LOCUS       sequence-296741-        8727 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive PGK driven expression of leucine zipper with C terminal
            dCpf1 (Cas12a) 406-split half fused to miniVPR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8727)
  AUTHORS   Kempton HR, Goudy LE, Love KS, Qi LS
  TITLE     Multiple Input Sensing and Signal Integration Using a Split Cas12a 
            System.
  JOURNAL   Mol Cell. 2020 Jan 30. pii: S1097-2765(20)30037-X. doi: 
            10.1016/j.molcel.2020.01.016.
  PUBMED    32027839
REFERENCE   2  (bases 1 to 8727)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2020 Jan 30. pii: S1097-2765(20)30037-X. doi: 
            10.1016/j.molcel.2020.01.016."
COMMENT     Sequence Label: pSLQ8541 PB-PGK-RR12EE345L-C term (406)
            dLbCpf...
FEATURES             Location/Qualifiers
     source          1..8727
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..500
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(36..59)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     433..452
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             3172..3198
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3178..3204
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             3217..3237
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3244..3264
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3301..3450
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             3457..3477
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3610..3966
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALL"
     CDS             4201..4911
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4346..4364)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4614..4633
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     promoter        complement(4930..4948)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4931..4948)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4968..4985)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4974..5198
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   5414..5476
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(5527..5547)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(5527..5545)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5566..5582)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5566..5582)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5579..5601)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5590..5606
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5614..5620,5621..5638,5639..5644))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5659..5680
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5797..5814)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5968..6556)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6048..6067)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6727..7518,7519..7587))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7350..7369
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7588..7692)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(7718..8173)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7855..7876)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8067..8086
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8300..8322
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8314..8331
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8315..8331
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8341..8360
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        8341..8359
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   8389..8423
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
ORIGIN
        1 gggtagggga ggcgcttttc ccaaggcagt ctggagcatg cgctttagca gccccgctgg
       61 gcacttggcg ctacacaagt ggcctctggc ctcgcacaca ttccacatcc accggtaggc
      121 gccaaccggc tccgttcttt ggtggcccct tcgcgccacc ttctactcct cccctagtca
      181 ggaagttccc ccccgccccg cagctcgcgt cgtgcaggac gtgacaaatg gaagtagcac
      241 gtctcactag tctcgtgcag atggacagca ccgctgagca atggaagcgg gtaggccttt
      301 ggggcagcgg ccaatagcag ctttgctcct tcgctttctg ggctcagagg ctgggaaggg
      361 gtgggtccgg gggcgggctc aggggcgggc tcaggggcgg ggcgggcgcc cgaaggtcct
      421 ccggaggccc ggcattctgc acgcttcaaa agcgcacgtc tgccgcgctg ttctcctctt
      481 cctcatctcc gggcctttcg agaattcggt accatgctgg agatccgcgc cgccttcctg
      541 cgccagcgca acaccgccct gcgcaccgag gtggccgagc tggagcagga ggtgcagcgc
      601 ctggagaacg aggtgagcca gtacgagacc cgctacggcc ccctgggcgg cggcaaggct
      661 agcggaggag gtggtggagg tggaggtgga ggtcatatgg atctgtctgt ggtggagaag
      721 ctgaaggaga tcatcatcca gaaggtggat gagatctaca aggtgtatgg ctcctctgag
      781 aagctgttcg acgccgattt tgtgctggag aagagcctga agaagaacga cgccgtggtg
      841 gccatcatga aggacctgct ggattctgtg aagagcttcg agaattacat caaggccttc
      901 tttggcgagg gcaaggagac aaacagggac gagtccttct atggcgattt tgtgctggcc
      961 tacgacatcc tgctgaaggt ggaccacatc tacgatgcca tccgcaatta tgtgacccag
     1021 aagccctact ctaaggataa gttcaagctg tattttcaga accctcagtt catgggcggc
     1081 tgggacaagg ataaggagac agactatcgg gccaccatcc tgagatacgg ctccaagtac
     1141 tatctggcca tcatggataa gaagtacgcc aagtgcctgc agaagatcga caaggacgat
     1201 gtgaacggca attacgagaa gatcaactat aagctgctgc ccggccctaa taagatgctg
     1261 ccaaaggtgt tcttttctaa gaagtggatg gcctactata accccagcga ggacatccag
     1321 aagatctaca agaatggcac attcaagaag ggcgatatgt ttaacctgaa tgactgtcac
     1381 aagctgatcg acttctttaa ggatagcatc tcccggtatc caaagtggtc caatgcctac
     1441 gatttcaact tttctgagac agagaagtat aaggacatcg ccggctttta cagagaggtg
     1501 gaggagcagg gctataaggt gagcttcgag tctgccagca agaaggaggt ggataagctg
     1561 gtggaggagg gcaagctgta tatgttccag atctataaca aggacttttc cgataagtct
     1621 cacggcacac ccaatctgca caccatgtac ttcaagctgc tgtttgacga gaacaatcac
     1681 ggacagatca ggctgagcgg aggagcagag ctgttcatga ggcgcgcctc cctgaagaag
     1741 gaggagctgg tggtgcaccc agccaactcc cctatcgcca acaagaatcc agataatccc
     1801 aagaaaacca caaccctgtc ctacgacgtg tataaggata agaggttttc tgaggaccag
     1861 tacgagctgc acatcccaat cgccatcaat aagtgcccca agaacatctt caagatcaat
     1921 acagaggtgc gcgtgctgct gaagcacgac gataacccct atgtgatcgg catcgccagg
     1981 ggcgagcgca atctgctgta tatcgtggtg gtggacggca agggcaacat cgtggagcag
     2041 tattccctga acgagatcat caacaacttc aacggcatca ggatcaagac agattaccac
     2101 tctctgctgg acaagaagga gaaggagagg ttcgaggccc gccagaactg gacctccatc
     2161 gagaatatca aggagctgaa ggccggctat atctctcagg tggtgcacaa gatctgcgag
     2221 ctggtggaga agtacgatgc cgtgatcgcc ctggaggacc tgaactctgg ctttaagaat
     2281 agccgcgtga aggtggagaa gcaggtgtat cagaagttcg agaagatgct gatcgataag
     2341 ctgaactaca tggtggacaa gaagtctaat ccttgtgcaa caggcggcgc cctgaagggc
     2401 tatcagatca ccaataagtt cgagagcttt aagtccatgt ctacccagaa cggcttcatc
     2461 ttttacatcc ctgcctggct gacatccaag atcgatccat ctaccggctt tgtgaacctg
     2521 ctgaaaacca agtataccag catcgccgat tccaagaagt tcatcagctc ctttgacagg
     2581 atcatgtacg tgcccgagga ggatctgttc gagtttgccc tggactataa gaacttctct
     2641 cgcacagacg ccgattacat caagaagtgg aagctgtact cctacggcaa ccggatcaga
     2701 atcttccgga atcctaagaa gaacaacgtg ttcgactggg aggaggtgtg cctgaccagc
     2761 gcctataagg agctgttcaa caagtacggc atcaattatc agcagggcga tatcagagcc
     2821 ctgctgtgcg agcagtccga caaggccttc tactctagct ttatggccct gatgagcctg
     2881 atgctgcaga tgcggaacag catcacaggc cgcaccgacg tggattttct gatcagccct
     2941 gtgaagaact ccgacggcat cttctacgat agccggaact atgaggccca ggagaatgcc
     3001 atcctgccaa agaacgccga cgccaatggc gcctataaca tcgccagaaa ggtgctgtgg
     3061 gccatcggcc agttcaagaa ggccgaggac gagaagctgg ataaggtgaa gatcgccatc
     3121 tctaacaagg agtggctgga gtacgcccag accagcgtga agcacggatc ctatccctat
     3181 gacgtgcccg attatgccag cctgggcagc ggctccccca agaaaaaacg caaggtggaa
     3241 gatcctaaga aaaagcggaa agtggacggc attggtagtg ggagcaacgg cagcagcctc
     3301 gatgctttag acgattttga cttagatatg cttggttcag acgcgttaga cgacttcgac
     3361 ctagacatgt taggctcaga tgcattggac gacttcgatt tagatatgtt gggctccgat
     3421 gccctagatg actttgatct agatatgcta ggtagtccca aaaagaagag gaaagtggga
     3481 tccgtccttc cgcaagcgcc tgccccagcg cctgcaccgg caatggtgtc cgccctcgca
     3541 caggcccctg cgcccgtccc cgtgctcgcg cctggaccgc cccaggcggt cgctccaccg
     3601 gctccgaagc cgacgcaggc cggagaggga acactctccg aagcacttct tcaactccag
     3661 tttgatgacg aggatcttgg agcactcctt ggaaactcga cagaccctgc ggtgtttacc
     3721 gacctcgcgt cagtagataa ctccgaattt cagcagcttt tgaaccaggg tatcccggtc
     3781 gcgccacata caacggagcc catgttgatg gaataccccg aagcaatcac gagacttgtg
     3841 acgggagcgc agcggcctcc cgatcccgca cccgcacctt tgggggcacc tggcctccct
     3901 aacggacttt tgagcggcga cgaggatttc tcctccatcg ccgatatgga tttctcagcc
     3961 ttgctgtcag gctctggcag cggcagcgac ctttcccatc cgcccccaag gggccatctg
     4021 gatgagctga caaccacact tgagtccatg accgaggatc tgaacctgga ctcacccctg
     4081 accccggaat tgaacgagat tctggatacc ttcctgaacg acgagtgcct cttgcatgcc
     4141 atgcatatca gcacaggact gtccatcttc gacacatctc tgtttggcag cagcggatcc
     4201 atggtgagca agggcgagga ggataacatg gccatcatca aggagttcat gcgcttcaag
     4261 gtgcacatgg agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc
     4321 cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc
     4381 ttcgcctggg acatcctgtc ccctcagttc atgtacggct ccaaggccta cgtgaagcac
     4441 cccgccgaca tccccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc
     4501 gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac
     4561 ggcgagttca tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta
     4621 atgcagaaga agaccatggg ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc
     4681 gccctgaagg gcgagatcaa gcagaggctg aagctgaagg acggcggcca ctacgacgct
     4741 gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc
     4801 aacatcaagt tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgaa
     4861 cgcgccgagg gccgccactc caccggcggc atggacgagc tgtacaagta actcgagaga
     4921 gggccctatt ctatagtgtc acctaaatgc tagagctcgc tgatcagcct cgactgtgcc
     4981 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg
     5041 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag
     5101 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga
     5161 caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggtcg aggcggccgc
     5221 tgatcacaat tgtctagtta aaagttttgt tactttatag aagaaatttt gagtttttgt
     5281 ttttttttaa taaataaata aacataaata aattgtttgt tgaatttatt attagtatgt
     5341 aagtgtaaat ataataaaac ttaatatcta ttcaaattaa taaataaacc tcgatataca
     5401 gaccgataaa acacatgcgt caattttacg catgattatc tttaacgtac gtcacaatat
     5461 gattatcttt ctagggttaa tctagtatac gcgtatgcgg ccgcttaatt aatccagctt
     5521 ttgttccctt tagtgagggt taattgcgcg cttggcgtaa tcatggtcat agctgtttcc
     5581 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     5641 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     5701 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     5761 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     5821 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     5881 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     5941 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     6001 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     6061 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     6121 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     6181 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     6241 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     6301 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     6361 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     6421 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     6481 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     6541 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     6601 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     6661 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     6721 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     6781 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     6841 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     6901 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     6961 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     7021 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     7081 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     7141 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     7201 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     7261 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     7321 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     7381 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     7441 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     7501 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     7561 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     7621 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     7681 aggggttccg cgcacatttc cccgaaaagt gccacctaaa ttgtaagcgt taatattttg
     7741 ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc
     7801 ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt
     7861 tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc
     7921 tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg
     7981 tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga
     8041 aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg
     8101 ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg
     8161 ctacagggcg cgtcccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg
     8221 cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt
     8281 tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgagcgcgcg
     8341 taatacgact cactataggg cgaattgggg cgcgccattc tagattaacc ctagaaagat
     8401 agtctgcgta aaattgacgc atgcattctt gaaatattgc tctctctttc taaatagcgc
     8461 gaatccgtcg ctgtgcattt aggacatctc agtcgccgct tggagctccc gtgaggcgtg
     8521 cttgtcaatg cggtaagtgt cactgatttt gaactataac gaccgcgtga gtcaaaatga
     8581 cgcatgatta tcttttacgt gacttttaag atttaactca tacgataatt atattgttat
     8641 ttcatgttct acttacgtga taacttatta tatatatatt ttcttgttat agatatcaac
     8701 tagaatgcta gcatgggccc agatctc
//
