LOCUS       sequence-297105-       10995 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses a regulatable secretory cargo for Arabidopsis thaliana.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10995)
  AUTHORS   Casler JC, Zajac AL, Valbuena FM, Sparvoli D, Jeyifous O, Turkewitz 
            AP, Horne-Badovinac S, Green WN, Glick BS
  TITLE     ESCargo: a regulatable fluorescent secretory cargo for diverse model
            organisms.
  JOURNAL   Mol Biol Cell. 2020 Oct 28:mbcE20090591. doi: 
            10.1091/mbc.E20-09-0591.
  PUBMED    33112725
REFERENCE   2  (bases 1 to 10995)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell. 2020 Oct 28:mbcE20090591. doi: 10.1091/mbc.E20-09-0591."
COMMENT     Sequence Label: 
            pEarleyGate100-pSpoA-APVNTT-DsRed-Express2-FKBP(LV)(C22V)
FEATURES             Location/Qualifiers
     source          1..10995
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        615..960
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     903..923
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     terminator      2074..2781
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     primer_bind     complement(2806..2823)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2806..2822)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2815..2837)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    3025..3049
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             4350..4979
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             5408..6481
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      6547..6741
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(6977..6999)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    7085..7225
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     7140..7159
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7240..7257)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7411..7999)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7491..7510)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(8086..8880)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    9305..9329
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(9415..9667)
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     CDS             complement(9677..10228)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(10234..10614)
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
ORIGIN
        1 cagccccaaa agagatctcc tttgccccgg agattacaat ggacgatttc ctctatcttt
       61 acgatctagg aaggaagttc gaaggtgaag gtgacgacac tatgttcacc actgataatg
      121 agaaggttag cctcttcaat ttcagaaaga atgctgaccc acagatggtt agagaggcct
      181 acgcagcagg tctcatcaag acgatctacc cgagtaacaa tctccaggag atcaaatacc
      241 ttcccaagaa ggttaaagat gcagtcaaaa gattcaggac taattgcatc aagaacacag
      301 agaaagacat atttctcaag atcagaagta ctattccagt atggacgatt caaggcttgc
      361 ttcataaacc aaggcaagta atagagattg gagtctctaa aaaggtagtt cctactgaat
      421 ctaaggccat gcatggagtc taagattcaa atcgaggatc taacagaact cgccgtgaag
      481 actggcgaac agttcataca gagtctttta cgactcaatg acaagaagaa aatcttcgtc
      541 aacatggtgg agcacgacac tctggtctac tccaaaaatg tcaaagatac agtctcagaa
      601 gaccaaaggg ctattgagac ttttcaacaa aggataattt cgggaaacct cctcggattc
      661 cattgcccag ctatctgtca cttcatcgaa aggacagtag aaaaggaagg tggctcctac
      721 aaatgccatc attgcgataa aggaaaggct atcattcaag atctctctgc cgacagtggt
      781 cccaaagatg gacccccacc cacgaggagc atcgtggaaa aagaagacgt tccaaccacg
      841 tcttcaaagc aagtggattg atgtgacatc tccactgacg taagggatga cgcacaatcc
      901 cactatcctt cgcaagaccc ttcctctata taaggaagtt catttcattt ggagaggaca
      961 cgctcgacat gaaagccttc acactcgctc tcttcttagc tctttccctc tatctcctgc
     1021 ccaatccagc cgcgccggta aacacaactg acagcacaga aaacgttata aaacccttta
     1081 tgcgttttaa agtccatatg gaggggagcg tcaatggaca cgaatttgag attgagggtg
     1141 aaggagaggg caagccttac gagggaaccc aaactgcgaa gctacaggtg accaagggcg
     1201 gacccctccc atttgcttgg gacattctgt ctccgcaatt ccaatatggc agtaaagtgt
     1261 atgtcaaaca cccggccgac ataccggact acaagaagtt atcatttccg gaaggtttca
     1321 agtgggagag agtcatgaat tttgaagacg ggggggtagt aacagtgact caggacagca
     1381 gtcttcaaga cggtacgttc atctatcatg tcaaatttat cggagtcaac ttcccttctg
     1441 acggaccggt tatgcagaag aagactctgg gctgggaacc gagtactgag cgactgtacc
     1501 ctcgtgatgg agtactgaaa ggtgagatac ataaagcact aaagctcaag ggcggcggtc
     1561 attatctcgt ggaatttaag agtatttaca tggctaaaaa acccgtaaag ctacctggat
     1621 attattatgt tgattctaaa ctagatatta cgagccataa cgaagactat accgtcgtcg
     1681 agcagtatga gagagcagag gcgcgacatc atttattcca gggctcaagc agaggagttc
     1741 aggtcgaaac tattagcccc ggagatggcc gaacctttcc gaaaaggggc cagactgtag
     1801 ttgtacacta tacgggaatg ctcgaggacg gaaagaagtt agacagctcc cgagacagaa
     1861 acaaaccgtt caagttcatg ctgggtaagc aagaagtaat tcgaggctgg gaagaaggcg
     1921 tagcccaaat gagcgtagga caacgagcca aacttacgat ttcaccagat tacgcctatg
     1981 gggctactgg acatcccgga gtgatacctc ctcatgcaac gctcgttttt gatgttgagt
     2041 tattgaagtt agagtaaact agtccctaga gtcctgcttt aatgagatat gcgagacgcc
     2101 tatgatcgca tgatatttgc tttcaattct gttgtgcacg ttgtaaaaaa cctgagcatg
     2161 tgtagctcag atccttaccg ccggtttcgg ttcattctaa tgaatatatc acccgttact
     2221 atcgtatttt tatgaataat attctccgtt caatttactg attgtaccct actacttata
     2281 tgtacaatat taaaatgaaa acaatatatt gtgctgaata ggtttatagc gacatctatg
     2341 atagagcgcc acaataacaa acaattgcgt tttattatta caaatccaat tttaaaaaaa
     2401 gcggcagaac cggtcaaacc taaaagactg attacataaa tcttattcaa atttcaaaag
     2461 tgccccaggg gctagtatct acgacacacc gagcggcgaa ctaataacgc tcactgaagg
     2521 gaactccggt tccccgccgg cgcgcatggg tgagattcct tgaagttgag tattggccgt
     2581 ccgctctacc gaaagttacg ggcaccattc aacccggtcc agcacggcgg ccgggtaacc
     2641 gacttgctgc cccgagaatt atgcagcatt tttttggtgt atgtgggccc caaatgaagt
     2701 gcaggtcaaa ccttgacagt gacgacaaat cgttgggcgg gtccagggcg aattttgcga
     2761 caacatgtcg aggctcagca ggacctgcag gcatgcaagc ttggcactgg ccgtcgtttt
     2821 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc
     2881 ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt
     2941 gcgcagcctg aatggcgaat gctagagcag cttgagcttg gatcagattg tcgtttcccg
     3001 ccttcagttt aaactatcag tgtttgacag gatatattgg cgggtaaacc taagagaaaa
     3061 gagcgtttat tagaataatc ggatatttaa aagggcgtga aaaggtttat ccgttcgtcc
     3121 atttgtatgt gcatgccaac cacagggttc ccctcgggat caaagtactt tgatccaacc
     3181 cctccgctgc tatagtgcag tcggcttctg acgttcagtg cagccgtctt ctgaaaacga
     3241 catgtcgcac aagtcctaag ttacgcgaca ggctgccgcc ctgccctttt cctggcgttt
     3301 tcttgtcgcg tgttttagtc gcataaagta gaatacttgc gactagaacc ggagacatta
     3361 cgccatgaac aagagcgccg ccgctggcct gctgggctat gcccgcgtca gcaccgacga
     3421 ccaggacttg accaaccaac gggccgaact gcacgcggcc ggctgcacca agctgttttc
     3481 cgagaagatc accggcacca ggcgcgaccg cccggagctg gccaggatgc ttgaccacct
     3541 acgccctggc gacgttgtga cagtgaccag gctagaccgc ctggcccgca gcacccgcga
     3601 cctactggac attgccgagc gcatccagga ggccggcgcg ggcctgcgta gcctggcaga
     3661 gccgtgggcc gacaccacca cgccggccgg ccgcatggtg ttgaccgtgt tcgccggcat
     3721 tgccgagttc gagcgttccc taatcatcga ccgcacccgg agcgggcgcg aggccgccaa
     3781 ggcccgaggc gtgaagtttg gcccccgccc taccctcacc ccggcacaga tcgcgcacgc
     3841 ccgcgagctg atcgaccagg aaggccgcac cgtgaaagag gcggctgcac tgcttggcgt
     3901 gcatcgctcg accctgtacc gcgcacttga gcgcagcgag gaagtgacgc ccaccgaggc
     3961 caggcggcgc ggtgccttcc gtgaggacgc attgaccgag gccgacgccc tggcggccgc
     4021 cgagaatgaa cgccaagagg aacaagcatg aaaccgcacc aggacggcca ggacgaaccg
     4081 tttttcatta ccgaagagat cgaggcggag atgatcgcgg ccgggtacgt gttcgagccg
     4141 cccgcgcacg tctcaaccgt gcggctgcat gaaatcctgg ccggtttgtc tgatgccaag
     4201 ctggcggcct ggccggccag cttggccgct gaagaaaccg agcgccgccg tctaaaaagg
     4261 tgatgtgtat ttgagtaaaa cagcttgcgt catgcggtcg ctgcgtatat gatgcgatga
     4321 gtaaataaac aaatacgcaa ggggaacgca tgaaggttat cgctgtactt aaccagaaag
     4381 gcgggtcagg caagacgacc atcgcaaccc atctagcccg cgccctgcaa ctcgccgggg
     4441 ccgatgttct gttagtcgat tccgatcccc agggcagtgc ccgcgattgg gcggccgtgc
     4501 gggaagatca accgctaacc gttgtcggca tcgaccgccc gacgattgac cgcgacgtga
     4561 aggccatcgg ccggcgcgac ttcgtagtga tcgacggagc gccccaggcg gcggacttgg
     4621 ctgtgtccgc gatcaaggca gccgacttcg tgctgattcc ggtgcagcca agcccttacg
     4681 acatatgggc caccgccgac ctggtggagc tggttaagca gcgcattgag gtcacggatg
     4741 gaaggctaca agcggccttt gtcgtgtcgc gggcgatcaa aggcacgcgc atcggcggtg
     4801 aggttgccga ggcgctggcc gggtacgagc tgcccattct tgagtcccgt atcacgcagc
     4861 gcgtgagcta cccaggcact gccgccgccg gcacaaccgt tcttgaatca gaacccgagg
     4921 gcgacgctgc ccgcgaggtc caggcgctgg ccgctgaaat taaatcaaaa ctcatttgag
     4981 ttaatgaggt aaagagaaaa tgagcaaaag cacaaacacg ctaagtgccg gccgtccgag
     5041 cgcacgcagc agcaaggctg caacgttggc cagcctggca gacacgccag ccatgaagcg
     5101 ggtcaacttt cagttgccgg cggaggatca caccaagctg aagatgtacg cggtacgcca
     5161 aggcaagacc attaccgagc tgctatctga atacatcgcg cagctaccag agtaaatgag
     5221 caaatgaata aatgagtaga tgaattttag cggctaaagg aggcggcatg gaaaatcaag
     5281 aacaaccagg caccgacgcc gtggaatgcc ccatgtgtgg aggaacgggc ggttggccag
     5341 gcgtaagcgg ctgggttgcc tgccggccct gcaatggcac tggaaccccc aagcccgagg
     5401 aatcggcgtg agcggtcgca aaccatccgg cccggtacaa atcggcgcgg cgctgggtga
     5461 tgacctggtg gagaagttga aggccgcgca ggccgcccag cggcaacgca tcgaggcaga
     5521 agcacgcccc ggtgaatcgt ggcaagcggc cgctgatcga atccgcaaag aatcccggca
     5581 accgccggca gccggtgcgc cgtcgattag gaagccgccc aagggcgacg agcaaccaga
     5641 ttttttcgtt ccgatgctct atgacgtggg cacccgcgat agtcgcagca tcatggacgt
     5701 ggccgttttc cgtctgtcga agcgtgaccg acgagctggc gaggtgatcc gctacgagct
     5761 tccagacggg cacgtagagg tttccgcagg gccggccggc atggccagtg tgtgggatta
     5821 cgacctggta ctgatggcgg tttcccatct aaccgaatcc atgaaccgat accgggaagg
     5881 gaagggagac aagcccggcc gcgtgttccg tccacacgtt gcggacgtac tcaagttctg
     5941 ccggcgagcc gatggcggaa agcagaaaga cgacctggta gaaacctgca ttcggttaaa
     6001 caccacgcac gttgccatgc agcgtacgaa gaaggccaag aacggccgcc tggtgacggt
     6061 atccgagggt gaagccttga ttagccgcta caagatcgta aagagcgaaa ccgggcggcc
     6121 ggagtacatc gagatcgagc tagctgattg gatgtaccgc gagatcacag aaggcaagaa
     6181 cccggacgtg ctgacggttc accccgatta ctttttgatc gatcccggca tcggccgttt
     6241 tctctaccgc ctggcacgcc gcgccgcagg caaggcagaa gccagatggt tgttcaagac
     6301 gatctacgaa cgcagtggca gcgccggaga gttcaagaag ttctgtttca ccgtgcgcaa
     6361 gctgatcggg tcaaatgacc tgccggagta cgatttgaag gaggaggcgg ggcaggctgg
     6421 cccgatccta gtcatgcgct accgcaacct gatcgagggc gaagcatccg ccggttccta
     6481 atgtacggag cagatgctag ggcaaattgc cctagcaggg gaaaaaggtc gaaaaggtct
     6541 ctttcctgtg gatagcacgt acattgggaa cccaaagccg tacattggga accggaaccc
     6601 gtacattggg aacccaaagc cgtacattgg gaaccggtca cacatgtaag tgactgatat
     6661 aaaagagaaa aaaggcgatt tttccgccta aaactcttta aaacttatta aaactcttaa
     6721 aacccgcctg gcctgtgcat aactgtctgg ccagcgcaca gccgaagagc tgcaaaaagc
     6781 gcctaccctt cggtcgctgc gctccctacg ccccgccgct tcgcgtcggc ctatcgcggc
     6841 cgctggccgc tcaaaaatgg ctggcctacg gccaggcaat ctaccagggc gcggacaagc
     6901 cgcgccgtcg ccactcgacc gccggcgccc acatcaaggc accctgcctc gcgcgtttcg
     6961 gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt
     7021 aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc
     7081 ggggcgcagc catgacccag tcacgtagcg atagcggagt gtatactggc ttaactatgc
     7141 ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg
     7201 cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg
     7261 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     7321 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     7381 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     7441 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     7501 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     7561 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     7621 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     7681 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     7741 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     7801 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt
     7861 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     7921 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     7981 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     8041 gaacgaaaac tcacgttaag ggattttggt catgcattct aggtactaaa acaattcatc
     8101 cagtaaaata taatatttta ttttctccca atcaggcttg atccccagta agtcaaaaaa
     8161 tagctcgaca tactgttctt ccccgatatc ctccctgatc gaccggacgc agaaggcaat
     8221 gtcataccac ttgtccgccc tgccgcttct cccaagatca ataaagccac ttactttgcc
     8281 atctttcaca aagatgttgc tgtctcccag gtcgccgtgg gaaaagacaa gttcctcttc
     8341 gggcttttcc gtctttaaaa aatcatacag ctcgcgcgga tctttaaatg gagtgtcttc
     8401 ttcccagttt tcgcaatcca catcggccag atcgttattc agtaagtaat ccaattcggc
     8461 taagcggctg tctaagctat tcgtataggg acaatccgat atgtcgatgg agtgaaagag
     8521 cctgatgcac tccgcataca gctcgataat cttttcaggg ctttgttcat cttcatactc
     8581 ttccgagcaa aggacgccat cggcctcact catgagcaga ttgctccagc catcatgccg
     8641 ttcaaagtgc aggacctttg gaacaggcag ctttccttcc agccatagca tcatgtcctt
     8701 ttcccgttcc acatcatagg tggtcccttt ataccggctg tccgtcattt ttaaatatag
     8761 gttttcattt tctcccacca gcttatatac cttagcagga gacattcctt ccgtatcttt
     8821 tacgcagcgg tatttttcga tcagtttttt caattccggt gatattctca ttttagccat
     8881 ttattatttc cttcctcttt tctacagtat ttaaagatac cccaagaagc taattataac
     8941 aagacgaact ccaattcact gttccttgca ttctaaaacc ttaaatacca gaaaacagct
     9001 ttttcaaagt tgttttcaaa gttggcgtat aacatagtat cgacggagcc gattttgaaa
     9061 ccgcggtgat cacaggcagc aacgctctgt catcgttaca atcaacatgc taccctccgc
     9121 gagatcatcc gtgtttcaaa cccggcagct tagttgccgt tcttccgaat agcatcggta
     9181 acatgagcaa agtctgccgc cttacaacgg ctctcccgct gacgccgtcc cggactgatg
     9241 ggctgcctgt atcgagtggt gattttgtgc cgagctgccg gtcggggagc tgttggctgg
     9301 ctggtggcag gatatattgt ggtgtaaaca aattgacgct tagacaactt aataacacat
     9361 tgcggacgtt tttaatgtac tgaattaacg ccgaattaat tcgagctcgg atctgataat
     9421 ttatttgaaa attcataaga aaagcaaacg ttacatgaat tgatgaaaca atacaaagac
     9481 agataaagcc acgcacattt aggatattgg ccgagattac tgaatattga gtaagatcac
     9541 ggaatttctg acaggagcat gtcttcaatt cagcccaaat ggcagttgaa atactcaaac
     9601 cgccccatat gcaggagcgg atcattcatt gtttgtttgg ttgcctttgc caacatggga
     9661 gtccaagatt ctgcagtcaa atctcggtga cgggcaggac cggacggggc ggtaccggca
     9721 ggctgaagtc cagctgccag aaacccacgt catgccagtt cccgtgcttg aagccggccg
     9781 cccgcagcat gccgcggggg gcatatccga gcgcctcgtg catgcgcacg ctcgggtcgt
     9841 tgggcagccc gatgacagcg accacgctct tgaagccctg tgcctccagg gacttcagca
     9901 ggtgggtgta gagcgtggag cccagtcccg tccgctggtg gcggggggag acgtacacgg
     9961 tcgactcggc cgtccagtcg taggcgttgc gtgccttcca ggggcccgcg taggcgatgc
    10021 cggcgacctc gccgtccacc tcggcgacga gccagggata gcgctcccgc agacggacga
    10081 ggtcgtccgt ccactcctgc ggttcctgcg gctcggtacg gaagttgacc gtgcttgtct
    10141 cgatgtagtg gttgacgatg gtgcagaccg ccggcatgtc cgcctcggtg gcacggcgga
    10201 tgtcggccgg gcgtcgttct gggctcatcg attcgatttg gtgtatcgag attggttatg
    10261 aaattcagat gctagtgtaa tgtattggta atttgggaag atataatagg aagcaaggct
    10321 atttatccat ttctgaaaag gcgaaatggc gtcaccgcga gcgtcacgcg cattccgttc
    10381 ttgctgtaaa gcgttgtttg gtacactttt gactagcgag gcttggcgtg tcagcgtatc
    10441 tattcaaaag tcgttaatgg ctgcggatca agaaaaagtt ggaatagaaa cagaataccc
    10501 gcgaaattca ggcccggttg ccatgtccta cacgccgaaa taaacgacca aattagtaga
    10561 aaaataaaaa ctgactcgga tacttacgtc acgtcttgcg cactgatttg aaaaatctca
    10621 gaattccaat cccacaaaaa tctgagctta acagcacagt tgctcctctc agagcagaat
    10681 cgggtattca acaccctcat atcaactact acgttgtgta taacggtcca catgccggta
    10741 tatacgatga ctggggttgt acaaaggcgg caacaaacgg cgttcccgga gttgcacaca
    10801 agaaatttgc cactattaca gaggcaagag cagcagctga cgcgtacaca acaagtcagc
    10861 aaacagacag gttgaacttc atccccaaag gagaagctca actcaagccc aagagctttg
    10921 ctaaggccct aacaagccca ccaaagcaaa aagcccactg gctcacgcta ggaaccaaaa
    10981 ggcccagcag tgatc
//
