LOCUS       sequence-296795-         990 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  express CARPID BASU-dCasRx fusion protein in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 990)
  AUTHORS   Yi W, Li J, Zhu X, Wang X, Fan L, Sun W, Liao L, Zhang J, Li X, Ye 
            J, Chen F, Taipale J, Chan KM, Zhang L, Yan J
  TITLE     CRISPR-assisted detection of RNA-protein interactions in living 
            cells.
  JOURNAL   Nat Methods. 2020 Jun 22. pii: 10.1038/s41592-020-0866-0. doi: 
            10.1038/s41592-020-0866-0.
  PUBMED    32572232
REFERENCE   2  (bases 1 to 990)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2020 Jun 22. pii: 10.1038/s41592-020-0866-0. doi: 
            10.1038/s41592-020-0866-0."
COMMENT     Sequence Label: CARPID BASU-dCasRx
FEATURES             Location/Qualifiers
     source          1..990
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             822..842
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 cgtgacgtac ggccaccatg agcggcaagt tgtctgaatc agagattagg tttggactga
       61 aaacggaggt gatgggccaa catcttatct atcacgatgt tttgtccagc acacaaaaga
      121 cagcccatga attggcaaat aacaatgccc ccgagggtac cctcgtggtt gcagataaac
      181 agacagccgg taggggcggg atgagccgag tgtggcattc tcaagaagga aacggagtat
      241 ggatgagttt gatattgcgc ccggacattc cgctccaaaa gacgccccaa cttaccttgc
      301 tggcagctgt agcggtggtt cagggaatag aagaggccgc gggcatacag actgatataa
      361 agtggccgaa cgacatactc attaacggga aaaaaacagt agggatactt accgaaatgc
      421 aagctgagga ggatcgagtg cgctccgtga ttattggtat tggcattaat gtaaatcagc
      481 aacccaatga tttccccgat gagctcaagg acattgcgac gagtctgtcc caggcagctg
      541 gggaaaagat cgacagggca ggtgtaatcc agcatatctt gctttgtttc gaaaagcgat
      601 accgagacta catgacccac ggtttcacgc ccatcaagct cttgtgggag tcatacgccc
      661 tcggtattgg cacgaacatg agggcgagaa ctctcaacgg gacattttac ggtaaggcgt
      721 tgggtattga cgacgaaggg gtacttttgt tggaaactaa tgaaggcatt aagaagatat
      781 acagcgcgga catcagcctt aggcgtacgg ccaccatgag ccccaagaag aagagaaagg
      841 tggaggccag catcgaaaaa aaaaagtcct tcgccaaggg catgggcgtg aagtccacac
      901 tcgtgtccgg ctccaaagtg tacatgacaa ccttcgccga aggcagcgac gccaggctgg
      961 aaaagatcgt ggagggcgac agcatcagga
//
