LOCUS       sequence-297200-        2178 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  New color variant of Fucci (Fluorescent ubiquitination-based cell 
            cycle indicator) : Fucci(CA)5..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2178)
  AUTHORS   Ando R, Sakaue-Sawano A, Shoda K, Miyawaki A
  TITLE     Two coral fluorescent proteins of distinct colors for sharp 
            visualization of cell-cycle progression.
  JOURNAL   Cell Struct Funct. 2023 Jun 30. doi: 10.1247/csf.23028.
  PUBMED    37394513
REFERENCE   2  (bases 1 to 2178)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Struct
            Funct. 2023 Jun 30. doi: 10.1247/csf.23028."
COMMENT     Sequence Label: tFucci(CA)5
FEATURES             Location/Qualifiers
     source          1..2178
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1048..1104
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             1846..2175
                     /codon_start=1
                     /product="degron consisting of residues 1-110 of human 
                     Geminin (Zielke and Edgar, 2015)"
                     /label=Gem1 (1-110)
                     /translation="MNPSMKQKQEEIKENIKNSSVPRRTLKMIQPSASGSLVGRENELS
                     AGLSKRKHRNDHLTSTTSSPGVIVPESSENKNLGGVTQESFDLMIKENPSSQYWKEVAE
                     KRRKAL"
ORIGIN
        1 atggagaacg tcaggagaaa gacaggcatt cagacggaaa tgaaaacgaa gctccacatg
       61 gatggtatgg tgaatggaca cagctttgag atcaaagggg aggggaaggg aagcccgtac
      121 gagggggtgc agacaatgaa gctgaaggtt actaagggcg ccccacttcc cttctctata
      181 gacattcttt tgcctcagtg catgtatggc agcaaacctt tcattaaata tcctgagaac
      241 atccccgatt atttgaaact ctcatttcca gagggtatca cttgggagag gacaatgaca
      301 ttcgaggacg gcgcagtatg cgatgtatct aatgattcca gactggtcgg caattgcttt
      361 atctacactg tgaagtttca gggagtcaat tttccacttg atggccccgt tatgcaaaag
      421 aagacccgag gctgggaacc ctccactgaa gtgctgtacg aatgtgatgg gtggatgcgc
      481 gggttggtgg acatagctct gaaactggaa aacggtggac attatatgtg taattttaag
      541 accacctaca aatccaagaa aggtcttgag gtgccacctt accatttcgt ggatcataaa
      601 ctcgacctgc tcagtcataa caccgacgga gccacctttg aggagttcga acaaggtgaa
      661 atcgctcacg cgcacttgag taaactggcc ggatatccat cacactggcg gccgctcgag
      721 atggagcagc gccgcgtcac cgacttcttc gcgcgccgcc gccccgggcc cccccgcatc
      781 gcgccgccca agctggcctg ccgcaccccc agccccgcca ggcccgcact ccgcgccccg
      841 gcctccgcta ccagtggcag ccgcaagcgc gcccgcccgc ccgccgcccc cggacgcgac
      901 caggccaggc caccggcccg cgcggccgcg cggctgtcgg tggacgaggt ttccagcccc
      961 agtacccccg aggccccaga catcccagcc tgcccttctc cgggccagaa gataaagaaa
     1021 accggtcgcg ccaagcgcgg cagcggcgcc accaacttca gcctgctgaa gcaggccggc
     1081 gacgtggagg agaaccccgg cccccgcgaa ttcatgagta aactccagaa aggtgtggag
     1141 aaagagatga agattaagct cactatgaac tgcaccgtga acgaacggaa ctttgtgatc
     1201 acagggcagg gggctgggga accttatgat ggaactcaga cactttacct gacagtggag
     1261 ggcggaaaga cactgaactt cagcttcgat gtactgaccc cagcctttca gtacggcaat
     1321 agggcgttta ccaagtaccc cggtaacata ccagacttct tcaaacagac cttcagtggc
     1381 gggggataca catggaaaag aacaatgacc tatgaagacg gcggcgtctc aaccgttgag
     1441 tccgatatca gcgttcaagg ggactgcttc cattataaaa tacaattcaa cggaaagttc
     1501 cccccccacg gcccagtcat gcaaaaggaa acagtcaaat gggaaccttc caccgaggtg
     1561 atgtacaagg acgataaaaa cgatggagtg ctgaagggcg acgtgaatat ggccctgctg
     1621 ttgaaggatg gtggtcacct cagagtcgat ttcaacactt cttatattcc caaaaataag
     1681 gtggagaaga tgccagatta ccacttcgtg gaccaccgga tcgagatcct ggagaagccc
     1741 gaaggacgcc ctgtcaagct ctacgcaggg gctgtggcca ggtacagcct gctgcctgaa
     1801 aagaacctga ataagggata tccatcacac tggcggccgc tcgagatgaa tcccagtatg
     1861 aagcagaaac aagaagaaat caaagagaat ataaagaata gttctgtccc aagaagaact
     1921 ctgaagatga ttcagccttc tgcatctgga tctcttgttg gaagagaaaa tgagctgtcc
     1981 gcaggcttgt ccaaaaggaa acatcggaat gaccacttaa catctacaac ttccagccct
     2041 ggggttattg tcccagaatc tagtgaaaat aaaaatcttg gaggagtcac ccaggagtca
     2101 tttgatctta tgattaaaga aaatccatcc tctcagtatt ggaaggaagt ggcagaaaaa
     2161 cggagaaagg cgctgtaa
//
