LOCUS       sequence-296924-        3800 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression vector for plants with medium-copy number origin of 
            replication.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3800)
  AUTHORS   Andreou , Andreas I., Nirkko, Jessica , Ochoa-Villarreal, Marisol , 
            Nakayama, Naomi
  TITLE     Mobius Assembly for Plant Systems highlights promoter-terminator 
            interaction in gene regulation
  JOURNAL   bioRxiv, March 31, 2021
REFERENCE   2  (bases 1 to 3800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv, 
            March 31, 2021"
COMMENT     Sequence Label: pLXMA L1A
FEATURES             Location/Qualifiers
     source          1..3800
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    133..157
                     /label=LB T-DNA repeat
                     /note="left border repeat from octopine Ach5 T-DNA"
     misc_feature    184..208
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        250..284
                     /label=BBa_J23106
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.47"
     RBS             293..304
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      993..1064
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1080..1107
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(1338..2153)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     2145..2164
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             complement(2279..2941)
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pBBR1 from Bordetella bronchiseptica"
                     /label=pBBR1 Rep
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     rep_origin      2942..3713
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
ORIGIN
        1 ccatcatcag ttcggtggtc ttccgacgaa caataaggcc gcaaatcgcg gcctttttta
       61 ttgataacaa aaccggctca gttctgcgta gaaaccaaca tgcaagctcc accgggtgca
      121 aagcggcagc ggcggcagga tatattcaat tgtaaatggc ttcatgtccg ggaaatctac
      181 tggtggcagg atatattgtg gtgtaaacaa tggagaaaaa gagaattcac ctgcatatgg
      241 agagagacct ttacggctag ctcagtccta ggtatagtgc tagctactag agaaagagga
      301 gaaatactaa atgtcgcact caaaacaagc actggcagat acgatgaaaa tgacgtggct
      361 gatggaaggt tcggtcaacg gtcacgcctt tacgattgaa ggcgaaggta ccggcaaacc
      421 gtatgaaggt aaacagagtg gcacctttcg tgttacgaaa ggcggtccgc tgccgtttgc
      481 gtttgatatt gtcgctccga ccctgaaata cggtttcaaa tgcttcatga aatacccggc
      541 ggatattccg gactacttta aactggcctt cccggaaggc ctgacgtacg atcgtaaaat
      601 tgcgtttgaa gacggcggtt gtgcgaccgc cacggtcgaa atgagcctga agggtaacac
      661 cctggtgcat aaaacgaact ttcagggcgg taatttcccg attgatggtc cggtgatgca
      721 aaaacgcacc ctgggctggg aaccgacctc tgaaaaaatg acgccgtgcg atggtattat
      781 caaaggcgac accatcatgt acctgatggt tgaaggcggt aaaacgctga aatgtcgtta
      841 tgaaaacaat taccgcgcca acaaaccagt gctgatgccg ccgagccact ttgtggatct
      901 gcgtctgacc cgcacgaatc tggataaaga aggtctggcg ttcaaactgg aagaatatgc
      961 tgttgcccgt gtgctggaag tgtaataagc ttcaaataaa acgaaaggct cagtcgaaag
     1021 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg
     1081 gctcaccttc gggtgggcct ttctgcgggt ctcacgctca gaatatgcag gtgcctgcag
     1141 gaattgttga ttttgtgatg actgatggca ggatatatgc ggttgtaatt catttttatt
     1201 gtctaaattt ctgtatttgt ttgtttgttc ggttgtaaat ttttttggaa gaacaagaaa
     1261 agaaaaaaca cccgttaggg tgtttttagt tagtgtggcg cgccgacttg cgacatgcgg
     1321 tcctttgcaa tcaactatta gaaaaattca tccagcatca gatgaaattg cagtttgttc
     1381 atatccggat tatcaatgcc atatttctga aacagacgtt tttgcaggct cgggctaaat
     1441 tcgcccaggc agttccacag aatggccaga tcctgataac gatccgcaat gcccacacgg
     1501 cccacatcaa tgcagccaat cagtttgcct tcatcgaaaa tcaggttatc caggctaaaa
     1561 tcgccgtggg tcaccacgct atccgggcta aacggcagca gtttatgcat ttctttccac
     1621 acctgttcca ccggccagcc gttacgttca tcatcaaaat cgctcgcatc caccaggccg
     1681 ttgttcatac ggctctgcgc ctgggccaga cgaaacacac gatcgctgtt aaacgggcag
     1741 ttgcacaccg gaatgctatg cagacgacgc agaaacacgg ccagcgcatc cacaatgttt
     1801 tcgccgctat ccggatattc ttccagcacc tgaaacgcgg ttttgcccgg aatcgcggtg
     1861 gtcagcagcc acgcatcatc cggggtgcga ataaaatgtt taatggtcgg cagcggcata
     1921 aattcggtca gccagttcag acgcaccatt tcatcggtca catcgttcgc cacgctgcct
     1981 ttgccatgtt tcagaaacag ttccggcgca tccggtttgc catacagacg ataaatggtc
     2041 gcgccgctct gacccacgtt atcacgcgcc catttatagc catacagatc cgcatccatg
     2101 ttgctgttca gacgcggacg gctacagctc gtttcacgct gaatatggct cataacaccc
     2161 cttgtattac tgtttatgta agcagacagt tttattgttc atgatgatat atttttatct
     2221 tgtgcaatgt aacatcagag attttgagac acaagatcgg attggcggtt atgcggttct
     2281 accggcgcgg cagcgttacc cgtgtcggcg gctccaacgg ctcgccatcg tccagaaaac
     2341 acggctcatc gggcatcggc aggcgctgct gcccgcgccg ttcccattcc tccgtttcgg
     2401 tcaaggctgg caggtctggt tccatgcccg gaatgccggg ctggctgggc ggctcctcgc
     2461 cggggccggt cggtagttgc tgctcgcccg gatacagggt cgggatgcgg cgcaggtcgc
     2521 catgccccaa cagcgattcg tcctggtcgt cgtgatcaac caccacggcg gcactgaaca
     2581 ccgacaggcg caactggtcg cggggctggc cccacgccac gcggtcattg accacgtagg
     2641 ccgacacggt gccggggccg ttgagcttca cgacggagat ccagcgctcg gccaccaagt
     2701 ccttgactgc gtattggacc gtccgcaaag aacgtccgat gagcttggaa agtgtcttct
     2761 ggctgaccac cacggcgttc tggtggccca tctgcgccac gaggtgatgc agcagcattg
     2821 ccgccgtggg tttcctcgca ataagcccgg cccacgcctc atgcgctttg cgttccgttt
     2881 gcacccagtg accgggcttg ttcttggctt gaatgccgat ttctctggac tgcgtggcca
     2941 tgcttatctc catgcggtag gggtgccgca cggttgcggc accatgcgca atcagctgca
     3001 acttttcggc agcgcgacaa caattatgcg ttgcgtaaaa gtggcagtca attacagatt
     3061 ttctttaacc tacgcaatga gctattgcgg ggggtgccgc aatgagctgt tgcgtacccc
     3121 ccttttttaa gttgttgatt tttaagtctt tcgcatttcg ccctatatct agttctttgg
     3181 tgcccaaaga agggcacccc tgcggggttc ccccacgcct tcggcgcggc tccccctccg
     3241 gcaaaaagtg gcccctccgg ggcttgttga tcgactgcgc ggccttcggc cttgcccaag
     3301 gtggcgctgc ccccttggaa cccccgcact cgccgccgtg aggctcgggg ggcaggcggg
     3361 cgggcttcgc ccttcgactg cccccactcg cataggcttg ggtcgttcca ggcgcgtcaa
     3421 ggccaagccg ctgcgcggtc gctgcgcgag ccttgacccg ccttccactt ggtgtccaac
     3481 cggcaagcga agcgcgcagg ccgcaggccg gaggcttttc cccagagaaa attaaaaaaa
     3541 ttgatggggc aaggccgcag gccgcgcagt tggagccggt gggtatgtgg tcgaaggctg
     3601 ggtagccggt gggcaatccc tgtggtcaag ctcgtgggca ggcgcagcct gtccatcagc
     3661 ttgtccagca gggttgtcca cgggccgagc gaagcgagcc agccggtggc cgctcgcggc
     3721 catcgtccac atatccacgg gctggcaagg gagcgcagcg accgcgcagg gcgaagcccg
     3781 gagagcaagc ccgtaggggg
//
