LOCUS       sequence-297079-        8815 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  V5-His-tagged gene expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8815)
  AUTHORS   Yadav L, Pietila E, Ohman T, Liu X, Mahato AK, Sidorova Y, Lehti K, 
            Saarma M, Varjosalo M
  TITLE     PTPRA Phosphatase Regulates GDNF-Dependent RET Signaling and 
            Inhibits the RET Mutant MEN2A Oncogenic Potential.
  JOURNAL   iScience. 2020 Feb 21;23(2):100871. doi: 10.1016/j.isci.2020.100871.
            Epub 2020 Jan 31.
  PUBMED    32062451
REFERENCE   2  (bases 1 to 8815)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "iScience.";
            date: "2020-02-21"; volume: "23(2):100871. doi"; pages: " 
            10.1016/j.isci.2020.100871. Epub 2020 Jan 31"
COMMENT     Sequence Label: RET_KD-V5
FEATURES             Location/Qualifiers
     source          1..8815
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     629..648
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    677..701
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3918..3942)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3959..4000
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             4007..4048
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             4079..4120
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             4130..4147
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(4170..4187)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4176..4400
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4446..4874
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4533..4552)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4743..4764
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4883..4903)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4888..5217
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5068..5203
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5130..5149
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5284..6078
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5338..5357)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5948..5967
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6254..6375
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6291..6310)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6345..6364
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6424..6440)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6424..6440)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6437..6459)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6448..6464
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6472..6478,6479..6496,6497..6502))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6517..6538
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6655..6672)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6826..7414)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6906..6925)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7585..8376,8377..8445))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8208..8227
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8446..8550)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8625..8644)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctg
      661 gctagttaag ctatcaacaa gtttgtacaa aaaagttggc accatggcga aggcgacgtc
      721 cggtgccgcg gggctgcgtc tgctgttgct gctgctgctg ccgctgctag gcaaagtggc
      781 attgggcctc tacttctcga gggatgctta ctgggagaag ctgtatgtgg accaggcggc
      841 cggcacgccc ttgctgtacg tccatgccct gcgggacgcc cctgaggagg tgcccagctt
      901 ccgcctgggc cagcatctct acggcacgta ccgcacacgg ctgcatgaga acaactggat
      961 ctgcatccag gaggacaccg gcctcctcta ccttaaccgg agcctggacc atagctcctg
     1021 ggagaagctc agtgtccgca accgcggctt tcccctgctc accgtctacc tcaaggtctt
     1081 cctgtcaccc acatcccttc gtgagggcga gtgccagtgg ccaggctgtg cccgcgtata
     1141 cttctccttc ttcaacacct cctttccagc ctgcagctcc ctcaagcccc gggagctctg
     1201 cttcccagag acaaggccct ccttccgcat tcgggagaac cgacccccag gcaccttcca
     1261 ccagttccgc ctgctgcctg tgcagttctt gtgccccaac atcagcgtgg cctacaggct
     1321 cctggagggt gagggtctgc ccttccgctg cgccccggac agcctggagg tgagcacgcg
     1381 ctgggccctg gaccgcgagc agcgggagaa gtacgagctg gtggccgtgt gcaccgtgca
     1441 cgccggcgcg cgcgaggagg tggtgatggt gcccttcccg gtgaccgtgt acgacgagga
     1501 cgactcggcg cccaccttcc ccgcgggcgt cgacaccgcc agcgccgtgg tggagttcaa
     1561 gcggaaggag gacaccgtgg tggccacgct gcgtgtcttc gatgcagacg tggtacctgc
     1621 atcaggggag ctggtgaggc ggtacacaag cacgctgctc cccggggaca cctgggccca
     1681 gcagaccttc cgggtggaac actggcccaa cgagacctcg gtccaggcca acggcagctt
     1741 cgtgcgggcg accgtacatg actataggct ggttctcaac cggaacctct ccatctcgga
     1801 gaaccgcacc atgcagctgg cggtgctggt caatgactca gacttccagg gcccaggagc
     1861 gggcgtcctc ttgctccact tcaacgtgtc ggtgctgccg gtcagcctgc acctgcccag
     1921 tacctactcc ctctccgtga gcaggagggc tcgccgattt gcccagatcg ggaaagtctg
     1981 tgtggaaaac tgccaggcat tcagtggcat caacgtccag tacaagctgc attcctctgg
     2041 tgccaactgc agcacgctag gggtggtcac ctcagccgag gacacctcgg ggatcctgtt
     2101 tgtgaatgac accaaggccc tgcggcggcc caagtgtgcc gaacttcact acatggtggt
     2161 ggccaccgac cagcagacct ctaggcaggc ccaggcccag ctgcttgtaa cagtggaggg
     2221 gtcatatgtg gccgaggagg cgggctgccc cctgtcctgt gcagtcagca agagacggct
     2281 ggagtgtgag gagtgtggcg gcctgggctc cccaacaggc aggtgtgagt ggaggcaagg
     2341 agatggcaaa gggatcacca ggaacttctc cacctgctct cccagcacca agacctgccc
     2401 cgacggccac tgcgatgttg tggagaccca agacatcaac atttgccctc aggactgcct
     2461 ccggggcagc attgttgggg gacacgagcc tggggagccc cgggggatta aagctggcta
     2521 tggcacctgc aactgcttcc ctgaggagga gaagtgcttc tgcgagcccg aagacatcca
     2581 ggatccactg tgcgacgagc tgtgccgcac ggtgatcgca gccgctgtcc tcttctcctt
     2641 catcgtctcg gtgctgctgt ctgccttctg catccactgc taccacaagt ttgcccacaa
     2701 gccacccatc tcctcagctg agatgacctt ccggaggccc gcccaggcct tcccggtcag
     2761 ctactcctct tccagtgccc gccggccctc gctggactcc atggagaacc aggtctccgt
     2821 ggatgccttc aagatcctgg aggatccaaa gtgggaattc cctcggaaga acttggttct
     2881 tggaaaaact ctaggagaag gcgaatttgg aaaagtggtc aaggcaacgg ccttccatct
     2941 gaaaggcaga gcagggtaca ccacggtggc cgtgaggatg ctgaaagaga acgcctcccc
     3001 gagtgagctt cgagacctgc tgtcagagtt caacgtcctg aagcaggtca accacccaca
     3061 tgtcatcaaa ttgtatgggg cctgcagcca ggatggcccg ctcctcctca tcgtggagta
     3121 cgccaaatac ggctccctgc ggggcttcct ccgcgagagc cgcaaagtgg ggcctggcta
     3181 cctgggcagt ggaggcagcc gcaactccag ctccctggac cacccggatg agcgggccct
     3241 caccatgggc gacctcatct catttgcctg gcagatctca caggggatgc agtatctggc
     3301 cgagatgaag ctcgttcatc gggacttggc agccagaaac atcctggtag ctgaggggcg
     3361 gaagatgaag atttcggatt tcggcttgtc ccgagatgtt tatgaagagg attcgtacgt
     3421 gaagaggagc cagggtcgga ttccagttaa atggatggca attgaatccc tttttgatca
     3481 tatctacacc acgcaaagtg atgtatggtc ttttggtgtc ctgctgtggg agatcgtgac
     3541 cctaggggga aacccctatc ctgggattcc tcctgagcgg ctcttcaacc ttctgaagac
     3601 cggccaccgg atggagaggc cagacaactg cagcgaggag atgtactgcc tgatgctgca
     3661 atgctggaag caggagccgg acaaaaggcc ggtgtttgcg gacatcagca aagacctgga
     3721 gaagatgatg gttaagagga gagactactt ggaccttgcg gcgtccactc catctgactc
     3781 cctgatttat gacgacggcc tctcagagga ggagacaccg ctggtggact gtaataatgc
     3841 ccccctccct cgagccctcc cttccacatg gattgaaaac aaactctatg gtagaatttc
     3901 ccatgcattt actagattcc caactttctt gtacaaagtg gttgctctag aaggctctgg
     3961 taaaccgatt ccgaacccgc tgctgggtct ggattctacc ggttccggca aaccgatccc
     4021 gaacccgctg ctgggcctgg actctaccgg ttctggtcta gagggcccgc ggttcgaagg
     4081 taagcctatc cctaaccctc tcctcggtct cgattctacg cgtaccggtc atcatcacca
     4141 tcaccattga gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc
     4201 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct
     4261 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg
     4321 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg
     4381 ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta
     4441 tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     4501 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     4561 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     4621 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
     4681 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     4741 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
     4801 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     4861 atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc
     4921 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga
     4981 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     5041 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     5101 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc
     5161 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
     5221 ctcccgggag cttgtatatc cattttcgga tctgatcaag agacaggatg aggatcgttt
     5281 cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta
     5341 ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg
     5401 tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa
     5461 ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct
     5521 gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg
     5581 caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca
     5641 atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat
     5701 cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac
     5761 gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc
     5821 gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa
     5881 aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag
     5941 gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc
     6001 ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt
     6061 cttgacgagt tcttctgagc gggactctgg ggttcgcgaa atgaccgacc aagcgacgcc
     6121 caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg
     6181 aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt
     6241 cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     6301 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     6361 catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc
     6421 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     6481 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     6541 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     6601 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     6661 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     6721 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     6781 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     6841 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     6901 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     6961 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     7021 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     7081 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     7141 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     7201 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     7261 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     7321 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     7381 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     7441 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     7501 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     7561 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     7621 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     7681 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     7741 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     7801 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     7861 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     7921 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     7981 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     8041 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     8101 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     8161 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     8221 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     8281 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     8341 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     8401 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     8461 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     8521 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
     8581 cgacggatcg ggagatctcc cgatccccta tggtgcactc tcagtacaat ctgctctgat
     8641 gccgcatagt taagccagta tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc
     8701 gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg acaattgcat gaagaatctg
     8761 cttagggtta ggcgttttgc gctgcttcgc gatgtacggg ccagatatac gcgtt
//
