LOCUS       sequence-296796-         994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  express CARPID dCasRx-BASU fusion protein in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Yi W, Li J, Zhu X, Wang X, Fan L, Sun W, Liao L, Zhang J, Li X, Ye 
            J, Chen F, Taipale J, Chan KM, Zhang L, Yan J
  TITLE     CRISPR-assisted detection of RNA-protein interactions in living 
            cells.
  JOURNAL   Nat Methods. 2020 Jun 22. pii: 10.1038/s41592-020-0866-0. doi: 
            10.1038/s41592-020-0866-0.
  PUBMED    32572232
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2020 Jun 22. pii: 10.1038/s41592-020-0866-0. doi: 
            10.1038/s41592-020-0866-0."
COMMENT     Sequence Label: CARPID dCasRx-BASU
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             26..46
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gtcgtgacgt acggccacca tgagccccaa gaagaagaga aaggtggagg ccagcatcga
       61 aaaaaaaaag tccttcgcca agggcatggg cgtgaagtcc acactcgtgt ccggctccaa
      121 agtgtacatg acaaccttcg ccgaaggcag cgacgccagg ctggaaaaga tcgtggaggg
      181 cgacagcatc aggagcgtga atgagggcga ggccttcagc gctgaaatgg ccgataaaaa
      241 cgccggctat aagatcggca acgccaaatt cagccatcct aagggctacg ccgtggtggc
      301 taacaaccct ctgtatacag gacccgtcca gcaggatatg ctcggcctga aggaaactct
      361 ggaaaagagg tacttcggcg agagcgctga tggcaatgac aatatttgta tccaggtgat
      421 ccataacatc ctggacattg aaaaaatcct cgccgaatac attaccaacg ccgcctacgc
      481 cgtcaacaat atctccggcc tggataagga cattattgga ttcggcaagt tctccacagt
      541 gtatacctac gacgaattca aagaccccga gcaccatagg gccgctttca acaataacga
      601 taagctcatc aacgccatca aggcccagta tgacgagttc gacaacttcc tcgataaccc
      661 cagactcggc tatttcggcc aggccttttt cagcaaggag ggcagaaatt acatcatcaa
      721 ttacggcaac gaatgctatg acattctggc cctcctgagc ggactggcgc actgggtggt
      781 cgctaacaac gaagaagagt ccaggatctc caggacctgg ctctacaacc tcgataagaa
      841 cctcgacaac gaatacatct ccaccctcaa ctacctctac gacaggatca ccaatgagct
      901 gaccaactcc ttctccaaga actccgccgc caacgtgaac tatattgccg aaactctggg
      961 aatcaaccct gccgaattcg ccgaacaata tttc
//
