LOCUS       sequence-297127-        9573 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid with 4xBstTyrT(CUA) cassette and E. coli TyrRS for amber 
            suppression; for transient or stable piggyBac-mediated integration.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9573)
  AUTHORS   Meineke B, Heimgartner J, Caridha R, Block MF, Kimler KJ, Pires MF, 
            Landreh M, Elsasser SJ
  TITLE     Dual stop codon suppression in mammalian cells with genomically 
            integrated genetic code expansion machinery.
  JOURNAL   Cell Rep Methods. 2023 Nov 20;3(11):100626. doi: 
            10.1016/j.crmeth.2023.100626. Epub 2023 Nov 6.
  PUBMED    37935196
REFERENCE   2  (bases 1 to 9573)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep 
            Methods."; date: "2023-11-20"; volume: "3(11):100626. doi"; pages: "
            10.1016/j.crmeth.2023.100626. Epub 2023 Nov 6"
COMMENT     Sequence Label: pAS_4xBstTyrT(CUA)_EcoTyrRS-FLAG
FEATURES             Location/Qualifiers
     source          1..9573
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(1..105)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(131..586)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(268..289)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     480..499
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     713..735
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     727..744
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     728..744
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   874..936
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        2793..3004
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             3017..3285
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     regulatory      3317..3326
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4595..4618
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    4646..5218
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4813..4830)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5040..5059
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5227..6021
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLAATGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5281..5300)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5891..5910
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(6081..6101)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     polyA_signal    6143..6264
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6180..6199)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6234..6253
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   7027..7061
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(7552..7568)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7552..7568)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7565..7587)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7576..7592
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7600..7606,7607..7624,7625..7630))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7645..7666
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7783..7800)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7954..8542)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8034..8053)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8713..9504,9505..9573))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9336..9355
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
       61 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
      121 agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
      181 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
      241 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
      301 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
      361 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
      421 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
      481 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
      541 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc
      601 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
      661 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
      721 cgacgttgta aaacgacggc cagtgagcgc gcctcgttca ttcacgtttt tgaacccgtg
      781 gaggacgggc agactcgcgg tgcaaatgtg ttttacagcg tgatggagca gatgaagatg
      841 ctcgacacgc tgcagaacac gcagctagat taaccctaga aagataatca tattgtgacg
      901 tacgttaaag ataatcatgt gtaaaattga cgcatgtgtt ttatcggtct gtatatcgag
      961 gtttatttat taatttgaat agatattaag ttttattata tttacactta catactaata
     1021 ataaattcaa caaacaattt atttatgttt atttatttat taaaaaaaac aaaaactcaa
     1081 aatttcttct ataaagtaac aaaactttta tgagggacag ccccccccca aagcccccag
     1141 ggatgtaatt acgtccctcc cccgctaggg ggcagcagcg agccgcccgg ggctccgctc
     1201 cggtccggcg ctccccccgc atccccgagc cggcagcgtg cggggacagc ccgggcacgg
     1261 ggaaggtggc acgggatcgc tttcctctga acgcttctcg ctgctctttg agcctgcaga
     1321 cacctggggg gatacgggga aaaggcctcc acggccaact agtaaaaacc gcacttgtct
     1381 ggagggggac ggattcgaac cgccgaaccc aaagggagcg gatttagagt ccgccgcgtt
     1441 tagccacttc gctacccctc cgaggtaccc aggcggcgca caagctatat aaacctgaag
     1501 gaaatctcaa ctttacactt aggtcaagtt acttatcgta ctagagcttc agcaggaaat
     1561 ttaactaaaa tctaatttaa ccagcatagc aaatatcatt tattcccaaa atgctaaagt
     1621 ttgagataaa cggacttgat ttccggctgt tttgacacta tccagaatgc cttgcagatg
     1681 ggtggggcat gctaaatact gcaggaaaaa ccgcacttgt cyggaggggg acggattcga
     1741 accgccgaac ccaaagggag cggatttaga gtccgccgcg tttagccact tcgctacccc
     1801 tccgaggtac ccaggcggcg cacaagctat ataaacctga aggaaatctc aactttacac
     1861 ttaggtcaag ttacttatcg tactagagct tcagcaggaa atttaactaa aatctaattt
     1921 aaccagcata gcaaatatca tttattccca aaatgctaaa gtttgagata aacggacttg
     1981 atttccggct gttttgacac tatccagaat gccttgcaga tgggtggggc atgctaaata
     2041 ctgcagcatg aaaaaccgca cttgtctgga gggggacgga ttcgaaccgc cgaacccaaa
     2101 gggagcggat ttagagtccg ccgcgtttag ccacttcgct acccctccga ggtacccagg
     2161 cggcgcacaa gctatataaa cctgaaggaa atctcaactt tacacttagg tcaagttact
     2221 tatcgtacta gagcttcagc aggaaattta actaaaatct aatttaacca gcatagcaaa
     2281 tatcatttat tcccaaaatg ctaaagtttg agataaacgg acttgatttc cggctgtttt
     2341 gacactatcc agaatgcctt gcagatgggt ggggcatgct aaatactgca gcgataaaaa
     2401 ccgcacttgt ctggaggggg acggattcga accgccgaac ccaaagggag cggatttaga
     2461 gtccgccgcg tttagccact tcgctacccc tccgaggtac ccaggcggcg cacaagctat
     2521 ataaacctga aggaaatctc aactttacac ttaggtcaag ttacttatcg tactagagct
     2581 tcagcaggaa atttaactaa aatctaattt aaccagcata gcaaatatca tttattccca
     2641 aaatgctaaa gtttgagata aacggacttg atttccggct gttttgacac tatccagaat
     2701 gccttgcaga tgggtggggc atgctaaata ctgcagctcc aaggccacta gtgctctaga
     2761 aaggatctgc gatcgctccg gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc
     2821 cgagaagttg gggggagggg tcggcaattg aacgggtgcc tagagaaggt ggcgcggggt
     2881 aaactgggaa agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc
     2941 gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac
     3001 acagctgaag cttcgagggg ctcgcatctc tccttcacgc gcccgccgcc ctacctgagg
     3061 ccgccatcca cgccggttga gtcgcgttct gccgcctccc gcctgtggtg cctcctgaac
     3121 tgcgtccgcc gtctaggtaa gtttaaagct caggtcgaga ccgggccttt gtccggcgct
     3181 cccttggagc ctacctagac tcagccggct ctccacgctt tgcctgaccc tgcttgctca
     3241 actctacgtc tttgtttcgt tttctgttct gcgccgttac agatccaagc tgtgaccggc
     3301 gcctactcta gagctagcca ccatggcaag cagtaacttg attaaacaat tgcaagagcg
     3361 ggggctggta gcccaggtga cggacgagga agcgttagca gagcgactgg cgcaaggccc
     3421 gatcgcgctc ctgtgcggct tcgatcctac cgctgacagc ttgcatttgg ggcatcttgt
     3481 tccattgtta tgcctgaaac gcttccagca ggcgggccac aagccggttg cgctggtagg
     3541 cggcgcgacg ggtctgattg gcgacccgag cttcaaagct gccgagcgta agctgaacac
     3601 cgaagaaact gttcaggagt gggtggacaa aatccgtaag caggttgccc cgttcctcga
     3661 tttcgactgt ggagaaaact ctgctatcgc ggcgaacaac tatgactggt tcggcaatat
     3721 gaatgtgctg accttcctgc gcgatattgg caaacacttc tccgttaacc agatgatcaa
     3781 caaagaagcg gttaagcagc gtctcaaccg tgaagatcag gggatttcgt tcactgagtt
     3841 ttcctacaac ctgttgcagg gttatagcat ggcctgtgcg aacaaacagt acggtgtggt
     3901 gctgcaaatt ggtggttctg accagtgggg taacatcact tctggtatcg acctgacccg
     3961 tcgtctgcat cagaatcagg tgtttggcct gaccgttccg ctgatcacta aagcagatgg
     4021 caccaaattt ggtaaaactg aaggcggcgc agtctggttg gacccgaaga aaaccagccc
     4081 gtacaaattc taccagttct ggatcaacac tgcggatgcc gacgtttacc gcttcctgaa
     4141 gttcttcacc tttatgagca ttgaagagat caacgccctg gaagaagaag ataaaaacag
     4201 cggtaaagca ccgcgcgccc agtatgtact ggcggagcag gtgactcgtc tggttcacgg
     4261 tgaagaaggt ttacaggcgg caaaacgtat taccgaatgc ctgttcagcg gttctttgag
     4321 tgcgctgagt gaagcggact tcgaacagct ggcgcaggac ggcgtaccga tggttgagat
     4381 ggaaaagggc gcagacctga tgcaggcact ggtcgattct gaactgcaac cttcccgtgg
     4441 tcaggcacgt aaaactatcg cctccaatgc catcaccatt aacggtgaaa aacagtccga
     4501 tcctgaatac ttctttaaag aagaagatcg tctgtttggt cgttttacct tactgcgtcg
     4561 cggtaaaaag aattactgtc tgatttgctg gaaagactac aaagacgatg acgacaagtg
     4621 atgaatcgat ggatccgcgg ccgcgcccct ctccctcccc cccccctaac gttactggcc
     4681 gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat gttattttcc accatattgc
     4741 cgtcttttgg caatgtgagg gcccggaaac ctggccctgt cttcttgacg agcattccta
     4801 ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag
     4861 ttcctctgga agcttcttga agacaaacaa cgtctgtagc gaccctttgc aggcagcgga
     4921 accccccacc tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa gatacacctg
     4981 caaaggcggc acaaccccag tgccacgttg tgagttggat agttgtggaa agagtcaaat
     5041 ggctctcctc aagcgtattc aacaaggggc tgaaggatgc ccagaaggta ccccattgta
     5101 tgggatctga tctggggcct cggtgcacat gctttacatg tgtttagtcg aggttaaaaa
     5161 aacgtctagg ccccccgaac cacggggacg tggttttcct ttgaaaaaca cgataatacc
     5221 tccggaatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg
     5281 ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg
     5341 ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat
     5401 gaactgcagg acgaggcagc gcggctatcg tggctggccg cgacgggcgt tccttgcgca
     5461 gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg
     5521 gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat
     5581 gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa
     5641 catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg
     5701 gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg
     5761 cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg
     5821 gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat
     5881 caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac
     5941 cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc
     6001 cttcttgacg agttcttctg agtcgacaat caacctctgg attacaaaat ttgtgaaaga
     6061 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     6121 cctttgtatc atgcgttaac taaacttgtt tattgcagct tataatggtt acaaataaag
     6181 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     6241 gtccaaactc atcaatgtat cttatcatgt ctggaattga ctcaaatgat gtcaattagt
     6301 ctatcagaag ctcatctggt ctcccttccg ggggacaaga catccctgtt taatatttaa
     6361 acagcagtgt tcccaaactg ggttcttata tcccttgctc tggtcaacca ggttgcaggg
     6421 tttcctgtcc tcacaggaac gaagtcccta aagaaacagt ggcagccagg tttagccccg
     6481 gaattgactg gattcctttt ttagggccca ttggtatggc tttttccccg tatcccccca
     6541 ggtgtctgca ggctcaaaga gcagcgagaa gcgttcagag gaaagcgatc ccgtgccacc
     6601 ttccccgtgc ccgggctgtc cccgcacgct gccggctcgg ggatgcgggg ggagcgccgg
     6661 accggagcgg agccccgggc ggctcgctgc tgccccctag cgggggaggg acgtaattac
     6721 atccctgggg gctttggggg ggggctgtcc ctgatatcta taacaagaaa atatatatat
     6781 aataagttat cacgtaagta gaacatgaaa taacaatata attatcgtat gagttaaatc
     6841 ttaaaagtca cgtaaaagat aatcatgcgt cattttgact cacgcggtcg ttatagttca
     6901 aaatcagtga cacttaccgc attgacaagc acgcctcacg ggagctccaa gcggcgactg
     6961 agatgtccta aatgcacagc gacggattcg cgctatttag aaagagagag caatatttca
     7021 agaatgcatg cgtcaatttt acgcagacta tctttctagg gttaatctag ctgcatcagg
     7081 atcatatcgt cgggtctttt ttccggctca gtcatcgccc aagctggcgc tatctgggca
     7141 tcggggagga agaagcccgt gccttttccc gcgaggttga agcggcatgg aaagagtttg
     7201 ccgaggatga ctgctgctgc attgacgttg agcgaaaacg cacgtttacc atgatgattc
     7261 gggaaggtgt ggccatgcac gcctttaacg gtgaactgtt cgttcaggcc acctgggata
     7321 ccagttcgtc gcggcttttc cggacacagt tccggatggt cagcccgaag cgcatcagca
     7381 acccgaacaa taccggcgac agccggaact gccgtgccgg tgtgcagatt aatgacagcg
     7441 gtgcggcgct gggatattac gtcagcgagg acgggtatcc tggctggatg ccgcagaaat
     7501 ggacatggat accccgtgag ttacccggcg ggcgcgcttg gcgtaatcat ggtcatagct
     7561 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
     7621 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
     7681 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     7741 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     7801 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     7861 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     7921 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     7981 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     8041 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     8101 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     8161 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     8221 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     8281 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     8341 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     8401 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     8461 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     8521 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     8581 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     8641 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     8701 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     8761 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     8821 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     8881 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     8941 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     9001 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     9061 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     9121 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     9181 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     9241 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     9301 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     9361 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     9421 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     9481 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     9541 aataagggcg acacggaaat gttgaatact cat
//
