LOCUS       sequence-296744-        5468 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  His-tagged CsoS2 C-terminal domain (CTD), pET-14b backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5468)
  AUTHORS   Oltrogge LM, Chaijarasphong T, Chen AW, Bolin ER, Marqusee S, Savage
            DF
  TITLE     Multivalent interactions between CsoS2 and Rubisco mediate 
            alpha-carboxysome formation.
  JOURNAL   Nat Struct Mol Biol. 2020 Mar;27(3):281-287. doi: 
            10.1038/s41594-020-0387-7. Epub 2020 Mar 2.
  PUBMED    32123388
REFERENCE   2  (bases 1 to 5468)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41594-020-0387-7"; journalName: "Nat Struct Mol Biol"; 
            date: "2020-03"; volume: "27"; issue: "3"; pages: "281-287"
COMMENT     Sequence Label: pBz110_CsoS2_CTD
FEATURES             Location/Qualifiers
     source          1..5468
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     5..24
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5..23
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             55..77
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             90..107
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(1002..1020)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      1016..1063
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(1429..1434,1435..1451,1452..1457))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(1484..1502)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1570..1674
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1675..1743,1744..2535)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1893..1912)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2706..3294
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3195..3214
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3448..3465
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    3480..3619
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(3545..3564)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3705..3727
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(3721..3912)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     5370..5389
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 aaattaatac gactcactat agggacacca caacggtttc cctctagaaa taattttgtt
       61 taactttaag aaggagatat accatgtccc accatcatca tcatcatacg agcaccccag
      121 agcccgaagc tcaatcaact gaacaatcat tgacctgtga aggacaaatt attagcggca
      181 cttcagttga cgccagtgat ttggtcacag gaaatgaaat cggtgaacag caactcatca
      241 gcggtgacgc ctatgttggc gcgcagcaga caggttgcct tcccactagt ccacgcttca
      301 accaaactgg caatgttcag tcaatgggtt ttaagaacac caatcagcca gaacaaaact
      361 ttgcaccagg tgaagtaatg cctactgact ttagtattca aaccccagct cgctcggctc
      421 agaatcgcat tacaggtaac gacattgcgc cctcaggtcg cattacaggc cctggtatgc
      481 tggcaaccgg cttgattaca ggaacccccg aattcaggca cgctgcgcgc gagttggttg
      541 gttctccaca acccatggca atggccatgg ccaaccgtaa taaggctgct caagcacctg
      601 ttgtgcagcc agaagtggtt gcaactcagg aaaagcctga gttggtatgt gcaccaagaa
      661 gcgatcaaat ggatcgtgtg agtggcgaag gcaaagaacg ttgccacatc actggcgatg
      721 actggtcagt taacaagcac atcaccggta cagccggtca atgggcgagt ggtcgcaacc
      781 cttccatgcg cggtaatgcg cgtgtggtcg aaaccagcgc gtttgccaat cgcaatgtgc
      841 caaaacctga aaagccgggc tccaagatca cgggcagtag tggtaatgac acccaaggta
      901 gtctgatcac ttactccggc ggcgcgcgcg gttgattaag taaagcggtt aagatccggc
      961 tgctaacaaa gcccgaaagg aagctgagtt ggctgccgcc accgctgagc aataactagc
     1021 ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat
     1081 atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa
     1141 gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg
     1201 gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga
     1261 tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta
     1321 tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca
     1381 tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat
     1441 cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta
     1501 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg
     1561 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     1621 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     1681 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     1741 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     1801 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     1861 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt
     1921 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     1981 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     2041 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     2101 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     2161 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca
     2221 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     2281 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     2341 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg ctctcgcggt
     2401 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     2461 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     2521 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     2581 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     2641 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     2701 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     2761 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     2821 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
     2881 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     2941 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     3001 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     3061 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     3121 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     3181 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     3241 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     3301 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     3361 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     3421 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     3481 ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcaa tggtgcactc
     3541 tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg
     3601 tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc
     3661 ttgtctgctc ccggcatccg cttacagaca agctgtgacc gcctccggga gctgcatgtg
     3721 tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc
     3781 gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt
     3841 ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc
     3901 ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc
     3961 gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact
     4021 ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac
     4081 tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca
     4141 gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag
     4201 actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt
     4261 gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag
     4321 gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg
     4381 ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat
     4441 ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc
     4501 tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag
     4561 gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg
     4621 cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg
     4681 atcagcggtc caatgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt
     4741 ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg
     4801 ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc
     4861 agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg
     4921 aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat
     4981 accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg
     5041 acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt
     5101 gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc
     5161 aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt
     5221 aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc
     5281 aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc
     5341 ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc
     5401 gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg
     5461 atcccgcg
//
