LOCUS       sequence-297027-       11613 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mApple expression regulated by the A. thaliana UBQ10 promoter, 
            mCitrine expression regulated by the A. thaliana CURT1D promoter. 
            nourseothricin acetyl transferase selectable marker (nat).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11613)
  TITLE     Quantitative comparison of specialised leaf promoters
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11613)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: GS1.2/CURT1D
FEATURES             Location/Qualifiers
     source          1..11613
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             664..1374
                     /codon_start=1
                     /product="photostable monomeric derivative of DsRed (Shaner
                     et al., 2008)"
                     /label=mApple
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
                     FQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNF
                     EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALK
                     SEIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     1077..1096
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     protein_bind    complement(1377..1401)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    1721..1745
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             3076..3705
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             4134..5207
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      5273..5467
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(5703..5725)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    5811..5951
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     5866..5885
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(5966..5983)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6137..6725)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6217..6236)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(6971..7762)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_feature    8283..8307
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(8400..8652)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(8675..9247)
                     /codon_start=1
                     /gene="Streptomyces noursei nat1"
                     /product="nourseothricin acetyltransferase"
                     /label=NrsR
                     /note="confers resistance to nourseothricin"
                     /translation="MGTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDGF
                     TLREVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGWNR
                     RLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRMGFT
                     LCGLDTALYDGTASDGEQALYMSMPCP"
     promoter        complement(9289..9468)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     terminator      complement(9578..9830)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    9833..9857
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(join(9872..10585,10586..10588,10589..10591))
                     /codon_start=1
                     /product="enhanced YFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(9917..9938)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     10266..10285
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     10525..10546
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 tcgacgagtc agtaataaac ggcgtcaaag tggttgcagc cggcacacac gagtcgtgtt
       61 tatcaactca aagcacaaat acttttcctc aacctaaaaa taaggcaatt agccaaaaac
      121 aactttgcgt gtaaacaacg ctcaatacac gtgtcatttt attattagct attgcttcac
      181 cgccttagct ttctcgtgac ctagtcgtcc tcgtcttttc ttcttcttct tctataaaac
      241 aatacccaaa gagctcttct tcttcacaat tcagatttca atttctcaaa atcttaaaaa
      301 ctttctctca attctctcta ccgtgatcaa ggtaaatttc tgtgttcctt attctctcaa
      361 aatcttcgat tttgttttcg ttcgatccca atttcgtata tgttctttgg tttagattct
      421 gttaatctta gatcgaagac gattttctgg gtttgatcgt tagatatcat cttaattctc
      481 gattagggtt tcatagatat catccgattt gttcaaataa tttgagtttt gtcgaataat
      541 tactcttcga tttgtgattt ctatctagat ctggtgttag tttctagttt gtgcgatcga
      601 atttgtcgat taatctgagt ttttctgatt aacaggcggc cgcactagtg atatcacaag
      661 tttatggtga gcaagggcga ggagaataac atggccatca tcaaggagtt catgcgcttc
      721 aaggtgcaca tggagggctc cgtgaacggc cacgagttcg agatcgaggg cgagggcgag
      781 ggccgcccct acgaggcctt tcagaccgct aagctgaagg tgaccaaggg tggccccctg
      841 cccttcgcct gggacatcct gtcccctcag ttcatgtacg gctccaaggt ctacattaag
      901 cacccagccg acatccccga ctacttcaag ctgtccttcc ccgagggctt caggtgggag
      961 cgcgtgatga acttcgagga cggcggcatt attcacgtta accaggactc ctccctgcag
     1021 gacggcgtgt tcatctacaa ggtgaagctg cgcggcacca acttcccctc cgacggcccc
     1081 gtaatgcaga agaagaccat gggctgggag gcctccgagg agcggatgta ccccgaggac
     1141 ggcgccctga agagcgagat caagaagagg ctgaagctga aggacggcgg ccactacgcc
     1201 gccgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg cgcctacatc
     1261 gtcgacatca agttggacat cgtgtcccac aacgaggact acaccatcgt ggaacagtac
     1321 gaacgcgccg agggccgcca ctccaccggc ggcatggacg agctgtacaa gtaatcgttc
     1381 agctttcttg tacaaagtgg tgatatcccg cggccatgct agagtccgca aaaatcacca
     1441 gtctctctct acaaatctat ctctctctat ttttctccag aataatgtgt gagtagttcc
     1501 cagataaggg aattagggtt cttatagggt ttcgctcatg tgttgagcat ataagaaacc
     1561 cttagtatgt atttgtattt gtaaaatact tctatcaata aaatttctaa ttcctaaaac
     1621 caaaatccag tgacctgcag gcatgcgacg tcgggcccaa gcttagcttg agcttggatc
     1681 agattgtcgt ttcccgcctt cagtttaaac tatcagtgtt tgacaggata tattggcggg
     1741 taaacctaag agaaaagagc gtttattaga ataatcggat atttaaaagg gcgtgaaaag
     1801 gtttatccgt tcgtccattt gtatgtgcat gccaaccaca gggttcccct cgggatcaaa
     1861 gtactttaaa gtactttaaa gtactttaaa gtactttgat ccaacccctc cgctgctata
     1921 gtgcagtcgg cttctgacgt tcagtgcagc cgtcttctga aaacgacatg tcgcacaagt
     1981 cctaagttac gcgacaggct gccgccctgc ccttttcctg gcgttttctt gtcgcgtgtt
     2041 ttagtcgcat aaagtagaat acttgcgact agaaccggag acattacgcc atgaacaaga
     2101 gcgccgccgc tggcctgctg ggctatgccc gcgtcagcac cgacgaccag gacttgacca
     2161 accaacgggc cgaactgcac gcggccggct gcaccaagct gttttccgag aagatcaccg
     2221 gcaccaggcg cgaccgcccg gagctggcca ggatgcttga ccacctacgc cctggcgacg
     2281 ttgtgacagt gaccaggcta gaccgcctgg cccgcagcac ccgcgaccta ctggacattg
     2341 ccgagcgcat ccaggaggcc ggcgcgggcc tgcgtagcct ggcagagccg tgggccgaca
     2401 ccaccacgcc ggccggccgc atggtgttga ccgtgttcgc cggcattgcc gagttcgagc
     2461 gttccctaat catcgaccgc acccggagcg ggcgcgaggc cgccaaggcc cgaggcgtga
     2521 agtttggccc ccgccctacc ctcaccccgg cacagatcgc gcacgcccgc gagctgatcg
     2581 accaggaagg ccgcaccgtg aaagaggcgg ctgcactgct tggcgtgcat cgctcgaccc
     2641 tgtaccgcgc acttgagcgc agcgaggaag tgacgcccac cgaggccagg cggcgcggtg
     2701 ccttccgtga ggacgcattg accgaggccg acgccctggc ggccgccgag aatgaacgcc
     2761 aagaggaaca agcatgaaac cgcaccagga cggccaggac gaaccgtttt tcattaccga
     2821 agagatcgag gcggagatga tcgcggccgg gtacgtgttc gagccgcccg cgcacgtctc
     2881 aaccgtgcgg ctgcatgaaa tcctggccgg tttgtctgat gccaagctgg cggcctggcc
     2941 ggccagcttg gccgctgaag aaaccgagcg ccgccgtcta aaaaggtgat gtgtatttga
     3001 gtaaaacagc ttgcgtcatg cggtcgctgc gtatatgatg cgatgagtaa ataaacaaat
     3061 acgcaagggg aacgcatgaa ggttatcgct gtacttaacc agaaaggcgg gtcaggcaag
     3121 acgaccatcg caacccatct agcccgcgcc ctgcaactcg ccggggccga tgttctgtta
     3181 gtcgattccg atccccaggg cagtgcccgc gattgggcgg ccgtgcggga agatcaaccg
     3241 ctaaccgttg tcggcatcga ccgcccgacg attgaccgcg acgtgaaggc catcggccgg
     3301 cgcgacttcg tagtgatcga cggagcgccc caggcggcgg acttggctgt gtccgcgatc
     3361 aaggcagccg acttcgtgct gattccggtg cagccaagcc cttacgacat atgggccacc
     3421 gccgacctgg tggagctggt taagcagcgc attgaggtca cggatggaag gctacaagcg
     3481 gcctttgtcg tgtcgcgggc gatcaaaggc acgcgcatcg gcggtgaggt tgccgaggcg
     3541 ctggccgggt acgagctgcc cattcttgag tcccgtatca cgcagcgcgt gagctaccca
     3601 ggcactgccg ccgccggcac aaccgttctt gaatcagaac ccgagggcga cgctgcccgc
     3661 gaggtccagg cgctggccgc tgaaattaaa tcaaaactca tttgagttaa tgaggtaaag
     3721 agaaaatgag caaaagcaca aacacgctaa gtgccggccg tccgagcgca cgcagcagca
     3781 aggctgcaac gttggccagc ctggcagaca cgccagccat gaagcgggtc aactttcagt
     3841 tgccggcgga ggatcacacc aagctgaaga tgtacgcggt acgccaaggc aagaccatta
     3901 ccgagctgct atctgaatac atcgcgcagc taccagagta aatgagcaaa tgaataaatg
     3961 agtagatgaa ttttagcggc taaaggaggc ggcatggaaa atcaagaaca accaggcacc
     4021 gacgccgtgg aatgccccat gtgtggagga acgggcggtt ggccaggcgt aagcggctgg
     4081 gttgtctgcc ggccctgcaa tggcactgga acccccaagc ccgaggaatc ggcgtgagcg
     4141 gtcgcaaacc atccggcccg gtacaaatcg gcgcggcgct gggtgatgac ctggtggaga
     4201 agttgaaggc cgcgcaggcc gcccagcggc aacgcatcga ggcagaagca cgccccggtg
     4261 aatcgtggca agcggccgct gatcgaatcc gcaaagaatc ccggcaaccg ccggcagccg
     4321 gtgcgccgtc gattaggaag ccgcccaagg gcgacgagca accagatttt ttcgttccga
     4381 tgctctatga cgtgggcacc cgcgatagtc gcagcatcat ggacgtggcc gttttccgtc
     4441 tgtcgaagcg tgaccgacga gctggcgagg tgatccgcta cgagcttcca gacgggcacg
     4501 tagaggtttc cgcagggccg gccggcatgg ccagtgtgtg ggattacgac ctggtactga
     4561 tggcggtttc ccatctaacc gaatccatga accgataccg ggaagggaag ggagacaagc
     4621 ccggccgcgt gttccgtcca cacgttgcgg acgtactcaa gttctgccgg cgagccgatg
     4681 gcggaaagca gaaagacgac ctggtagaaa cctgcattcg gttaaacacc acgcacgttg
     4741 ccatgcagcg tacgaagaag gccaagaacg gccgcctggt gacggtatcc gagggtgaag
     4801 ccttgattag ccgctacaag atcgtaaaga gcgaaaccgg gcggccggag tacatcgaga
     4861 tcgagctagc tgattggatg taccgcgaga tcacagaagg caagaacccg gacgtgctga
     4921 cggttcaccc cgattacttt ttgatcgatc ccggcatcgg ccgttttctc taccgcctgg
     4981 cacgccgcgc cgcaggcaag gcagaagcca gatggttgtt caagacgatc tacgaacgca
     5041 gtggcagcgc cggagagttc aagaagttct gtttcaccgt gcgcaagctg atcgggtcaa
     5101 atgacctgcc ggagtacgat ttgaaggagg aggcggggca ggctggcccg atcctagtca
     5161 tgcgctaccg caacctgatc gagggcgaag catccgccgg ttcctaatgt acggagcaga
     5221 tgctagggca aattgcccta gcaggggaaa aaggtcgaaa aggtctcttt cctgtggata
     5281 gcacgtacat tgggaaccca aagccgtaca ttgggaaccg gaacccgtac attgggaacc
     5341 caaagccgta cattgggaac cggtcacaca tgtaagtgac tgatataaaa gagaaaaaag
     5401 gcgatttttc cgcctaaaac tctttaaaac ttattaaaac tcttaaaacc cgcctggcct
     5461 gtgcataact gtctggccag cgcacagccg aagagctgca aaaagcgcct acccttcggt
     5521 cgctgcgctc cctacgcccc gccgcttcgc gtcggcctat cgcggccgct ggccgctcaa
     5581 aaatggctgg cctacggcca ggcaatctac cagggcgcgg acaagccgcg ccgtcgccac
     5641 tcgaccgccg gcgcccacat caaggcaccc tgcctcgcgc gtttcggtga tgacggtgaa
     5701 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg
     5761 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg
     5821 acccagtcac gtagcgatag cggagtgtat actggcttaa ctatgcggca tcagagcaga
     5881 ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat
     5941 accgcatcag gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     6001 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     6061 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     6121 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     6181 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     6241 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     6301 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     6361 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     6421 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     6481 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     6541 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
     6601 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     6661 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     6721 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     6781 gttaagggat tttggtcatg catgatatat ctcccaattt gtgtagggct tattatgcac
     6841 gcttaaaaat aataaaagca gacttgacct gatagtttgg ctgtgagcaa ttatgtgctt
     6901 agtgcatcta acgcttgagt taagccgcgc cgcgaagcgg cgtcggcttg aacgaatttc
     6961 tagctagaca ttatttgccg actaccttgg tgatctcgcc tttcacgtag tggacaaatt
     7021 cttccaactg atctgcgcgc gaggccaagc gatcttcttc ttgtccaaga taagcctgtc
     7081 tagcttcaag tatgacgggc tgatactggg ccggcaggcg ctccattgcc cagtcggcag
     7141 cgacatcctt cggcgcgatt ttgccggtta ctgcgctgta ccaaatgcgg gacaacgtaa
     7201 gcactacatt tcgctcatcg ccagcccagt cgggcggcga gttccatagc gttaaggttt
     7261 catttagcgc ctcaaataga tcctgttcag gaaccggatc aaagagttcc tccgccgctg
     7321 gacctaccaa ggcaacgcta tgttctcttg cttttgtcag caagatagcc agatcaatgt
     7381 cgatcgtggc tggctcgaag atacctgcaa gaatgtcatt gcgctgccat tctccaaatt
     7441 gcagttcgcg cttagctgga taacgccacg gaatgatgtc gtcgtgcaca acaatggtga
     7501 cttctacagc gcggagaatc tcgctctctc caggggaagc cgaagtttcc aaaaggtcgt
     7561 tgatcaaagc tcgccgcgtt gtttcatcaa gccttacggt caccgtaacc agcaaatcaa
     7621 tatcactgtg tggcttcagg ccgccatcca ctgcggagcc gtacaaatgt acggccagca
     7681 acgtcggttc gagatggcgc tcgatgacgc caactacctc tgatagttga gtcgatactt
     7741 cggcgatcac cgcttccccc atgatgttta actttgtttt agggcgactg ccctgctgcg
     7801 taacatcgtt gctgctccat aacatcaaac atcgacccac ggcgtaacgc gcttgctgct
     7861 tggatgcccg aggcatagac tgtaccccaa aaaaacagtc ataacaagcc atgaaaaccg
     7921 ccactgcgcc gttaccaccg ctgcgttcgg tcaaggttct ggaccagttg cgtgagcgca
     7981 tacgctactt gcattacagc ttacgaaccg aacaggctta tgtccactgg gttcgtgccc
     8041 gaattgatca caggcagcaa cgctctgtca tcgttacaat caacatgcta ccctccgcga
     8101 gatcatccgt gtttcaaacc cggcagctta gttgccgttc ttccgaatag catcggtaac
     8161 atgagcaaag tctgccgcct tacaacggct ctcccgctga cgccgtcccg gactgatggg
     8221 ctgcctgtat cgagtggtga ttttgtgccg agctgccggt cggggagctg ttggctggct
     8281 ggtggcagga tatattgtgg tgtaaacaaa ttgacgctta gacaacttaa taacacattg
     8341 cggacgtttt taatgtactg aattaacgcc gaattgaatt atcagcttgc atgccggtcg
     8401 atctagtaac atagatgaca ccgcgcgcga taatttatcc tagtttgcgc gctatatttt
     8461 gttttctatc gcgtattaaa tgtataattg cgggactcta atcataaaaa cccatctcat
     8521 aaataacgtc atgcattaca tgttaattat tacatgctta acgtaattca acagaaatta
     8581 tatgataatc atcgcaagac cggcaacagg attcaatctt aagaaacttt attgccaaat
     8641 gtttgaacga tctgcttgac tctaggggtc atcattaggg gcagggcatg ctcatgtaga
     8701 gcgcctgctc gccgtccgag gcggtgccgt cgtacagggc ggtgtccagg ccgcagaggg
     8761 tgaaccccat ccgccggtac gcgtggatcg ccggtgcgtt gacgttggtg acctccagcc
     8821 agaggtgccc ggcaccccgc tcgcgggcga actccgtcgc gagccccatc aacgcgcgcc
     8881 cgaccccgtg cccccggtgc tccggggcga cctcgatgtc ctcgacggtc agccggcggt
     8941 tccagccgga gtacgagacg accacgaagc ccgccaggtc gccgtcgtcc ccgtacgcga
     9001 cgaacgtccg agagtccggg tcgccgtcct ccccgtcgtc cgattcgtcg tccgattcgt
     9061 cgtcggggaa caccttggtc aggggcgggt ccaccggcac ctcccgcagg gtgaagccgt
     9121 ccccggtggc ggtgacgcgg aagacggtgt cggtggtgaa ggacccatcc agtgcctcga
     9181 tggcctcggc gtcccccggg acactggtgc ggtaccggta agccgtgtcg tcaagagtgg
     9241 tacccatggt agatcccctc gatcgagttg agagtgaata tgagactcta attggatacc
     9301 gaggggaatt tatggaacgt cagtggagca tttttgacaa gaaatatttg ctagctgata
     9361 gtgaccttag gcgacttttg aacgcgcaat aatggtttct gacgtatgtg cttagctcat
     9421 taaactccag aaacccgcgg ctcagtggct ccttcaacgt tgcggttctg tcagttccaa
     9481 acgtaaaacg gcttgtcccg cgtcatcggc gggggtcata acgtgactcc cttaattctc
     9541 cgctcatgat tgataattcg agctctccca tatggtcgat ctagtaacat agatgacacc
     9601 gcgcgcgata atttatccta gtttgcgcgc tatattttgt tttctatcgc gtattaaatg
     9661 tataattgcg ggactctaat cataaaaacc catctcataa ataacgtcat gcattacatg
     9721 ttaattatta catgcttaac gtaattcaac agaaattata tgataatcat cgcaagaccg
     9781 gcaacaggat tcaatcttaa gaaactttat tgccaaatgt ttgaacgatc tgccaacttt
     9841 gtacaagaaa gctgggtcgg cgcgcccacc cttacttgta cagctcgtcc atgccgagag
     9901 tgatcccggc ggcggtcacg aactccagca ggaccatgtg atcgcgcttc tcgttggggt
     9961 ctttgctcag cttggactgg tagctcaggt agtggttgtc gggcagcagc acggggccgt
    10021 cgccgatggg ggtgttctgc tggtagtggt cggcgagctg cacgctgccg tcctcgatgt
    10081 tgtggcggat cttgaagttc accttgatgc cgttcttctg cttgtcggcc atgatataga
    10141 cgttgtggct gttgtagttg tactccagct tgtgccccag gatgttgccg tcctccttga
    10201 agtcgatgcc cttcagctcg atgcggttca ccagggtgtc gccctcgaac ttcacctcgg
    10261 cgcgggtctt gtagttgccg tcgtccttga agaagatggt gcgctcctgg acgtagcctt
    10321 cgggcatggc ggacttgaag aagtcgtgct gcttcatgtg gtcggggtag cgggcgaagc
    10381 acatcaggcc gtagccgaag gtggtcacga gggtgggcca gggcacgggc agcttgccgg
    10441 tggtgcagat gaacttcagg gtcagcttgc cgtaggtggc atcgccctcg ccctcgccgg
    10501 acacgctgaa cttgtggccg tttacgtcgc cgtccagctc gaccaggatg ggcaccaccc
    10561 cggtgaacag ctcctcgccc ttgctcacca ttttcgtacg gatgcatcaa agtcatttca
    10621 tctcctgaaa aaaactgtca ttttataaac aaggcttcta gggttttgga taatgggccg
    10681 ttgttaatac ttggttcggc ccaaacatat aaaacttcaa aaaccggtct gttataaagt
    10741 accactttgg attaccttag cgccaatttc tttctttctt ctttggattt ggaatagtca
    10801 aagtattata ctttacttaa aaacgatagc tctctctaat cgcatgagga cagccaactc
    10861 caaaatctca ttttcatggt tcatacggtt ttatactaat ccttatatgt cacacaaaat
    10921 taaaatcata tgttttctat aatatatccg ccaatttcat tctgatgctt ttggcaaaat
    10981 ttgtcatggg agctaatgta catgtgttga actgtttaca gtcacttgag ttggaaatta
    11041 cagggccaag ttaacccgat cgagaagcat tgcttgctca gtcaattcac attgctatct
    11101 aactcctgca acttttgagc catttcagag ctatgtccag cccgcaaact tctggcaagc
    11161 gaccgtatag ctctagagat caactctttc ttctgcttcc cacccgaagt gcttgtgccg
    11221 cttgatccag aaacattatc ttcctctttc aatgtcacac acaatcctct caaccactcc
    11281 tctagaaatg aggctttctg tcgggtttca tgcgacacca caagacaata cgagtcacta
    11341 atcctttaaa atcaagcgat gaaaacagtt gtattaacaa taatagctgc cagtttctta
    11401 ctcttataag aaattctgac aacgcttcga aatgacagct tgaaactagg ctcaggagat
    11461 gcagcatggt tgacgttaac tgcaacgagc aactgcgttt aagataaatc attcatcaat
    11521 acctaaaatt acgccaacat catgttacct gattctcaag cgacctctta tatttgaaat
    11581 ttgctggagt cgcattgctc atcaatttgt tgg
//
