LOCUS       sequence-297154-       11800 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of FLAG-tagged Tapasin L18E.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11800)
  AUTHORS   McShan AC, Devlin CA, Morozov GI, Overall SA, Moschidi D, Akella N, 
            Procko E, Sgourakis NG
  TITLE     TAPBPR promotes antigen loading on MHC-I molecules using a peptide 
            trap.
  JOURNAL   Nat Commun. 2021 May 26;12(1):3174. doi: 10.1038/s41467-021-23225-6.
  PUBMED    34039964
REFERENCE   2  (bases 1 to 11800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-05-26"; volume: "12(1):3174. doi"; pages: " 
            10.1038/s41467-021-23225-6"
COMMENT     Sequence Label: pCEP4-FLAG-Tapasin L18E
FEATURES             Location/Qualifiers
     source          1..11800
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      7..16
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             91..114
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     polyA_signal    1433..1567
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1457..1476)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1511..1530
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(1709..1731)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      2317..4106
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     CDS             complement(4412..6337)
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     primer_bind     complement(6772..6790)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6858..6962
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6963..7031,7032..7823)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7181..7200)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7994..8582
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8483..8502
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        9199..9344
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             9384..10409
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             9384..10409
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     primer_bind     complement(10430..10449)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    10474..10522
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        11175..11554
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        11555..11758
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     11709..11729
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     11755..11779
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 gctagcgcca ccatggccgt catggcgccc cgaaccctcg tcctgctact ctcgggggcc
       61 ctggccctga cccagacctg ggcgggctcc gactacaagg acgatgatga caagggagga
      121 tccggccctg ccgtaatcga gtgttggttt gttgaagacg ctagtggtaa aggggaagct
      181 aaacggccag gcgcacttct gttgcggcaa ggtccgggag aaccacctcc acgacccgac
      241 ttggaccctg aattgtacct ctctgtacac gacccagctg gagcactcca agctgctttc
      301 cgacggtacc caagaggtgc tccagcaccg cattgcgaga tgagtcggtt cgttcctttg
      361 cctgcgagtg caaagtgggc atcaggtctg acaccagcac aaaactgtcc acgagcgctt
      421 gatggagcgt ggctgatggt ttccataagc tctccggtgc tttcactgtc atcactgctc
      481 aggccacaac ctgaacccca acaagaaccc gtactgataa caatggcgac ggtagtcctt
      541 acggtcctta cgcatacacc agcacccagg gtgcgattgg gacaagacgc actcctcgac
      601 cttagctttg cgtacatgcc accaacctca gaggcggctt cctctcttgc accaggacca
      661 ccaccgttcg gccttgaatg gagacgacag cacttgggga agggccattt gctgttggct
      721 gctaccccag ggctgaacgg gcagatgccg gcagcacaag agggagccgt agcttttgct
      781 gcgtgggatg atgacgaacc atggggacca tggactggaa acgggacttt ctggctgcca
      841 acagtacagc cctttcaaga aggaacgtat ctggccacca tccatttgcc ttatctgcaa
      901 ggccaggtca cacttgaact cgctgtctat aaaccgccta aggtgagtct catgcccgca
      961 actttggcta gggcagcacc tggcgaagct ccacctgaat tgctctgttt ggtatcccat
     1021 ttctacccca gtggtggcct tgaagtggaa tgggaactta gaggaggacc aggtggtcga
     1081 tctcagaaag ctgaagggca gcgatggctc tccgctctca ggcaccactc agacggatct
     1141 gtatcactgt cagggcattt gcagccaccg ccagttacca ccgagcagca tggagctcgg
     1201 tatgcgtgtc gcattcacca ccctagcctt ccggcgtcag gaagatcagc ggaggtaaca
     1261 cttgaggtcg ctgggttgtc cggacccagc ttggaggaca gtgttggtct gttcctgtcc
     1321 gctttcctgt tgctgggtct gtttaaggca ctcggatggg cagcagttta tctcagcacc
     1381 tgtaaagaca gcaagaaaaa ggcagaatag taactcgagg ccggcaaggc cggatccaga
     1441 catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg
     1501 ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa
     1561 acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtggggag
     1621 gttttttaaa gcaagtaaaa cctctacaaa tgtggtatgg ctgattatga tccggctgcc
     1681 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
     1741 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
     1801 ttggcgggtg tcggggcgca gccatgaccg gtcgactcta gagccccgcc gccggacgaa
     1861 ctaaacctga ctacggcatc tctgcccctt cttcgctggt acgaggagcg cttttgtttt
     1921 gtattcgggg cagtgcatgt aatcccttca gttggttggt acaacttgcc aactgggccc
     1981 tgttccacat gtgacacggg gggggaccaa acacaaaggg gttctctgac tgtagttgac
     2041 atccttataa atggatgtgc acatttgcca acactgagtg gctttcatcc tggagcagac
     2101 tttgcatgct gtggactgca acacaacatt gcctttatgt gtaactcttg gctgaagctc
     2161 ttacaccaat gctgggggac atgtacctcc caggggccca ggaagactac gggaggctac
     2221 accaacgtca atcagagggg cctgtgtagc taccgataag cggaccctca agagggcatt
     2281 agcaatagtg tttataaggc ccccttgtta accctaaacg ggtagcatat gcttcccggg
     2341 tagtagtata tactatccag actaacccta attcaatagc atatgttacc caacgggaag
     2401 catatgctat cgaattaggg ttagtaaaag ggtcctaagg aacagcgata tctcccaccc
     2461 catgagctgt cacggtttta tttacatggg gtcaggattc cacgagggta gtgaaccatt
     2521 ttagtcacaa gggcagtggc tgaagatcaa ggagcgggca gtgaactctc ctgaatcttc
     2581 gcctgcttct tcattctcct tcgtttagct aatagaataa ctgctgagtt gtgaacagta
     2641 aggtgtatgt gaggtgctcg aaaacaaggt ttcaggtgac gcccccagaa taaaatttgg
     2701 acggggggtt cagtggtggc attgtgctat gacaccaata taaccctcac aaaccccttg
     2761 ggcaataaat actagtgtag gaatgaaaca ttctgaatat ctttaacaat agaaatccat
     2821 ggggtgggga caagccgtaa agactggatg tccatctcac acgaatttat ggctatgggc
     2881 aacacataat cctagtgcaa tatgatactg gggttattaa gatgtgtccc aggcagggac
     2941 caagacaggt gaaccatgtt gttacactct atttgtaaca aggggaaaga gagtggacgc
     3001 cgacagcagc ggactccact ggttgtctct aacacccccg aaaattaaac ggggctccac
     3061 gccaatgggg cccataaaca aagacaagtg gccactcttt tttttgaaat tgtggagtgg
     3121 gggcacgcgt cagcccccac acgccgccct gcggttttgg actgtaaaat aagggtgtaa
     3181 taacttggct gattgtaacc ccgctaacca ctgcggtcaa accacttgcc cacaaaacca
     3241 ctaatggcac cccggggaat acctgcataa gtaggtgggc gggccaagat aggggcgcga
     3301 ttgctgcgat ctggaggaca aattacacac acttgcgcct gagcgccaag cacagggttg
     3361 ttggtcctca tattcacgag gtcgctgaga gcacggtggg ctaatgttgc catgggtagc
     3421 atatactacc caaatatctg gatagcatat gctatcctaa tctatatctg ggtagcatag
     3481 gctatcctaa tctatatctg ggtagcatat gctatcctaa tctatatctg ggtagtatat
     3541 gctatcctaa tttatatctg ggtagcatag gctatcctaa tctatatctg ggtagcatat
     3601 gctatcctaa tctatatctg ggtagtatat gctatcctaa tctgtatccg ggtagcatat
     3661 gctatcctaa tagagattag ggtagtatat gctatcctaa tttatatctg ggtagcatat
     3721 actacccaaa tatctggata gcatatgcta tcctaatcta tatctgggta gcatatgcta
     3781 tcctaatcta tatctgggta gcataggcta tcctaatcta tatctgggta gcatatgcta
     3841 tcctaatcta tatctgggta gtatatgcta tcctaattta tatctgggta gcataggcta
     3901 tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta gtatatgcta
     3961 tcctaatctg tatccgggta gcatatgcta tcctcatgca tatacagtca gcatatgata
     4021 cccagtagta gagtgggagt gctatccttt gcatatgccg ccacctccca agggggcgtg
     4081 aattttcgct gcttgtcctt ttcctgcatg ctggttgctc ccattcttag gtgaatttaa
     4141 ggaggccagg ctaaagccgt cgcatgtctg attgctcacc aggtaaatgt cgctaatgtt
     4201 ttccaacgcg agaaggtgtt gagcgcggag ctgagtgacg tgacaacatg ggtatgccca
     4261 attgccccat gttgggagga cgaaaatggt gacaagacag atggccagaa atacaccaac
     4321 agcacgcatg atgtctactg gggatttatt ctttagtgcg ggggaataca cggcttttaa
     4381 tacgattgag ggcgtctcct aacaagttac atcactcctg cccttcctca ccctcatctc
     4441 catcacctcc ttcatctccg tcatctccgt catcaccctc cgcggcagcc ccttccacca
     4501 taggtggaaa ccagggaggc aaatctactc catcgtcaaa gctgcacaca gtcaccctga
     4561 tattgcaggt aggagcgggc tttgtcataa caaggtcctt aatcgcatcc ttcaaaacct
     4621 cagcaaatat atgagtttgt aaaaagacca tgaaataaca gacaatggac tcccttagcg
     4681 ggccaggttg tgggccgggt ccaggggcca ttccaaaggg gagacgactc aatggtgtaa
     4741 gacgacattg tggaatagca agggcagttc ctcgccttag gttgtaaagg gaggtcttac
     4801 tacctccata tacgaacaca ccggcgaccc aagttccttc gtcggtagtc ctttctacgt
     4861 gactcctagc caggagagct cttaaacctt ctgcaatgtt ctcaaatttc gggttggaac
     4921 ctccttgacc acgatgcttt ccaaaccacc ctcctttttt gcgcctgcct ccatcaccct
     4981 gaccccgggg tccagtgctt gggccttctc ctgggtcatc tgcggggccc tgctctatcg
     5041 ctcccggggg cacgtcaggc tcaccatctg ggccaccttc ttggtggtat tcaaaataat
     5101 cggcttcccc tacagggtgg aaaaatggcc ttctacctgg agggggcctg cgcggtggag
     5161 acccggatga tgatgactga ctactgggac tcctgggcct cttttctcca cgtccacgac
     5221 ctctccccct ggctctttca cgacttcccc ccctggctct ttcacgtcct ctaccccggc
     5281 ggcctccact acctcctcga ccccggcctc cactacctcc tcgaccccgg cctccactgc
     5341 ctcctcgacc ccggcctcca cctcctgctc ctgcccctcc tgctcctgcc cctcctcctg
     5401 ctcctgcccc tcctgcccct cctgctcctg cccctcctgc ccctcctgct cctgcccctc
     5461 ctgcccctcc tgctcctgcc cctcctgccc ctcctcctgc tcctgcccct cctgcccctc
     5521 ctcctgctcc tgcccctcct gcccctcctg ctcctgcccc tcctgcccct cctgctcctg
     5581 cccctcctgc ccctcctgct cctgcccctc ctgctcctgc ccctcctgct cctgcccctc
     5641 ctgctcctgc ccctcctgcc cctcctgccc ctcctcctgc tcctgcccct cctgctcctg
     5701 cccctcctgc ccctcctgcc cctcctgctc ctgcccctcc tcctgctcct gcccctcctg
     5761 cccctcctgc ccctcctcct gctcctgccc ctcctgcccc tcctcctgct cctgcccctc
     5821 ctcctgctcc tgcccctcct gcccctcctg cccctcctcc tgctcctgcc cctcctgccc
     5881 ctcctcctgc tcctgcccct cctcctgctc ctgcccctcc tgcccctcct gcccctcctc
     5941 ctgctcctgc ccctcctcct gctcctgccc ctcctgcccc tcctgcccct cctgcccctc
     6001 ctcctgctcc tgcccctcct cctgctcctg cccctcctgc tcctgcccct cccgctcctg
     6061 ctcctgctcc tgttccaccg tgggtccctt tgcagccaat gcaacttgga cgtttttggg
     6121 gtctccggac accatctcta tgtcttggcc ctgatcctga gccgcccggg gctcctggtc
     6181 ttccgcctcc tcgtcctcgt cctcttcccc gtcctcgtcc atggttatca ccccctcttc
     6241 tttgaggtcc actgccgccg gagccttctg gtccagatgt gtctcccttc tctcctaggc
     6301 catttccagg tcctgtacct ggcccctcgt cagacatgat tcacactaaa agagatcaat
     6361 agacatcttt attagacgac gctcagtgaa tacagggagt gcagactcct gccccctcca
     6421 acagcccccc caccctcatc cccttcatgg tcgctgtcag acagatccag gtctgaaaat
     6481 tccccatcct ccgaaccatc ctcgtcctca tcaccaatta ctcgcagccc ggaaaactcc
     6541 cgctgaacat cctcaagatt tgcgtcctga gcctcaagcc aggcctcaaa ttcctcgtcc
     6601 ccctttttgc tggacggtag ggatggggat tctcgggacc cctcctcttc ctcttcaagg
     6661 tcaccagaca gagatgctac tggggcaacg gaagaaaagc tgggtgcggc ctgtgaggat
     6721 cagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga
     6781 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca
     6841 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     6901 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     6961 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     7021 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     7081 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     7141 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     7201 cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     7261 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     7321 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     7381 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     7441 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     7501 tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     7561 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     7621 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     7681 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     7741 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     7801 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     7861 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     7921 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     7981 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     8041 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     8101 aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt
     8161 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     8221 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     8281 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     8341 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     8401 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     8461 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     8521 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     8581 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctgcgcc gcgtgcggct
     8641 gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt
     8701 tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg
     8761 ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg
     8821 cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag
     8881 gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc
     8941 gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc
     9001 tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc
     9061 cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccctgc
     9121 ttcatccccg tggcccgttg ctcgcgtttg ctggcggtgt ccccggaaga aatatatttg
     9181 catgtcttta gttctatgat gacacaaacc ccgcccagcg tcttgtcatt ggcgaattcg
     9241 aacacgcaga tgcagtcggg gcggcgcggt cccaggtcca cttcgcatat taaggtgacg
     9301 cgtgtggcct cgaacaccga gcgaccctgc agcgacccgc ttaacagcgt caacagcgtg
     9361 ccgcagatcc cgggcaatga gatatgaaaa agcctgaact caccgcgacg tctgtcgaga
     9421 agtttctgat cgaaaagttc gacagcgtct ccgacctgat gcagctctcg gagggcgaag
     9481 aatctcgtgc tttcagcttc gatgtaggag ggcgtggata tgtcctgcgg gtaaatagct
     9541 gcgccgatgg tttctacaaa gatcgttatg tttatcggca ctttgcatcg gccgcgctcc
     9601 cgattccgga agtgcttgac attggggaat tcagcgagag cctgacctat tgcatctccc
     9661 gccgtgcaca gggtgtcacg ttgcaagacc tgcctgaaac cgaactgccc gctgttctgc
     9721 agccggtcgc ggaggccatg gatgcgatcg ctgcggccga tcttagccag acgagcgggt
     9781 tcggcccatt cggaccgcaa ggaatcggtc aatacactac atggcgtgat ttcatatgcg
     9841 cgattgctga tccccatgtg tatcactggc aaactgtgat ggacgacacc gtcagtgcgt
     9901 ccgtcgcgca ggctctcgat gagctgatgc tttgggccga ggactgcccc gaagtccggc
     9961 acctcgtgca cgcggatttc ggctccaaca atgtcctgac ggacaatggc cgcataacag
    10021 cggtcattga ctggagcgag gcgatgttcg gggattccca atacgaggtc gccaacatct
    10081 tcttctggag gccgtggttg gcttgtatgg agcagcagac gcgctacttc gagcggaggc
    10141 atccggagct tgcaggatcg ccgcggctcc gggcgtatat gctccgcatt ggtcttgacc
    10201 aactctatca gagcttggtt gacggcaatt tcgatgatgc agcttgggcg cagggtcgat
    10261 gcgacgcaat cgtccgatcc ggagccggga ctgtcgggcg tacacaaatc gcccgcagaa
    10321 gcgcggccgt ctggaccgat ggctgtgtag aagtactcgc cgatagtgga aaccgacgcc
    10381 ccagcactcg tccgagggca aaggaatagg ggagatgggg gaggctaact gaaacacgga
    10441 aggagacaat accggaagga acccgcgcta tgacggcaat aaaaagacag aataaaacgc
    10501 acgggtgttg ggtcgtttgt tcataaacgc ggggttcggt cccagggctg gcactctgtc
    10561 gataccccac cgagacccca ttggggccaa tacgcccgcg tttcttcctt ttccccaccc
    10621 caccccccaa gttcgggtga aggcccaggg ctcgcagcca acgtcggggc ggcaggccct
    10681 gccatagcca ctggccccgt gggttaggga cggggtcccc catggggaat ggtttatggt
    10741 tcgtgggggt tattattttg ggcgttgcgt ggggtctggt ccacgactgg actgagcaga
    10801 cagacccatg gtttttggat ggcctgggca tggaccgcat gtactggcgc gacacgaaca
    10861 ccgggcgtct gtggctgcca aacacccccg acccccaaaa accaccgcgc ggatttctgg
    10921 cgtgccaagc tagtcgacca attctcatgt ttgacagctt atcatcgcag atccgggcaa
    10981 cgttgttgca ttgctgcagg cgcagaactg gtaggtatgg aagatctata cattgaatca
    11041 atattggcaa ttagccatat tagtcattgg ttatatagca taaatcaata ttggctattg
    11101 gccattgcat acgttgtatc tatatcataa tatgtacatt tatattggct catgtccaat
    11161 atgaccgcca tgttgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc
    11221 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc
    11281 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
    11341 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca
    11401 cttggcagta catcaagtgt atcatatgcc aagtccgccc cctattgacg tcaatgacgg
    11461 taaatggccc gcctggcatt atgcccagta catgacctta cgggactttc ctacttggca
    11521 gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc agtacaccaa
    11581 tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa
    11641 tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta ataaccccgc
    11701 cccgttgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg
    11761 tttagtgaac cgtcagatct ctagaagctg ggtaccagct
//
