LOCUS       sequence-296772-       10656 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses fluorescently-tagged human Type I procollagen alpha-1 
            chain with mApple replacing exon 2-3 retaining N-propeptide minor 
            triple helix and N-terminal cleavage site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10656)
  TITLE     Fluorescent protein-tagged human type I procollagen expression 
            vectors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10656)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: mApple-hCOL1A1
FEATURES             Location/Qualifiers
     source          1..10656
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(88..105)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    94..318
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      364..792
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(451..470)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     661..682
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(801..821)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        806..1135
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      986..1121
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     1048..1067
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             1202..1996
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1256..1275)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1866..1885
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    2170..2291
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2207..2226)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2261..2280
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2340..2356)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2340..2356)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2353..2375)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2364..2380
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2388..2394,2395..2412,2413..2418))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2433..2454
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2571..2588)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2742..3330)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2822..2841)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3501..4292,4293..4361))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4124..4143
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4362..4466)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(4541..4560)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        4732..5111
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5112..5315
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5266..5286
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5341..5360
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5341..5359
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(5368..5401)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             5638..6339
                     /codon_start=1
                     /product="photostable monomeric derivative of DsRed (Shaner
                     et al., 2008)"
                     /label=mApple
                     /note="mammalian codon-optimized"
                     /translation="VSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEAF
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNFE
                     DGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALKS
                     EIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERAE
                     GRHSTGGMDELY"
     primer_bind     6048..6067
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 aaaaaaaaaa ctctccagcg ctggatcccc tggtgctgat ggtcctgctg gtgctcctgg
       61 tactccccct agaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg
      121 ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt
      181 cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg
      241 gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg
      301 atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc
      361 cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
      421 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
      481 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
      541 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
      601 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
      661 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
      721 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
      781 ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
      841 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
      901 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
      961 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     1021 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc
     1081 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
     1141 cccgggagct tgtatatcca ttttcggatc tgatcaagag acaggatgag gatcgtttcg
     1201 catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt
     1261 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc
     1321 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact
     1381 gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt
     1441 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca
     1501 ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat
     1561 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg
     1621 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga
     1681 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga
     1741 cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa
     1801 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga
     1861 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt
     1921 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct
     1981 tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac
     2041 ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc
     2101 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc
     2161 gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     2221 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     2281 aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg
     2341 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     2401 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     2461 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     2521 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     2581 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     2641 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     2701 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     2761 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     2821 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     2881 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     2941 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     3001 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     3061 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     3121 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     3181 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     3241 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     3301 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     3361 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     3421 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     3481 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     3541 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     3601 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     3661 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     3721 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     3781 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     3841 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     3901 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     3961 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     4021 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     4081 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     4141 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     4201 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     4261 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     4321 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     4381 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     4441 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac
     4501 ggatcgggag atctcccgat cccctatggt gcactctcag tacaatctgc tctgatgccg
     4561 catagttaag ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga
     4621 gcaaaattta agctacaaca aggcaaggct tgaccgacaa ttgcatgaag aatctgctta
     4681 gggttaggcg ttttgcgctg cttcgcgatg tacgggccag atatacgcgt tgacattgat
     4741 tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg
     4801 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
     4861 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
     4921 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
     4981 atatgccaag tccgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
     5041 cccagtacat gaccttacgg gactttccta cttggcagta catctacgta ttagtcatcg
     5101 ctattaccat ggtgatgcgg ttttggcagt acaccaatgg gcgtggatag cggtttgact
     5161 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
     5221 atcaacggga ctttccaaaa tgtcgtaata accccgcccc gttgacgcaa atgggcggta
     5281 ggcgtgtacg gtgggaggtc tatataagca gagctcgttt agtgaaccgt cagaattttg
     5341 taatacgact cactataggg cggccgcata acttcgtata gcatacatta tacgaagtta
     5401 tggatcaggc caaatcggcc gagctcgaat tcgtcgagag cggagacggg agtttctcct
     5461 cggggtcgga gcaggaggca cgcggagtgt gaggccacgc atgagcggac gctaaccccc
     5521 tccccagcca caaagagtct acatgtctag ggtctagaca tgttcagctt tgtggacctc
     5581 cggctcctgc tcctcttagc ggccaccgcc ctcctgacgc acggccaaga ggatatcgtg
     5641 agcaagggcg aggagaataa catggccatc atcaaggagt tcatgcgctt caaggtgcac
     5701 atggagggct ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc
     5761 tacgaggcct ttcagaccgc taagctgaag gtgaccaagg gtggccccct gcccttcgcc
     5821 tgggacatcc tgtcccctca gttcatgtac ggctccaagg tctacattaa gcacccagcc
     5881 gacatccccg actacttcaa gctgtccttc cccgagggct tcaggtggga gcgcgtgatg
     5941 aacttcgagg acggcggcat tattcacgtt aaccaggact cctccctgca ggacggcgtg
     6001 ttcatctaca aggtgaagct gcgcggcacc aacttcccct ccgacggccc cgtaatgcag
     6061 aagaagacca tgggctggga ggcctccgag gagcggatgt accccgagga cggcgccctg
     6121 aagagcgaga tcaagaagag gctgaagctg aaggacggcg gccactacgc cgccgaggtc
     6181 aagaccacct acaaggccaa gaagcccgtg cagctgcccg gcgcctacat cgtcgacatc
     6241 aagttggaca tcgtgtccca caacgaggac tacaccatcg tggaacagta cgaacgcgcc
     6301 gagggccgcc actccaccgg cggcatggac gagctgtacg accaagaaac caccggcgtc
     6361 gagggaccca agggagacac tggcccccga ggcccaaggg gacccgcagg cccccctggc
     6421 cgagatggca tccctggaca gcctggactt cccggacccc ccggaccccc cggacctccc
     6481 ggaccccctg gcctcggagg aaactttgct ccccagctgt cttatggcta tgatgagaaa
     6541 tcaaccggag gaatttccgt gcctggcccc atgggtccct ctggtcctcg tggtctccct
     6601 ggcccccctg gtgcacctgg tccccaaggc ttccaaggtc cccctggtga gcctggcgag
     6661 cctggagctt caggtcccat gggtccccga ggtcccccag gtccccctgg aaagaatgga
     6721 gatgatgggg aagctggaaa acctggtcgt cctggtgagc gtgggcctcc tgggcctcag
     6781 ggtgctcgag gattgcccgg aacagctggc ctccctggaa tgaagggaca cagaggtttc
     6841 agtggtttgg atggtgccaa gggagatgct ggtcctgctg gtcctaaggg tgagcctggc
     6901 agccctggtg aaaatggagc tcctggtcag atgggccccc gtggcctgcc tggtgagaga
     6961 ggtcgccctg gagcccctgg ccctgctggt gctcgtggaa atgatggtgc tactggtgct
     7021 gccgggcccc ctggtcccac cggccccgct ggtcctcctg gcttccctgg tgctgttggt
     7081 gctaagggtg aagctggtcc ccaagggccc cgaggctctg aaggtcccca gggtgtgcgt
     7141 ggtgagcctg gcccccctgg ccctgctggt gctgctggcc ctgctggaaa ccctggtgct
     7201 gatggacagc ctggtgctaa aggtgccaat ggtgctcctg gtattgctgg tgctcctggc
     7261 ttccctggtg cccgaggccc ctctggaccc cagggccccg gcggccctcc tggtcccaag
     7321 ggtaacagcg gtgaacctgg tgctcctggc agcaaaggag acactggtgc taagggagag
     7381 cctggccctg ttggtgttca aggaccccct ggccctgctg gagaggaagg aaagcgagga
     7441 gctcgaggtg aacccggacc cactggcctg cccggacccc ctggcgagcg tggtggacct
     7501 ggtagccgtg gtttccctgg cgcagatggt gttgctggtc ccaagggtcc cgctggtgaa
     7561 cgtggttctc ctggccctgc tggccccaaa ggatctcctg gtgaagctgg tcgtcccggt
     7621 gaagctggtc tgcctggtgc caagggtctg actggaagcc ctggcagccc tggtcctgat
     7681 ggcaaaactg gcccccctgg tcccgccggt caagatggtc gccccggacc cccaggccca
     7741 cctggtgccc gtggtcaggc tggtgtgatg ggattccctg gacctaaagg tgctgctgga
     7801 gagcccggca aggctggaga gcgaggtgtt cccggacccc ctggcgctgt cggtcctgct
     7861 ggcaaagatg gagaggctgg agctcaggga ccccctggcc ctgctggtcc cgctggcgag
     7921 agaggtgaac aaggccctgc tggctccccc ggattccagg gtctccctgg tcctgctggt
     7981 cctccaggtg aagcaggcaa acctggtgaa cagggtgttc ctggagacct tggcgcccct
     8041 ggcccctctg gagcaagagg cgagagaggt ttccctggcg agcgtggtgt gcaaggtccc
     8101 cctggtcctg ctggtccccg aggggccaac ggtgctcccg gcaacgatgg tgctaagggt
     8161 gatgctggtg cccctggagc tcccggtagc cagggcgccc ctggccttca gggaatgcct
     8221 ggtgaacgtg gtgcagctgg tcttccaggg cctaagggtg acagaggtga tgctggtccc
     8281 aaaggtgctg atggctctcc tggcaaagat ggcgtccgtg gtctgaccgg ccccattggt
     8341 cctcctggcc ctgctggtgc ccctggtgac aagggtgaaa gtggtcccag cggccctgct
     8401 ggtcccactg gagctcgtgg tgcccccgga gaccgtggtg agcctggtcc ccccggccct
     8461 gctggctttg ctggcccccc tggtgctgac ggccaacctg gtgctaaagg cgaacctggt
     8521 gatgctggtg ctaaaggcga tgctggtccc cctggccctg ccggacccgc tggaccccct
     8581 ggccccattg gtaatgttgg tgctcctgga gccaaaggtg ctcgcggcag cgctggtccc
     8641 cctggtgcta ctggtttccc tggtgctgct ggccgagtcg gtcctcctgg cccctctgga
     8701 aatgctggac cccctggccc tcctggtcct gctggcaaag aaggcggcaa aggtccccgt
     8761 ggtgagactg gccctgctgg acgtcctggt gaagttggtc cccctggtcc ccctggccct
     8821 gctggcgaga aaggatcccc tggtgctgat ggtcctgctg gtgctcctgg tactcccggg
     8881 cctcaaggta ttgctggaca gcgtggtgtg gtcggcctgc ctggtcagag aggagagaga
     8941 ggcttccctg gtcttcctgg cccctctggt gaacctggca aacaaggtcc ctctggagca
     9001 agtggtgaac gtggtccccc tggtcccatg ggcccccctg gattggctgg accccctggt
     9061 gaatctggac gtgagggggc tcctggtgcc gaaggttccc ctggacgaga cggttctcct
     9121 ggcgccaagg gtgaccgtgg tgagaccggc cccgctggac cccctggtgc tcctggtgct
     9181 cctggtgccc ctggccccgt tggccctgct ggcaagagtg gtgatcgtgg tgagactggt
     9241 cctgctggtc ccgccggtcc tgtcggccct gttggcgccc gtggccccgc cggaccccaa
     9301 ggcccacgtg gtgacaaggg tgagacaggc gaacagggcg acagaggcat aaagggtcac
     9361 cgtggcttct ctggcctcca gggtccccct ggccctcctg gctctcctgg tgaacaaggt
     9421 ccctctggag cctctggtcc tgctggtccc cgaggtcccc ctggctctgc tggtgctcct
     9481 ggcaaagatg gactcaacgg tctccctggc cccattgggc cccctggtcc tcgcggtcgc
     9541 actggtgatg ctggtcctgt tggtcccccc ggccctcctg gacctcctgg tccccctggt
     9601 cctcccagcg ctggtttcga cttcagcttc ctgccccagc cacctcaaga gaaggctcac
     9661 gatggtggcc gctactaccg ggctgatgat gccaatgtgg ttcgtgaccg tgacctcgag
     9721 gtggacacca ccctcaagag cctgagccag cagatcgaga acatccggag cccagagggc
     9781 agccgcaaga accccgcccg cacctgccgt gacctcaaga tgtgccactc tgactggaag
     9841 agtggagagt actggattga ccccaaccaa ggctgcaacc tggatgccat caaagtcttc
     9901 tgcaacatgg agactggtga gacctgcgtg taccccactc agcccagtgt ggcccagaag
     9961 aactggtaca tcagcaagaa ccccaaggac aagaggcatg tctggttcgg cgagagcatg
    10021 accgatggat tccagttcga gtatggcggc cagggctccg accctgccga tgtggccatc
    10081 cagctgacct tcctgcgcct gatgtccacc gaggcctccc agaacatcac ctaccactgc
    10141 aagaacagcg tggcctacat ggaccagcag actggcaacc tcaagaaggc cctgctcctc
    10201 cagggctcca acgagatcga gatccgcgcc gagggcaaca gccgcttcac ctacagcgtc
    10261 actgtcgatg gctgcacgag tcacaccgga gcctggggca agacagtgat tgaatacaaa
    10321 accaccaaga cctcccgcct gcgcatcatc gatgtggccc ccttggacgt tggtgcccca
    10381 gaccaggaat tcggcttcga cgttggccat gtctgcttcc tgtaaactcc ctccatccca
    10441 acctggctcc ctcccaccca accaactttc cccccaaccc ggaaacagac aagcaaccca
    10501 aactgaaccc cctcaaaagc caaaaaatgg gagacaattt cacatggact ttggaaaata
    10561 tttttttcct ttgcattcat ctctcaaact tagtttttat ctttgaccaa ccgaacatga
    10621 ccaaaaacca aaagtgcatt caaccttacc aaaaaa
//
