcatcctg ggatcaaagc
     2821 catagtgaag gacagtgatg gacagccgac ggcagttggg attcgtgaat tgctgccctc
     2881 tggttatgtg tgggagggct caggttaatg ataggcttac agaaaaacag actcataggg
     2941 tacttcgaac ataagaaaag attcattaca tgacggattt cagtttcgta ttcgtatagg
     3001 tttattgagt tgatgaacat ggcggtcaga tatgccaatc gcttttcttg gtacgagttt
     3061 gctaggcgct atacaggagt ccaaacggta cgtcagatcc cacgtcacta ccgcacccgg
     3121 ttattccagt gcgtaagctc gtagtggaag aacaggctgc ggtagtgacg tggaatttga
     3181 cactttccaa tcttcaagac ctctagccag caaagacttc ttgaatctgc aaggatattc
     3241 tatttagata taagtatata cttgacagtg aggggggtcg tgaccgtctc tcgctgggat
     3301 agagaagatg gcaaccattg aattgagaac tcgaaacttg agcttggttg gatcttgatc
     3361 attctgaatt tgcaggatag gttaacagta aaagtcaatc ttgcgctcgc catctaccag
     3421 aaagcacgtg ggtgcccaga tcctcccaac gagcgaacgg acggatgcta gtcgggggga
     3481 tctctgtggg ccattcaaac gaaaggcaga tggcatcgtt ccaagatcta ttggtcattg
     3541 ggcttctcat acagtcattg acaccacgat tgaagacaaa tttttgttga cagtgaaatc
     3601 gttgcggacg cgctccgatt caaaagtttc cctactcggt cgcgtcaacc gaccttcccc
     3661 tcgtgagttt acagcttgat acgccctatt tggcttccta tgtaaagtct ccagtgatca
     3721 tattaaagtc attggtcatg acactctcct tgttcaaaat ttggtaccat acaatgcgcg
     3781 gagcagtcag ttctcatttc cagcgatcgt taaaacaatt agagaaatgc cttacttctc
     3841 tctttatatc tgtattactc agagtcagtg agtagtacga atttccaacg tcctagctcc
     3901 aatttatggt ttcaaacaat ccgaattcgt tggtccacag tcaaggaatt cgcaagaatc
     3961 cctatttcac taggcgtgca ctatggggtc gcttttgagg ttctcatttg cggcgatcgt
     4021 taaaacgatt acagaaatgc tacacttcgc tctttatata tattactcat tgagcatcag
     4081 gaatgtccaa tgtcgtaact ccgattcatg gtttcaaaca atccgaattc gttggtccga
     4141 ggaatccgca aaaagtaatt gtagtctatg tacgaccgag tcgctggtga gggttttttg
     4201 tcgttggatt gctcgagcga attctccgag attgtacaga aagctcaact ttcggaaggc
     4261 atctatgtgg tccaaccaat ctattcaaat atatcaattg ttaagagcag agttcgatcc
     4321 tcactatgtg gaggggcatg gtgagattac ggagtgccga gacataagag ctccctgcaa
     4381 tcaagttgat ggacatgctt cgaaaatgcc atgactgaga acgagaacgc ccagatgctc
     4441 cgcggcacga gaactcgatg aacacgaacc gaatccaacg ccaaaaacgc gttgtccaaa
     4501 acattgcaac tctctctgat aaaaaccaaa acgccgcaaa ttgcaccagc ttttttggtt
     4561 gtcctacgcc tctttccgca ccaccaatga cggtgactat cagcagtagt attagtagaa
     4621 gtattggtag tagtacgact gccccaacac tgggaatgca ggtggatgcc cttgtccaac
     4681 aaatggataa cttgagcagt acgcagttgt acggattgat tgttggtttg accattctcg
     4741 tctcctttgt gttgctcggg tcttccgcgg acattcccgt ttctcttcga gacactacca
     4801 aagagacgtc gtcctcctcc tccacacccc gcaagacaac cacggtgtcg tcgtcgtcga
     4861 atcggggacc ggagccgcgc tggcacatct ttacttacgt caattacgcc attgtggcgt
     4921 gctttgtcgc ctccgttgca gaatttggac gcaacgcttc ggcgtacctt gctgccgacg
     4981 acaacgtcgt gctatacttt ctcgtcgctt ggagcgtctt tttgtgctac ttttttggtt
     5041 tctttggtgt ctcctttgtc cacgacgcgg acgctgcggt cgcgagtccc acaccaacca
     5101 agcccacggt ccggtacgtc cctgccttga tccattcggc cttgtctcga gatatacatg
     5161 tgtgtgcgtg tgtatacttg tgctatgcgt acttgtggtg tgtgcacatg cgtatacacc
     5221 agtacttata cgtacccgac cgagtttcct tcgttcgttc cacctcgtcc cggcccactt
     5281 ttcgtgttcc ggtgactccg tctcaccctc cttgcttatt ggttcgcagc gactctattt
     5341 ccaccaagac cagcgcgtcc aacactcttt ccgctcggca tcctcccgcc ccgtcggcac
     5401 ccgtatgcag tgatccctcc agtttcacac caatccatac cagcaagacc aacattacga
     5461 cactagataa cgctgccatc tgtcaactgg tgctcaccaa tcagatcaag gaccacgaac
     5521 tcgaaaaacg tctcgacgcg caccgcgccg tgcaggtccg ccgtttagtc gtcgcccaca
     5581 aactcgacac cttggaacac atcaacgcgc acgctctcga caatctgccc tcggaacctt
     5641 ccctcgacta cacccgcgta cacggcgcca attgcgaaat cgttgtgggc tacgtacccc
     5701 tcccggtggg actcgtcggt cccctcacgg tcaatggcga aaccgtctac gtgcccatgg
     5761 ccaccaccga aggatgtctc gtggcctcca cgaaccgcgg tgccaaggcc atcacggccg
     5821 ggggtggagc gaccgccgtc ctcctccggg acggtattac tcgggcgccc tgcgtacgca
     5881 tgccctcggc agcgcaagcc gctcatctca aattgtggtg tgaaacgccc caacacttta
     5941 gtacgctcca acgagccttt gaaagtacca cctcgttcgg gaaacttctc aagtgcgccc
     6001 ccacagtcgc tggacgtaac gtgtatctgc gcttgacctg cttttctgga gacgccatgg
     6061 gcatgaacat ggtttccaag ggcagtttgg ccgtcataga aacccttcaa cgagaatttc
     6121 cggaactcgt cctcgtcgcc ttgtctggca acatgtgcac cgataagaaa gccgccgcta
     6181 ccaactggct ggaaggacga ggaaaatcga tcgtcgtgga agcaaccatt cccaaggacg
     6241 tcgtcacaaa caccctcaag actacggtcc acagtatggt acaaactaat ttgcacaaga
     6301 atttgatcgg ttcggccatg gccggagctt tgggcggatt taacgcccac gcctccaaca
     6361 ttgttaccgc ggtctttttg gccacgggac aggaccccgc gcagaacgtg gagtccagta
     6421 attgcattac cctgttggaa gaaacggaag aaggcgactt gtggatttcc tgcaccatgc
     6481 ccagcattga ggtcggtacg gtcggtggcg gaacttcctt acccgcccaa gcggcctgtt
     6541 tgcaggccat gggtgtccgg ggcggaggcg ccactcccgg cgcccacgcc caaaaactgg
     6601 cacaaatcgt cgcgtccgct actttggccg gcgaactttc gctgctggcc gcactcgccg
     6661 cgaataccct cgtacaggcc cacatgcaac acaaccgcaa acccgccgcc aagtcaggta
     6721 acggagatct gggtggctcc ggcggccgga tggtgagcaa gggcgaggag ctgttcaccg
     6781 gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt
     6841 ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagctg atctgcacca
     6901 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgggctac ggcctgcagt
     6961 gcttcgcccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg
     7021 aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg
     7081 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact
     7141 tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg
     7201 tctatatcac cgccgacaag cagaagaacg gcatcaaggc caacttcaag atccgccaca
     7261 acatcgagga cggcggcgtg cagctcgccg accactacca gcagaacacc cccatcggcg
     7321 acggccccgt gctgctgccc gacaaccact acctgagcta ccagtccgcc ctgagcaaag
     7381 accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca
     7441 ctctcggcat ggacgagctg gcgctggtcc ctcgcggtag ccaccaccac catcaccacg
     7501 actacaagga tgatgacgat aagtgagctt ttcgatcgac gagcttacct tcacggccac
     7561 cgttcccgac cccgacgttg ttcaggcgat cattacgggt ggcagcgccg ctccgggagc
     7621 caccggtttg tacggcattg ccaccgcaga tgccgagtgc ccggccaacg agtgcatcca
     7681 tgtggacgtg attgtgtacg ccattttcgc gaatgaagcc acgaccaact tgattgtgga
     7741 aatcagcggc gacgcggtca tgtctgtggg acgtactgcc cgcaagttgc gtgcgaaagt
     7801 ccagcccaca cgcgagttgc aggagacgct ccagatgcaa gcctttcgta acgagattgt
     7861 cctcccccct ttgcctggtt cgggggccac gttggccaac gtgtgcgcgg cggcggtgcc
     7921 cttcgccgtg gccgtggtgg ccggtgcgct cgctctttaa aaagtgagag tttgtgtgtg
     7981 tgaaagacgc gtcgcgagac gctccttatt tccgtcggca tgtgtcttgt cgtggtccta
     8041 tgtgatcccc gggtcggttc acgggagttg ccgaagtaga gaataagaat tatataactg
     8101 tacagagata cagacatgtg ctgctattac gtccaacagt aggtatacac ctcccgggca
     8161 atagagtggg ccacatccat acacacacac ccgagttggt agattgattt cggttcgtcg
     8221 aatgccaaga atgtgcttcc tgaagaaaat ttgctccttc ccactccgtc cgtacgacca
     8281 tccgcccgtg tacagcgcgg cagaagtaga aacgagtacc aaggctctgt gtgtgtgtgt
     8341 gtaccgtttg gacgcgtcgg caaaggtacg gcagtcctcc gggtgtgtcc cgtactcgtg
     8401 gtaccaacga atctgcacaa ttgtgccgtt cggaggacac gccttcccgg atgcatgcat
     8461 ccggaagacc gtcgatgcga ttcgctacag aggaggggaa caaaggattg tgcagtgcca
     8521 tacgacaagt tcgcctcaat cgccaaaccc cttgactgtg cttcccgagc ggaaacattt
     8581 agattggcta ctacaaccgt tgctctccgg gcggataaga gaataaatct ggattcggca
     8641 gagtagtcgg ccttatcatc agttagatgt tgataatccc gttgggcagg tcacacgcta
     8701 ggtatacttg agaccacccc aggatacata gattaccaca actccgagcc cttccaccca
     8761 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     8821 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     8881 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     8941 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     9001 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     9061 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     9121 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     9181 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     9241 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg
     9301 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     9361 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     9421 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     9481 acgaaaactc acgttaaggg attttggtca tgcattctag gtgattatta tttgccgact
     9541 accttggtga tctcgccttt cacgtagtgg acaaattctt ccaactgatc tgcgcgcgag
     9601 gccaagcgat cttcttcttg tccaagataa gcctgtctag cttcaagtat gacgggctga
     9661 tactgggccg gcaggcgctc cattgcccag tcggcagcga catccttcgg cgcgattttg
     9721 ccggttactg cgctgtacca aatgcgggac aacgtaagca ctacatttcg ctcatcgcca
     9781 gcccagtcgg gcggcgagtt ccatagcgtt aaggtttcat ttagcgcctc aaatagatcc
     9841 tgttcaggaa ccggatcaaa gagttcctcc gccgctggac ctaccaaggc aacgctatgt
     9901 tctcttgctt ttgtcagcaa gatagccaga tcaatgtcga tcgtggctgg ctcgaagata
     9961 ccagcaagaa tgtcattgcg ctgccattct ccaaattgca gttcgcgctt agctggataa
    10021 cgccacggaa tgatgtcgtc gtgcacaaca atggtgactt ctacagcgcg gagaatctca
    10081 ctctctccag gggaagccga agtttccaaa aggtcgttga tcaaagctcg ccgcgttgtt
    10141 tcatcaagcc ttacggtcac cgtaaccagc aaatcaatat cactgtgtgg cttcaggccg
    10201 ccatccactg cggagccgta caaatgtacg gccagcaacg tcggttcgag atggcgctcg
    10261 atgacgccaa ctacctctga tagttgagtc gatacttcgg cgatcaccgc ttccctcatg
    10321 cgaaacgatc ctcatcctgt ctcttgatca gatattgatc ccctgcgcca tcagatcctt
    10381 ggcggcaaga aagccatcca gtttactttg cagggcttcc caaccttacc agagggcgcc
    10441 ccagctggca attccggttc gcttgctaag cttgcatgcc tgcaagtcga ctctagagga
    10501 gcggtgatca caggcagcaa cgctctgtca tcgttacaat caacatgcta ccctccgcga
    10561 gatcatccgt gtttcaaacc cggcagctta gttgccgttc ttccgaatag catcggtaac
    10621 atgagcaaag tctgccgcct tacaacggct ctcccgctga cgccgtcccg gactgatggg
    10681 ctgcctgtat cgagtggtga ttttgtgccg agctcattac tcgcatccat tctcaggctg
    10741 tctcgtctcg tctccgaggt ctcaggagaa tgaggagcta gctcgtcttc agcgagcatc
    10801 acgtgctata aaaataatta taatttaaat tttttaatat aaatatataa attaaaaata
    10861 gaaagtaaaa aaagaaatta aagaaaaaat agtttttgtt ttccgaagat gtaaaagact
    10921 ctagggggat cgccaacaaa tactaccttt tatcttgctc gtcctgctct caggtattaa
    10981 tgccgaattg tttcatcttg tctgtgtaga agaccacaca cgaaaatcct gtgattttac
    11041 attttactta tcgttaatcg aatgtatatc tatttaatct gcttttcttg tctaataaat
    11101 atatatgtaa agtacgcttt ttgttgaaat tttttaaacc tttgtttatt tttttttctt
    11161 cattccgtaa ctcttctacc ttctttattt actttctaaa atccaaatac aaaacataaa
    11221 aataaataaa cac
//
