LOCUS       sequence-297145-       11927 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of FLAG-tagged TAPBPR with tapasin CT 
            delta24-36.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11927)
  AUTHORS   McShan AC, Devlin CA, Morozov GI, Overall SA, Moschidi D, Akella N, 
            Procko E, Sgourakis NG
  TITLE     TAPBPR promotes antigen loading on MHC-I molecules using a peptide 
            trap.
  JOURNAL   Nat Commun. 2021 May 26;12(1):3174. doi: 10.1038/s41467-021-23225-6.
  PUBMED    34039964
REFERENCE   2  (bases 1 to 11927)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-05-26"; volume: "12(1):3174. doi"; pages: " 
            10.1038/s41467-021-23225-6"
COMMENT     Sequence Label: pCEP4-FLAG-TAPBPR-tapasinCT delta24-36
FEATURES             Location/Qualifiers
     source          1..11927
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      7..16
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             92..115
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     polyA_signal    1560..1694
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1584..1603)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1638..1657
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(1836..1858)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      2444..4233
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     CDS             complement(4539..6464)
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     primer_bind     complement(6899..6917)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6985..7089
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7090..7158,7159..7950)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7308..7327)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8121..8709
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8610..8629
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        9326..9471
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             9511..10536
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             9511..10536
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     primer_bind     complement(10557..10576)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    10601..10649
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        11302..11681
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        11682..11885
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     11836..11856
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     11882..11906
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 gctagcgcca ccatgggccg tcatggcgcc ccgaaccctc gtcctgctac tctcgggggc
       61 cctggccctg acccagacct gggcgggctc cgactacaag gacgatgatg acaagggagg
      121 atccaagccc cacccagcag aggggcagtg gcgggcagtg gacgtggtcc tagactgttt
      181 cctggtgaag gacgcaaggg cctcccttgt gctgaagcag gtgccagtgc tggacgatgg
      241 ctccctggag gacttcaccg atttccaagg gggcacactg gcccaagatg acccacctat
      301 tatctttgag gcctcagtgg acctggtcca gattccccag gccgaggcct tgctccatgc
      361 tgactgcagt gggaaggagg tgacctgtga gatctcccgc tactttctcc agatgacaga
      421 gaccactgtt aagacagcag cttggttcat ggccaacgtg caggtctctg gagggggacc
      481 tagcatctcc ttggtgatga agactcccag ggtcgccaag aatgaggtgc tctggcaccc
      541 aacgctgaac ttgccactga gcccccaggg gactgtgcga actgcagtgg agttccaggt
      601 gatgacacag acccaatccc tgagcttcct gctggggtcc tcagcctcct tggactgtgg
      661 cttctccatg gcaccgggct tggacctcat cagtgtggag tggcgactgc agcacaaggg
      721 caggggtcag ttggtgtaca gctggaccgc agggcagggg caggctgtgc ggaagggcgc
      781 taccctggag cctgcacaac tgggcatggc cagggatgcc tccctcaccc tgcccggcct
      841 cactatacag gacgagggga cctacatttg ccagatcacc acctctctgt accgagctca
      901 gcagatcatc cagctcaaca tccaagcttc ccctaaagta cgactgagct tggcaaacga
      961 agctctgctg cccaccctca tctgcgacat tgctggctat taccctctgg atgtggtggt
     1021 gacgtggacc cgagaggagc tgggtggatc cccagcccaa gtctctggtg cctccttctc
     1081 cagcctcagg caaagcgtgg caggcaccta cagcatctcc tcctctctca ccgcagaacc
     1141 tggctctgca ggtgccactt acacctgcca ggtcacacac atctctctgg aggagcccct
     1201 tggggccagc acccaggttg tcccaccaga gcggagatca gggccctccc ttgaggacag
     1261 cgtaggcctt ttcctgtctg cctttcttct gcttgggctc ttcaaggcac tgggctgggc
     1321 tgctgtctac ctgtccacct gcaaggattc aaagaaggta cagtgctcca cctctctgta
     1381 tctttccctt gtcactttat ctcctcatcc tatctcaaaa cccatggagg gaggctgctg
     1441 gtgtggtagg cagaacctag gcttggaatt cacactgatc tgggttaaaa cctggcacta
     1501 tatcctaact gtaggactct ttgagcatgc tacttaatga ctcgaggccg gcaaggccgg
     1561 atccagacat gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa
     1621 aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct
     1681 gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg
     1741 tggggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtatggctg attatgatcc
     1801 ggctgcctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag
     1861 acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca
     1921 gcgggtgttg gcgggtgtcg gggcgcagcc atgaccggtc gactctagag ccccgccgcc
     1981 ggacgaacta aacctgacta cggcatctct gccccttctt cgctggtacg aggagcgctt
     2041 ttgttttgta ttcggggcag tgcatgtaat cccttcagtt ggttggtaca acttgccaac
     2101 tgggccctgt tccacatgtg acacgggggg ggaccaaaca caaaggggtt ctctgactgt
     2161 agttgacatc cttataaatg gatgtgcaca tttgccaaca ctgagtggct ttcatcctgg
     2221 agcagacttt gcatgctgtg gactgcaaca caacattgcc tttatgtgta actcttggct
     2281 gaagctctta caccaatgct gggggacatg tacctcccag gggcccagga agactacggg
     2341 aggctacacc aacgtcaatc agaggggcct gtgtagctac cgataagcgg accctcaaga
     2401 gggcattagc aatagtgttt ataaggcccc cttgttaacc ctaaacgggt agcatatgct
     2461 tcccgggtag tagtatatac tatccagact aaccctaatt caatagcata tgttacccaa
     2521 cgggaagcat atgctatcga attagggtta gtaaaagggt cctaaggaac agcgatatct
     2581 cccaccccat gagctgtcac ggttttattt acatggggtc aggattccac gagggtagtg
     2641 aaccatttta gtcacaaggg cagtggctga agatcaagga gcgggcagtg aactctcctg
     2701 aatcttcgcc tgcttcttca ttctccttcg tttagctaat agaataactg ctgagttgtg
     2761 aacagtaagg tgtatgtgag gtgctcgaaa acaaggtttc aggtgacgcc cccagaataa
     2821 aatttggacg gggggttcag tggtggcatt gtgctatgac accaatataa ccctcacaaa
     2881 ccccttgggc aataaatact agtgtaggaa tgaaacattc tgaatatctt taacaataga
     2941 aatccatggg gtggggacaa gccgtaaaga ctggatgtcc atctcacacg aatttatggc
     3001 tatgggcaac acataatcct agtgcaatat gatactgggg ttattaagat gtgtcccagg
     3061 cagggaccaa gacaggtgaa ccatgttgtt acactctatt tgtaacaagg ggaaagagag
     3121 tggacgccga cagcagcgga ctccactggt tgtctctaac acccccgaaa attaaacggg
     3181 gctccacgcc aatggggccc ataaacaaag acaagtggcc actctttttt ttgaaattgt
     3241 ggagtggggg cacgcgtcag cccccacacg ccgccctgcg gttttggact gtaaaataag
     3301 ggtgtaataa cttggctgat tgtaaccccg ctaaccactg cggtcaaacc acttgcccac
     3361 aaaaccacta atggcacccc ggggaatacc tgcataagta ggtgggcggg ccaagatagg
     3421 ggcgcgattg ctgcgatctg gaggacaaat tacacacact tgcgcctgag cgccaagcac
     3481 agggttgttg gtcctcatat tcacgaggtc gctgagagca cggtgggcta atgttgccat
     3541 gggtagcata tactacccaa atatctggat agcatatgct atcctaatct atatctgggt
     3601 agcataggct atcctaatct atatctgggt agcatatgct atcctaatct atatctgggt
     3661 agtatatgct atcctaattt atatctgggt agcataggct atcctaatct atatctgggt
     3721 agcatatgct atcctaatct atatctgggt agtatatgct atcctaatct gtatccgggt
     3781 agcatatgct atcctaatag agattagggt agtatatgct atcctaattt atatctgggt
     3841 agcatatact acccaaatat ctggatagca tatgctatcc taatctatat ctgggtagca
     3901 tatgctatcc taatctatat ctgggtagca taggctatcc taatctatat ctgggtagca
     3961 tatgctatcc taatctatat ctgggtagta tatgctatcc taatttatat ctgggtagca
     4021 taggctatcc taatctatat ctgggtagca tatgctatcc taatctatat ctgggtagta
     4081 tatgctatcc taatctgtat ccgggtagca tatgctatcc tcatgcatat acagtcagca
     4141 tatgataccc agtagtagag tgggagtgct atcctttgca tatgccgcca cctcccaagg
     4201 gggcgtgaat tttcgctgct tgtccttttc ctgcatgctg gttgctccca ttcttaggtg
     4261 aatttaagga ggccaggcta aagccgtcgc atgtctgatt gctcaccagg taaatgtcgc
     4321 taatgttttc caacgcgaga aggtgttgag cgcggagctg agtgacgtga caacatgggt
     4381 atgcccaatt gccccatgtt gggaggacga aaatggtgac aagacagatg gccagaaata
     4441 caccaacagc acgcatgatg tctactgggg atttattctt tagtgcgggg gaatacacgg
     4501 cttttaatac gattgagggc gtctcctaac aagttacatc actcctgccc ttcctcaccc
     4561 tcatctccat cacctccttc atctccgtca tctccgtcat caccctccgc ggcagcccct
     4621 tccaccatag gtggaaacca gggaggcaaa tctactccat cgtcaaagct gcacacagtc
     4681 accctgatat tgcaggtagg agcgggcttt gtcataacaa ggtccttaat cgcatccttc
     4741 aaaacctcag caaatatatg agtttgtaaa aagaccatga aataacagac aatggactcc
     4801 cttagcgggc caggttgtgg gccgggtcca ggggccattc caaaggggag acgactcaat
     4861 ggtgtaagac gacattgtgg aatagcaagg gcagttcctc gccttaggtt gtaaagggag
     4921 gtcttactac ctccatatac gaacacaccg gcgacccaag ttccttcgtc ggtagtcctt
     4981 tctacgtgac tcctagccag gagagctctt aaaccttctg caatgttctc aaatttcggg
     5041 ttggaacctc cttgaccacg atgctttcca aaccaccctc cttttttgcg cctgcctcca
     5101 tcaccctgac cccggggtcc agtgcttggg ccttctcctg ggtcatctgc ggggccctgc
     5161 tctatcgctc ccgggggcac gtcaggctca ccatctgggc caccttcttg gtggtattca
     5221 aaataatcgg cttcccctac agggtggaaa aatggccttc tacctggagg gggcctgcgc
     5281 ggtggagacc cggatgatga tgactgacta ctgggactcc tgggcctctt ttctccacgt
     5341 ccacgacctc tccccctggc tctttcacga cttccccccc tggctctttc acgtcctcta
     5401 ccccggcggc ctccactacc tcctcgaccc cggcctccac tacctcctcg accccggcct
     5461 ccactgcctc ctcgaccccg gcctccacct cctgctcctg cccctcctgc tcctgcccct
     5521 cctcctgctc ctgcccctcc tgcccctcct gctcctgccc ctcctgcccc tcctgctcct
     5581 gcccctcctg cccctcctgc tcctgcccct cctgcccctc ctcctgctcc tgcccctcct
     5641 gcccctcctc ctgctcctgc ccctcctgcc cctcctgctc ctgcccctcc tgcccctcct
     5701 gctcctgccc ctcctgcccc tcctgctcct gcccctcctg ctcctgcccc tcctgctcct
     5761 gcccctcctg ctcctgcccc tcctgcccct cctgcccctc ctcctgctcc tgcccctcct
     5821 gctcctgccc ctcctgcccc tcctgcccct cctgctcctg cccctcctcc tgctcctgcc
     5881 cctcctgccc ctcctgcccc tcctcctgct cctgcccctc ctgcccctcc tcctgctcct
     5941 gcccctcctc ctgctcctgc ccctcctgcc cctcctgccc ctcctcctgc tcctgcccct
     6001 cctgcccctc ctcctgctcc tgcccctcct cctgctcctg cccctcctgc ccctcctgcc
     6061 cctcctcctg ctcctgcccc tcctcctgct cctgcccctc ctgcccctcc tgcccctcct
     6121 gcccctcctc ctgctcctgc ccctcctcct gctcctgccc ctcctgctcc tgcccctccc
     6181 gctcctgctc ctgctcctgt tccaccgtgg gtccctttgc agccaatgca acttggacgt
     6241 ttttggggtc tccggacacc atctctatgt cttggccctg atcctgagcc gcccggggct
     6301 cctggtcttc cgcctcctcg tcctcgtcct cttccccgtc ctcgtccatg gttatcaccc
     6361 cctcttcttt gaggtccact gccgccggag ccttctggtc cagatgtgtc tcccttctct
     6421 cctaggccat ttccaggtcc tgtacctggc ccctcgtcag acatgattca cactaaaaga
     6481 gatcaataga catctttatt agacgacgct cagtgaatac agggagtgca gactcctgcc
     6541 ccctccaaca gcccccccac cctcatcccc ttcatggtcg ctgtcagaca gatccaggtc
     6601 tgaaaattcc ccatcctccg aaccatcctc gtcctcatca ccaattactc gcagcccgga
     6661 aaactcccgc tgaacatcct caagatttgc gtcctgagcc tcaagccagg cctcaaattc
     6721 ctcgtccccc tttttgctgg acggtaggga tggggattct cgggacccct cctcttcctc
     6781 ttcaaggtca ccagacagag atgctactgg ggcaacggaa gaaaagctgg gtgcggcctg
     6841 tgaggatcag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc
     6901 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca
     6961 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     7021 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     7081 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     7141 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     7201 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     7261 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     7321 gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     7381 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     7441 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     7501 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     7561 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     7621 accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     7681 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     7741 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     7801 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     7861 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     7921 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     7981 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     8041 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     8101 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     8161 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     8221 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag
     8281 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     8341 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     8401 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     8461 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     8521 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     8581 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     8641 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     8701 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctgcgccgcg
     8761 tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat
     8821 tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga
     8881 ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc
     8941 ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct
     9001 cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt
     9061 aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag
     9121 catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa
     9181 ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat
     9241 gccctgcttc atccccgtgg cccgttgctc gcgtttgctg gcggtgtccc cggaagaaat
     9301 atatttgcat gtctttagtt ctatgatgac acaaaccccg cccagcgtct tgtcattggc
     9361 gaattcgaac acgcagatgc agtcggggcg gcgcggtccc aggtccactt cgcatattaa
     9421 ggtgacgcgt gtggcctcga acaccgagcg accctgcagc gacccgctta acagcgtcaa
     9481 cagcgtgccg cagatcccgg gcaatgagat atgaaaaagc ctgaactcac cgcgacgtct
     9541 gtcgagaagt ttctgatcga aaagttcgac agcgtctccg acctgatgca gctctcggag
     9601 ggcgaagaat ctcgtgcttt cagcttcgat gtaggagggc gtggatatgt cctgcgggta
     9661 aatagctgcg ccgatggttt ctacaaagat cgttatgttt atcggcactt tgcatcggcc
     9721 gcgctcccga ttccggaagt gcttgacatt ggggaattca gcgagagcct gacctattgc
     9781 atctcccgcc gtgcacaggg tgtcacgttg caagacctgc ctgaaaccga actgcccgct
     9841 gttctgcagc cggtcgcgga ggccatggat gcgatcgctg cggccgatct tagccagacg
     9901 agcgggttcg gcccattcgg accgcaagga atcggtcaat acactacatg gcgtgatttc
     9961 atatgcgcga ttgctgatcc ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc
    10021 agtgcgtccg tcgcgcaggc tctcgatgag ctgatgcttt gggccgagga ctgccccgaa
    10081 gtccggcacc tcgtgcacgc ggatttcggc tccaacaatg tcctgacgga caatggccgc
    10141 ataacagcgg tcattgactg gagcgaggcg atgttcgggg attcccaata cgaggtcgcc
    10201 aacatcttct tctggaggcc gtggttggct tgtatggagc agcagacgcg ctacttcgag
    10261 cggaggcatc cggagcttgc aggatcgccg cggctccggg cgtatatgct ccgcattggt
    10321 cttgaccaac tctatcagag cttggttgac ggcaatttcg atgatgcagc ttgggcgcag
    10381 ggtcgatgcg acgcaatcgt ccgatccgga gccgggactg tcgggcgtac acaaatcgcc
    10441 cgcagaagcg cggccgtctg gaccgatggc tgtgtagaag tactcgccga tagtggaaac
    10501 cgacgcccca gcactcgtcc gagggcaaag gaatagggga gatgggggag gctaactgaa
    10561 acacggaagg agacaatacc ggaaggaacc cgcgctatga cggcaataaa aagacagaat
    10621 aaaacgcacg ggtgttgggt cgtttgttca taaacgcggg gttcggtccc agggctggca
    10681 ctctgtcgat accccaccga gaccccattg gggccaatac gcccgcgttt cttccttttc
    10741 cccaccccac cccccaagtt cgggtgaagg cccagggctc gcagccaacg tcggggcggc
    10801 aggccctgcc atagccactg gccccgtggg ttagggacgg ggtcccccat ggggaatggt
    10861 ttatggttcg tgggggttat tattttgggc gttgcgtggg gtctggtcca cgactggact
    10921 gagcagacag acccatggtt tttggatggc ctgggcatgg accgcatgta ctggcgcgac
    10981 acgaacaccg ggcgtctgtg gctgccaaac acccccgacc cccaaaaacc accgcgcgga
    11041 tttctggcgt gccaagctag tcgaccaatt ctcatgtttg acagcttatc atcgcagatc
    11101 cgggcaacgt tgttgcattg ctgcaggcgc agaactggta ggtatggaag atctatacat
    11161 tgaatcaata ttggcaatta gccatattag tcattggtta tatagcataa atcaatattg
    11221 gctattggcc attgcatacg ttgtatctat atcataatat gtacatttat attggctcat
    11281 gtccaatatg accgccatgt tgacattgat tattgactag ttattaatag taatcaatta
    11341 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
    11401 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
    11461 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
    11521 ctgcccactt ggcagtacat caagtgtatc atatgccaag tccgccccct attgacgtca
    11581 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttacgg gactttccta
    11641 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
    11701 acaccaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
    11761 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaata
    11821 accccgcccc gttgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
    11881 gagctcgttt agtgaaccgt cagatctcta gaagctgggt accagct
//
