LOCUS       sequence-296828-        5156 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bait vector AT2G01210 X007_pECIA2 should be used with prey vector 
            AT2G01210 X007_pECIA14..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5156)
  AUTHORS   Smakowska-Luzan E, Mott GA, Parys K, Stegmann M, Howton TC, 
            Layeghifard M, Neuhold J, Lehner A, Kong J, Grunwald K, Weinberger 
            N, Satbhai SB, Mayer D, Busch W, Madalinski M, Stolt-Bergner P, 
            Provart NJ, Mukhtar MS, Zipfel C, Desveaux D, Guttman DS, Belkhadir 
            Y
  TITLE     An extracellular network of Arabidopsis leucine-rich repeat receptor
            kinases.
  JOURNAL   Nature. 2018 Jan 18;553(7688):342-346. doi: 10.1038/nature25184. 
            Epub 2018 Jan 10.
  PUBMED    29320478
REFERENCE   2  (bases 1 to 5156)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature25184"; journalName: "Nature"; date: "2018-01-18- 
            18"; volume: "553"; issue: "7688"; pages: "342-346"
COMMENT     Sequence Label: AT2G01210 X007_pECIA2
FEATURES             Location/Qualifiers
     source          1..5156
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        23..449
                     /label=MT promoter
                     /note="Drosophila metallothionein promoter"
     sig_peptide     467..520
                     /codon_start=1
                     /product="Drosophila BiP signal sequence"
                     /label=BiP signal sequence
                     /translation="MKLCILLAVVAFVGLSLG"
     CDS             1409..1432
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             2138..2179
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             2189..2206
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(2229..2246)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2279..2413
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2303..2322)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2357..2376
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2557..2573)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2557..2573)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2570..2592)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2581..2597
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2605..2611,2612..2629,2630..2635))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2650..2671
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2788..2805)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2959..3547)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3039..3058)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3718..4509,4510..4578))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4341..4360
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4579..4683)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4751..4769
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(4807..4829)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4929..4948
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     join(5142..5156,1..8)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 gtaaaacgac ggccagtgaa ttaattcgtt gcaggacagg atgtggtgcc cgatgtgact
       61 agctctttgc tgcaggccgt cctatcctct ggttccgata agagacccag aactccggcc
      121 ccccaccgcc caccgccacc cccatacata tgtggtacgc aagtaagagt gcctgcgcat
      181 gccccatgtg ccccaccaag agctttgcat cccatacaag tccccaaagt ggagaaccga
      241 accaattctt cgcgggcaga acaaaagctt ctgcacacgt ctccactcga atttggagcc
      301 ggccggcgtg tgcaaaagag gtgaatcgaa cgaaagaccc gtgtgtaaag ccgcgtttcc
      361 aaaatgtata aaaccgagag catctggcca atgtgcatca gttgtggtca gcagcaaaat
      421 caagtgaatc atctcagtgc aactaaaggg gggatccgat ctcaatatga agttatgcat
      481 attactggcc gtcgtggcct ttgttggcct ctcgctcggg aacgacgaag gctttgcgct
      541 cttaacgttt aaacaaagcg ttcacgatga tcctacaggt tctcttaaca attggaactc
      601 atcagacgag aacgcctgtt cgtggaacgg tgtaacttgc aaagaactca gggtggtgtc
      661 tctaagcatt ccaaggaaga atctttacgg ttctctccct tcgtctctag ggtttctctc
      721 cagtctccgt cacttgaatc tccggagcaa taggttttat gggtcgttgc ctattcagct
      781 tttccatctt cagggactcc aaagtctagt cctttacggc aattcctttg atgggtcatt
      841 gtcggaagag atcggtaagc tgaagcttct tcagacctta gatttgtcac agaatctctt
      901 caacgggtcg ttaccacttt cgattctgca atgcaacaga cttaaaacac tcgatgtcag
      961 cagaaacaac ttgtctggtc ctttgcctga tgggtttggc tcagcgttcg tctctctgga
     1021 gaaactcgac ctcgccttca accaattcaa cggctccatc cccagcgaca ttggaaacct
     1081 ctccaacctt caaggaactg ctgatttctc tcataatcac ttcaccggct caatcccacc
     1141 tgcccttgga gatcttcctg aaaaggttta cattgatctc actttcaata atctctccgg
     1201 tcccattcct caaaccggtg ctcttatgaa cagaggacca acagcgttta tcggcaacac
     1261 tggtctatgc gggcctccat tgaaggacct ttgccaagga tatcaacttg gcctcaatgc
     1321 ctcttaccct tttatcccaa gcaacaaccc acctgaggat tctgatagta ctaactccga
     1381 aaccaaacag aaatcaagtg gtttgagcct ggaggtgctg ttccagggac cagggagcga
     1441 caaaactcac acatgcccac cgtgcccagc acctgaactc ctggggggac cgtcagtctt
     1501 cctcttcccc ccaaaaccca aggacaccct catgatctcc cggacccctg aggtcacatg
     1561 cgtggtggtg gacgtgagcc acgaagaccc tgaggtcaag ttcaactggt acgtggacgg
     1621 cgtggaggtg cataatgcca agacaaagcc gcgggaggag cagtacaaca gcacgtaccg
     1681 tgtggtcagc gtcctcaccg tcctgcacca ggactggctg aatggcaagg agtacaagtg
     1741 caaggtctcc aacaaagccc tcccagcccc catcgagaaa accatctcca aagccaaagg
     1801 gcagccccga gaaccacagg tgtacaccct gcccccatcc cgggaggaga tgaccaagaa
     1861 ccaggtcagc ctgacctgcc tggtcaaagg cttctatccc agcgacatcg ccgtggagtg
     1921 ggagagcaat gggcagccgg agaacaacta caagaccacg cctcccgtgc tggactccga
     1981 cggctccttc ttcctctaca gcaagctcac cgtggacaag agcaggtggc agcaggggaa
     2041 cgtcttctca tgctccgtga tgcatgaggg tctgcacaac cactacacgc agaagagcct
     2101 ctccctgtct ccgggtaaat ctagagggcc cttcgaaggt aagcctatcc ctaaccctct
     2161 cctcggtctc gattctacgc gtaccggtca tcatcaccat caccattgag tttaaacccg
     2221 ctgatcagcc tcgactgtgc cttctaaggc ctgagctcgc tgatcagcct cgatcgagga
     2281 tccagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa
     2341 aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg
     2401 caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt
     2461 gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtatggctg attatgatca
     2521 gtcgacctgc aggcatgcaa gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     2581 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     2641 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     2701 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     2761 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     2821 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     2881 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     2941 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     3001 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     3061 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     3121 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     3181 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     3241 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     3301 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     3361 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     3421 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     3481 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     3541 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     3601 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     3661 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     3721 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     3781 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     3841 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     3901 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     3961 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     4021 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     4081 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     4141 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     4201 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     4261 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     4321 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     4381 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     4441 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     4501 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     4561 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     4621 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     4681 gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt
     4741 aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg
     4801 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc
     4861 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct
     4921 taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc
     4981 gcacagatgc gtaaggagaa aataccgcat caggcgccat tcgccattca ggctgcgcaa
     5041 ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg
     5101 atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgtt
//
