LOCUS       sequence-297121-        8462 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8462)
  AUTHORS   Khavari PA, Peterson CL, Tamkun JW, Mendel DB, Crabtree GR
  TITLE     BRG1 contains a conserved domain of the SWI2/SNF2 family necessary 
            for normal mitotic growth and transcription.
  JOURNAL   Nature. 1993 Nov 11. 366(6451):170-4.
  PUBMED    8232556
REFERENCE   2  (bases 1 to 8462)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            1993 Nov 11. 366(6451):170-4."
COMMENT     Sequence Label: pBJ5 hBRG1
FEATURES             Location/Qualifiers
     source          1..8462
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(1..330,331..345,346..614)
                     /label=SR-alpha promoter
                     /note="hybrid promoter consisting of the SV40 early 
                     promoter plus part of the long terminal repeat of human 
                     T-lymphotrophic virus 1"
     rep_origin      181..316
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     LTR             346..614
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     intron          658..754
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     CDS             5794..5820
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    5867..6001
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5891..5910)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5945..5964
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(6144..6166)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    6252..6392
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     6307..6326
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6407..6424)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6578..7166)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6658..6677)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7337..8128,8129..8197))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7960..7979
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8198..8302)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8370..8388
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(join(8458..8462,1..16))
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
       61 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
      121 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
      181 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
      241 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
      301 ggcttttttg gaggcctagg cttttgcaaa aagctcctcg atcgaggggc tcgcatctct
      361 ccttcacgcg cccgccgccc tacctgaggc cgccatccac gccggttgag tcgcgttctg
      421 ccgcctcccg cctgtggtgc ctcctgaact gcgtccgccg tctaggtaag tttaaagctc
      481 aggtcgagac cgggcctttg tccggcgctc ccttggagcc tacctagact cagccggctc
      541 tccacgcttt gcctgaccct gcttgctcaa ctctacgtct ttgtttcgtt ttctgttctg
      601 cgccgttaca gatccaagct cctcgatcga ggaactgaaa aaccagaaag ttaactggta
      661 agtttagtct ttttgtcttt tatttcaggt cccggatccg gtggtggtgc aaatcaaaga
      721 actgctcctc agtggatgtt gcctttactt ctaggcctgt acggaagtgt tacttctgct
      781 ctaaaagctg ctctagccct cgacggcgcc ggcggctcct gcaggaggcc actgtctgca
      841 gctcccgtga agatgtccac tccagaccca cccctgggcg gaactcctcg gccaggtcct
      901 tccccgggcc ctggcccttc ccctggagcc atgctgggcc ctagcccggg tccctcgccg
      961 ggctccgccc acagcatgat ggggcccagc ccagggccgc cctcagcagg acaccccatc
     1021 cccacccagg ggcctggagg gtaccctcag gacaacatgc accagatgca caagcccatg
     1081 gagtccatgc atgagaaggg catgtcggac gacccgcgct acaaccagat gaaaggaatg
     1141 gggatgcggt cagggggcca tgctgggatg gggcccccgc ccagccccat ggaccagcac
     1201 tcccaaggtt acccctcgcc cctgggtggc tctgagcatg cctctagtcc agttccagcc
     1261 agtggcccgt cttcggggcc ccagatgtct tccgggccag gaggtgcccc gctggatggt
     1321 gctgaccccc aggccttggg gcagcagaac cggggcccaa ccccatttaa ccagaaccag
     1381 ctgcaccagc tcagagctca gatcatggcc tacaagatgc tggccagggg gcagcccctc
     1441 cccgaccacc tgcagatggc ggtgcagggc aagcggccga tgcccgggat gcagcagcag
     1501 atgccaacgc tacctccacc ctcggtgtcc gcaacaggac ccggccctgg ccctggccct
     1561 ggccccggcc cgggtcccgg cccggcacct ccaaattaca gcaggcctca tggtatggga
     1621 gggcccaaca tgcctccccc aggaccctcg ggcgtgcccc ccgggatgcc aggccagcct
     1681 cctggagggc ctcccaagcc ctggcctgaa ggacccatgg cgaatgctgc tgcccccacg
     1741 agcacccctc agaagctgat tcccccgcag ccaacgggcc gcccttcccc cgcgccccct
     1801 gccgtcccac ccgccgcctc gcccgtgatg ccaccgcaga cccagtcccc cgggcagccg
     1861 gcccagcccg cgcccatggt gccactgcac cagaagcaga gccgcatcac ccccatccag
     1921 aagccgcggg gcctcgaccc tgtggagatc ctgcaggagc gcgagtacag gctgcaggct
     1981 cgcatcgcac accgaattca ggaacttgaa aaccttcccg ggtccctggc cggggatttg
     2041 cgaaccaaag cgaccattga gctcaaggcc ctcaggctgc tgaacttcca gaggcagctg
     2101 cgccaggagg tggtggtgtg catgcggagg gacacagcgc tggagacagc cctcaatgct
     2161 aaggcctaca agcgcagcaa gcgccagtcc ctgcgcgagg cccgcatcac tgagaagctg
     2221 gagaagcagc agaagatcga gcaggagcgc aagcgccggc agaagcacca ggaatacctc
     2281 aatagcattc tccagcatgc caaggatttc aaggaatatc acagatccgt cacaggcaaa
     2341 atccagaagc tgaccaaggc agtggccacg taccatgcca acacggagcg ggagcagaag
     2401 aaagagaacg agcggatcga gaaggagcgc atgcggaggc tcatggctga agatgaggag
     2461 gggtaccgca agctcatcga ccagaagaag gacaagcgcc tggcctacct cttgcagcag
     2521 acagacgagt acgtggctaa cctcacggag ctggtgcggc agcacaaggc tgcccaggtc
     2581 gccaaggaga aaaagaagaa aaagaaaaag aagaaggcag aaaatgcaga aggacagacg
     2641 cctgccattg ggccggatgg cgagcctctg gacgagacca gccagatgag cgacctcccg
     2701 gtgaaggtga tccacgtgga gagtgggaag atcctcacag gcacagatgc ccccaaagcc
     2761 gggcagctgg aggcctggct cgagatgaac ccggggtatg aagtagctcc gaggtctgat
     2821 agtgaagaaa gtggctcaga agaagaggaa gaggaggagg aggaagagca gccgcaggca
     2881 gcacagcctc ccaccctgcc cgtggaggag aagaagaaga ttccagatcc agacagcgat
     2941 gacgtctctg aggtggacgc gcggcacatc attgagaatg ccaagcaaga tgtcgatgat
     3001 gaatatggcg tgtcccaggc ccttgcacgt ggcctgcagt cctactatgc cgtggcccat
     3061 gctgtcactg agagagtgga caagcagtca gcgcttatgg tcaatggtgt cctcaaacag
     3121 taccagatca aaggtttgga gtggctggtg tccctgtaca acaacaacct gaacggcatc
     3181 ctggccgacg agatgggcct ggggaagacc atccagacca tcgcgctcat cacgtacctc
     3241 atggagcaca aacgcatcaa tgggcccttc ctcatcatcg tgcctctctc aacgctgtcc
     3301 aactgggcgt acgagtttga caagtgggcc ccctccgtgg tgaaggtgtc ttacaaggga
     3361 tccccagcag caagacgggc ctttgtcccc cagctccgga gtgggaagtt caacgtcttg
     3421 ctgacgacgt acgagtacat catcaaagac aagcacatcc tcgccaagat ccgttggaag
     3481 tacatgattg tggacgaagg tcaccgcatg aagaaccacc actgcaagct gacgcaggtg
     3541 ctcaacacgc actatgtggc accccgccgc ctgctgctga cgggcacacc gctgcagaac
     3601 aagcttcccg agctctgggc gctgctcaac ttcctgctgc ccaccatctt caagagctgc
     3661 agcaccttcg agcagtggtt taacgcaccc tttgccatga ccggggaaaa ggtggacctg
     3721 aatgaggagg aaaccattct catcatccgg cgtctccaca aagtgctgcg gcccttcttg
     3781 ctccgacgac tcaagaagga agtcgaggcc cagttgcccg aaaaggtgga gtacgtcatc
     3841 aagtgcgaca tgtctgcgct gcagcgagtg ctctaccgcc acatgcaggc caagggcgtg
     3901 ctgctgactg atggctccga gaaggacaag aagggcaaag gcggcaccaa gaccctgatg
     3961 aacaccatca tgcagctgcg gaagatctgc aaccacccct acatgttcca gcacatcgag
     4021 gagtcctttt ccgagcactt ggggttcact ggcggcattg tccaagggct ggacctgtac
     4081 cgagcctcgg gtaaatttga gcttcttgat agaattcttc ccaaactccg agcaaccaac
     4141 cacaaagtgc tgctgttctg ccaaatgacc tccctcatga ccatcatgga agattacttt
     4201 gcgtatcgcg gctttaaata cctcaggctt gatggaacca cgaaggcgga ggaccggggc
     4261 atgctgctga aaaccttcaa cgagcccggc tctgagtact tcatcttcct gctcagcacc
     4321 cgggctgggg ggctcggcct gaacctccag tcggcagaca ctgtgatcat ttttgacagc
     4381 gactggaatc ctcaccagga cctgcaagcg caggaccgag cccaccgcat cgggcagcag
     4441 aacgaggtgc gtgtgctccg cctctgcacc gtcaacagcg tggaggagaa gatcctagct
     4501 gcagccaagt acaagctcaa cgtggaccag aaggtgatcc aggccggcat gttcgaccag
     4561 aagtcctcca gccatgagcg gcgcgccttc ctgcaggcca tcctggagca cgaggagcag
     4621 gatgagagca gacactgcag cacgggcagc ggcagtgcca gcttcgccca cactgcccct
     4681 ccgccagcgg gcgtcaaccc cgacttggag gagccacctc taaaggagga agacgaggtg
     4741 cccgacgacg agaccgtcaa ccagatgatc gcccggcacg aggaggagtt tgatctgttc
     4801 atgcgcatgg acctggaccg caggcgcgag gaggcccgca accccaagcg gaagccgcgc
     4861 ctcatggagg aggacgagct cccctcgtgg atcatcaagg acgacgcgga ggtggagcgg
     4921 ctgacctgtg aggaggagga ggagaagatg ttcggccgtg gctcccgcca ccgcaaggag
     4981 gtggactaca gcgactcact gacggagaag cagtggctca aggccatcga ggagggcacg
     5041 ctggaggaga tcgaagagga ggtccggcag aagaaatcat cacggaagcg caagcgagac
     5101 agcgacgccg gctcctccac cccgaccacc agcacccgca gccgcgacaa ggacgacgag
     5161 agcaagaagc agaagaagcg cgggcggccg cctgccgaga aactctcccc taacccaccc
     5221 aacctcacca agaagatgaa gaagattgtg gatgccgtga tcaagtacaa ggacagcagc
     5281 agtggacgtc agctcagcga ggtcttcatc cagctgccct cgcgaaagga gctgcccgag
     5341 tactacgagc tcatccgcaa gcccgtggac ttcaagaaga taaaggagcg cattcgcaac
     5401 cacaagtacc gcagcctcaa cgacctagag aaggacgtca tgctcctgtg ccagaacgca
     5461 cagaccttca acctggaggg ctccctgatc tatgaagact ccatcgtctt gcagtcggtc
     5521 ttcaccagcg tgcggcagaa aatcgagaag gaggatgaca gtgaaggcga ggagagtgag
     5581 gaggaggaag agggcgagga ggaaggctcc gaatccgaat ctcggtccgt caaagtgaag
     5641 atcaagcttg gccggaagga gaaggcacag gaccagctga agggcggccg gcggcggccg
     5701 agccgagggt cccgagccaa gccggtcgtg agtgacgatg acagtgagga ggaacaagag
     5761 gaggaccgct caggaagtgg cagcgaagaa gactacccct acgacgtacc cgactacgct
     5821 tgaaagcttt gcggccgcgg aggccgaatt cctgattgat tgaccggatc cagacatgat
     5881 aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat
     5941 ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt
     6001 taacaacaac aattgcattc attttatgtt tcaggttcag ggggaggtgt gggaggtttt
     6061 ttaaagcaag taaaacctct acaaatgtgg tatggctgat tatgatccgg ctgcctcgcg
     6121 cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct
     6181 tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc
     6241 gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta tactggctta
     6301 actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc
     6361 acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg ctcactgact
     6421 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     6481 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     6541 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     6601 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     6661 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     6721 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     6781 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     6841 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     6901 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     6961 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
     7021 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     7081 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     7141 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     7201 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     7261 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     7321 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     7381 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     7441 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     7501 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     7561 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     7621 ttaatagttt gcgcaacgtt gttgccattg ctgcaggcat cgtggtgtca cgctcgtcgt
     7681 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     7741 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     7801 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     7861 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     7921 tgcggcgacc gagttgctct tgcccggcgt caacacggga taataccgcg ccacatagca
     7981 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     8041 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     8101 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     8161 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     8221 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     8281 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa
     8341 ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtcttc
     8401 aagaattggt cgaccaattc tcatgtttga cagcttatca tcgataagct tggctgtgga
     8461 at
//
