LOCUS       sequence-297112-        9509 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector genome to ectopically express human TMPRSS2 
            variant 1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9509)
  AUTHORS   Rebendenne A, Valadao ALC, Tauziet M, Maarifi G, Bonaventure B, 
            McKellar J, Planes R, Nisole S, Arnaud-Arnould M, Moncorge O, Goujon
            C
  TITLE     SARS-CoV-2 triggers an MDA-5-dependent interferon response which is 
            unable to control replication in lung epithelial cells.
  JOURNAL   J Virol. 2021 Jan 29. pii: JVI.02415-20. doi: 10.1128/JVI.02415-20.
  PUBMED    33514628
REFERENCE   2  (bases 1 to 9509)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2021 Jan 29. pii: JVI.02415-20. doi: 10.1128/JVI.02415-20."
COMMENT     Sequence Label: RRL.sin.cPPT.SFFV/TMPRSS2 (variant 1).IRES-neo...
FEATURES             Location/Qualifiers
     source          1..9509
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        26..130
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(131..199,200..991)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(349..368)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1162..1750
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1651..1670
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1904..1921
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2038..2059
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2074..2079,2080..2097,2098..2104)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2112..2128
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2117..2139
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2136..2152
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2136..2152
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2171..2191
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2173..2191
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2219..2445
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2446..2626
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2673..2798
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3291..3524
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3709..3753
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4020..4137
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4184..4591
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     misc_feature    6208..6775
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    6225..6777
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6369..6386)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6596..6615
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6789..7583
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6843..6862)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7453..7472
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    7592..8180
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7645..7665)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8063..8074)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8267..8500
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8572..8693
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8609..8628)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8663..8682
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8712..8847
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8867..8886)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8868..8886)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8896..8913)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8896..8912)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8905..8927)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9054..9482
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9141..9160)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9351..9372
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
       61 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
      121 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
      181 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
      241 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
      301 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
      361 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
      421 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
      481 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
      541 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
      601 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
      661 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
      721 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
      781 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
      841 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
      901 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
      961 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     1021 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     1081 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     1141 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     1201 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     1261 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     1321 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     1381 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     1441 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     1501 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     1561 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     1621 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     1681 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     1741 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     1801 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     1861 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     1921 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     1981 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
     2041 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
     2101 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
     2161 cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagcttaatg
     2221 tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc
     2281 ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt
     2341 gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattgccg
     2401 cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc tctctggtta
     2461 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     2521 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     2581 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca
     2641 gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg
     2701 aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta
     2761 gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta
     2821 gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac
     2881 atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa
     2941 catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag
     3001 aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag
     3061 agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga
     3121 ccaccgcaca gcaaggggcc gctgatcttc agacctggag gaggagatat gagggacaat
     3181 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
     3241 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
     3301 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
     3361 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
     3421 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
     3481 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
     3541 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
     3601 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
     3661 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
     3721 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
     3781 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
     3841 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
     3901 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga
     3961 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcggttaact
     4021 tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata
     4081 gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttatc
     4141 gataatgaaa gaccccacct gtaggtttgg caagctagct gcagtaacgc cattttgcaa
     4201 ggcatggaaa aataccaaac caagaataga gaagttcaga tcaagggcgg gtacatgaaa
     4261 atagctaacg ttgggccaaa caggatatct gcggtgagca gtttcggccc cggcccgggg
     4321 ccaagaacag atggtcaccg cagtttcggc cccggcccga ggccaagaac agatggtccc
     4381 cagatatggc ccaaccctca gcagtttctt aagacccatc agatgtttcc aggctccccc
     4441 aaggacctga aatgaccctg cgccttattt gaattaacca atcagcctgc ttctcgcttc
     4501 tgttcgcgcg cttctgcttc ccgagctcta taaaagagct cacaacccct cactcggcgc
     4561 gccagtcctc cgacagactg agtcgcccgg ggggcggccg catgccccct gccccgcccg
     4621 gagttgaaag cgggtgtgag gagcgcggcg cggcaggtca tattgaacat tccagatacc
     4681 tatcattact cgatgctgtt gataacagca agatggcttt gaactcaggg tcaccaccag
     4741 ctattggacc ttactatgaa aaccatggat accaaccgga aaacccctat cccgcacagc
     4801 ccactgtggt ccccactgtc tacgaggtgc atccggctca gtactacccg tcccccgtgc
     4861 cccagtacgc cccgagggtc ctgacgcagg cttccaaccc tgtcgtctgc acgcagccca
     4921 aatccccatc cgggacagtg tgcacctcaa agactaagaa agcactgtgc atcaccttga
     4981 ccctggggac cttcctcgtg ggagctgcgc tggccgctgg cctactctgg aagttcatgg
     5041 gcagcaagtg ctccaactct gggatagagt gcgactcctc aggtacctgc atcaacccct
     5101 ctaactggtg tgatggcgtg tcacactgcc ccggcgggga ggacgagaat cggtgtgttc
     5161 gcctctacgg accaaacttc atccttcagg tgtactcatc tcagaggaag tcctggcacc
     5221 ctgtgtgcca agacgactgg aacgagaact acgggcgggc ggcctgcagg gacatgggct
     5281 ataagaataa tttttactct agccaaggaa tagtggatga cagcggatcc accagcttta
     5341 tgaaactgaa cacaagtgcc ggcaatgtcg atatctataa aaaactgtac cacagtgatg
     5401 cctgttcttc aaaagcagtg gtttctttac gctgtatagc ctgcggggtc aacttgaact
     5461 caagccgcca gagcaggatt gtgggcggtg agagcgcgct cccgggggcc tggccctggc
     5521 aggtcagcct gcacgtccag aacgtccacg tgtgcggagg ctccatcatc acccccgagt
     5581 ggatcgtgac agccgcccac tgcgtggaaa aacctcttaa caatccatgg cattggacgg
     5641 catttgcggg gattttgaga caatctttca tgttctatgg agccggatac caagtggaaa
     5701 aagtgatttc tcatccaaat tatgactcca agaccaagaa caatgacatt gcgctgatga
     5761 agctgcagaa gcctctgact ttcaacgacc tagtgaaacc agtgtgtctg cccaacccag
     5821 gcatgatgct gcagccagaa cagctctgct ggatttccgg gtggggagcc accgaggaga
     5881 aagggaagac ctcagaagtg ctgaacgctg ccaaggtgct tctcattgag acacagagat
     5941 gcaacagcag atatgtctat gacaacctga tcacaccagc catgatctgt gccggcttcc
     6001 tgcaggggaa cgtcgattct tgccagggtg acagtggagg gcctctggtc acttcgaaga
     6061 acaatatctg gtggctgata ggggatacaa gctggggttc tggctgtgcc aaagcttaca
     6121 gaccaggagt gtacgggaat gtgatggtat tcacggactg gatttatcga caaatgaggg
     6181 cagacggcta actcgaggtt aacgaattcc gccccccccc cctaacgtta ctggccgaag
     6241 ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta ttttccacca tattgccgtc
     6301 ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg
     6361 tctttcccct ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc
     6421 tctggaagct tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc
     6481 cccacctggc gacaggtgcc tctgcggcca aaagccacgt gtataagata cacctgcaaa
     6541 ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt gtggaaagag tcaaatggct
     6601 ctcctcaagc gtattcaaca aggggctgaa ggatgcccag aaggtacccc attgtatggg
     6661 atctgatctg gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt taaaaaaacg
     6721 tctaggcccc ccgaaccacg gggacgtggt tttcctttga aaaacacgat gataatatgg
     6781 ccacaaccat gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga
     6841 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc
     6901 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga
     6961 atgaactgca ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg
     7021 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc
     7081 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg
     7141 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga
     7201 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc
     7261 tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca
     7321 tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg
     7381 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct
     7441 atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg
     7501 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc
     7561 gccttcttga cgagttcttc tgaatgtcga caatcaacct ctggattaca aaatttgtga
     7621 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
     7681 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
     7741 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
     7801 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
     7861 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
     7921 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
     7981 ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg
     8041 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
     8101 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc
     8161 cctttgggcc gcctccccgc ctggaattcg agctcggtac ctttaagacc aatgacttac
     8221 aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt
     8281 cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag
     8341 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
     8401 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
     8461 tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt
     8521 attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt
     8581 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     8641 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     8701 tggctctagc tatcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     8761 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     8821 agtagtgagg aggctttttt ggaggcctag ggacgtaccc aattcgccct atagtgagtc
     8881 gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
     8941 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
     9001 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc
     9061 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     9121 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     9181 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     9241 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
     9301 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     9361 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     9421 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     9481 ttttaacaaa atattaacgc ttacaattt
//
