LOCUS       sequence-297199-        7481 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression plasmid for SARS-CoV-2 surface glycoprotein 
            (Spike protein).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 7481)
  TITLE     Truncated SARS-CoV-2 Spike protein constructs for making VSV pseudo 
            virus
REFERENCE   2  (bases 1 to 7481)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGBW-m4137386
FEATURES             Location/Qualifiers
     source          1..7481
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     complement(4499..4518)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    4564..4619
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4618..4637)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4980..4996)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4980..4996)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4993..5015)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5004..5020
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5028..5034,5035..5052,5053..5058))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5073..5094
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        5153..5348
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      5199..5334
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    5354..5488
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5391..5410)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5445..5464
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5556..5573)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5727..6315)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5807..5826)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             join(6486..6554,6555..7346)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6704..6723)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7347..7451)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc
      601 agatcagatc tttgtcgatc ctaccatcca ctcgacacac ccgccagcgg ccgccaccat
      661 gtttgttttt cttgttttat tgccactagt ctctagtcag tgtgttaatc ttacaaccag
      721 aactcaatta ccccctgcat acactaattc tttcacacgt ggtgtttatt accctgacaa
      781 agttttcaga tcctcagttt tacattcaac tcaggacttg ttcttacctt tcttttccaa
      841 tgttacttgg ttccatgcta tacatgtctc tgggaccaat ggtactaaga ggtttgataa
      901 ccctgtccta ccatttaatg atggtgttta ttttgcttcc actgagaagt ctaacataat
      961 aagaggctgg atttttggta ctactttaga ttcgaagacc cagtccctac ttattgttaa
     1021 taacgctact aatgttgtta ttaaagtctg tgaatttcaa ttttgtaatg atccattttt
     1081 gggtgtttat taccacaaaa acaacaaaag ttggatggaa agtgagttca gagtttattc
     1141 tagtgcgaat aattgcactt ttgaatatgt ctctcagcct tttcttatgg accttgaagg
     1201 aaaacagggt aatttcaaaa atcttaggga atttgtgttt aagaatattg atggttattt
     1261 taaaatatat tctaagcaca cgcctattaa tttagtgcgt gatctccctc agggtttttc
     1321 ggctttagaa ccattggtag atttgccaat aggtattaac atcactaggt ttcaaacttt
     1381 acttgcttta catagaagtt atttgactcc tggtgattct tcttcaggtt ggacagctgg
     1441 tgctgcagct tattatgtgg gttatcttca acctaggact tttctattaa aatataatga
     1501 aaatggaacc attacagatg ctgtagactg tgcacttgac cctctctcag aaacaaagtg
     1561 tacgttgaaa tccttcactg tagaaaaagg aatctatcaa acttctaact ttagagtcca
     1621 accaacagaa tctattgtta gatttcctaa tattacaaac ttgtgccctt ttggtgaagt
     1681 ttttaacgcc accagatttg catctgttta tgcttggaac aggaagagaa tcagcaactg
     1741 tgttgctgat tattctgtcc tatataattc cgcatcattt tccactttta agtgttatgg
     1801 agtgtctcct actaaattaa atgatctctg ctttactaat gtctatgcag attcatttgt
     1861 aattagaggt gatgaagtca gacaaatcgc tccagggcaa actggaaaga ttgctgatta
     1921 taattataaa ttaccagatg attttacagg ctgcgttata gcttggaatt ctaacaatct
     1981 tgattctaag gttggtggta attataatta cctgtataga ttgtttagga agtctaatct
     2041 caaacctttt gagagagata tttcaactga aatctatcag gccggtagca caccttgtaa
     2101 tggtgttgaa ggttttaatt gttactttcc tttacaatca tatggtttcc aacccactaa
     2161 tggtgttggt taccaaccat acagagtagt agtactttct tttgaacttc tacatgcacc
     2221 agcaactgtt tgtggaccta aaaagtctac taatttggtt aaaaacaaat gtgtcaattt
     2281 caacttcaat ggtttaacag gcacaggtgt tcttactgag tctaacaaaa agtttctgcc
     2341 tttccaacaa tttggcagag acattgctga cactactgat gctgtccgtg atccacagac
     2401 acttgagatt cttgacatta caccatgttc ttttggtggt gtcagtgtta taacaccagg
     2461 aacaaatact tctaaccagg ttgctgttct ttatcaggat gttaactgca cagaagtccc
     2521 tgttgctatt catgcagatc aacttactcc tacttggcgt gtttattcta caggttctaa
     2581 tgtttttcaa acacgtgcag gctgtttaat aggggctgaa catgtcaaca actcatatga
     2641 gtgtgacata cccattggtg caggtatatg cgctagttat cagactcaga ctaattctcc
     2701 tcggcgggca cgtagtgtag ctagtcaatc catcattgcc tacactatgt cacttggtgc
     2761 agaaaattca gttgcttact ctaataactc tattgccata cccacaaatt ttactattag
     2821 tgttaccaca gaaattctac cagtgtctat gaccaagaca tcagtagatt gtacaatgta
     2881 catttgtggt gattcaactg aatgcagcaa tcttttgttg caatatggca gtttttgtac
     2941 acaattaaac cgtgctttaa ctggaatagc tgttgaacaa gacaaaaaca cccaagaagt
     3001 ttttgcacaa gtcaaacaaa tttacaaaac accaccaatt aaagattttg gtggttttaa
     3061 tttttcacaa atattaccag atccatcaaa accaagcaag aggtcattta ttgaagatct
     3121 acttttcaac aaagtgacac ttgcagatgc tggcttcatc aaacaatatg gtgattgcct
     3181 tggtgatatt gctgctagag acctcatttg tgcacaaaag tttaacggcc ttactgtttt
     3241 gccacctttg ctcacagatg aaatgattgc tcaatacact tctgcactgt tagcgggtac
     3301 aatcacttct ggttggacct ttggtgcagg tgctgcatta caaataccat ttgctatgca
     3361 aatggcttat aggtttaatg gtattggagt tacacagaat gttctctatg agaaccaaaa
     3421 attgattgcc aaccaattta atagtgctat tggcaaaatt caagactcac tttcttccac
     3481 agcaagtgca cttggaaaac ttcaagatgt ggtcaaccaa aatgcacaag ctttaaacac
     3541 gcttgttaaa caacttagct ccaattttgg tgcaatttca agtgttttaa atgatatcct
     3601 ttcacgtctt gacaaagttg aggctgaagt gcaaattgat aggttgatca caggcagact
     3661 tcaaagtttg cagacatatg tgactcaaca attaattaga gctgcagaaa tcagagcttc
     3721 tgctaatctt gctgctacta aaatgtcaga gtgtgtactt ggacaatcaa aaagagttga
     3781 tttttgtgga aagggctatc atcttatgtc cttccctcag tcagcacctc atggtgtagt
     3841 cttcttgcat gtgacttatg tccctgcaca agaaaagaac ttcacaactg ctcctgccat
     3901 ttgtcatgat ggaaaagcac actttcctcg tgaaggtgtc tttgtttcaa atggcacaca
     3961 ctggtttgta acacaaagga atttttatga accacaaatc attactacag acaacacatt
     4021 tgtgtctggt aactgtgatg ttgtaatagg aattgtcaac aacacagttt atgatccttt
     4081 gcaacctgaa ttagactcat tcaaggagga gttagataaa tattttaaga atcatacatc
     4141 accagatgtt gatttaggtg acatctctgg cattaatgct tcagttgtaa acattcaaaa
     4201 agaaattgac cgcctcaatg aggttgccaa gaatttaaat gaatctctca tcgatctcca
     4261 agaacttgga aagtatgagc agtatataaa atggccatgg tacatttggc taggttttat
     4321 agctggcttg attgccatag taatggtgac aattatgctt tgctgtatga ccagttgctg
     4381 tagttgtctc aagggctgtt gttcttgtgg atcctgctgc aaatttgatg aagacgactc
     4441 tgagccagtg ctcaaaggag tcaaattaca ttacacataa tccggactca gatctcgatc
     4501 tttttccctc tgccaaaaat tatggggaca tcatgaagcc ccttgagcat ctgacttctg
     4561 gctaataaag gaaatttatt ttcattgcaa tagtgtgttg gaattttttg tgtctctcac
     4621 tcggaaggac atatgggagg gcaaatcatt taaaacatca gaatgagtat ttggtttaga
     4681 gtttggcaac atatgcccat atgctggctg ccatgaacaa aggttggcta taaagaggtc
     4741 atcagtatat gaaacagccc cctgctgtcc attccttatt ccatagaaaa gccttgactt
     4801 gaggttagat tttttttata ttttgttttg tgttattttt ttctttaaca tccctaaaat
     4861 tttccttaca tgttttacta gccagatttt tcctcctctc ctgactactc ccagtcatag
     4921 ctgtccctct tctcttatgg agatccctcg acctgcagcc caagcttggc gtaatcatgg
     4981 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     5041 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     5101 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagcggat ccgcatctca
     5161 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     5221 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     5281 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     5341 tttgcaaaaa gctaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     5401 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     5461 catcaatgta tcttatcatg tctggatccg ctgcattaat gaatcggcca acgcgcgggg
     5521 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     5581 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     5641 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     5701 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     5761 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     5821 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     5881 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     5941 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     6001 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     6061 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     6121 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     6181 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     6241 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     6301 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     6361 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     6421 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     6481 gacagatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     6541 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     6601 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     6661 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     6721 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     6781 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     6841 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     6901 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     6961 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     7021 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     7081 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     7141 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     7201 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     7261 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     7321 gtgcctcact gattaagcat tggtaaactc ttcctttttc aatattattg aagcatttat
     7381 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     7441 ggggttccgc gcacatttcc ccgaaaagtg ccacctgggt c
//
