LOCUS       sequence-296973-        2108 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial expression plasmid for SARS-CoV-2 ORF8 protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2108)
  TITLE     SARS-CoV-2 protein E. coli expression constructs, tagged, for 
            protein expression and/or purification
REFERENCE   2  (bases 1 to 2108)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGBW-m4046811
FEATURES             Location/Qualifiers
     source          1..2108
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..25
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             40..62
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             433..453
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             454..483
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     rep_origin      604..1192
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1093..1112
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             1298..1957
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1364..1383)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      2028..2062
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     primer_bind     join(2090..2108,1)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2090..2108
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ggaattgtga gcggataaca attcccctct agaaataatt ttgtttaact ttaagaagga
       61 gactcttcta tgaaatttct tgttttctta ggaatcatca caactgtagc tgcatttcac
      121 caagaatgta gtttacagtc atgtactcaa catcaaccat atgtagttga tgacccgtgt
      181 cctattcact tctattctaa atggtatatt agagtaggag ctagaaaatc agcaccttta
      241 attgaattgt gcgtggatga ggctggttct aaatcaccca ttcagtacat cgatatcggt
      301 aattatacag tttcctgttt accttttaca attaattgcc aggaacctaa attgggtagt
      361 cttgtagtgc gttgttcgtt ctatgaagac tttttagagt atcatgacgt tcgtgttgtt
      421 ttagatttca tcgagaacct gtactttcaa agcgagcaga agcttatttc cgaagaagat
      481 ctctaaccgg cttatcggtc agtttcacct gatttacgta aaaacccgct tcggcgggtt
      541 tttgcttttg gaggggcaga aagatgaatg actgtccacg acgctatacc caaaagaaaa
      601 agcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
      661 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
      721 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
      781 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
      841 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
      901 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
      961 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     1021 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     1081 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     1141 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aattgatcgg
     1201 gcacgtaaga ggttccaact ttcaccataa tgaaataaga tcactaccgg gcgtattttt
     1261 tgagttatcg agattttcag gagctaagga agctaaaatg gagaaaaaaa tcactggata
     1321 taccaccgtt gatatatccc aatggcatcg taaagaacat tttgaggcat ttcagtcagt
     1381 tgctcaatgt acctataacc agaccgttca gctggatatt acggcctttt taaagaccgt
     1441 aaagaaaaat aagcacaagt tttatccggc ctttattcac attcttgccc gcctgatgaa
     1501 tgctcatccg gaatttcgta tggcaatgaa agacggtgag ctggtgatat gggatagtgt
     1561 tcacccttgt tacaccgttt tccatgagca aactgaaacg ttttcatcgc tctggagtga
     1621 ataccacgac gatttccggc agtttctaca catatattcg caagatgtgg cgtgttacgg
     1681 tgaaaacctg gcctatttcc ctaaagggtt tattgagaat atgtttttcg tttcagccaa
     1741 tccctgggtg agtttcacca gttttgattt aaacgtggcc aatatggaca acttcttcgc
     1801 ccccgttttc accatgggca aatattatac gcaaggcgac aaggtgctga tgccgctggc
     1861 gattcaggtt catcatgccg tttgtgatgg cttccatgtc ggcagaatgc ttaatgaatt
     1921 acaacagtac tgcgatgagt ggcagggcgg ggcgtaattt gatatcgagc tcgcttggac
     1981 tcctgttgat agatccagta atgacctcag aactccatct ggatttgttc agaacgctcg
     2041 gttgccgccg ggcgtttttt attggtcatc gatctcgatc ccgcgaaatt aatacgactc
     2101 actatagg
//
