LOCUS       sequence-297159-       11815 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of FLAG-tagged Tapasin with TAPBPR a.a. 22-35.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11815)
  AUTHORS   McShan AC, Devlin CA, Morozov GI, Overall SA, Moschidi D, Akella N, 
            Procko E, Sgourakis NG
  TITLE     TAPBPR promotes antigen loading on MHC-I molecules using a peptide 
            trap.
  JOURNAL   Nat Commun. 2021 May 26;12(1):3174. doi: 10.1038/s41467-021-23225-6.
  PUBMED    34039964
REFERENCE   2  (bases 1 to 11815)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-05-26"; volume: "12(1):3174. doi"; pages: " 
            10.1038/s41467-021-23225-6"
COMMENT     Sequence Label: pCEP4-FLAG-Tapasin-TAPBPR 22-35
FEATURES             Location/Qualifiers
     source          1..11815
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      7..16
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             91..114
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     polyA_signal    1448..1582
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1472..1491)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1526..1545
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(1724..1746)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      2332..4121
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     CDS             complement(4427..6352)
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     primer_bind     complement(6787..6805)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6873..6977
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6978..7046,7047..7838)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7196..7215)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8009..8597
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8498..8517
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        9214..9359
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             9399..10424
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             9399..10424
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     primer_bind     complement(10445..10464)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    10489..10537
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        11190..11569
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        11570..11773
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     11724..11744
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     11770..11794
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 gctagcgcca ccatggccgt catggcgccc cgaaccctcg tcctgctact ctcgggggcc
       61 ctggccctga cccagacctg ggcgggctcc gactacaagg acgatgatga caagggagga
      121 tccggccctg ccgtaatcga gtgttggttt gttaaggacg gtgcgcaccg tggagctctc
      181 gccagcagtg aggacaaacg gccaggcgca cttctgttgc ggcaaggtcc gggagaacca
      241 cctccacgac ccgacttgga ccctgaattg tacctctctg tacacgaccc agctggagca
      301 ctccaagctg ctttccgacg gtacccaaga ggtgctccag caccgcattg cgagatgagt
      361 cggttcgttc ctttgcctgc gagtgcaaag tgggcatcag gtctgacacc agcacaaaac
      421 tgtccacgag cgcttgatgg agcgtggctg atggtttcca taagctctcc ggtgctttca
      481 ctgtcatcac tgctcaggcc acaacctgaa ccccaacaag aacccgtact gataacaatg
      541 gcgacggtag tccttacggt ccttacgcat acaccagcac ccagggtgcg attgggacaa
      601 gacgcactcc tcgaccttag ctttgcgtac atgccaccaa cctcagaggc ggcttcctct
      661 cttgcaccag gaccaccacc gttcggcctt gaatggagac gacagcactt ggggaagggc
      721 catttgctgt tggctgctac cccagggctg aacgggcaga tgccggcagc acaagaggga
      781 gccgtagctt ttgctgcgtg ggatgatgac gaaccatggg gaccatggac tggaaacggg
      841 actttctggc tgccaacagt acagcccttt caagaaggaa cgtatctggc caccatccat
      901 ttgccttatc tgcaaggcca ggtcacactt gaactcgctg tctataaacc gcctaaggtg
      961 agtctcatgc ccgcaacttt ggctagggca gcacctggcg aagctccacc tgaattgctc
     1021 tgtttggtat cccatttcta ccccagtggt ggccttgaag tggaatggga acttagagga
     1081 ggaccaggtg gtcgatctca gaaagctgaa gggcagcgat ggctctccgc tctcaggcac
     1141 cactcagacg gatctgtatc actgtcaggg catttgcagc caccgccagt taccaccgag
     1201 cagcatggag ctcggtatgc gtgtcgcatt caccacccta gccttccggc gtcaggaaga
     1261 tcagcggagg taacacttga ggtcgctggg ttgtccggac ccagcttgga ggacagtgtt
     1321 ggtctgttcc tgtccgcttt cctgttgctg ggtctgttta aggcactcgg atgggcagca
     1381 gtttatctca gcacctgtaa agacagcaag aaaaaggcag aatagtaact cgaggccggc
     1441 aaggccggat ccagacatga taagatacat tgatgagttt ggacaaacca caactagaat
     1501 gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat
     1561 tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca
     1621 gggggaggtg gggaggtttt ttaaagcaag taaaacctct acaaatgtgg tatggctgat
     1681 tatgatccgg ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc
     1741 tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg
     1801 gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat gaccggtcga ctctagagcc
     1861 ccgccgccgg acgaactaaa cctgactacg gcatctctgc cccttcttcg ctggtacgag
     1921 gagcgctttt gttttgtatt cggggcagtg catgtaatcc cttcagttgg ttggtacaac
     1981 ttgccaactg ggccctgttc cacatgtgac acgggggggg accaaacaca aaggggttct
     2041 ctgactgtag ttgacatcct tataaatgga tgtgcacatt tgccaacact gagtggcttt
     2101 catcctggag cagactttgc atgctgtgga ctgcaacaca acattgcctt tatgtgtaac
     2161 tcttggctga agctcttaca ccaatgctgg gggacatgta cctcccaggg gcccaggaag
     2221 actacgggag gctacaccaa cgtcaatcag aggggcctgt gtagctaccg ataagcggac
     2281 cctcaagagg gcattagcaa tagtgtttat aaggccccct tgttaaccct aaacgggtag
     2341 catatgcttc ccgggtagta gtatatacta tccagactaa ccctaattca atagcatatg
     2401 ttacccaacg ggaagcatat gctatcgaat tagggttagt aaaagggtcc taaggaacag
     2461 cgatatctcc caccccatga gctgtcacgg ttttatttac atggggtcag gattccacga
     2521 gggtagtgaa ccattttagt cacaagggca gtggctgaag atcaaggagc gggcagtgaa
     2581 ctctcctgaa tcttcgcctg cttcttcatt ctccttcgtt tagctaatag aataactgct
     2641 gagttgtgaa cagtaaggtg tatgtgaggt gctcgaaaac aaggtttcag gtgacgcccc
     2701 cagaataaaa tttggacggg gggttcagtg gtggcattgt gctatgacac caatataacc
     2761 ctcacaaacc ccttgggcaa taaatactag tgtaggaatg aaacattctg aatatcttta
     2821 acaatagaaa tccatggggt ggggacaagc cgtaaagact ggatgtccat ctcacacgaa
     2881 tttatggcta tgggcaacac ataatcctag tgcaatatga tactggggtt attaagatgt
     2941 gtcccaggca gggaccaaga caggtgaacc atgttgttac actctatttg taacaagggg
     3001 aaagagagtg gacgccgaca gcagcggact ccactggttg tctctaacac ccccgaaaat
     3061 taaacggggc tccacgccaa tggggcccat aaacaaagac aagtggccac tctttttttt
     3121 gaaattgtgg agtgggggca cgcgtcagcc cccacacgcc gccctgcggt tttggactgt
     3181 aaaataaggg tgtaataact tggctgattg taaccccgct aaccactgcg gtcaaaccac
     3241 ttgcccacaa aaccactaat ggcaccccgg ggaatacctg cataagtagg tgggcgggcc
     3301 aagatagggg cgcgattgct gcgatctgga ggacaaatta cacacacttg cgcctgagcg
     3361 ccaagcacag ggttgttggt cctcatattc acgaggtcgc tgagagcacg gtgggctaat
     3421 gttgccatgg gtagcatata ctacccaaat atctggatag catatgctat cctaatctat
     3481 atctgggtag cataggctat cctaatctat atctgggtag catatgctat cctaatctat
     3541 atctgggtag tatatgctat cctaatttat atctgggtag cataggctat cctaatctat
     3601 atctgggtag catatgctat cctaatctat atctgggtag tatatgctat cctaatctgt
     3661 atccgggtag catatgctat cctaatagag attagggtag tatatgctat cctaatttat
     3721 atctgggtag catatactac ccaaatatct ggatagcata tgctatccta atctatatct
     3781 gggtagcata tgctatccta atctatatct gggtagcata ggctatccta atctatatct
     3841 gggtagcata tgctatccta atctatatct gggtagtata tgctatccta atttatatct
     3901 gggtagcata ggctatccta atctatatct gggtagcata tgctatccta atctatatct
     3961 gggtagtata tgctatccta atctgtatcc gggtagcata tgctatcctc atgcatatac
     4021 agtcagcata tgatacccag tagtagagtg ggagtgctat cctttgcata tgccgccacc
     4081 tcccaagggg gcgtgaattt tcgctgcttg tccttttcct gcatgctggt tgctcccatt
     4141 cttaggtgaa tttaaggagg ccaggctaaa gccgtcgcat gtctgattgc tcaccaggta
     4201 aatgtcgcta atgttttcca acgcgagaag gtgttgagcg cggagctgag tgacgtgaca
     4261 acatgggtat gcccaattgc cccatgttgg gaggacgaaa atggtgacaa gacagatggc
     4321 cagaaataca ccaacagcac gcatgatgtc tactggggat ttattcttta gtgcggggga
     4381 atacacggct tttaatacga ttgagggcgt ctcctaacaa gttacatcac tcctgccctt
     4441 cctcaccctc atctccatca cctccttcat ctccgtcatc tccgtcatca ccctccgcgg
     4501 cagccccttc caccataggt ggaaaccagg gaggcaaatc tactccatcg tcaaagctgc
     4561 acacagtcac cctgatattg caggtaggag cgggctttgt cataacaagg tccttaatcg
     4621 catccttcaa aacctcagca aatatatgag tttgtaaaaa gaccatgaaa taacagacaa
     4681 tggactccct tagcgggcca ggttgtgggc cgggtccagg ggccattcca aaggggagac
     4741 gactcaatgg tgtaagacga cattgtggaa tagcaagggc agttcctcgc cttaggttgt
     4801 aaagggaggt cttactacct ccatatacga acacaccggc gacccaagtt ccttcgtcgg
     4861 tagtcctttc tacgtgactc ctagccagga gagctcttaa accttctgca atgttctcaa
     4921 atttcgggtt ggaacctcct tgaccacgat gctttccaaa ccaccctcct tttttgcgcc
     4981 tgcctccatc accctgaccc cggggtccag tgcttgggcc ttctcctggg tcatctgcgg
     5041 ggccctgctc tatcgctccc gggggcacgt caggctcacc atctgggcca ccttcttggt
     5101 ggtattcaaa ataatcggct tcccctacag ggtggaaaaa tggccttcta cctggagggg
     5161 gcctgcgcgg tggagacccg gatgatgatg actgactact gggactcctg ggcctctttt
     5221 ctccacgtcc acgacctctc cccctggctc tttcacgact tccccccctg gctctttcac
     5281 gtcctctacc ccggcggcct ccactacctc ctcgaccccg gcctccacta cctcctcgac
     5341 cccggcctcc actgcctcct cgaccccggc ctccacctcc tgctcctgcc cctcctgctc
     5401 ctgcccctcc tcctgctcct gcccctcctg cccctcctgc tcctgcccct cctgcccctc
     5461 ctgctcctgc ccctcctgcc cctcctgctc ctgcccctcc tgcccctcct cctgctcctg
     5521 cccctcctgc ccctcctcct gctcctgccc ctcctgcccc tcctgctcct gcccctcctg
     5581 cccctcctgc tcctgcccct cctgcccctc ctgctcctgc ccctcctgct cctgcccctc
     5641 ctgctcctgc ccctcctgct cctgcccctc ctgcccctcc tgcccctcct cctgctcctg
     5701 cccctcctgc tcctgcccct cctgcccctc ctgcccctcc tgctcctgcc cctcctcctg
     5761 ctcctgcccc tcctgcccct cctgcccctc ctcctgctcc tgcccctcct gcccctcctc
     5821 ctgctcctgc ccctcctcct gctcctgccc ctcctgcccc tcctgcccct cctcctgctc
     5881 ctgcccctcc tgcccctcct cctgctcctg cccctcctcc tgctcctgcc cctcctgccc
     5941 ctcctgcccc tcctcctgct cctgcccctc ctcctgctcc tgcccctcct gcccctcctg
     6001 cccctcctgc ccctcctcct gctcctgccc ctcctcctgc tcctgcccct cctgctcctg
     6061 cccctcccgc tcctgctcct gctcctgttc caccgtgggt ccctttgcag ccaatgcaac
     6121 ttggacgttt ttggggtctc cggacaccat ctctatgtct tggccctgat cctgagccgc
     6181 ccggggctcc tggtcttccg cctcctcgtc ctcgtcctct tccccgtcct cgtccatggt
     6241 tatcaccccc tcttctttga ggtccactgc cgccggagcc ttctggtcca gatgtgtctc
     6301 ccttctctcc taggccattt ccaggtcctg tacctggccc ctcgtcagac atgattcaca
     6361 ctaaaagaga tcaatagaca tctttattag acgacgctca gtgaatacag ggagtgcaga
     6421 ctcctgcccc ctccaacagc ccccccaccc tcatcccctt catggtcgct gtcagacaga
     6481 tccaggtctg aaaattcccc atcctccgaa ccatcctcgt cctcatcacc aattactcgc
     6541 agcccggaaa actcccgctg aacatcctca agatttgcgt cctgagcctc aagccaggcc
     6601 tcaaattcct cgtccccctt tttgctggac ggtagggatg gggattctcg ggacccctcc
     6661 tcttcctctt caaggtcacc agacagagat gctactgggg caacggaaga aaagctgggt
     6721 gcggcctgtg aggatcagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac
     6781 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
     6841 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     6901 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     6961 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     7021 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     7081 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     7141 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     7201 gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact
     7261 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     7321 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     7381 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     7441 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     7501 agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     7561 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     7621 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     7681 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     7741 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     7801 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     7861 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     7921 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     7981 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     8041 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     8101 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc
     8161 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     8221 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     8281 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     8341 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     8401 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     8461 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     8521 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     8581 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     8641 gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt
     8701 ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc
     8761 gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga
     8821 cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc
     8881 catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt
     8941 aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc
     9001 ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata
     9061 atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg
     9121 gccgccatgc cctgcttcat ccccgtggcc cgttgctcgc gtttgctggc ggtgtccccg
     9181 gaagaaatat atttgcatgt ctttagttct atgatgacac aaaccccgcc cagcgtcttg
     9241 tcattggcga attcgaacac gcagatgcag tcggggcggc gcggtcccag gtccacttcg
     9301 catattaagg tgacgcgtgt ggcctcgaac accgagcgac cctgcagcga cccgcttaac
     9361 agcgtcaaca gcgtgccgca gatcccgggc aatgagatat gaaaaagcct gaactcaccg
     9421 cgacgtctgt cgagaagttt ctgatcgaaa agttcgacag cgtctccgac ctgatgcagc
     9481 tctcggaggg cgaagaatct cgtgctttca gcttcgatgt aggagggcgt ggatatgtcc
     9541 tgcgggtaaa tagctgcgcc gatggtttct acaaagatcg ttatgtttat cggcactttg
     9601 catcggccgc gctcccgatt ccggaagtgc ttgacattgg ggaattcagc gagagcctga
     9661 cctattgcat ctcccgccgt gcacagggtg tcacgttgca agacctgcct gaaaccgaac
     9721 tgcccgctgt tctgcagccg gtcgcggagg ccatggatgc gatcgctgcg gccgatctta
     9781 gccagacgag cgggttcggc ccattcggac cgcaaggaat cggtcaatac actacatggc
     9841 gtgatttcat atgcgcgatt gctgatcccc atgtgtatca ctggcaaact gtgatggacg
     9901 acaccgtcag tgcgtccgtc gcgcaggctc tcgatgagct gatgctttgg gccgaggact
     9961 gccccgaagt ccggcacctc gtgcacgcgg atttcggctc caacaatgtc ctgacggaca
    10021 atggccgcat aacagcggtc attgactgga gcgaggcgat gttcggggat tcccaatacg
    10081 aggtcgccaa catcttcttc tggaggccgt ggttggcttg tatggagcag cagacgcgct
    10141 acttcgagcg gaggcatccg gagcttgcag gatcgccgcg gctccgggcg tatatgctcc
    10201 gcattggtct tgaccaactc tatcagagct tggttgacgg caatttcgat gatgcagctt
    10261 gggcgcaggg tcgatgcgac gcaatcgtcc gatccggagc cgggactgtc gggcgtacac
    10321 aaatcgcccg cagaagcgcg gccgtctgga ccgatggctg tgtagaagta ctcgccgata
    10381 gtggaaaccg acgccccagc actcgtccga gggcaaagga ataggggaga tgggggaggc
    10441 taactgaaac acggaaggag acaataccgg aaggaacccg cgctatgacg gcaataaaaa
    10501 gacagaataa aacgcacggg tgttgggtcg tttgttcata aacgcggggt tcggtcccag
    10561 ggctggcact ctgtcgatac cccaccgaga ccccattggg gccaatacgc ccgcgtttct
    10621 tccttttccc caccccaccc cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc
    10681 ggggcggcag gccctgccat agccactggc cccgtgggtt agggacgggg tcccccatgg
    10741 ggaatggttt atggttcgtg ggggttatta ttttgggcgt tgcgtggggt ctggtccacg
    10801 actggactga gcagacagac ccatggtttt tggatggcct gggcatggac cgcatgtact
    10861 ggcgcgacac gaacaccggg cgtctgtggc tgccaaacac ccccgacccc caaaaaccac
    10921 cgcgcggatt tctggcgtgc caagctagtc gaccaattct catgtttgac agcttatcat
    10981 cgcagatccg ggcaacgttg ttgcattgct gcaggcgcag aactggtagg tatggaagat
    11041 ctatacattg aatcaatatt ggcaattagc catattagtc attggttata tagcataaat
    11101 caatattggc tattggccat tgcatacgtt gtatctatat cataatatgt acatttatat
    11161 tggctcatgt ccaatatgac cgccatgttg acattgatta ttgactagtt attaatagta
    11221 atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac
    11281 ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac
    11341 gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt
    11401 acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagtc cgccccctat
    11461 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttacggga
    11521 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt
    11581 ttggcagtac accaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca
    11641 ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg
    11701 tcgtaataac cccgccccgt tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta
    11761 tataagcaga gctcgtttag tgaaccgtca gatctctaga agctgggtac cagct
//
