LOCUS       sequence-296742-       12013 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dox-inducible expression of anti-Cpf1 AcrVA1. Constitutive EF1a 
            driven rtTA and GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12013)
  AUTHORS   Kempton HR, Goudy LE, Love KS, Qi LS
  TITLE     Multiple Input Sensing and Signal Integration Using a Split Cas12a 
            System.
  JOURNAL   Mol Cell. 2020 Jan 30. pii: S1097-2765(20)30037-X. doi: 
            10.1016/j.molcel.2020.01.016.
  PUBMED    32027839
REFERENCE   2  (bases 1 to 12013)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2020 Jan 30. pii: S1097-2765(20)30037-X. doi: 
            10.1016/j.molcel.2020.01.016."
COMMENT     Sequence Label: pSLQ8702 pHR-TRE3G-AcrVA1-EF1a-sfGFP-T2A-rtta-...
FEATURES             Location/Qualifiers
     source          1..12013
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..234
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             419..463
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    748..865
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    960..978
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    996..1014
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1032..1050
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1068..1086
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1104..1122
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1140..1158
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1176..1194
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     1239..1263
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          2033..2955
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     2912..2932
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             join(2980..2982,2983..3693)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             2980..3693
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Nager et al., 2011)"
                     /label=superfolder GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(3010..3038)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     3633..3653
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     CDS             3703..3756
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             3769..4515
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    4575..5163
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4628..4648)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5046..5057)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5166..5182)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5167..5183)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5273..5506
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(5531..5549)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(5532..5549)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5839..5858)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(6072..6091)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6191..6213
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6251..6269)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6337..6441
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6442..6510,6511..7302)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6660..6679)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7473..8061
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7962..7981
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8215..8232
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(8302..8322)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8307..8636
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8487..8622
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          9770..9835
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             9965..9985
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    10410..10544
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10447..10466)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10501..10520
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             10712..11345
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    11392..11517
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
ORIGIN
        1 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat
       61 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt
      121 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca
      181 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat
      241 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag
      301 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat
      361 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa
      421 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat
      481 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt
      541 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt
      601 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga
      661 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta
      721 tcgccaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag
      781 tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag aattacaaaa
      841 acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg
      901 gatcgataag cttgatatcg aattacatat gctgatatct ccaccccaca gtggtttact
      961 ccctatcagt gatagagaac gtatgaagag tttactccct atcagtgata gagaacgtat
     1021 gcagacttta ctccctatca gtgatagaga acgtataagg agtttactcc ctatcagtga
     1081 tagagaacgt atgaccagtt tactccctat cagtgataga gaacgtatct acagtttact
     1141 ccctatcagt gatagagaac gtatatccag tttactccct atcagtgata gagaacgtat
     1201 aagctttagg cgtgtacggt gggcgcctat aaaagcagag ctcgtttagt gaaccgtcag
     1261 atcgcctgga gcaattccac aacacttttg tcttatacca actttccgta ccacttccta
     1321 ccctcgtaaa gaattcccac catgtcaaag gctatgtatg aagccaaaga acgatatgcg
     1381 aagaagaaga tgcaggagaa cactaagata gacactctca ccgatgagca gcatgacgct
     1441 ctcgcccagc tttgcgcgtt tagacataag tttcattcta acaaagattc cctgtttctt
     1501 tccgaatcag cgttcagtgg ggagtttagt ttcgagatgc agtctgacga gaactccaag
     1561 ttgagggaag taggactccc cacaatcgaa tggtcctttt acgacaattc tcacataccc
     1621 gacgacagct ttagagagtg gttcaatttt gctaactatt ctgagctttc agaaacaata
     1681 caagagcagg gtcttgaact cgacctggat gatgacgaaa cctacgagct ggtctatgat
     1741 gagctttata cagaggcgat gggagaatac gaggaattga accaggatat agagaaatac
     1801 cttcgcagaa tagatgagga acatggcacg caatactgtc caactggttt tgcgcggctg
     1861 agataaggat ccgaattgaa ccggtgccta gagaaggtgg cgcggggtaa actgggaaag
     1921 tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc
     1981 agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc
     2041 cgtgtgtggt tcccgcgggc ctggcctctt tacgggttat ggcccttgcg tgccttgaat
     2101 tacttccacc tggctccagt acgtgattct tgatcccgag ctggagccag gggcgggcct
     2161 tgcgctttag gagccccttc gcctcgtgct tgagttgagg cctggcctgg gcgctggggc
     2221 cgccgcgtgc gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga taagtctcta
     2281 gccatttaaa atttttgatg acgtgctgcg acgctttttt tctggcaaga tagtcttgta
     2341 aatgcgggcc aggatctgca cactggtatt tcggtttttg ggcccgcggc cggcgacggg
     2401 gcccgtgcgt cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga
     2461 atcggacggg ggtagtctca agctggccgg cctgctctgg tgcctggcct cgcgccgccg
     2521 tgtatcgccc cgccctgggc ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa
     2581 agatggccgc ttcccggccc tgctccaggg ggctcaaaat ggaggacgcg gcgctcggga
     2641 gagcgggcgg gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc agccgtcgct
     2701 tcatgtgact ccacggagta ccgggcgccg tccaggcacc tcgattagtt ctggagcttt
     2761 tggagtacgt cgtctttagg ttggggggag gggttttatg cgatggagtt tccccacact
     2821 gagtgggtgg agactgaagt taggccagct tggcacttga tgtaattctc cttggaattt
     2881 ggcctttttg agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt
     2941 tttcttccat ttcaggtgtc gtgaacaacg cgtacgacca tgagcaaagg agaagaactt
     3001 ttcactggag ttgtcccaat tcttgttgaa ttagatggtg atgttaatgg gcacaaattt
     3061 tctgtccgtg gagagggtga aggtgatgct acaaacggaa aactcaccct taaatttatt
     3121 tgcactactg gaaaactacc tgttccgtgg ccaacacttg tcactactct gacctatggt
     3181 gttcaatgct tttcccgtta tccggatcac atgaaacggc atgacttttt caagagtgcc
     3241 atgcccgaag gttatgtaca ggaacgcact atatctttca aagatgacgg gacctacaag
     3301 acgcgtgctg aagtcaagtt tgaaggtgat acccttgtta atcgtatcga gttaaagggt
     3361 attgatttta aagaagatgg aaacattctt ggacacaaac tcgagtacaa ctttaactca
     3421 cacaatgtat acatcacggc agacaaacaa aagaatggaa tcaaagctaa cttcaaaatt
     3481 cgccacaacg ttgaagatgg ttccgttcaa ctagcagacc attatcaaca aaatactcca
     3541 attggcgatg gccctgtcct tttaccagac aaccattacc tgtcgacaca atctgtcctt
     3601 tcgaaagatc ccaacgaaaa gcgtgaccac atggtccttc ttgagtttgt aactgctgct
     3661 gggattacac atggcatgga tgagctctac aaaggcggtt ccgagggcag aggaagtctg
     3721 ctaacatgcg gtgacgtcga ggagaatcct ggcccaggcg gagggtcgat gagccggctg
     3781 gacaagagca aagtgatcaa cagcgccctg gaactgctga acggcgtggg catcgagggc
     3841 ctgaccaccc ggaagctggc ccagaaactg ggcgtggaac agcccaccct gtactggcac
     3901 gtgaagaaca agcgggccct gctggacgcc ctgcccatcg agatgctgga ccggcaccac
     3961 acccacagct gccctctgga aggcgagagc tggcaggact tcctgcggaa caacgccaag
     4021 agctacagat gcgccctgct gagccaccgg gacggcgcca aagtgcacct gggcaccaga
     4081 cccaccgaga agcagtacga gacactggaa aaccagctgg ccttcctgtg ccagcagggc
     4141 ttcagcctgg aaaacgccct gtacgccctg agcgccgtgg gccacttcac cctgggctgc
     4201 gtgctggaag aacaggaaca ccaggtcgcc aaagaggaaa gagagacacc caccaccgac
     4261 agcatgcccc ccctgctgaa gcaggccatc gagctgttcg acagacaggg cgccgagccc
     4321 gccttcctgt tcggcctgga actgatcatc tgcggcctcg agaagcagct gaagtgcgag
     4381 agcggcggac ccaccgacgc cctggacgac ttcgacctgg acatgctgcc cgccgatgcc
     4441 ctggatgatt ttgatctgga tatgctccct gctgacgctc tcgatgactt cgatctcgat
     4501 atgctgccag gctaagcggc cgcgactcta gagtcgacct gcaggcatgc aagcttgata
     4561 tcaagcttat cgataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     4621 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     4681 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
     4741 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
     4801 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
     4861 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     4921 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct
     4981 ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
     5041 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
     5101 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
     5161 cgcatcgata ccgtcgacct cgagggaatt aattcgagct cggtaccttt aagaccaatg
     5221 acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg
     5281 ctaattcact cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta
     5341 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     5401 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     5461 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagcat ctagaattaa
     5521 ttccgtgtat tctatagtgt cacctaaatc gtatgtgtat gatacataag gttatgtatt
     5581 aattgtagcc gcgttctaac gacaatatgt acaagcctaa ttgtgtagca tctggcttac
     5641 tgaagcagac cctatcatct ctctcgtaaa ctgccgtcag agtcggtttg gttggacgaa
     5701 ccttctgagt ttctggtaac gccgtcccgc acccggaaat ggtcagcgaa ccaatcagca
     5761 gggtcatcgc tagccagatc ctctacgccg gacgcatcgt ggccggcatc accggcgcca
     5821 caggtgcggt tgctggcgcc tatatcgccg acatcaccga tggggaagat cgggctcgcc
     5881 acttcgggct catgagcgct tgtttcggcg tgggtatggt ggcaggcccc gtggccgggg
     5941 gactgttggg cgccatctcc ttgcatgcac cattccttgc ggcggcggtg ctcaacggcc
     6001 tcaacctact actgggctgc ttcctaatgc aggagtcgca taagggagag cgtcgaatgg
     6061 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca
     6121 acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct
     6181 gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg
     6241 agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
     6301 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     6361 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     6421 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     6481 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     6541 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     6601 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     6661 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     6721 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     6781 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     6841 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     6901 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     6961 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     7021 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     7081 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     7141 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     7201 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     7261 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     7321 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     7381 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     7441 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     7501 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     7561 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     7621 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     7681 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     7741 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     7801 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     7861 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     7921 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     7981 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     8041 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     8101 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     8161 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     8221 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
     8281 ccgattcatt aatgcagctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct
     8341 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa
     8401 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     8461 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
     8521 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct
     8581 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc
     8641 ttggacacaa gacaggcttg cgagatatgt ttgagaatac cactttatcc cgcgtcaggg
     8701 agaggcagtg cgtaaaaaga cgcggactca tgtgaaatac tggtttttag tgcgccagat
     8761 ctctataatc tcgcgcaacc tattttcccc tcgaacactt tttaagccgt agataaacag
     8821 gctgggacac ttcacatgag cgaaaaatac atcgtcacct gggacatgtt gcagatccat
     8881 gcacgtaaac tcgcaagccg actgatgcct tctgaacaat ggaaaggcat tattgccgta
     8941 agccgtggcg gtctgtaccg ggtgcgttac tggcgcgtga actgggtatt cgtcatgtcg
     9001 ataccgtttg tatttccagc tacgatcacg acaaccagcg cgagcttaaa gtgctgaaac
     9061 gcgcagaagg cgatggcgaa ggcttcatcg ttattgatga cctggtggat accggtggta
     9121 ctgcggttgc gattcgtgaa atgtatccaa aagcgcactt tgtcaccatc ttcgcaaaac
     9181 cggctggtcg tccgctggtt gatgactatg ttgttgatat cccgcaagat acctggattg
     9241 aacagccgtg ggatatgggc gtcgtattcg tcccgccaat ctccggtcgc taatcttttc
     9301 aacgcctggc actgccgggc gttgttcttt ttaacttcag gcgggttaca atagtttcca
     9361 gtaagtattc tggaggctgc atccatgaca caggcaaacc tgagcgaaac cctgttcaaa
     9421 ccccgcttta aacatcctga aacctcgacg ctagtccgcc gctttaatca cggcgcacaa
     9481 ccgcctgtgc agtcggccct tgatggtaaa accatccctc actggtatcg catgattaac
     9541 cgtctgatgt ggatctggcg cggcattgac ccacgcgaaa tcctcgacgt ccaggcacgt
     9601 attgtgatga gcgatgccga acgtaccgac gatgatttat acgatacggt gattggctac
     9661 cgtggcggca actggattta tgagtgggcc ccggatcttt gtgaaggaac cttacttctg
     9721 tggtgtgaca taattggaca aactacctac agagatttaa agctctaagg taaatataaa
     9781 atttttaagt gtataatgtg ttaaactact gattctaatt gtttgtgtat tttagattcc
     9841 aacctatgga actgatgaat gggagcagtg gtggaatgcc tttaatgagg aaaacctgtt
     9901 ttgctcagaa gaaatgccat ctagtgatga tgaggctact gctgactctc aacattctac
     9961 tcctccaaaa aagaagagaa aggtagaaga ccccaaggac tttccttcag aattgctaag
    10021 ttttttgagt catgctgtgt ttagtaatag aactcttgct tgctttgcta tttacaccac
    10081 aaaggaaaaa gctgcactgc tatacaagaa aattatggaa aaatattctg taacctttat
    10141 aagtaggcat aacagttata atcataacat actgtttttt cttactccac acaggcatag
    10201 agtgtctgct attaataact atgctcaaaa attgtgtacc tttagctttt taatttgtaa
    10261 aggggttaat aaggaatatt tgatgtatag tgccttgact agagatcata atcagccata
    10321 ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga
    10381 aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca
    10441 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
    10501 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatcaactgg ataactcaag
    10561 ctaaccaaaa tcatcccaaa cttcccaccc cataccctat taccactgcc aattacctgt
    10621 ggtttcattt actctaaacc tgtgattcct ctgaattatt ttcattttaa agaaattgta
    10681 tttgttaaat atgtactaca aacttagtag ttggaagggc taattcactc ccaaagaaga
    10741 caagatatcc ttgatctgtg gatctaccac acacaaggct acttccctga ttagcagaac
    10801 tacacaccag ggccaggggt cagatatcca ctgacctttg gatggtgcta caagctagta
    10861 ccagttgagc cagataaggt agaagaggcc aataaaggag agaacaccag cttgttacac
    10921 cctgtgagcc tgcatgggat ggatgacccg gagagagaag tgttagagtg gaggtttgac
    10981 agccgcctag catttcatca cgtggcccga gagctgcatc cggagtactt caagaactgc
    11041 tgatatcgag cttgctacaa gggactttcc gctggggact ttccagggag gcgtggcctg
    11101 ggcgggactg gggagtggcg agccctcaga tcctgcatat aagcagctgc tttttgcctg
    11161 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
    11221 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
    11281 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
    11341 tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga
    11401 cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt
    11461 acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt
    11521 attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag
    11581 aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt
    11641 aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca
    11701 tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc aaccctctat
    11761 tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa
    11821 gagcaaaaca aaagtaagac caccgcacag caagcggccg gccgctgatc ttcagacctg
    11881 gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa
    11941 ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa
    12001 gagcagtggg aat
//
