LOCUS       sequence-296751-       10665 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses fluorescently-tagged human Type I procollagen alpha-1 
            chain with mVenus replacing exon 2-3 retaining N-propeptide minor 
            triple helix and N-terminal cleavage site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10665)
  TITLE     Fluorescent protein-tagged human type I procollagen expression 
            vectors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10665)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: mVenus-hCOL1A1
FEATURES             Location/Qualifiers
     source          1..10665
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     844..863
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        844..862
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(871..904)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             join(1141..1143,1144..1851)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELY"
     primer_bind     complement(1183..1204)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1444..1463)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     complement(6256..6273)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6262..6486
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6532..6960
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6619..6638)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6829..6850
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6969..6989)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6974..7303
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7154..7289
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7216..7235
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             7370..8164
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7424..7443)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8034..8053
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8338..8459
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8375..8394)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8429..8448
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8508..8524)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8508..8524)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8521..8543)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8532..8548
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8556..8562,8563..8580,8581..8586))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8601..8622
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8739..8756)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8910..9498)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8990..9009)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9669..10460,10461..10529))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10292..10311
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10530..10634)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtccgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta cgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacaccaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta ataaccccgc cccgttgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagaatt
      841 ttgtaatacg actcactata gggcggccgc ataacttcgt atagcataca ttatacgaag
      901 ttatggatca ggccaaatcg gccgagctcg aattcgtcga gagcggagac gggagtttct
      961 cctcggggtc ggagcaggag gcacgcggag tgtgaggcca cgcatgagcg gacgctaacc
     1021 ccctccccag ccacaaagag tctacatgtc tagggtctag acatgttcag ctttgtggac
     1081 ctccggctcc tgctcctctt agcggccacc gccctcctga cgcacggcca agaggatatc
     1141 gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc
     1201 gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc
     1261 aagctgaccc tgaagctgat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc
     1321 gtgaccaccc tgggctacgg cctgcagtgc ttcgcccgct accccgacca catgaagcag
     1381 cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc
     1441 aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg
     1501 aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag
     1561 ctggagtaca actacaacag ccacaacgtc tatatcaccg ccgacaagca gaagaacggc
     1621 atcaaggcca acttcaagat ccgccacaac atcgaggacg gcggcgtgca gctcgccgac
     1681 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac
     1741 ctgagctacc agtccaagct gagcaaagac cccaacgaga agcgcgatca catggtcctg
     1801 ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta cgaccaagaa
     1861 accaccggcg tcgagggacc caagggagac actggccccc gaggcccaag gggacccgca
     1921 ggcccccctg gccgagatgg catccctgga cagcctggac ttcccggacc ccccggaccc
     1981 cccggacctc ccggaccccc tggcctcgga ggaaactttg ctccccagct gtcttatggc
     2041 tatgatgaga aatcaaccgg aggaatttcc gtgcctggcc ccatgggtcc ctctggtcct
     2101 cgtggtctcc ctggcccccc tggtgcacct ggtccccaag gcttccaagg tccccctggt
     2161 gagcctggcg agcctggagc ttcaggtccc atgggtcccc gaggtccccc aggtccccct
     2221 ggaaagaatg gagatgatgg ggaagctgga aaacctggtc gtcctggtga gcgtgggcct
     2281 cctgggcctc agggtgctcg aggattgccc ggaacagctg gcctccctgg aatgaaggga
     2341 cacagaggtt tcagtggttt ggatggtgcc aagggagatg ctggtcctgc tggtcctaag
     2401 ggtgagcctg gcagccctgg tgaaaatgga gctcctggtc agatgggccc ccgtggcctg
     2461 cctggtgaga gaggtcgccc tggagcccct ggccctgctg gtgctcgtgg aaatgatggt
     2521 gctactggtg ctgccgggcc ccctggtccc accggccccg ctggtcctcc tggcttccct
     2581 ggtgctgttg gtgctaaggg tgaagctggt ccccaagggc cccgaggctc tgaaggtccc
     2641 cagggtgtgc gtggtgagcc tggcccccct ggccctgctg gtgctgctgg ccctgctgga
     2701 aaccctggtg ctgatggaca gcctggtgct aaaggtgcca atggtgctcc tggtattgct
     2761 ggtgctcctg gcttccctgg tgcccgaggc ccctctggac cccagggccc cggcggccct
     2821 cctggtccca agggtaacag cggtgaacct ggtgctcctg gcagcaaagg agacactggt
     2881 gctaagggag agcctggccc tgttggtgtt caaggacccc ctggccctgc tggagaggaa
     2941 ggaaagcgag gagctcgagg tgaacccgga cccactggcc tgcccggacc ccctggcgag
     3001 cgtggtggac ctggtagccg tggtttccct ggcgcagatg gtgttgctgg tcccaagggt
     3061 cccgctggtg aacgtggttc tcctggccct gctggcccca aaggatctcc tggtgaagct
     3121 ggtcgtcccg gtgaagctgg tctgcctggt gccaagggtc tgactggaag ccctggcagc
     3181 cctggtcctg atggcaaaac tggcccccct ggtcccgccg gtcaagatgg tcgccccgga
     3241 cccccaggcc cacctggtgc ccgtggtcag gctggtgtga tgggattccc tggacctaaa
     3301 ggtgctgctg gagagcccgg caaggctgga gagcgaggtg ttcccggacc ccctggcgct
     3361 gtcggtcctg ctggcaaaga tggagaggct ggagctcagg gaccccctgg ccctgctggt
     3421 cccgctggcg agagaggtga acaaggccct gctggctccc ccggattcca gggtctccct
     3481 ggtcctgctg gtcctccagg tgaagcaggc aaacctggtg aacagggtgt tcctggagac
     3541 cttggcgccc ctggcccctc tggagcaaga ggcgagagag gtttccctgg cgagcgtggt
     3601 gtgcaaggtc cccctggtcc tgctggtccc cgaggggcca acggtgctcc cggcaacgat
     3661 ggtgctaagg gtgatgctgg tgcccctgga gctcccggta gccagggcgc ccctggcctt
     3721 cagggaatgc ctggtgaacg tggtgcagct ggtcttccag ggcctaaggg tgacagaggt
     3781 gatgctggtc ccaaaggtgc tgatggctct cctggcaaag atggcgtccg tggtctgacc
     3841 ggccccattg gtcctcctgg ccctgctggt gcccctggtg acaagggtga aagtggtccc
     3901 agcggccctg ctggtcccac tggagctcgt ggtgcccccg gagaccgtgg tgagcctggt
     3961 ccccccggcc ctgctggctt tgctggcccc cctggtgctg acggccaacc tggtgctaaa
     4021 ggcgaacctg gtgatgctgg tgctaaaggc gatgctggtc cccctggccc tgccggaccc
     4081 gctggacccc ctggccccat tggtaatgtt ggtgctcctg gagccaaagg tgctcgcggc
     4141 agcgctggtc cccctggtgc tactggtttc cctggtgctg ctggccgagt cggtcctcct
     4201 ggcccctctg gaaatgctgg accccctggc cctcctggtc ctgctggcaa agaaggcggc
     4261 aaaggtcccc gtggtgagac tggccctgct ggacgtcctg gtgaagttgg tccccctggt
     4321 ccccctggcc ctgctggcga gaaaggatcc cctggtgctg atggtcctgc tggtgctcct
     4381 ggtactcccg ggcctcaagg tattgctgga cagcgtggtg tggtcggcct gcctggtcag
     4441 agaggagaga gaggcttccc tggtcttcct ggcccctctg gtgaacctgg caaacaaggt
     4501 ccctctggag caagtggtga acgtggtccc cctggtccca tgggcccccc tggattggct
     4561 ggaccccctg gtgaatctgg acgtgagggg gctcctggtg ccgaaggttc ccctggacga
     4621 gacggttctc ctggcgccaa gggtgaccgt ggtgagaccg gccccgctgg accccctggt
     4681 gctcctggtg ctcctggtgc ccctggcccc gttggccctg ctggcaagag tggtgatcgt
     4741 ggtgagactg gtcctgctgg tcccgccggt cctgtcggcc ctgttggcgc ccgtggcccc
     4801 gccggacccc aaggcccacg tggtgacaag ggtgagacag gcgaacaggg cgacagaggc
     4861 ataaagggtc accgtggctt ctctggcctc cagggtcccc ctggccctcc tggctctcct
     4921 ggtgaacaag gtccctctgg agcctctggt cctgctggtc cccgaggtcc ccctggctct
     4981 gctggtgctc ctggcaaaga tggactcaac ggtctccctg gccccattgg gccccctggt
     5041 cctcgcggtc gcactggtga tgctggtcct gttggtcccc ccggccctcc tggacctcct
     5101 ggtccccctg gtcctcccag cgctggtttc gacttcagct tcctgcccca gccacctcaa
     5161 gagaaggctc acgatggtgg ccgctactac cgggctgatg atgccaatgt ggttcgtgac
     5221 cgtgacctcg aggtggacac caccctcaag agcctgagcc agcagatcga gaacatccgg
     5281 agcccagagg gcagccgcaa gaaccccgcc cgcacctgcc gtgacctcaa gatgtgccac
     5341 tctgactgga agagtggaga gtactggatt gaccccaacc aaggctgcaa cctggatgcc
     5401 atcaaagtct tctgcaacat ggagactggt gagacctgcg tgtaccccac tcagcccagt
     5461 gtggcccaga agaactggta catcagcaag aaccccaagg acaagaggca tgtctggttc
     5521 ggcgagagca tgaccgatgg attccagttc gagtatggcg gccagggctc cgaccctgcc
     5581 gatgtggcca tccagctgac cttcctgcgc ctgatgtcca ccgaggcctc ccagaacatc
     5641 acctaccact gcaagaacag cgtggcctac atggaccagc agactggcaa cctcaagaag
     5701 gccctgctcc tccagggctc caacgagatc gagatccgcg ccgagggcaa cagccgcttc
     5761 acctacagcg tcactgtcga tggctgcacg agtcacaccg gagcctgggg caagacagtg
     5821 attgaataca aaaccaccaa gacctcccgc ctgcgcatca tcgatgtggc ccccttggac
     5881 gttggtgccc cagaccagga attcggcttc gacgttggcc atgtctgctt cctgtaaact
     5941 ccctccatcc caacctggct ccctcccacc caaccaactt tccccccaac ccggaaacag
     6001 acaagcaacc caaactgaac cccctcaaaa gccaaaaaat gggagacaat ttcacatgga
     6061 ctttggaaaa tatttttttc ctttgcattc atctctcaaa cttagttttt atctttgacc
     6121 aaccgaacat gaccaaaaac caaaagtgca ttcaacctta ccaaaaaaaa aaaaaaaact
     6181 ctccagcgct ggatcccctg gtgctgatgg tcctgctggt gctcctggta ctccccctag
     6241 aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct
     6301 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg
     6361 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc
     6421 aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct
     6481 ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct
     6541 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     6601 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     6661 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     6721 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
     6781 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     6841 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
     6901 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     6961 aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag
     7021 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
     7081 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
     7141 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     7201 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca
     7261 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg
     7321 tatatccatt ttcggatctg atcaagagac aggatgagga tcgtttcgca tgattgaaca
     7381 agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg
     7441 ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg
     7501 cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc
     7561 agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt
     7621 cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc
     7681 atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca
     7741 tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc
     7801 acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg
     7861 gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgaggatct
     7921 cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc
     7981 tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc
     8041 tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta
     8101 cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt
     8161 ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga
     8221 gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac
     8281 gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac
     8341 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     8401 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     8461 catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt
     8521 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     8581 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     8641 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     8701 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     8761 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     8821 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     8881 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     8941 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     9001 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     9061 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     9121 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     9181 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     9241 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     9301 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     9361 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     9421 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     9481 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     9541 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     9601 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     9661 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     9721 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     9781 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     9841 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     9901 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     9961 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
    10021 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
    10081 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
    10141 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
    10201 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
    10261 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
    10321 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
    10381 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
    10441 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
    10501 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
    10561 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
    10621 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtc
//
