LOCUS       sequence-296738-        4029 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Olfr644 in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4029)
  AUTHORS   Inagaki S, Iwata R, Iwamoto M, Imai T
  TITLE     Widespread Inhibition, Antagonism, and Synergy in Mouse Olfactory 
            Sensory Neurons In Vivo.
  JOURNAL   Cell Rep. 2020 Jun 30;31(13):107814. doi: 
            10.1016/j.celrep.2020.107814.
  PUBMED    32610120
REFERENCE   2  (bases 1 to 4029)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2020-06-30"; volume: "31(13):107814. doi"; pages: " 
            10.1016/j.celrep.2020.107814"
COMMENT     Sequence Label: pME18S-F-R-Olfr644
FEATURES             Location/Qualifiers
     source          1..4029
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(13..33)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        join(18..347,348..363,364..632)
                     /label=SR-alpha promoter
                     /note="hybrid promoter consisting of the SV40 early 
                     promoter plus part of the long terminal repeat of human 
                     T-lymphotrophic virus 1"
     rep_origin      198..333
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     LTR             364..632
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     intron          662..755
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     CDS             803..826
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             848..886
                     /codon_start=1
                     /product="segment of bovine rhodopsin containing a signal 
                     for N-linked glycosylation (Favaloro et al., 2008)"
                     /label=opsin tag
                     /note="The asparagine in the NKT tripeptide will be 
                     glycosylated if this sequence is exposed to the ER lumen."
                     /translation="GPNFYVPFSNKTG"
     polyA_signal    1934..2068
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1971..1990)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2025..2044
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2107..2124)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2278..2866)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2358..2377)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3037..3828,3829..3897))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3660..3679
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3898..4002)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aagcttggct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
       61 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
      121 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
      181 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
      241 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
      301 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcctccgat
      361 cgaggggctc gcatctctcc ttcacgcgcc cgccgcccta cctgaggccg ccatccacgc
      421 cggttgagtc gcgttctgcc gcctcccgcc tgtggtgcct cctgaactgc gtccgccgtc
      481 taggtaagtt taaagctcag gtcgagaccg ggcctttgtc cggcgctccc ttggagccta
      541 cctagactca gccggctctc cacgctttgc ctgaccctgc ttgctcaact ctacgtcttt
      601 gtttcgtttt ctgttctgcg ccgttacaga tccaagctct gaaaaaccag aaagttaact
      661 ggtaagttta gtctttttgt cttttatttc aggtcccgga tccggtggtg caaatcaaag
      721 aactgctcct cagtggatgt tgcctttact tctaggcctg tacggaagtg ttacttctgc
      781 tctaaaagct gcggaattca tggactacaa ggacgacgac gacaaggaat tcatgaacgg
      841 gaccgagggc ccaaacttct acgtgccttt ctccaacaag acgggcgtgg tgctcgagat
      901 gggaggtgaa gcccacaaca gctctgggtt gcctcccttc atcttgacag gactgccagg
      961 gatggagacc tcccagcact ggttgttcct gctccttggt gttctttaca gcgtctccat
     1021 agtgggcaat gccctgattc tgttcatcat caaggaggaa gagagtcttc accagcccat
     1081 gtactacttc ctatctctgc tgtcaggaaa tgaccttggt gtgtctttct ccacactgcc
     1141 tacagttctg ggagtgtttt gttttcactt aagagagatc agttttaact cctgcatgtc
     1201 ccaaatgttc tttatccatc ttttctcttt catggagtct gggattctgc tggccatgag
     1261 ctttgatcgc tatgtagcca tctgtaaccc actgcgctat tccacagtac tcactgatgc
     1321 ccgagtgatg tggatgggtg tatgtgtttt ccttcgaagt ttctgcatga ttttcccatt
     1381 acctttcctt ctgaagaggt tgcccttctg caaggctaat gtgctctctc atgcctactg
     1441 tctgcatcct gatctgattc gactgccttg tggtgacact tccattaata atatatttgg
     1501 tctgtccatt gtcatctcta cctttgggct ggattctgca ctcattttcc tttcttatgt
     1561 tctcatactt cgctcagtgc ttgctattgc ctcccgggaa gaaaggatga aaacactgaa
     1621 tacatgtgtg tcacacctgt gtgctgtgct catcttctat gtgcctaagg taggtgtgtc
     1681 catgtttgct cgctatggga ggcatgcccc tcactatgta cacacactct tgtcactcat
     1741 ctatctcttt gttcctccaa tgctcaaccc tgtcatctac tccatcaaaa ccaaggagat
     1801 tcgtcggagg ttttgcaaaa tcctgctggg gaacaaattt tgaccgcggg cggccgctag
     1861 actagtctag agaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc
     1921 aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     1981 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     2041 catcaatgta tcttatcatg tctggatccc cgggtaccga gctcgaatta attcctcttc
     2101 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     2161 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     2221 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     2281 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     2341 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     2401 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     2461 ggcgctttct caatgctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     2521 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     2581 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     2641 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     2701 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     2761 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     2821 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     2881 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     2941 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     3001 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     3061 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     3121 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     3181 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     3241 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     3301 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     3361 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     3421 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     3481 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     3541 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     3601 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     3661 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     3721 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     3781 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     3841 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     3901 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     3961 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     4021 gccacctgc
//
