LOCUS       sequence-297148-       11965 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of FLAG-tagged TAPBPR with tapasin CT G212A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11965)
  AUTHORS   McShan AC, Devlin CA, Morozov GI, Overall SA, Moschidi D, Akella N, 
            Procko E, Sgourakis NG
  TITLE     TAPBPR promotes antigen loading on MHC-I molecules using a peptide 
            trap.
  JOURNAL   Nat Commun. 2021 May 26;12(1):3174. doi: 10.1038/s41467-021-23225-6.
  PUBMED    34039964
REFERENCE   2  (bases 1 to 11965)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-05-26"; volume: "12(1):3174. doi"; pages: " 
            10.1038/s41467-021-23225-6"
COMMENT     Sequence Label: pCEP4-FLAG-TAPBPR-tapasinCT G212A
FEATURES             Location/Qualifiers
     source          1..11965
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      7..16
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             91..114
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     polyA_signal    1598..1732
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1622..1641)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1676..1695
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(1874..1896)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      2482..4271
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     CDS             complement(4577..6502)
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     primer_bind     complement(6937..6955)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7023..7127
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7128..7196,7197..7988)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7346..7365)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8159..8747
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8648..8667
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        9364..9509
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             9549..10574
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             9549..10574
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     primer_bind     complement(10595..10614)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    10639..10687
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        11340..11719
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        11720..11923
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     11874..11894
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     11920..11944
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 gctagcgcca ccatggccgt catggcgccc cgaaccctcg tcctgctact ctcgggggcc
       61 ctggccctga cccagacctg ggcgggctcc gactacaagg acgatgatga caagggagga
      121 tccaagcccc acccagcaga ggggcagtgg cgggcagtgg acgtggtcct agactgtttc
      181 ctggtgaagg acggtgcgca ccgtggagct ctcgccagca gtgaggacag ggcaagggcc
      241 tcccttgtgc tgaagcaggt gccagtgctg gacgatggct ccctggagga cttcaccgat
      301 ttccaagggg gcacactggc ccaagatgac ccacctatta tctttgaggc ctcagtggac
      361 ctggtccaga ttccccaggc cgaggccttg ctccatgctg actgcagtgg gaaggaggtg
      421 acctgtgaga tctcccgcta ctttctccag atgacagaga ccactgttaa gacagcagct
      481 tggttcatgg ccaacgtgca ggtctctgga gggggaccta gcatctcctt ggtgatgaag
      541 actcccaggg tcgccaagaa tgaggtgctc tggcacccaa cgctgaactt gccactgagc
      601 ccccagggga ctgtgcgaac tgcagtggag ttccaggtga tgacacagac ccaatccctg
      661 agcttcctgc tggggtcctc agcctccttg gactgtggct tctccatggc accgggcttg
      721 gacctcatca gtgtggagtg gcgactgcag cacaaggcca ggggtcagtt ggtgtacagc
      781 tggaccgcag ggcaggggca ggctgtgcgg aagggcgcta ccctggagcc tgcacaactg
      841 ggcatggcca gggatgcctc cctcaccctg cccggcctca ctatacagga cgaggggacc
      901 tacatttgcc agatcaccac ctctctgtac cgagctcagc agatcatcca gctcaacatc
      961 caagcttccc ctaaagtacg actgagcttg gcaaacgaag ctctgctgcc caccctcatc
     1021 tgcgacattg ctggctatta ccctctggat gtggtggtga cgtggacccg agaggagctg
     1081 ggtggatccc cagcccaagt ctctggtgcc tccttctcca gcctcaggca aagcgtggca
     1141 ggcacctaca gcatctcctc ctctctcacc gcagaacctg gctctgcagg tgccacttac
     1201 acctgccagg tcacacacat ctctctggag gagccccttg gggccagcac ccaggttgtc
     1261 ccaccagagc ggagatcagg gccctccctt gaggacagcg taggcctttt cctgtctgcc
     1321 tttcttctgc ttgggctctt caaggcactg ggctgggctg ctgtctacct gtccacctgc
     1381 aaggattcaa agaaggtaca gtgctccacc tctctgtatc tttcccttgt cactttatct
     1441 cctcatccta tctcaaaacc catggaggga ggctgctggt gtggtaggca gaacctaggc
     1501 ttggaattca cactgatctg ggttaaaacc tggcactata tcctaactgt aggactcttt
     1561 gagcatgcta cttaatgact cgaggccggc aaggccggat ccagacatga taagatacat
     1621 tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat
     1681 ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa
     1741 caattgcatt cattttatgt ttcaggttca gggggaggtg gggaggtttt ttaaagcaag
     1801 taaaacctct acaaatgtgg tatggctgat tatgatccgg ctgcctcgcg cgtttcggtg
     1861 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag
     1921 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg
     1981 gcgcagccat gaccggtcga ctctagagcc ccgccgccgg acgaactaaa cctgactacg
     2041 gcatctctgc cccttcttcg ctggtacgag gagcgctttt gttttgtatt cggggcagtg
     2101 catgtaatcc cttcagttgg ttggtacaac ttgccaactg ggccctgttc cacatgtgac
     2161 acgggggggg accaaacaca aaggggttct ctgactgtag ttgacatcct tataaatgga
     2221 tgtgcacatt tgccaacact gagtggcttt catcctggag cagactttgc atgctgtgga
     2281 ctgcaacaca acattgcctt tatgtgtaac tcttggctga agctcttaca ccaatgctgg
     2341 gggacatgta cctcccaggg gcccaggaag actacgggag gctacaccaa cgtcaatcag
     2401 aggggcctgt gtagctaccg ataagcggac cctcaagagg gcattagcaa tagtgtttat
     2461 aaggccccct tgttaaccct aaacgggtag catatgcttc ccgggtagta gtatatacta
     2521 tccagactaa ccctaattca atagcatatg ttacccaacg ggaagcatat gctatcgaat
     2581 tagggttagt aaaagggtcc taaggaacag cgatatctcc caccccatga gctgtcacgg
     2641 ttttatttac atggggtcag gattccacga gggtagtgaa ccattttagt cacaagggca
     2701 gtggctgaag atcaaggagc gggcagtgaa ctctcctgaa tcttcgcctg cttcttcatt
     2761 ctccttcgtt tagctaatag aataactgct gagttgtgaa cagtaaggtg tatgtgaggt
     2821 gctcgaaaac aaggtttcag gtgacgcccc cagaataaaa tttggacggg gggttcagtg
     2881 gtggcattgt gctatgacac caatataacc ctcacaaacc ccttgggcaa taaatactag
     2941 tgtaggaatg aaacattctg aatatcttta acaatagaaa tccatggggt ggggacaagc
     3001 cgtaaagact ggatgtccat ctcacacgaa tttatggcta tgggcaacac ataatcctag
     3061 tgcaatatga tactggggtt attaagatgt gtcccaggca gggaccaaga caggtgaacc
     3121 atgttgttac actctatttg taacaagggg aaagagagtg gacgccgaca gcagcggact
     3181 ccactggttg tctctaacac ccccgaaaat taaacggggc tccacgccaa tggggcccat
     3241 aaacaaagac aagtggccac tctttttttt gaaattgtgg agtgggggca cgcgtcagcc
     3301 cccacacgcc gccctgcggt tttggactgt aaaataaggg tgtaataact tggctgattg
     3361 taaccccgct aaccactgcg gtcaaaccac ttgcccacaa aaccactaat ggcaccccgg
     3421 ggaatacctg cataagtagg tgggcgggcc aagatagggg cgcgattgct gcgatctgga
     3481 ggacaaatta cacacacttg cgcctgagcg ccaagcacag ggttgttggt cctcatattc
     3541 acgaggtcgc tgagagcacg gtgggctaat gttgccatgg gtagcatata ctacccaaat
     3601 atctggatag catatgctat cctaatctat atctgggtag cataggctat cctaatctat
     3661 atctgggtag catatgctat cctaatctat atctgggtag tatatgctat cctaatttat
     3721 atctgggtag cataggctat cctaatctat atctgggtag catatgctat cctaatctat
     3781 atctgggtag tatatgctat cctaatctgt atccgggtag catatgctat cctaatagag
     3841 attagggtag tatatgctat cctaatttat atctgggtag catatactac ccaaatatct
     3901 ggatagcata tgctatccta atctatatct gggtagcata tgctatccta atctatatct
     3961 gggtagcata ggctatccta atctatatct gggtagcata tgctatccta atctatatct
     4021 gggtagtata tgctatccta atttatatct gggtagcata ggctatccta atctatatct
     4081 gggtagcata tgctatccta atctatatct gggtagtata tgctatccta atctgtatcc
     4141 gggtagcata tgctatcctc atgcatatac agtcagcata tgatacccag tagtagagtg
     4201 ggagtgctat cctttgcata tgccgccacc tcccaagggg gcgtgaattt tcgctgcttg
     4261 tccttttcct gcatgctggt tgctcccatt cttaggtgaa tttaaggagg ccaggctaaa
     4321 gccgtcgcat gtctgattgc tcaccaggta aatgtcgcta atgttttcca acgcgagaag
     4381 gtgttgagcg cggagctgag tgacgtgaca acatgggtat gcccaattgc cccatgttgg
     4441 gaggacgaaa atggtgacaa gacagatggc cagaaataca ccaacagcac gcatgatgtc
     4501 tactggggat ttattcttta gtgcggggga atacacggct tttaatacga ttgagggcgt
     4561 ctcctaacaa gttacatcac tcctgccctt cctcaccctc atctccatca cctccttcat
     4621 ctccgtcatc tccgtcatca ccctccgcgg cagccccttc caccataggt ggaaaccagg
     4681 gaggcaaatc tactccatcg tcaaagctgc acacagtcac cctgatattg caggtaggag
     4741 cgggctttgt cataacaagg tccttaatcg catccttcaa aacctcagca aatatatgag
     4801 tttgtaaaaa gaccatgaaa taacagacaa tggactccct tagcgggcca ggttgtgggc
     4861 cgggtccagg ggccattcca aaggggagac gactcaatgg tgtaagacga cattgtggaa
     4921 tagcaagggc agttcctcgc cttaggttgt aaagggaggt cttactacct ccatatacga
     4981 acacaccggc gacccaagtt ccttcgtcgg tagtcctttc tacgtgactc ctagccagga
     5041 gagctcttaa accttctgca atgttctcaa atttcgggtt ggaacctcct tgaccacgat
     5101 gctttccaaa ccaccctcct tttttgcgcc tgcctccatc accctgaccc cggggtccag
     5161 tgcttgggcc ttctcctggg tcatctgcgg ggccctgctc tatcgctccc gggggcacgt
     5221 caggctcacc atctgggcca ccttcttggt ggtattcaaa ataatcggct tcccctacag
     5281 ggtggaaaaa tggccttcta cctggagggg gcctgcgcgg tggagacccg gatgatgatg
     5341 actgactact gggactcctg ggcctctttt ctccacgtcc acgacctctc cccctggctc
     5401 tttcacgact tccccccctg gctctttcac gtcctctacc ccggcggcct ccactacctc
     5461 ctcgaccccg gcctccacta cctcctcgac cccggcctcc actgcctcct cgaccccggc
     5521 ctccacctcc tgctcctgcc cctcctgctc ctgcccctcc tcctgctcct gcccctcctg
     5581 cccctcctgc tcctgcccct cctgcccctc ctgctcctgc ccctcctgcc cctcctgctc
     5641 ctgcccctcc tgcccctcct cctgctcctg cccctcctgc ccctcctcct gctcctgccc
     5701 ctcctgcccc tcctgctcct gcccctcctg cccctcctgc tcctgcccct cctgcccctc
     5761 ctgctcctgc ccctcctgct cctgcccctc ctgctcctgc ccctcctgct cctgcccctc
     5821 ctgcccctcc tgcccctcct cctgctcctg cccctcctgc tcctgcccct cctgcccctc
     5881 ctgcccctcc tgctcctgcc cctcctcctg ctcctgcccc tcctgcccct cctgcccctc
     5941 ctcctgctcc tgcccctcct gcccctcctc ctgctcctgc ccctcctcct gctcctgccc
     6001 ctcctgcccc tcctgcccct cctcctgctc ctgcccctcc tgcccctcct cctgctcctg
     6061 cccctcctcc tgctcctgcc cctcctgccc ctcctgcccc tcctcctgct cctgcccctc
     6121 ctcctgctcc tgcccctcct gcccctcctg cccctcctgc ccctcctcct gctcctgccc
     6181 ctcctcctgc tcctgcccct cctgctcctg cccctcccgc tcctgctcct gctcctgttc
     6241 caccgtgggt ccctttgcag ccaatgcaac ttggacgttt ttggggtctc cggacaccat
     6301 ctctatgtct tggccctgat cctgagccgc ccggggctcc tggtcttccg cctcctcgtc
     6361 ctcgtcctct tccccgtcct cgtccatggt tatcaccccc tcttctttga ggtccactgc
     6421 cgccggagcc ttctggtcca gatgtgtctc ccttctctcc taggccattt ccaggtcctg
     6481 tacctggccc ctcgtcagac atgattcaca ctaaaagaga tcaatagaca tctttattag
     6541 acgacgctca gtgaatacag ggagtgcaga ctcctgcccc ctccaacagc ccccccaccc
     6601 tcatcccctt catggtcgct gtcagacaga tccaggtctg aaaattcccc atcctccgaa
     6661 ccatcctcgt cctcatcacc aattactcgc agcccggaaa actcccgctg aacatcctca
     6721 agatttgcgt cctgagcctc aagccaggcc tcaaattcct cgtccccctt tttgctggac
     6781 ggtagggatg gggattctcg ggacccctcc tcttcctctt caaggtcacc agacagagat
     6841 gctactgggg caacggaaga aaagctgggt gcggcctgtg aggatcagct tatcgatgat
     6901 aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat
     6961 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
     7021 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     7081 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     7141 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     7201 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     7261 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     7321 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg
     7381 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     7441 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     7501 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     7561 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     7621 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg
     7681 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     7741 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     7801 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     7861 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     7921 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     7981 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     8041 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     8101 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     8161 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     8221 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     8281 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca
     8341 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     8401 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     8461 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     8521 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     8581 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     8641 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     8701 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     8761 cggccttttt acggttcctg gccttttgct gcgccgcgtg cggctgctgg agatggcgga
     8821 cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg
     8881 attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag
     8941 gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg
     9001 gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc
     9061 gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga
     9121 agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc
     9181 ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg
     9241 aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cctgcttcat ccccgtggcc
     9301 cgttgctcgc gtttgctggc ggtgtccccg gaagaaatat atttgcatgt ctttagttct
     9361 atgatgacac aaaccccgcc cagcgtcttg tcattggcga attcgaacac gcagatgcag
     9421 tcggggcggc gcggtcccag gtccacttcg catattaagg tgacgcgtgt ggcctcgaac
     9481 accgagcgac cctgcagcga cccgcttaac agcgtcaaca gcgtgccgca gatcccgggc
     9541 aatgagatat gaaaaagcct gaactcaccg cgacgtctgt cgagaagttt ctgatcgaaa
     9601 agttcgacag cgtctccgac ctgatgcagc tctcggaggg cgaagaatct cgtgctttca
     9661 gcttcgatgt aggagggcgt ggatatgtcc tgcgggtaaa tagctgcgcc gatggtttct
     9721 acaaagatcg ttatgtttat cggcactttg catcggccgc gctcccgatt ccggaagtgc
     9781 ttgacattgg ggaattcagc gagagcctga cctattgcat ctcccgccgt gcacagggtg
     9841 tcacgttgca agacctgcct gaaaccgaac tgcccgctgt tctgcagccg gtcgcggagg
     9901 ccatggatgc gatcgctgcg gccgatctta gccagacgag cgggttcggc ccattcggac
     9961 cgcaaggaat cggtcaatac actacatggc gtgatttcat atgcgcgatt gctgatcccc
    10021 atgtgtatca ctggcaaact gtgatggacg acaccgtcag tgcgtccgtc gcgcaggctc
    10081 tcgatgagct gatgctttgg gccgaggact gccccgaagt ccggcacctc gtgcacgcgg
    10141 atttcggctc caacaatgtc ctgacggaca atggccgcat aacagcggtc attgactgga
    10201 gcgaggcgat gttcggggat tcccaatacg aggtcgccaa catcttcttc tggaggccgt
    10261 ggttggcttg tatggagcag cagacgcgct acttcgagcg gaggcatccg gagcttgcag
    10321 gatcgccgcg gctccgggcg tatatgctcc gcattggtct tgaccaactc tatcagagct
    10381 tggttgacgg caatttcgat gatgcagctt gggcgcaggg tcgatgcgac gcaatcgtcc
    10441 gatccggagc cgggactgtc gggcgtacac aaatcgcccg cagaagcgcg gccgtctgga
    10501 ccgatggctg tgtagaagta ctcgccgata gtggaaaccg acgccccagc actcgtccga
    10561 gggcaaagga ataggggaga tgggggaggc taactgaaac acggaaggag acaataccgg
    10621 aaggaacccg cgctatgacg gcaataaaaa gacagaataa aacgcacggg tgttgggtcg
    10681 tttgttcata aacgcggggt tcggtcccag ggctggcact ctgtcgatac cccaccgaga
    10741 ccccattggg gccaatacgc ccgcgtttct tccttttccc caccccaccc cccaagttcg
    10801 ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag gccctgccat agccactggc
    10861 cccgtgggtt agggacgggg tcccccatgg ggaatggttt atggttcgtg ggggttatta
    10921 ttttgggcgt tgcgtggggt ctggtccacg actggactga gcagacagac ccatggtttt
    10981 tggatggcct gggcatggac cgcatgtact ggcgcgacac gaacaccggg cgtctgtggc
    11041 tgccaaacac ccccgacccc caaaaaccac cgcgcggatt tctggcgtgc caagctagtc
    11101 gaccaattct catgtttgac agcttatcat cgcagatccg ggcaacgttg ttgcattgct
    11161 gcaggcgcag aactggtagg tatggaagat ctatacattg aatcaatatt ggcaattagc
    11221 catattagtc attggttata tagcataaat caatattggc tattggccat tgcatacgtt
    11281 gtatctatat cataatatgt acatttatat tggctcatgt ccaatatgac cgccatgttg
    11341 acattgatta ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc
    11401 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
    11461 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
    11521 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
    11581 agtgtatcat atgccaagtc cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
    11641 gcattatgcc cagtacatga ccttacggga ctttcctact tggcagtaca tctacgtatt
    11701 agtcatcgct attaccatgg tgatgcggtt ttggcagtac accaatgggc gtggatagcg
    11761 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
    11821 gcaccaaaat caacgggact ttccaaaatg tcgtaataac cccgccccgt tgacgcaaat
    11881 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca
    11941 gatctctaga agctgggtac cagct
//
