LOCUS       sequence-297053-        8391 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express MCART1 in S. cerevisiae.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8391)
  AUTHORS   Kory N, Uit de Bos J, van der Rijt S, Jankovic N, Gura M, Arp N, 
            Pena IA, Prakash G, Chan SH, Kunchok T, Lewis CA, Sabatini DM
  TITLE     MCART1/SLC25A51 is required for mitochondrial NAD transport.
  JOURNAL   Sci Adv. 2020 Oct 21;6(43):eabe5310. doi: 10.1126/sciadv.abe5310. 
            Print 2020 Oct.
  PUBMED    33087354
REFERENCE   2  (bases 1 to 8391)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2020-10-21"; volume: "6(43):eabe5310. doi"; pages: " 
            10.1126/sciadv.abe5310. Print 2020 Oct"
COMMENT     Sequence Label: YCplac33-NAT_MCART1
FEATURES             Location/Qualifiers
     source          1..8391
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(2021..2038)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2021..2037)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2030..2052)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    2794..3956
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN4 centromere fused to the 
                     autonomously replicating sequence ARS1/ARS416"
     rep_origin      3119..3956
                     /label=ARS1
                     /note="S. cerevisiae autonomously replicating sequence 
                     ARS1/ARS416"
     promoter        4896..5238
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     terminator      5818..6015
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(6184..6202)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6270..6374
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6375..6443,6444..7235)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6593..6612)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7406..7994
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7895..7914
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8148..8165
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8282..8303
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8318..8323,8324..8341,8342..8348)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8356..8372
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8361..8383
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     join(8380..8391,1..5)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     join(8380..8391,1..5)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 atgaccatga ttacgccaag cttgcatgcc tgcaggtcga ctctagagga tccgtcacca
       61 acgcagagtc catgccgatg ccttgctgct caagcacact gaaatacgcc tctagttgtg
      121 tctcaatatc tgtctttaga accatcaatt ccgacagttt gaagctgtct atttgactaa
      181 ttcttgatgt taggctcgga tctatcttaa cattggccaa caatttactt aattcttctt
      241 cctccattgt agttcgttct tattctaatt cttgctcctt tttactctgg tgataacttt
      301 tctatctctc aatgctgaac attttttttc cctttattat ccaatattcc aaccgttata
      361 aagtaagcct tatcgaacac aatccacggc gcacgcaagg gacagcaaat ttcgagaatt
      421 aatgaatagc cagtagtaag atccgtattg ataaaggtca tagatcccaa cctttatgtg
      481 agccagcccg aagggccctt taaatgactc tcataatatg gtccaatagc accaatttct
      541 aggggttacc caccggccta gtgcttaatc gaattgtgaa aagaatctct actacagact
      601 gccaaccgta atagtagtga gatcaacact gaaggcagca gtctttagca caatgatgga
      661 tagcgaagcc cacgagaagc gtccgcccat attgacaagt tccaaacagg acatcagccc
      721 acatatcacg aatgtaggag agatgaagca ttacttatgt ggctgctgtg ccgctttcaa
      781 caacgtagcc atcacctttc ccatacagaa agtcctgttc aggcaacagc tatacggtat
      841 taaaacgaga gatgccatct tgcagcttag aagagacgga tttaggaact tatatagggg
      901 aatcttgcca ccattgatgc aaaagaccac gacgttggcg ttaatgttcg gactatatga
      961 ggatcttagc tgcttacttc acaagcatgt cagtgcaccg gagttcgcta cgtcaggtgt
     1021 ggccgcagtc cttgctggta cgacggaggc cattttcacg ccgttggaaa gagtacagac
     1081 attgcttcag gatcataagc atcacgacaa attcactaat acatatcagg catttaaagc
     1141 tctgaagtgc cacggaatcg gagaatacta tagaggacta gtgcccatcc tgtttaggaa
     1201 cggtctgtct aacgtattgt tcttcggcct acgtgggcca atcaaagaac acttgccgac
     1261 agctacgact cattctgccc acctagtaaa tgactttata tgcggggggc ttttgggggc
     1321 tatgcttggc tttctatttt ttccgataaa tgtggtaaaa actaggatac agagtcagat
     1381 cggaggtgaa tttcagagtt ttcccaaggt cttccagaaa atctggctag aacgtgacag
     1441 aaagctgatt aatttatttc gtggagctca cttgaactac cacaggtctc taattagttg
     1501 gggcataatt aatgccacgt atgaattttt gttgaaggtg atatgaagcc ttaaaattca
     1561 cacacgccac acattgagtg tgctatgaat atgcctgcgc taatatttta ggtattacag
     1621 cttgtaaata taccaaataa ggtaagcctc tcttttccta atactaaaag gatttgcatc
     1681 gaacagccca ccaaatgctt tgcgcacaca ttatatacac ccacatgatt ctatagacaa
     1741 aatatactta tacattttca tagtcttgac aatattctac attggccgtt ttcttggcac
     1801 tattttagct tatccttacc aaaatagtct cttttttaaa tactattttt gaagcggaaa
     1861 taggtgcgaa caaatgaatg gcttcaattt agtgcgataa agacgtaaag cataaaccag
     1921 tgaagatcat agcactaaga aggcgcctgt actgttcctc gtgttgatac tcatttcctt
     1981 atttattttc ctcaagaaat tctatccagg aaaggaattc actggccgtc gttttacaac
     2041 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
     2101 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
     2161 gcctgaatgg cgaatggcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt
     2221 cacaccgcat aatcgctggg ccattctcat gaagaatatc ttgaatttat tgtcatatta
     2281 ctagttggtg tggaagtcct aatatcggtg atcaatatag tggttgacat gctggctagt
     2341 caacattgag ccttttgatc atgcaaatat attacggtat tttacaatca aatatcaaac
     2401 ttaactattg actttataac ttatttaggt ggtaacattc ttataaaaaa gaaaaaaatt
     2461 actgcaaaac agtactagct tttaacttgt atcctaggtt atctatgctg tctcaccata
     2521 gagaatatta cctatttcag aatgtatgtc catgattcgc cgggtaaata catataatac
     2581 acaaatctgg cttaataaag tctataatat atctcataaa gaagtgctaa attggctagt
     2641 gctatatatt tttaagaaaa tttcttttga ctaagtccat atcgactttg taaaagttca
     2701 cattagcata catatattac acgagccaga aatagtaact tttgcctaaa tcacaaattg
     2761 caaaatttaa ttgcttgcaa aaggtcacat gcttataatc aactttttta aaaatttaaa
     2821 atactttttt attttttatt tttaaacata aatgaaataa tttatttatt gtttatgatt
     2881 accgaaacat aaaacctgct caagaaaaag aaactgtttt gtccttggaa aaaaagcact
     2941 acctaggagc ggccaaaatg ccgaggcttt catagcttaa actctttaca gaaaataggc
     3001 attatagatc agttcgagtt ttcttattct tccttccggt tttatcgtca cagttttaca
     3061 gtaaataagt atcacctctt agagttcgat gataagctgt caaacatgag aattaattcc
     3121 acatgttaaa atagtgaagg agcatgttcg gcacacagtg gaccgaacgt ggggtaagtg
     3181 cactagggtc cggttaaacg gatctcgcat tgatgaggca acgctaatta tcaacatata
     3241 gattgttatc tatctgcatg aacacgaaat ctttacttga cgacttgagg ctgatggtgt
     3301 ttatgcaaag aaaccactgt gtttaatatg tgtcactgtt tgatattact gtcagcgtag
     3361 aagataatag taaaagcggt taataagtgt atttgagata agtgtgataa agtttttaca
     3421 gcgaaaagac gataaataca agaaaatgat tacgaggata cggagagagg tatgtacatg
     3481 tgtatttata tactaagctg ccggcggttg tttgcaagac cgagaaaagg ctagcaagaa
     3541 tcgggtcatt gtagcgtatg cgcctgtgaa cattctcttc aacaagtttg attccattgc
     3601 ggtgaaatgg taaaagtcaa ccccctgcga tgtatatttt cctgtacaat caatcaaaaa
     3661 gccaaatgat ttagcattat ctttacatct tgttatttta cagattttat gtttagatct
     3721 tttatgcttg cttttcaaaa ggcttgcagg caagtgcaca aacaatactt aaataaatac
     3781 tactcagtaa taacctattt cttagcattt ttgacgaaat ttgctatttt gttagagtct
     3841 tttacaccat ttgtctccac acctccgctt acatcaacac caataacgcc atttaatcta
     3901 agcgcatcac caacattttc tggcgtcagt ccaccagcta acataaaatg taagctctcg
     3961 gggctctctt gccttccaac ccagtcagaa atcgagttcc aatccaaaag ttcacctgtc
     4021 ccacctgctt ctgaatcaaa caagggaata aacgaatgag gtttctgtga agctgcactg
     4081 agtagtatgt tgcagtcttt tggaaatacg agtcttttaa taactggcaa accgaggaac
     4141 tcttggtatt cttgccacga ctcatctcca tgcagttgga cgatcgatga taagctgtca
     4201 aacatgagaa ttgggtaata actgatataa ttaaattgaa gctctaattt gtgagtttag
     4261 tatacatgca tttacttata atacagtttt ttagttttgc tggccgcatc ttctcaaata
     4321 tgcttcccag cctgcttttc tgtaacgttc accctctacc ttagcatccc ttccctttgc
     4381 aaatagtcct cttccaacaa taataatgtc agatcctgta gagaccacat catccacggt
     4441 tctatactgt tgacccaatg cgtctccctt gtcatctaaa cccacaccgg gtgtcataat
     4501 caaccaatcg taaccttcat ctcttccacc catgtctctt tgagcaataa agccgataac
     4561 aaaatctttg tcgctcttcg caatgtcaac agtaccctta gtatattctc cagtagatag
     4621 ggagcccttg catgacaatt ctgctaacat caaaaggcct ctaggttcct ttgttacttc
     4681 ttctgccgcc tgcttcaaac cgctaacaat acctgggccc accacaccgt gtgcattcgt
     4741 aatgtctgcc cattctgcta ttctgtatac acccgcagag tactgcaatt tgactgtatt
     4801 accaatgtca gcaaattttc tgtcttcgaa gagtaaaaaa ttgtacttgg cggataatgc
     4861 ctttagcggc ttaactgtgc cctcgcccgg ccagcgacat ggaggcccag aataccctcc
     4921 ttgacagtct tgacgtgcgc agctcagggg catgatgtga ctgtcgcccg tacatttagc
     4981 ccatacatcc ccatgtataa tcatttgcat ccatacattt tgatggccgc acggcgcgaa
     5041 gcaaaaatta cggctcctcg ctgcagacct gcgagcaggg aaacgctccc ctcacagacg
     5101 cgttgaattg tccccacgcc gcgcccctgt agagaaatat aaaaggttag gatttgccac
     5161 tgaggttctt ctttcatata cttcctttta aaatcttgct aggatacagt tctcacatca
     5221 catccgaaca taaacaacta tgggcacaac cttggatgat actgcctaca ggtacagaac
     5281 ctctgtgccg ggagacgctg aggcaataga ggcactagat ggctccttca caacggacac
     5341 cgtttttaga gttactgcca cgggagatgg gttcacttta cgtgaagtcc cggttgaccc
     5401 tccattgaca aaagtctttc cagacgatga gtctgacgac gaatcagacg acggggaaga
     5461 tggagatcca gactctagaa cttttgttgc ttatggtgat gatggcgatt tggccgggtt
     5521 cgtcgtcgtg agctactctg gttggaatag gaggttgacg gtcgaggaca tcgaggttgc
     5581 acctgagcat agaggacatg gggtgggcag agccttgatg ggcctggcta cagaatttgc
     5641 tcgtgaacgt ggggcggggc atctatggct tgaagtcaca aatgtcaatg ctcccgcaat
     5701 acatgcatat agacgtatgg gttttaccct gtgcgggctt gatacagctt tgtacgatgg
     5761 aaccgctagt gacggtgaac aagccttata tatgtccatg ccttgccctt aatcagtact
     5821 gacaataaaa agattcttgt tttcaagaac ttgtcatttg tatagttttt ttatattgta
     5881 gttgttctat tttaatcaaa tgttagcgtg atttatattt tttttcgcct cgacatcatc
     5941 tgcccagatg cgaagttaag tgcgcagaaa gtaatatcat gcgtcaatcg tatgtgaatg
     6001 ctggtcgcta tactgctgtc gattccatat gcgtatatat accaatctaa gtctgtgctc
     6061 cttccttcgt tcttccttct gttcggagat taccgaatca aaaaaatttc aaggaaaccg
     6121 aaatcaaaaa aaagaataaa aaaaaaatga tgaattgaaa agctaattct tgaagacgaa
     6181 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
     6241 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     6301 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     6361 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     6421 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     6481 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     6541 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     6601 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     6661 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     6721 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     6781 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     6841 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     6901 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     6961 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     7021 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     7081 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     7141 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     7201 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     7261 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     7321 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     7381 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     7441 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     7501 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     7561 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     7621 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     7681 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     7741 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     7801 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     7861 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     7921 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     7981 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     8041 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     8101 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     8161 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     8221 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
     8281 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
     8341 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc t
//
