LOCUS       sequence-296910-       12168 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway compatible expression plasmid for Drosophila and 
            establishing stable cell lines by Hygromycin selection. Actin5c 
            ubiquitous promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 12168)
  AUTHORS   Bosch JA, Birchak G, Perrimon N
  TITLE     Precise genome engineering in Drosophila using prime editing.
  JOURNAL   Proc Natl Acad Sci U S A. 2021 Jan 5;118(1). pii: 2021996118. doi: 
            10.1073/pnas.2021996118.
  PUBMED    33443210
REFERENCE   2  (bases 1 to 12168)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2021-01-5"; volume: "118(1). pii: 
            2021996118. doi"; pages: " 10.1073/pnas.2021996118"
COMMENT     Sequence Label: pAct-GW-HygroR
FEATURES             Location/Qualifiers
     source          1..12168
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(624..641)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(795..1383)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(875..894)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1554..2345,2346..2414))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2177..2196
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2415..2519)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2587..2605
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4001..4280
                     /label=copia promoter
                     /note="strong promoter from the Drosophila transposable 
                     element copia (Sinclair et al., 1986)"
     CDS             4326..5351
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             4326..5351
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     intron          5714..5779
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             5909..5929
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    6354..6488
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6391..6410)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6445..6464
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        6539..9048
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     protein_bind    9164..9288
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(9313..9318,9319..9336,9337..9343)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             9397..10056
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(9463..9482)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             10398..10703
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     10582..10601
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(10744..10868)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 cccgcgttgc aggccatgct gtccaggcag gtagatgacg accatcaggg acagcttcaa
       61 ggatcgctcg cggctcttac cagcctaact tcgatcattg gaccgctgat cgtcacggcg
      121 atttatgccg cctcggcgag cacatggaac gggttggcat ggattgtagg cgccgcccta
      181 taccttgtct gcctccccgc gttgcgtcgc ggtgcatgga gccgggccac ctcgacctga
      241 atggaagccg gcggcacctc gctaacggat tcaccactcc aagaattgga gccaatcaat
      301 tcttgcggag aactgtgaat gcgcaaacca acccttggca gaacatatcc atcgcgtccg
      361 ccatctccag cagccgcacg cggcgcatct cgggcagcgt tgggtcctgg ccacgggtgc
      421 gcatgatcgt gctcctgtcg ttgaggaccc ggctaggctg gcggggttgc cttactggtt
      481 agcagaatga atcaccgata cgcgagcgaa cgtgaagcga ctgctgctgc aaaacgtctg
      541 cgacctgagc aacaacatga atggtcttcg gtttccgtgt ttcgtaaagt ctggaaacgc
      601 ggaagtcagc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
      661 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
      721 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
      781 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
      841 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
      901 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
      961 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     1021 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     1081 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     1141 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     1201 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg
     1261 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     1321 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     1381 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     1441 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     1501 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     1561 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     1621 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     1681 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     1741 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     1801 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     1861 gccattgctg caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     1921 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     1981 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     2041 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     2101 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     2161 ccggcgtcaa cacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     2221 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     2281 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     2341 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     2401 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     2461 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     2521 acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc
     2581 tataaaaata ggcgtatcac gaggcccttt cgtcttcaag aattcacatt tgtacgaatt
     2641 ttttttttat caaaagttcg agtttttcac caatttcctc atcaaccgag caaggcaaac
     2701 ggctttgaat aatatggtgt tatatataca tatatcaaat cgctgctgac tgcgtgattg
     2761 atggccccaa gattacatat tatcgaatca ggattcagaa ggagatcaat gtcaaatgcg
     2821 gacaggaaca tgaaagacgc ctgttatgcg caattaaaaa tttgggttta attgctgtgg
     2881 aaactgttgt tggcggcatc ttaagttcct gtttaacaac atcaactact tatgtacgta
     2941 gaagcgttta agccatttgc atacagatga gaactggctt ttgtgctaat cagtcaagat
     3001 gactccgatg atgatgactc attacctgac cagttttcgc tgctttcttt tcaacaacta
     3061 cttgtatatg tattgtatcc aatagcaata cattgaattt ccatggtcta gtcacgtatt
     3121 atcatttaat tgacaccaag tcgtgttatt gttgagctat cgagttcagc tcaaacattt
     3181 cttattccca tgaataagcc ggcaaaaata tgcaatctat gaaagttaat ataagcaaac
     3241 cttactttga ctcaatacca atgcactttg tgtcgatagg ttcacgcaat tgaggcgatt
     3301 attccgataa cccaagcgat tgactgttcc cgtttcgatt ccaattgaaa tttggaaatg
     3361 tacaatagtt ttgctatatg ctgtcaagta cgctcttatc ttctctgggt tttcttcaga
     3421 gtttcgaaac gcttcttctt ttttttgttt tttttttttt ggaatctcgt attttggaag
     3481 gggctcccct ctggaatttg ttacactgtc gttatcattg cgaacaagcg gcccgaagct
     3541 atcagcgact ttaacattta caatgcactt ttttacgacc aattaaatgt acattttcct
     3601 ttcttcgccc gttgataagc gaacgcgatg tggcgcaggc aatgtgttgc tcttgcgaca
     3661 caaacgcaat caaaatggat tcaatttcgc tttttcccag tgaaacgaag aacgaaccga
     3721 ccatcatgat atgctcctct gcatgttgcg tattgaatca atgacaattt caattaagcc
     3781 gcccgttcgt catgcgtttt cgtgcgcttc gaaatgctga taacgctgct gtcctccaac
     3841 tgctttgcat gtggacacaa ttccatttat ttaattcttt tatttggatc ggttaaatta
     3901 aaaagcgcct tgttacgcat ttaacgttgt ttccggtgcg tggtggtttc atgcttctgg
     3961 gaacggcaaa tgggtttagg attgggaacc cctcatcatc tgttggaata tactattcaa
     4021 cctacaaaag taacgttaaa caacactact ttatatttga tatgaatggc cacacctttt
     4081 atgccataaa acatattgta agagaatacc actcttttta ttccttcttt ccttcttgta
     4141 cgttttttgc tgtaagtagg tcgtggtgct ggtgttgcag ttgaaataac ttaaaatata
     4201 aatcataaaa ctcaaacata aacttgacta tttatttatt tattaagaaa ggaaatataa
     4261 attataaatt acaacaggtt atggacctgc agccaagctt ggcgctcgtc cgggggcaat
     4321 gagatatgaa aaagcctgaa ctcaccgcga cgtctgtcga gaagtttctg atcgaaaagt
     4381 tcgacagcgt ctccgacctg atgcagctct cggagggcga agaatctcgt gctttcagct
     4441 tcgatgtagg agggcgtgga tatgtcctgc gggtaaatag ctgcgccgat ggtttctaca
     4501 aagatcgtta tgtttatcgg cactttgcat cggccgcgct cccgattccg gaagtgcttg
     4561 acattgggga attcagcgag agcctgacct attgcatctc ccgccgtgca cagggtgtca
     4621 cgttgcaaga cctgcctgaa accgaactgc ccgctgttct gcagccggtc gcggaggcca
     4681 tggatgcgat cgctgcggcc gatcttagcc agacgagcgg gttcggccca ttcggaccgc
     4741 aaggaatcgg tcaatacact acatggcgtg atttcatatg cgcgattgct gatccccatg
     4801 tgtatcactg gcaaactgtg atggacgaca ccgtcagtgc gtccgtcgcg caggctctcg
     4861 atgagctgat gctttgggcc gaggactgcc ccgaagtccg gcacctcgtg cacgcggatt
     4921 tcggctccaa caatgtcctg acggacaatg gccgcataac agcggtcatt gactggagcg
     4981 aggcgatgtt cggggattcc caatacgagg tcgccaacat cttcttctgg aggccgtggt
     5041 tggcttgtat ggagcagcag acgcgctact tcgagcggag gcatccggag cttgcaggat
     5101 cgccgcggct ccgggcgtat atgctccgca ttggtcttga ccaactctat cagagcttgg
     5161 ttgacggcaa tttcgatgat gcagcttggg cgcagggtcg atgcgacgca atcgtccgat
     5221 ccggagccgg gactgtcggg cgtacacaaa tcgcccgcag aagcgcggcc gtctggaccg
     5281 atggctgtgt agaagtactc gccgatagtg gaaaccgacg ccccagcact cgtccgaggg
     5341 caaaggaata gagtagatgc cgaccgaaca agagctgatt tcgagaacgc ctcagccagc
     5401 aactcgcgcg agcctagcaa ggcaaatgcg agagaacggc cttacgcttg gtggcacagt
     5461 tctcgtccac agttcgctaa gctcgctcgg ctgggtcgcg ggagggccgg tcgcagtgat
     5521 tcaggccctt ctggattgtg ttggtcccca gggcacgatt gtcatgccca cgcactcggg
     5581 tgatctgact gatcccgcag attggagatc gccgcccgtg cctgccgatt gggtgcagat
     5641 ctttgtgaag gaaccttact tctgtggtgt gacataattg gacaaactac ctacagagat
     5701 ttaaagctct aaggtaaata taaaattttt aagtgtataa tgtgttaaac tactgattct
     5761 aattgtttgt gtattttaga ttccaaccta tggaactgat gaatgggagc agtggtggaa
     5821 tgcctttaat gaggaaaacc tgttttgctc agaagaaatg ccatctagtg atgatgaggc
     5881 tactgctgac tctcaacatt ctactcctcc aaaaaagaag agaaaggtag aagaccccaa
     5941 ggactttcct tcagaattgc taagtttttt gagtcatgct gtgtttagta atagaactct
     6001 tgcttgcttt gctatttaca ccacaaagga aaaagctgca ctgctataca agaaaattat
     6061 ggaaaaatat tctgtaacct ttataagtag gcataacagt tataatcata acatactgtt
     6121 ttttcttact ccacacaggc atagagtgtc tgctattaat aactatgctc aaaaattgtg
     6181 tacctttagc tttttaattt gtaaaggggt taataaggaa tatttgatgt atagtgcctt
     6241 gactagagat cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc
     6301 tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt
     6361 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     6421 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     6481 tctggatcgc atgcaattct atattctaaa aacacaaatg atacttctaa aaaaaaatca
     6541 tgaatggcat caactctgaa tcaaatcttt gcagatgcac ctacttctca tttccactgt
     6601 cacatcattt ttccagatct cgctgcctgt tatgtggccc acaaaccaag acacgtttta
     6661 tggccattaa agctggctga tcgtcgccaa acaccaaata cataatgaat atgtacacat
     6721 tcgagaaaga agcgatcaaa gaagcgtctt cgggcggagt aggagaatgc ggaggagaag
     6781 gagaacgagc tgatctagta tctctccaca atccaatgcc aactgaccaa ctggccatat
     6841 tcggagcaat ttgaagccaa tttccatcgc ctggcgatcg ctccattctt ggctatatgt
     6901 ttttcaccgt tacccggggc cattttcaaa gactcgtcgg caagataaga ttgtgtcact
     6961 cgctgtctct cttcatttgt cgaagaatgc tgaggaattt cgcgatgacg tcggcgagta
     7021 ttttgaagaa tgagaataat ttgtatttat acgaaaatca gttagtggaa ttttctacaa
     7081 aaacatgtta tctatagata attttgttgc aaaatatgtt gactatgaca aagattgtat
     7141 gtatatacct ttaatgtatt ctcattttct tatgtattta taatggcaat gatgatactg
     7201 atgatatttt aagatgatgc cagaccaaaa ggcttgaatt tctgcgtctt ttgccgaacg
     7261 cagtgcatgt gcaattgttg ttttttggaa tattcaattt tcggactgtc cgctttgatt
     7321 tcagtttctt ggcttattca aaaagcaaag taaagccaaa aaagcgagat ggcaatacca
     7381 aatgcggcaa aacggtagtg gaaggaaagg ggtgcggggc agcggaagga agggtggggc
     7441 ggggcgtggc ggggtctgtg gctgggcgcg acgtcaccga cgttggagcc actcctttga
     7501 ccatgtgtgc gtgtgtgtat tattcgtgtc tcgccactcg ccggttgttt ttttcttttt
     7561 atgctgcgct ctctctagcg ccatctcgct tacgcatgct caacgcaccg catgttgccg
     7621 tttcctttta tgcgtcattt tggctcgaaa taggcaatta tttaaacaaa gattagtcaa
     7681 cgaaaacgct aaaataaata agtctacaat atggttactt attgccatgt gtgtgcagcc
     7741 aacgatagca acaaaagcaa caacacaggt ggctttccct ctttcacttt ttgtttgcaa
     7801 gccgcgtgcg agcaagacgg cacgaccggc aaacgcaatt acgctgacaa agagcagacg
     7861 aagttttggc gaaaaacatc aaggcgcctg atacgaatgc atttgcaata acaattgcga
     7921 tatttaatat tgtttatgaa gctgtttgac ttcaaaacac acaaaaaaaa aaataaaaca
     7981 aattatttga aagagaatta ggaatcggac gcttatcgtt agggtaacaa caagaaatgc
     8041 ttactgagtc acagcctctg gaaaactgcc gcaagccaga gagagagaga aaaagaggga
     8101 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tctgttgcgc
     8161 aaacgcatag actgcactga gaaaatcgat tacctatttt ttatgaatga atatttgcac
     8221 tattactatt caaaactatt aagatagcaa tcacattcaa tagccaaata ctataccacc
     8281 tgagcgatgc aacgaaatga tcaatttgag caaaaatgct gcatatttag gacggcatca
     8341 ttatagaaat gcttcttgct gtgtactttt ctctcgtctg gcagctgttt cgccgttatt
     8401 gttaaaaccg gcttaagtta ggtgtgtttt ctacgactag tgaatgccct actagaagat
     8461 gtgtgttgca caaaatgtcc ctggaataac caatttgaag tgcagatagc agtaaacgta
     8521 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     8581 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     8641 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     8701 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     8761 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     8821 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     8881 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     8941 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     9001 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatccaga
     9061 taccgagagc atctggccaa tgtgcatcag ttgtggtcag cagcaaaatc aagtgaatca
     9121 tctcagtgca actaaagggg ggatctcgag ggatccgata tcaacaagtt tgtacaaaaa
     9181 agctgaacga gaaacgtaaa atgatataaa tatcaatata ttaaattaga ttttgcataa
     9241 aaaacagact acataatact gtaaaacaca acatatccag tcactatggc ggccgcatta
     9301 ggcaccccag gctttacact ttatgcttcc ggctcgtata atgtgtggat tttgagttag
     9361 gatccgtcga gattttcagg agctaaggaa gctaaaatgg agaaaaaaat cactggatat
     9421 accaccgttg atatatccca atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt
     9481 gctcaatgta cctataacca gaccgttcag ctggatatta cggccttttt aaagaccgta
     9541 aagaaaaata agcacaagtt ttatccggcc tttattcaca ttcttgcccg cctgatgaat
     9601 gctcatccgg aattccgtat ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt
     9661 cacccttgtt acaccgtttt ccatgagcaa actgaaacgt tttcatcgct ctggagtgaa
     9721 taccacgacg atttccggca gtttctacac atatattcgc aagatgtggc gtgttacggt
     9781 gaaaacctgg cctatttccc taaagggttt attgagaata tgtttttcgt ctcagccaat
     9841 ccctgggtga gtttcaccag ttttgattta aacgtggcca atatggacaa cttcttcgcc
     9901 cccgttttca ccatgggcaa atattatacg caaggcgaca aggtgctgat gccgctggcg
     9961 attcaggttc atcatgccgt ttgtgatggc ttccatgtcg gcagaatgct taatgaatta
    10021 caacagtact gcgatgagtg gcagggcggg gcgtaaacgc gtggatccgg cttactaaaa
    10081 gccagataac agtatgcgta tttgcgcgct gatttttgcg gtataagaat atatactgat
    10141 atgtataccc gaagtatgtc aaaaagaggt atgctatgaa gcagcgtatt acagtgacag
    10201 ttgacagcga cagctatcag ttgctcaagg catatatgat gtcaatatct ccggtctggt
    10261 aagcacaacc atgcagaatg aagcccgtcg tctgcgtgcc gaacgctgga aagcggaaaa
    10321 tcaggaaggg atggctgagg tcgcccggtt tattgaaatg aacggctctt ttgctgacga
    10381 gaacaggggc tggtgaaatg cagtttaagg tttacaccta taaaagagag agccgttatc
    10441 gtctgtttgt ggatgtacag agtgatatta ttgacacgcc cgggcgacgg atggtgatcc
    10501 ccctggccag tgcacgtctg ctgtcagata aagtctcccg tgaactttac ccggtggtgc
    10561 atatcgggga tgaaagctgg cgcatgatga ccaccgatat ggccagtgtg ccggtctccg
    10621 ttatcgggga agaagtggct gatctcagcc accgcgaaaa tgacatcaaa aacgccatta
    10681 acctgatgtt ctggggaata taaatgtcag gctcccttat acacagccag tctgcaggtc
    10741 gaccatagtg actggatatg ttgtgtttta cagtattatg tagtctgttt tttatgcaaa
    10801 atctaattta atatattgat atttatatca ttttacgttt ctcgttcagc tttcttgtac
    10861 aaagtggttg atatcactag ttctagtcga ccatgaagat caagatcatt gccccgccag
    10921 agcgcaagta ctctgtctgg atcggtggct ccatcctggc ttcgctgtcc accttccagc
    10981 agatgtggat ctccaagcag gagtacgacg agtccggccc ctccattgtg caccgcaagt
    11041 gcttctaaga aggatcgctt gtctgggcaa gaggatcagg atcgggatgg tcttgattct
    11101 gctggaggag gaggaggaga agtcgaggaa gcagcagcga aagtgcaagt gcgagtggtg
    11161 gaagtttgga gtgcagcaca acaaaatcaa caacaacacc aactacaaga tgaaaagagc
    11221 ggaaccacct gcacaccatc atcactatca tcatcgtttt gggcgcatgt tgtgtggttc
    11281 cagcgtatta atataattaa tttattccac atgagatatg atatgatata ctatgtattt
    11341 tttgtttttt ttttatttgt aaacctttaa tataacaaga actacaaaaa atgaaaatga
    11401 gcgaaaatgc atattctgcc attccacaca cacaccaaca acacccaaca cacgcacacc
    11461 cacaagctta cacacacaca ttcgcggcat gacaaggaca tcaagataaa gaagaactta
    11521 aagaagatat ttcccaaagc gcaaaaagaa cacacacaca ttgcaaaaca caaacaacac
    11581 actagcgttt tgtacaattc gtcagcaacc ttatgtatta ttttttaatt atgatgtaat
    11641 tataaacaaa gtgaaacaaa aatatgaaaa caaaaaggaa aatcaaatct gtcttctctt
    11701 tctcccgctc tcctcgctct ctgctgctaa cctcgccctc tcctctctca tctttttgtc
    11761 tgtctctctt ccacattttt gccggccggc aaaataataa cccacacaca ctcacacttg
    11821 gctgcagttt cgcgtgcgat attcacacac attcaagcat acaagcatac atacatatgt
    11881 attttttttt tatttgtaca cttttctaat tgcatgcgta tcgattgata agtttacgcc
    11941 tgaaaatgtt aattaaaatg tgaaaatgca actgaaaaac tgatgaaatg aaacaacaac
    12001 aagcgaacaa tttgctacat gtgtattgtc taacaaccgt tactgcaacg gttgcttcgg
    12061 aaaagggtga agaggaagag ggcacgaggt cgactagagc ggccgccgac gcgaggctgg
    12121 atggccttcc ccattatgat tcttctcgct tccggcggca tcgggatg
//
