LOCUS       sequence-296713-        3966 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pGEM T-easy Vector with S.mansoni gene Smp_302320 inserted.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3966)
  AUTHORS   McCusker P, Rohr CM, Chan JD
  TITLE     Schistosoma mansoni alter transcription of immunomodulatory gene 
            products following in vivo praziquantel exposure.
  JOURNAL   PLoS Negl Trop Dis. 2021 Mar 3;15(3):e0009200. doi: 
            10.1371/journal.pntd.0009200. eCollection 2021 Mar.
  PUBMED    33657133
REFERENCE   2  (bases 1 to 3966)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS Negl 
            Trop Dis."; date: "2021-03-3"; volume: "15(3):e0009200. doi"; pages:
            " 10.1371/journal.pntd.0009200. eCollection 2021 Mar"
COMMENT     Sequence Label: Smp_302320
FEATURES             Location/Qualifiers
     source          1..3966
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..456
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(88..107)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     298..319
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        534..638
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(639..707,708..1499)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(857..876)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1670..2258
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2159..2178
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2412..2429
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2546..2567
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2582..2587,2588..2605,2606..2612)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2620..2636
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2625..2647
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2644..2660
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2644..2660
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        2678..2696
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2678..2695
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      3741..3750
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      3745..3754
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(3784..3803)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3785..3803)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3810..3827)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3810..3826)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3819..3841)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
       61 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
      121 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
      181 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
      241 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
      301 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
      361 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
      421 acgcgaattt taacaaaata ttaacgctta caatttcctg atgcggtatt ttctccttac
      481 gcatctgtgc ggtatttcac accgcatcag gtggcacttt tcggggaaat gtgcgcggaa
      541 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
      601 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
      661 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
      721 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
      781 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
      841 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
      901 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
      961 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     1021 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     1081 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     1141 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     1201 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     1261 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     1321 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     1381 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     1441 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     1501 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     1561 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     1621 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     1681 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     1741 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     1801 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     1861 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     1921 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     1981 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     2041 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     2101 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     2161 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     2221 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     2281 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     2341 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     2401 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     2461 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     2521 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
     2581 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
     2641 acacaggaaa cagctatgac catgattacg ccaagctatt taggtgacac tatagaatac
     2701 tcaagctatg catccaacgc gttgggagct ctcccatatg gtcgacctgc aggcggccgc
     2761 gaattcacta gtgattgttg cttctccaat gacgcctcca ataccaatgt attgccgacc
     2821 acctgagcag aaacagataa cggatcaact gtctctggaa tcgcatacga ctgactgaac
     2881 tctgcaaatg aactcgactt tccatattga ccactcttct gcttatgatg acgtccacta
     2941 accacaacac gattcgaatc gacattcaca cacgagtctt caggcttgta cactgggtcc
     3001 actggaactt caacatacag tcgcttccca cctgaaccat catccacata cgacagacca
     3061 tgaacacctt tcgcaacaag agcttgtgat gcaggaactg ccttaatctg attgtccgat
     3121 ttcggtcgaa cagccacctg accagactcg ttcagcgtca acgactggtt ctgatcaacc
     3181 ttcactggag cctccaacat caaaacacca tcatctgtca tgcggcattt cagttgacta
     3241 tcatcaatac tcttcggcag ctgcaccata cgacagaact cccgactaca cttcccacca
     3301 tcggtcgtcg tctccttctt cgcgtgtacc gtcacacggt tctcactcga cgtcacattg
     3361 atgtcctgag gagcgaaacc ttgtgcatcg aatcgtactt tgaaatgtac cttgccatcc
     3421 tcacctactt cgtaggcgtc cttcaaaaag tcactcactg aaccggtcga cggcacatcc
     3481 attgagccca ttctctctcg gatatcctgg atatgacgat ccatttggtg cattaaagcg
     3541 aatggatttt ccagaatccc gtgggtgaaa gtatccaatg gtaatagtgc aaacattcca
     3601 cgtctcaaac gtcgcatttc ttcatcccat cgcctccacg aggagttggt ccaactgtcc
     3661 actgtactcg gccagtcttc cgtgtatggt ccgattaagt tgccacgatg agtaccactc
     3721 ccgtgaatcg aattcccgcg gccgccatgg cggccgggag catgcgacgt cgggcccaat
     3781 tcgccctata gtgagtcgta ttacaattca ctggccgtcg ttttacaacg tcgtgactgg
     3841 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
     3901 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
     3961 gaatgg
//
