LOCUS       sequence-296918-        4292 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mobius Assembly cloning and expression vector for plants with 
            high-copy number origin of replication.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4292)
  AUTHORS   Andreou , Andreas I., Nirkko, Jessica , Ochoa-Villarreal, Marisol , 
            Nakayama, Naomi
  TITLE     Mobius Assembly for Plant Systems highlights promoter-terminator 
            interaction in gene regulation
  JOURNAL   bioRxiv, March 31, 2021
REFERENCE   2  (bases 1 to 4292)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv, 
            March 31, 2021"
COMMENT     Sequence Label: pMAP L1B
FEATURES             Location/Qualifiers
     source          1..4292
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      51..639
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     540..559
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    2161..2185
                     /label=LB T-DNA repeat
                     /note="left border repeat from octopine Ach5 T-DNA"
     misc_feature    2212..2236
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        2289..2323
                     /label=BBa_J23106
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.47"
     RBS             2332..2343
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      3032..3103
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3119..3146
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(3376..4191)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     4183..4202
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
ORIGIN
        1 gatcggattg gcggttatgc ggttcccgta gaaaagatca aaggatcttc ttgagatcct
       61 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
      121 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
      181 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
      241 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
      301 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
      361 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
      421 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
      481 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
      541 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
      601 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
      661 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
      721 gattctgtgg ataaccgtat taccgccttt gagtgagctg ccgatccgcc gccagttcag
      781 gccgtccggc cccggattct gaccataatt tcatcgaaaa aaggggcgca gcccttcttg
      841 ttctaatagt tctataagtt caggcgaaaa tcgtgcagca attacaaaag gttgcgcgtc
      901 tataagtggg gaatccagcc gcaaaagtgg ggaatccagc cgcaaaagtg gggaatccag
      961 ccgaaatcgc ggattgacga gtgggatttc ccgcccaata aatccaccat gggaaagaca
     1021 ctcgacgttg cccgcgaccg ggcctttgac cagaccgcga ccgtgctgcc cgccgaaatg
     1081 gcgcgggggg tctatatgcg caacgccccc agcctcgcgg ccctgaagct gatgcatctg
     1141 atgatcgcca cggcgggcgg gcgcatggcc gatgacgtgc gccacgaaat gcggctggcc
     1201 gacatccgca agatcgacgg tatggataac cacacccggg ccagcctgac cccgctcttt
     1261 gcggaactgg cggcggcggt gctgacccac gacgacccgg aaaagcgggt cgtgaccatc
     1321 ggtggcctgc tggacgaagc ccggatcgat taccggcacg aggtcagcgg cgatcttctg
     1381 gtgtcgtgga ccttccgcag catgttccgc cgcatggcgg cggaatcgaa ccattgggcg
     1441 attctcgacc ggcagaccgt gttccacctc ggcagcaagt attccgtgct gttgttccag
     1501 cacatcgcca gcttcaagga atacgaccac attaccggca agacctttac cgtgccggag
     1561 ttgcgggctg tgtttggtat ccccgagggc aaaatcaagc gtttcgcaga cctcaacaga
     1621 gacgtgctga cgcccgccat tgccgaaatc aaccagcttt cccgcctgac tctgaccgcc
     1681 acgccgaaca agatcgggcg caccgtggcc agcgtgacga ttgcttggga agaaaagccc
     1741 ctcgaaggca agcgctcgac caaggccgaa ctggaccgcc cgaaggtggg ccggaaggcc
     1801 cggcgcgacg gcaccgccga gacggtggca cgggccttcc cggcatcggg cgggatcgag
     1861 ttcgaccagc attggcgcga cctcaagcgg gcggcgggct gcaacatgga caacaccatg
     1921 atcgccgaca aattccgggc atggtgcgcc gggaagggcc tcgctctcga tgcccggaac
     1981 atcgaacagg cgttcagcag cttctgcacc aaggtgggcc gggtctgacc atcatcagtt
     2041 cggtggtctt ccgacgaaca ataaggccgc aaatcgcggc cttttttatt gataacaaaa
     2101 ccggctcagt tctgcgtaga aaccaacatg caagctccac cgggtgcaaa gcggcagcgg
     2161 cggcaggata tattcaattg taaatggctt catgtccggg aaatctactg gtggcaggat
     2221 atattgtggt gtaaacaatg gagaaaaaga gaattggaat tccacctgca tatcagagga
     2281 gagagacctt tacggctagc tcagtcctag gtatagtgct agctactaga gaaagaggag
     2341 aaatactaaa tgtcgcactc aaaacaagca ctggcagata cgatgaaaat gacgtggctg
     2401 atggaaggtt cggtcaacgg tcacgccttt acgattgaag gcgaaggtac cggcaaaccg
     2461 tatgaaggta aacagagtgg cacctttcgt gttacgaaag gcggtccgct gccgtttgcg
     2521 tttgatattg tcgctccgac cctgaaatac ggtttcaaat gcttcatgaa atacccggcg
     2581 gatattccgg actactttaa actggccttc ccggaaggcc tgacgtacga tcgtaaaatt
     2641 gcgtttgaag acggcggttg tgcgaccgcc acggtcgaaa tgagcctgaa gggtaacacc
     2701 ctggtgcata aaacgaactt tcagggcggt aatttcccga ttgatggtcc ggtgatgcaa
     2761 aaacgcaccc tgggctggga accgacctct gaaaaaatga cgccgtgcga tggtattatc
     2821 aaaggcgaca ccatcatgta cctgatggtt gaaggcggta aaacgctgaa atgtcgttat
     2881 gaaaacaatt accgcgccaa caaaccagtg ctgatgccgc cgagccactt tgtggatctg
     2941 cgtctgaccc gcacgaatct ggataaagaa ggtctggcgt tcaaactgga agaatatgct
     3001 gttgcccgtg tgctggaagt gtaataagct tcaaataaaa cgaaaggctc agtcgaaaga
     3061 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg
     3121 ctcaccttcg ggtgggcctt tctgcgggtc tcacgctgtc aatatgcagg tgctgcagga
     3181 attgttgatt ttgtgatgac tgatggcagg atatatgcgg ttgtaattca tttttattgt
     3241 ctaaatttct gtatttgttt gtttgttcgg ttgtaaattt ttttggaaga acaagaaaag
     3301 aaaaaacacc cgttagggtg tttttagtta gtgtggcgcg ccgacttgcg acatgcggtc
     3361 ctttgcaatc aactattaga aaaattcatc cagcatcaga tgaaattgca gtttgttcat
     3421 atccggatta tcaatgccat atttctgaaa cagacgtttt tgcaggctcg ggctaaattc
     3481 gcccaggcag ttccacagaa tggccagatc ctgataacga tccgcaatgc ccacacggcc
     3541 cacatcaatg cagccaatca gtttgccttc atcgaaaatc aggttatcca ggctaaaatc
     3601 gccgtgggtc accacgctat ccgggctaaa cggcagcagt ttatgcattt ctttccacac
     3661 ctgttccacc ggccagccgt tacgttcatc atcaaaatcg ctcgcatcca ccaggccgtt
     3721 gttcatacgg ctctgcgcct gggccagacg aaacacacga tcgctgttaa acgggcagtt
     3781 gcacaccgga atgctatgca gacgacgcag aaacacggcc agcgcatcca caatgttttc
     3841 gccgctatcc ggatattctt ccagcacctg aaacgcggtt ttgcccggaa tcgcggtggt
     3901 cagcagccac gcatcatccg gggtgcgaat aaaatgttta atggtcggca gcggcataaa
     3961 ttcggtcagc cagttcagac gcaccatttc atcggtcaca tcgttcgcca cgctgccttt
     4021 gccatgtttc agaaacagtt ccggcgcatc cggtttgcca tacagacgat aaatggtcgc
     4081 gccgctctga cccacgttat cacgcgccca tttatagcca tacagatccg catccatgtt
     4141 gctgttcaga cgcggacggc tacagctcgt ttcacgctga atatggctca taacacccct
     4201 tgtattactg tttatgtaag cagacagttt tattgttcat gatgatatat ttttatcttg
     4261 tgcaatgtaa catcagagat tttgagacac aa
//
