LOCUS       sequence-296778-       10664 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses fluorescently-tagged human Type I procollagen alpha-1 
            chain with mCerulean replacing exon 2-3 retaining N-propeptide minor
            triple helix and N-terminal cleavage site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10664)
  TITLE     Fluorescent protein-tagged human type I procollagen expression 
            vectors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10664)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: mCerulean-hCOL1A1
FEATURES             Location/Qualifiers
     source          1..10664
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     844..863
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        844..862
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(871..904)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             join(1141..1143,1144..1851)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIKA
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELY"
     primer_bind     complement(1183..1204)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1444..1463)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     complement(6255..6272)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6261..6485
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6531..6959
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6618..6637)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6828..6849
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6968..6988)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6973..7302
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7153..7288
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7215..7234
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             7369..8163
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7423..7442)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8033..8052
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8337..8458
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8374..8393)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8428..8447
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8507..8523)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8507..8523)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8520..8542)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8531..8547
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8555..8561,8562..8579,8580..8585))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8600..8621
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8738..8755)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8909..9497)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8989..9008)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9668..10459,10460..10528))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10291..10310
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10529..10633)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtccgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta cgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacaccaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta ataaccccgc cccgttgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagaatt
      841 ttgtaatacg actcactata gggcggccgc ataacttcgt atagcataca ttatacgaag
      901 ttatggatca ggccaaatcg gccgagctcg aattcgtcga gagcggagac gggagtttct
      961 cctcggggtc ggagcaggag gcacgcggag tgtgaggcca cgcatgagcg gacgctaacc
     1021 ccctccccag ccacaaagag tctacatgtc tagggtctag acatgttcag ctttgtggac
     1081 ctccggctcc tgctcctctt agcggccacc gccctcctga cgcacggcca agaggatatc
     1141 gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc
     1201 gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc
     1261 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc
     1321 gtgaccaccc tgacctgggg cgtgcagtgc ttcgcccgct accccgacca catgaagcag
     1381 cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc
     1441 aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg
     1501 aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag
     1561 ctggagtaca acgccatcag cgacaacgtc tatatcaccg ccgacaagca gaagaacggc
     1621 atcaaggcca acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac
     1681 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac
     1741 ctgagcaccc agtccaagct gagcaaagac cccaacgaga agcgcgatca catggtcctg
     1801 ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta caccaagaaa
     1861 ccaccggcgt cgagggaccc aagggagaca ctggcccccg aggcccaagg ggacccgcag
     1921 gcccccctgg ccgagatggc atccctggac agcctggact tcccggaccc cccggacccc
     1981 ccggacctcc cggaccccct ggcctcggag gaaactttgc tccccagctg tcttatggct
     2041 atgatgagaa atcaaccgga ggaatttccg tgcctggccc catgggtccc tctggtcctc
     2101 gtggtctccc tggcccccct ggtgcacctg gtccccaagg cttccaaggt ccccctggtg
     2161 agcctggcga gcctggagct tcaggtccca tgggtccccg aggtccccca ggtccccctg
     2221 gaaagaatgg agatgatggg gaagctggaa aacctggtcg tcctggtgag cgtgggcctc
     2281 ctgggcctca gggtgctcga ggattgcccg gaacagctgg cctccctgga atgaagggac
     2341 acagaggttt cagtggtttg gatggtgcca agggagatgc tggtcctgct ggtcctaagg
     2401 gtgagcctgg cagccctggt gaaaatggag ctcctggtca gatgggcccc cgtggcctgc
     2461 ctggtgagag aggtcgccct ggagcccctg gccctgctgg tgctcgtgga aatgatggtg
     2521 ctactggtgc tgccgggccc cctggtccca ccggccccgc tggtcctcct ggcttccctg
     2581 gtgctgttgg tgctaagggt gaagctggtc cccaagggcc ccgaggctct gaaggtcccc
     2641 agggtgtgcg tggtgagcct ggcccccctg gccctgctgg tgctgctggc cctgctggaa
     2701 accctggtgc tgatggacag cctggtgcta aaggtgccaa tggtgctcct ggtattgctg
     2761 gtgctcctgg cttccctggt gcccgaggcc cctctggacc ccagggcccc ggcggccctc
     2821 ctggtcccaa gggtaacagc ggtgaacctg gtgctcctgg cagcaaagga gacactggtg
     2881 ctaagggaga gcctggccct gttggtgttc aaggaccccc tggccctgct ggagaggaag
     2941 gaaagcgagg agctcgaggt gaacccggac ccactggcct gcccggaccc cctggcgagc
     3001 gtggtggacc tggtagccgt ggtttccctg gcgcagatgg tgttgctggt cccaagggtc
     3061 ccgctggtga acgtggttct cctggccctg ctggccccaa aggatctcct ggtgaagctg
     3121 gtcgtcccgg tgaagctggt ctgcctggtg ccaagggtct gactggaagc cctggcagcc
     3181 ctggtcctga tggcaaaact ggcccccctg gtcccgccgg tcaagatggt cgccccggac
     3241 ccccaggccc acctggtgcc cgtggtcagg ctggtgtgat gggattccct ggacctaaag
     3301 gtgctgctgg agagcccggc aaggctggag agcgaggtgt tcccggaccc cctggcgctg
     3361 tcggtcctgc tggcaaagat ggagaggctg gagctcaggg accccctggc cctgctggtc
     3421 ccgctggcga gagaggtgaa caaggccctg ctggctcccc cggattccag ggtctccctg
     3481 gtcctgctgg tcctccaggt gaagcaggca aacctggtga acagggtgtt cctggagacc
     3541 ttggcgcccc tggcccctct ggagcaagag gcgagagagg tttccctggc gagcgtggtg
     3601 tgcaaggtcc ccctggtcct gctggtcccc gaggggccaa cggtgctccc ggcaacgatg
     3661 gtgctaaggg tgatgctggt gcccctggag ctcccggtag ccagggcgcc cctggccttc
     3721 agggaatgcc tggtgaacgt ggtgcagctg gtcttccagg gcctaagggt gacagaggtg
     3781 atgctggtcc caaaggtgct gatggctctc ctggcaaaga tggcgtccgt ggtctgaccg
     3841 gccccattgg tcctcctggc cctgctggtg cccctggtga caagggtgaa agtggtccca
     3901 gcggccctgc tggtcccact ggagctcgtg gtgcccccgg agaccgtggt gagcctggtc
     3961 cccccggccc tgctggcttt gctggccccc ctggtgctga cggccaacct ggtgctaaag
     4021 gcgaacctgg tgatgctggt gctaaaggcg atgctggtcc ccctggccct gccggacccg
     4081 ctggaccccc tggccccatt ggtaatgttg gtgctcctgg agccaaaggt gctcgcggca
     4141 gcgctggtcc ccctggtgct actggtttcc ctggtgctgc tggccgagtc ggtcctcctg
     4201 gcccctctgg aaatgctgga ccccctggcc ctcctggtcc tgctggcaaa gaaggcggca
     4261 aaggtccccg tggtgagact ggccctgctg gacgtcctgg tgaagttggt ccccctggtc
     4321 cccctggccc tgctggcgag aaaggatccc ctggtgctga tggtcctgct ggtgctcctg
     4381 gtactcccgg gcctcaaggt attgctggac agcgtggtgt ggtcggcctg cctggtcaga
     4441 gaggagagag aggcttccct ggtcttcctg gcccctctgg tgaacctggc aaacaaggtc
     4501 cctctggagc aagtggtgaa cgtggtcccc ctggtcccat gggcccccct ggattggctg
     4561 gaccccctgg tgaatctgga cgtgaggggg ctcctggtgc cgaaggttcc cctggacgag
     4621 acggttctcc tggcgccaag ggtgaccgtg gtgagaccgg ccccgctgga ccccctggtg
     4681 ctcctggtgc tcctggtgcc cctggccccg ttggccctgc tggcaagagt ggtgatcgtg
     4741 gtgagactgg tcctgctggt cccgccggtc ctgtcggccc tgttggcgcc cgtggccccg
     4801 ccggacccca aggcccacgt ggtgacaagg gtgagacagg cgaacagggc gacagaggca
     4861 taaagggtca ccgtggcttc tctggcctcc agggtccccc tggccctcct ggctctcctg
     4921 gtgaacaagg tccctctgga gcctctggtc ctgctggtcc ccgaggtccc cctggctctg
     4981 ctggtgctcc tggcaaagat ggactcaacg gtctccctgg ccccattggg ccccctggtc
     5041 ctcgcggtcg cactggtgat gctggtcctg ttggtccccc cggccctcct ggacctcctg
     5101 gtccccctgg tcctcccagc gctggtttcg acttcagctt cctgccccag ccacctcaag
     5161 agaaggctca cgatggtggc cgctactacc gggctgatga tgccaatgtg gttcgtgacc
     5221 gtgacctcga ggtggacacc accctcaaga gcctgagcca gcagatcgag aacatccgga
     5281 gcccagaggg cagccgcaag aaccccgccc gcacctgccg tgacctcaag atgtgccact
     5341 ctgactggaa gagtggagag tactggattg accccaacca aggctgcaac ctggatgcca
     5401 tcaaagtctt ctgcaacatg gagactggtg agacctgcgt gtaccccact cagcccagtg
     5461 tggcccagaa gaactggtac atcagcaaga accccaagga caagaggcat gtctggttcg
     5521 gcgagagcat gaccgatgga ttccagttcg agtatggcgg ccagggctcc gaccctgccg
     5581 atgtggccat ccagctgacc ttcctgcgcc tgatgtccac cgaggcctcc cagaacatca
     5641 cctaccactg caagaacagc gtggcctaca tggaccagca gactggcaac ctcaagaagg
     5701 ccctgctcct ccagggctcc aacgagatcg agatccgcgc cgagggcaac agccgcttca
     5761 cctacagcgt cactgtcgat ggctgcacga gtcacaccgg agcctggggc aagacagtga
     5821 ttgaatacaa aaccaccaag acctcccgcc tgcgcatcat cgatgtggcc cccttggacg
     5881 ttggtgcccc agaccaggaa ttcggcttcg acgttggcca tgtctgcttc ctgtaaactc
     5941 cctccatccc aacctggctc cctcccaccc aaccaacttt ccccccaacc cggaaacaga
     6001 caagcaaccc aaactgaacc ccctcaaaag ccaaaaaatg ggagacaatt tcacatggac
     6061 tttggaaaat atttttttcc tttgcattca tctctcaaac ttagttttta tctttgacca
     6121 accgaacatg accaaaaacc aaaagtgcat tcaaccttac caaaaaaaaa aaaaaaactc
     6181 tccagcgctg gatcccctgg tgctgatggt cctgctggtg ctcctggtac tccccctaga
     6241 aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc
     6301 ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga
     6361 ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca
     6421 ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc
     6481 tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg
     6541 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     6601 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     6661 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     6721 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
     6781 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     6841 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
     6901 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta
     6961 attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga
     7021 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
     7081 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc
     7141 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     7201 tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag
     7261 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt
     7321 atatccattt tcggatctga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa
     7381 gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg
     7441 gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc
     7501 ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca
     7561 gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc
     7621 actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca
     7681 tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat
     7741 acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca
     7801 cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg
     7861 ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc
     7921 gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct
     7981 ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct
     8041 acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac
     8101 ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc
     8161 tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag
     8221 atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg
     8281 ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact
     8341 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     8401 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     8461 atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc
     8521 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     8581 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     8641 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     8701 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     8761 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     8821 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     8881 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     8941 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     9001 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     9061 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     9121 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     9181 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     9241 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     9301 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     9361 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     9421 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     9481 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     9541 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     9601 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     9661 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     9721 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     9781 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     9841 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     9901 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     9961 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
    10021 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
    10081 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
    10141 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
    10201 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
    10261 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
    10321 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
    10381 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
    10441 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
    10501 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
    10561 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
    10621 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtc
//
