LOCUS       sequence-296874-       11965 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  RSF1010-Cas9-pcaHG gRNA. Vector with constitutive Cas9 with gRNA 
            targeting pcaHG locus..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11965)
  AUTHORS   Biggs BW, Bedore SR, Arvay E, Huang S, Subramanian H, McIntyre EA, 
            Duscent-Maitland CV, Neidle EL, Tyo KEJ
  TITLE     Development of a genetic toolset for the highly engineerable and 
            metabolically versatile Acinetobacter baylyi ADP1.
  JOURNAL   Nucleic Acids Res. 2020 Apr 4. pii: 5815822. doi: 
            10.1093/nar/gkaa167.
  PUBMED    32246719
REFERENCE   2  (bases 1 to 11965)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Apr 4. pii: 5815822. doi: 10.1093/nar/gkaa167."
COMMENT     Sequence Label: pBWB419
FEATURES             Location/Qualifiers
     source          1..11965
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      381..775
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     primer_bind     1318..1336
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        join(1360..1374,1375..1380,1381..1397,1398..1404)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    1380..1396
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     misc_RNA        1431..1506
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     RBS             1758..1769
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             1777..5883
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRHKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     misc_feature    5904..6035
                     /label=crRNA leader
                     /note="crRNA leader sequence for the Streptococcus pyogenes
                     CRISPR/Cas system"
     repeat_region   6036..6071
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
     terminator      6087..6135
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     CDS             complement(6348..7139)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     CDS             complement(7519..8370)
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     CDS             complement(8357..9196)
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             complement(9707..10678)
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     oriT            join(11917..11965,1..39)
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
ORIGIN
        1 ggtcgggatt gccgccgctg tgcctccatg atagcctacg agacagcaca ttaacaatgg
       61 ggtgtcaaga tggttaaggg gagcaacaag gcggcggatc ggctggccaa gctcgaagaa
      121 caacgagcgc gaatcaatgc cgaaattcag cgggtgcggg caagggaaca gcagcaagag
      181 cgcaagaacg aaacaaggcg caaggtgctg gtgggggcca tgattttggc caaggtgaac
      241 agcagcgagt ggccggagga tcggctcatg gcggcaatgg atgcgtacct tgaacgcgac
      301 cacgaccgcg ccttgttcgg tctgccgcca cgccagaagg atgagccggg ctgaatgatc
      361 gaccgagaca ggccctgcgg ggctgcacac gcgcccccac ccttcgggta gggggaaagg
      421 ccgctaaagc ggctaaaagc gctccagcgt atttctgcgg ggtttggtgt ggggtttagc
      481 gggctttgcc cgcctttccc cctgccgcgc agcggtgggg cggtgtgtag cctagcgcag
      541 cgaatagacc agctatccgg cctctggccg ggcatattgg gcaagggcag cagcgcccca
      601 caagggcgct gataaccgcg cctagtggat tattcttaga taatcatgga tggatttttc
      661 caacaccccg ccagcccccg cccctgctgg gtttgcaggt ttgggggcgt gacagttatt
      721 gcaggggttc gtgacagtta ttgcaggggg gcgtgacagt tattgcaggg gttcgtgaca
      781 gttagtacgg gagtgacggg cactggctgg caatgtctag caacggcagg catttcggct
      841 gagggtaaaa gaactttccg ctaagcgata gactgtatgt aaacacagta ttgcaaggac
      901 gcggaacatg cctcatgtgg cggccaggac ggccagccgg gatcgggata ctggtcgtta
      961 ccagagccac cgacccgagc aaacccttct ctatcagatc gttgacgagt attacccggc
     1021 attcgctgcg cttatggcag agcagggaaa ggaattgccg ggctatgtgc aacgggaatt
     1081 tgaagaattt ctccaatgcg ggcggctgga gcatggcttt ctacgggttc gctgcgagtc
     1141 ttgccacgcc gagcacctgg tcgctttcag aaatcaatct aaagtatata tgagtaaact
     1201 tggtctgaca ggcccctgaa ttcgcatcta gactgaactg gccgataatt gcagacgaac
     1261 gcgcccttcc caacagttgc ctaagaaacc attattatca tgacattaac ctataaaaat
     1321 aggcgtatca cgaggccctt tcgtcttcac ctcgagaaat cataaaaaat ttatttgctt
     1381 tgtgagcgga taacaattat aatagattca gatccgaact ttttgggttg gttttagagc
     1441 tagaaatagc aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt
     1501 cggtgctttt tttgaattct ctagagtcga cctgcagaag cttagatcta ttaccctgtt
     1561 atccctactc gagttcatgt gcagctccat cagcatccgt aatcgttaat ccgcaaataa
     1621 cgtaaaaacc cgcttcggcg ggttttttta tggggggagt ttagggaaag agcatttgtc
     1681 agctgccaaa ccagatgtca acacagctac aacgcccagc ctccaattca aatcataaaa
     1741 aatttatttg ctttattaaa gaggagaaat taattaatgg ataagaaata ctcaataggc
     1801 ttagatatcg gcacaaatag cgtcggatgg gcggtgatca ctgatgaata taaggttccg
     1861 tctaaaaagt tcaaggttct gggaaataca gaccgccaca gtatcaaaaa aaatcttata
     1921 ggggctcttt tatttgacag tggagagaca gcggaagcga ctcgtcataa acggacagct
     1981 cgtagaaggt atacacgtcg gaagaatcgt atttgttatc tacaggagat tttttcaaat
     2041 gagatggcga aagtagatga tagtttcttt catcgacttg aagagtcttt tttggtggaa
     2101 gaagacaaga agcatgaacg tcatcctatt tttggaaata tagtagatga agttgcttat
     2161 catgagaaat atccaactat ctatcatctg cgaaaaaaat tggtagattc tactgataaa
     2221 gcggatttgc gcttaatcta tttggcctta gcgcatatga ttaagtttcg tggtcatttt
     2281 ttgattgagg gagatttaaa tcctgataat agtgatgtgg acaaactatt tatccagttg
     2341 gtacaaacct acaatcaatt atttgaagaa aaccctatta acgcaagtgg agtagatgct
     2401 aaagcgattc tttctgcacg attgagtaaa tcaagacgat tagaaaatct cattgctcag
     2461 ctccccggtg agaagaaaaa tggcttattt gggaatctca ttgctttgtc attgggtttg
     2521 acccctaatt ttaaatcaaa ttttgatttg gcagaagatg ctaaattaca gctttcaaaa
     2581 gatacttacg atgatgattt agataattta ttggcgcaaa ttggagatca atatgctgat
     2641 ttgtttttgg cagctaagaa tttatcagat gctattttac tttcagatat cctaagagta
     2701 aatactgaaa taactaaggc tcccctatca gcttcaatga ttaaacgcta cgatgaacat
     2761 catcaagact tgactctttt aaaagcttta gttcgacaac aacttccaga aaagtataaa
     2821 gaaatctttt ttgatcaatc aaaaaacgga tatgcaggtt atattgatgg gggagctagc
     2881 caagaagaat tttataaatt tatcaaacca attttagaaa aaatggatgg tactgaggaa
     2941 ttattggtga aactaaatcg tgaagatttg ctgcgcaagc aacggacctt tgacaacggc
     3001 tctattcccc atcaaattca cttgggtgag ctgcatgcta ttttgagaag acaagaagac
     3061 ttttatccat ttttaaaaga caatcgtgag aagattgaaa aaatcttgac ttttcgaatt
     3121 ccttattatg ttggtccatt ggcgcgtggc aatagtcgtt ttgcatggat gactcggaag
     3181 tctgaagaaa caattacccc atggaatttt gaagaagttg tcgataaagg tgcttcagct
     3241 caatcattta ttgaacgcat gacaaacttt gataaaaatc ttccaaatga aaaagtacta
     3301 ccaaaacata gtttgcttta tgagtatttt acggtttata acgaattgac aaaggtcaaa
     3361 tatgttactg aaggaatgcg aaaaccagca tttctttcag gtgaacagaa gaaagccatt
     3421 gttgatttac tcttcaaaac aaatcgaaaa gtaaccgtta agcaattaaa agaagattat
     3481 ttcaaaaaaa tagaatgttt tgatagtgtt gaaatttcag gagttgaaga tagatttaat
     3541 gcttcattag gtacctacca tgatttgcta aaaattatta aagataaaga ttttttggat
     3601 aatgaagaaa atgaagatat cttagaggat attgttttaa cattgacctt atttgaagat
     3661 agggagatga ttgaggaaag acttaaaaca tatgctcacc tctttgatga taaggtgatg
     3721 aaacagctta aacgtcgccg ttatactggt tggggacgtt tgtctcgaaa attgattaat
     3781 ggtattaggg ataagcaatc tggcaaaaca atattagatt ttttgaaatc agatggtttt
     3841 gccaatcgca attttatgca gctgatccat gatgatagtt tgacatttaa agaagacatt
     3901 caaaaagcac aagtgtctgg acaaggcgat agtttacatg aacatattgc aaatttagct
     3961 ggtagccctg ctattaaaaa aggtatttta cagactgtaa aagttgttga tgaattggtc
     4021 aaagtaatgg ggcggcataa gccagaaaat atcgttattg aaatggcacg tgaaaatcag
     4081 acaactcaaa agggccagaa aaattcgcga gagcgtatga aacgaatcga agaaggtatc
     4141 aaagaattag gaagtcagat tcttaaagag catcctgttg aaaatactca attgcaaaat
     4201 gaaaagctct atctctatta tctccaaaat ggaagagaca tgtatgtgga ccaagaatta
     4261 gatattaatc gtttaagtga ttatgatgtc gatcacattg ttccacaaag tttccttaaa
     4321 gacgattcaa tagacaataa ggtcttaacg cgttctgata aaaatcgtgg taaatcggat
     4381 aacgttccaa gtgaagaagt agtcaaaaag atgaaaaact attggagaca acttctaaac
     4441 gccaagttaa tcactcaacg taagtttgat aatttaacga aagctgaacg tggaggtttg
     4501 agtgaacttg ataaagctgg ttttatcaaa cgccaattgg ttgaaactcg ccaaatcact
     4561 aagcatgtgg cacaaatttt ggatagtcgc atgaatacta aatacgatga aaatgataaa
     4621 cttattcgag aggttaaagt gattacctta aaatctaaat tagtttctga cttccgaaaa
     4681 gatttccaat tctataaagt acgtgagatt aacaattacc atcatgccca tgatgcgtat
     4741 ctaaatgccg tcgttggaac tgctttgatt aagaaatatc caaaacttga atcggagttt
     4801 gtctatggtg attataaagt ttatgatgtt cgtaaaatga ttgctaagtc tgagcaagaa
     4861 ataggcaaag caaccgcaaa atatttcttt tactctaata tcatgaactt cttcaaaaca
     4921 gaaattacac ttgcaaatgg agagattcgc aaacgccctc taatcgaaac taatggggaa
     4981 actggagaaa ttgtctggga taaagggcga gattttgcca cagtgcgcaa agtattgtcc
     5041 atgccccaag tcaatattgt caagaaaaca gaagtacaga caggcggatt ctccaaggag
     5101 tcaattttac caaaaagaaa ttcggacaag cttattgctc gtaaaaaaga ctgggatcca
     5161 aaaaaatatg gtggttttga tagtccaacg gtagcttatt cagtcctagt ggttgctaag
     5221 gtggaaaaag ggaaatcgaa gaagttaaaa tccgttaaag agttactagg gatcacaatt
     5281 atggaaagaa gttcctttga aaaaaatccg attgactttt tagaagctaa aggatataag
     5341 gaagttaaaa aagacttaat cattaaacta cctaaatata gtctttttga gttagaaaac
     5401 ggtcgtaaac ggatgctggc tagtgccgga gaattacaaa aaggaaatga gctggctctg
     5461 ccaagcaaat atgtgaattt tttatattta gctagtcatt atgaaaagtt gaagggtagt
     5521 ccagaagata acgaacaaaa acaattgttt gtggagcagc ataagcatta tttagatgag
     5581 attattgagc aaatcagtga attttctaag cgtgttattt tagcagatgc caatttagat
     5641 aaagttctta gtgcatataa caaacataga gacaaaccaa tacgtgaaca agcagaaaat
     5701 attattcatt tatttacgtt gacgaatctt ggagctcccg ctgcttttaa atattttgat
     5761 acaacaattg atcgtaaacg atatacgtct acaaaagaag ttttagatgc cactcttatc
     5821 catcaatcca tcactggtct ttatgaaaca cgcattgatt tgagtcagct aggaggtgac
     5881 tgaagtatat tttagatgaa gattatttct taataactaa aaatatggta taatactctt
     5941 aataaatgca gtaatacagg ggcttttcaa gactgaagtc tagctgagac aaatagtgcg
     6001 attacgaaat tttttagaca aaaatagtct acgaggtttt agagctatgc tgttttgaat
     6061 ggtcccaaaa caaagcaagc tgataaaccg atacaattaa aggctccttt tggagccttt
     6121 ttttttggag attttcaaca tgaagaaatt attatttgat gatcagatag cggcggggaa
     6181 ctgccagaca tcaaataaaa caaaaggctc agtcggaaga ctgggccttt tgttttatct
     6241 gttgtttgtc ggtgaacact ctcccggctg agcagttaca gagatgttac gaacccccag
     6301 gacatccgag aatgcgaggc gatggagggt acaacccgag cggccgctta tttgccgact
     6361 accttggtga tctcgccttt cacgtagtgg acaaattctt ccaactgatc tgcgcgcgag
     6421 gccaagcgat cttcttcttg tccaagataa gcctgtctag cttcaagtat gacgggctga
     6481 tactgggccg gcaggcgctc cattgcccag tcggcagcga catccttcgg cgcgattttg
     6541 ccggttactg cgctgtacca aatgcgggac aacgtaagca ctacatttcg ctcatcgcca
     6601 gcccagtcgg gcggcgagtt ccatagcgtt aaggtttcat ttagcgcctc aaatagatcc
     6661 tgttcaggaa ccggatcaaa gagttcctcc gccgctggac ctaccaaggc aacgctatgt
     6721 tctcttgctt ttgtcagcaa gatagccaga tcaatgtcga tcgtggctgg ctcgaagata
     6781 cccgcaagaa tgtcattgcg ctgccattct ccaaattgca gttcgcgctt agctggataa
     6841 cgccacggaa tgatgtcgtc gtgcacaaca atggtgactt ctacagcgcg gagaatctcg
     6901 ctctctccag gggaagccga agtttccaaa aggtcgttga tcaaagctcg ccgcgttgtt
     6961 tcatcaagcc ttacggtcac cgtaaccagc aaatcaatat cactgtgtgg cttcaggccg
     7021 ccatccactg cggagccgta caaatgtacg gccagcaacg tcggttcgag atggcgctcg
     7081 atgacgccaa ctacctctga tagttgagtc gatacttcgg cgatcaccgc ttccctcata
     7141 atgtttaact ttgttttagg gcgactgccc tgctgcgtaa catcgttgct gctccataac
     7201 atcaaacatc gacccacggc gtaacgcgct tgctgcttgg atgcccgagg catagactgt
     7261 accccaaaaa aacagtcata acaagccatg aaaaccgcca ctgcgccgtt accaccgctg
     7321 cgttcggtca aggttctgga ccagttgcgt gagcgcatac gctacttgca ttacagctta
     7381 cgaaccgaac aggcttatgt ccactgggtt cgtgccttca tccgtttcca cggtgtgcgt
     7441 cacccggcaa ccttgggtag cagcgaagtc gaggcatttc tgtcctggct ggtcatgacc
     7501 aaaatccctt aacgtgagtc agcctgccgc cttgggccgg gtgatgtcgt acttgcccgc
     7561 cgcgaactcg gttaccgtcc agcccagcgc gaccagctcc ggcaacgcct cgcgcacccg
     7621 ctggcggcgc ttgcgcatgg tcgaaccact ggcctctgac ggccagacat agccgcacaa
     7681 ggtatctatg gaagccttgc cggttttgcc ggggtcgatc cagccacaca gccgctggtg
     7741 cagcaggcgg gcggtttcgc tgtccagcgc ccgcacctcg tccatgctga tgcgcacatg
     7801 ctggccgcca cccatgacgg cctgcgcgat caaggggttc agggccacgt acaggcgccc
     7861 gtccgcctcg tcgctggcgt actccgacag cagccgaaac ccctgccgct tgcggccatt
     7921 ctgggcgatg atggatacct tccaaaggcg ctcgatgcag tcctgtatgt gcttgagcgc
     7981 cccaccacta tcgacctctg ccccgatttc ctttgccagc gcccgatagc tacctttgac
     8041 caccatggca tcagcggtga cggcctccca cttgggttcc aggaacagcc ggagctgccg
     8101 tccgccttcg gtcttgggtt ccgggccaag cactaggcca ttaggcccag ccatggccac
     8161 cagcccttgc aggatgcgca gatcatcagc gcccagcggc tccgggccgc tgaactcgat
     8221 ccgcttgccg tcgccgtagt catacgtcac gtccagcttg ctgcgcttgc gctcgccccg
     8281 cttgagggca cggaacaggc cgggggccag acagtgcgcc gggtcgtgcc ggacgtggct
     8341 gaggctgtgc ttgttcttag gcttcaccac ggggcacccc cttgctcttg cgctgcctct
     8401 ccagcacggc gggcttgagc accccgccgt catgccgcct gaaccaccga tcagcgaacg
     8461 gtgcgccata gttggccttg ctcacaccga agcggacgaa gaaccggcgc tggtcgtcgt
     8521 ccacacccca ttcctcggcc tcggcgctgg tcatgctcga caggtaggac tgccagcgga
     8581 tgttatcgac cagtaccgag ctgccccggc tggcctgctg ctggtcgcct gcgcccatca
     8641 tggccgcgcc cttgctggca tggtgcagga acacgataga gcacccggta tcggcggcga
     8701 tggcctccat gcgaccgatg acctgggcca tggggccgct ggcgttttct tcctcgatgt
     8761 ggaaccggcg cagcgtgtcc agcaccatca ggcggcggcc ctcggcggcg cgcttgaggc
     8821 cgtcgaacca ctccggggcc atgatgttgg gcaggctgcc gatcagcggc tggatcagca
     8881 ggccgtcagc cacggcttgc cgttcctcgg cgctgaggtg cgccccaagg gcgtgcaggc
     8941 ggtgatgaat ggcggtgggc gggtcttcgg cgggcaggta gatcaccggg ccggtgggca
     9001 gttcgcccac ctccagcaga tccggcccgc ctgcaatctg tgcggccagt tgcagggcca
     9061 gcatggattt accggcacca ccgggcgaca ccagcgcccc gaccgtaccg gccaccatgt
     9121 tgggcaaaac gtagtccagc ggtggcggcg ctgctgcgaa cgcctccaga atattgatag
     9181 gcttatgggt agccattgat tgcctccttt gcaggcagtt ggtggttagg cgctggcggg
     9241 gtcactaccc ccgccctgcg ccgctctgag ttcttccagg cactcgcgca gcgcctcgta
     9301 ttcgtcgtcg gtcagccaga acttgcgctg acgcatccct ttggccttca tgcgctcggc
     9361 atatcgcgct tggcgtacag cgtcagggct ggccagcagg tcgccggtct gcttgtcctt
     9421 ttggtctttc atatcagtca ccgagaaact tgccggggcc gaaaggcttg tcttcgcgga
     9481 acaaggacaa ggtgcagccg tcaaggttaa ggctggccat atcagcgact gaaaagcggc
     9541 cagcctcggc cttgtttgac gtataaccaa agccaccggg caaccaatag cccttgtcac
     9601 ttttgatcag gtagaccgac cctgaagcgc ttttttcgta ttccataaaa cccccttctg
     9661 tgcgtgagta ctcatagtat aacaggcgtg agtaccaacg caagcactac atgctgaaat
     9721 ctggcccgcc cctgtccatg cctcgctggc ggggtgccgg tgcccgtgcc agctcggccc
     9781 gcgcaagctg gacgctgggc agacccatga ccttgctgac ggtgcgctcg atgtaatccg
     9841 cttcgtggcc gggcttgcgc tctgccagcg ctgggctggc ctcggccatg gccttgccga
     9901 tttcctcggc actgcggccc cggctggcca gcttctgcgc ggcgataaag tcgcacttgc
     9961 tgaggtcatc accgaagcgc ttgaccagcc cggccatctc gctgcggtac tcgtccagcg
    10021 ccgtgcgccg gtggcggcta agctgccgct cgggcagttc gaggctggcc agcctgcggg
    10081 ccttctcctg ctgccgctgg gcctgctcga tctgctggcc agcctgctgc accagcgccg
    10141 ggccagcggt ggcggtcttg cccttggatt cacgcagcag cacccacggc tgataaccgg
    10201 cgcgggtggt gtgcttgtcc ttgcggttgg tgaagcccgc caagcggcca tagtggcggc
    10261 tgtcggcgct ggccgggtcg gcgtcgtact cgctggccag cgtccgggca atctgccccc
    10321 gaagttcacc gcctgcggcg tcggccacct tgacccatgc ctgatagttc ttcgggctgg
    10381 tttccactac cagggcaggc tcccggccct cggctttcat gtcatccagg tcaaactcgc
    10441 tgaggtcgtc caccagcacc agaccatgcc gctcctgctc ggcgggcctg atatacacgt
    10501 cattgccctg ggcattcatc cgcttgagcc atggcgtgtt ctggagcact tcggcggctg
    10561 accattcccg gttcatcatc tggccggtgg tggcgtccct gacgccgata tcgaagcgct
    10621 cacagcccat ggccttgagc tgtcggccta tggcctgcaa agtcctgtcg ttcttcatcg
    10681 ggccaccaag cgcagccaga tcgagccgtc ctcggttgtc agtggcgtca ggtcgagcaa
    10741 gagcaacgat gcgatcagca gcaccaccgt aggcatcatg gaagccagca tcacggttag
    10801 ccatagcttc cagtgccacc cccgcgacgc gctccgggcg ctctgcgcgg cgctgctcac
    10861 ctcggcggct acctcccgca actctttggc cagctccacc catgccgccc ctgtctggcg
    10921 ctgggctttc agccactccg ccgcctgcgc ctcgctggcc tgctgggtct ggctcatgac
    10981 ctgccgggct tcgtcggcca gtgtcgccat gctctgggcc agcggttcga tctgctccgc
    11041 taactcgttg atgcctctgg atttcttcac tctgtcgatt gcgttcatgg tctattgcct
    11101 cccggtattc ctgtaagtcg atgatctggg cgttggcggt gtcgatgttc agggccacgt
    11161 ctgcccggtc ggtgcggatg ccccggcctt ccatctccac cacgttcggc cccaggtgaa
    11221 caccgggcag gcgctcgatg ccctgcgcct caagtgttct gtggtcaatg cgggcgtcgt
    11281 ggccagcccg ctctaatgcc cggttggcat ggtcggccca tgcctcgcgg gtctgctcaa
    11341 gccatgcctt gggcttgagc gcttcggtct tctgtgcccc gcccttctcc ggggtcttgc
    11401 cgttgtaccg cttgaaccac tgagcggcgg gccgctcgat gccgtcattg atccgctcgg
    11461 agatcatcag gtggcagtgc gggttctcgc cgccaccggc atggatggcc agcgtatacg
    11521 gcaggcgctc ggcaccggtc aggtgctggg cgaactcgga cgccagcgcc ttctgctggt
    11581 cgagggtcag ctcgaccggc agggcaaatt cgacctcctt gaacagccgc ccattggcgc
    11641 gttcatacag gtcggcagca tcccagtagt cggcgggccg ctcgacgaac tccggcatgt
    11701 gcccggattc ggcgtgcaag acttcatcca tgtcgcgggc atacttgcct tcgcgctgga
    11761 tgtagtcggc cttggccctg gccgattggc cgcccgacct gctgccggtt ttcgccgtaa
    11821 ggtgataaat cgccatgctg cctcgctgtt gcttttgctt ttcggctcca tgcaatggcc
    11881 ctcggagagc gcaccgcccg aagggtggcc gttaggccag tttctcgaag agaaaccggt
    11941 aagtgcgccc tcccctacaa agtag
//
