LOCUS       sequence-297210-        9343 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9343)
  AUTHORS   Yen HC, Xu Q, Chou DM, Zhao Z, Elledge SJ
  TITLE     Global protein stability profiling in mammalian cells.
  JOURNAL   Science. 2008 Nov 7;322(5903):918-23. doi: 10.1126/science.1160489.
  PUBMED    18988847
REFERENCE   2  (bases 1 to 9343)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2008-11-7- 7"; volume: "322"; issue: 
            "5903"; pages: "918-23"
COMMENT     Sequence Label: MSCV-CMV-DsRed-IRES-EGFP-DEST
FEATURES             Location/Qualifiers
     source          1..9343
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..517
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     misc_feature    581..922
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             989..1405
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1335..1357
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1373..1389
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     protein_bind    1436..1560
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(1601..1906)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(1703..1722)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             complement(2248..2907)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2822..2841
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(join(2961..2967,2968..2985,2986..2991))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(3016..3140)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(join(3168..3878,3879..3881,3882..3884))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(3168..3878,3879..3881,3882..3884))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3210..3231)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     3559..3578
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3818..3839
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     misc_feature    join(3885..3893,3894..3896,3897..4471)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4058..4077)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     4287..4304
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     CDS             complement(4514..5191)
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERAEGRHH
                     LFL"
     primer_bind     complement(4576..4599)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(4774..4793)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     4991..5011
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     5043..5061
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      5188..5197
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(5343..5367)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(5364..5567)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(5393..5413)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        5568..5871
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     protein_bind    6414..6430
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6438..6444,6445..6462,6463..6468))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6483..6504
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6621..6638)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6792..7380)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6872..6891)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7551..8342,8343..8411))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8174..8193
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8412..8516)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8584..8602
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8640..8662)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8762..8781
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8975..8997
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9125..9144
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 aatgaaagac cccacctgta ggtttggcaa gctagcttaa gtaacgccat tttgcaaggc
       61 atggaaaata cataactgag aatagagaag ttcagatcaa ggttaggaac agagagacag
      121 cagaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa
      181 gaacagatgg tccccagatg cggtcccgcc ctcagcagtt tctagagaac catcagatgt
      241 ttccagggtg ccccaaggac ctgaaatgac cctgtgcctt atttgaacta accaatcagt
      301 tcgcttctcg cttctgttcg cgcgcttctg ctccccgagc tcaataaaag agcccacaac
      361 ccctcactcg gcgcgccagt cctccgatag actgcgtcgc ccgggtaccc gtattcccaa
      421 taaagcctct tgctgtttgc atccgaatcg tggactcgct gatccttggg agggtctcct
      481 cagattgatt gactgcccac ctcgggggtc tttcatttgg aggttccacc gagatttgga
      541 gacccctgcc cagggaccac cgaccccccc gccgggaggt aagctggcca gcggtcgttt
      601 cgtgtctgtc tctgtctttg tgcgtgtttg tgccggcatc taatgtttgc gcctgcgtct
      661 gtactagtta gctaactagc tctgtatctg gcggacccgt ggtggaactg acgagttctg
      721 aacacccggc cgcaaccctg ggagacgtcc cagggacttt gggggccgtt tttgtggccc
      781 gacctgagga agggagtcga tgtggaatcc gaccccgtca ggatatgtgg ttctggtagg
      841 agacgagaac ctaaaacagt tcccgcctcc gtctgaattt ttgctttcgg tttggaaccg
      901 aagccgcgcg tcttgtctgc tgcagcgctg cagcatcgtt ctgtgttgtc tctgtctgac
      961 tgtgtttctg tatttgtctg aaaattaggg ccagactgtt accactccct taagtttgac
     1021 cttaggtcac tggaaagatg tcgagcggat cgctcacaac cagtcggtag atgtcaagaa
     1081 gagacgttgg gttaccttct gctctgcaga atggccaacc tttaacgtcg gatggccgcg
     1141 agacggcacc tttaaccgag acctcatcac ccaggttaag atcaaggtct tttcacctgg
     1201 cccgcatgga cacccagacc aggtccccta catcgtgacc tgggaagcct tggcttttga
     1261 cccccctccc tgggtcaagc cctttgtaca ccctaagcct ccgcctcctc ttcctccatc
     1321 cgccccgtct ctcccccttg aacctcctcg ttcgaccccg cctcgatcct ccctttatcc
     1381 agccctcact ccttctctag gcgccggaat tagatctcag ttatctaggc cgatcaccac
     1441 tttgtacaag aaagctgaac gagaaacgta aaatgatata aatatcaata tattaaatta
     1501 gattttgcat aaaaaacaga ctacataata ctgtaaaaca caacatatcc agtcactatg
     1561 gtcgacctgc agactggctg tgtataaggg agcctgacat ttatattccc cagaacatca
     1621 ggttaatggc gtttttgatg tcattttcgc ggtggctgag atcagccact tcttccccga
     1681 taacggagac cggcacactg gccatatcgg tggtcatcat gcgccagctt tcatccccga
     1741 tatgcaccac cgggtaaagt tcacgggaga ctttatctga cagcagacgt gcactggcca
     1801 gggggatcac catccgtcgc ccgggcgtgt caataatatc actctgtaca tccacaaaca
     1861 gacgataacg gctctctctt ttataggtgt aaaccttaaa ctgcatttca ccagcccctg
     1921 ttctcgtcag caaaagagcc gttcatttca ataaaccggg cgacctcagc catcccttcc
     1981 tgattttccg ctttccagcg ttcggcacgc agacgacggg cttcattctg catggttgtg
     2041 cttaccagac cggagatatt gacatcatat atgccttgag caactgatag ctgtcgctgt
     2101 caactgtcac tgtaatacgc tgcttcatag catacctctt tttgacatac ttcgggtata
     2161 catatcagta tatattctta taccgcaaaa atcagcgcgc aaatacgcat actgttatct
     2221 ggcttttagt aagccggatc cacgcgttta cgccccgccc tgccactcat cgcagtactg
     2281 ttgtaattca ttaagcattc tgccgacatg gaagccatca caaacggcat gatgaacctg
     2341 aatcgccagc ggcatcagca ccttgtcgcc ttgcgtataa tatttgccca tggtgaaaac
     2401 gggggcgaag aagttgtcca tattggccac gtttaaatca aaactggtga aactcaccca
     2461 gggattggct gagacgaaaa acatattctc aataaaccct ttagggaaat aggccaggtt
     2521 ttcaccgtaa cacgccacat cttgcgaata tatgtgtaga aactgccgga aatcgtcgtg
     2581 gtattcactc cagagcgatg aaaacgtttc agtttgctca tggaaaacgg tgtaacaagg
     2641 gtgaacacta tcccatatca ccagctcacc gtctttcatt gccatacgga attccggatg
     2701 agcattcatc aggcgggcaa gaatgtgaat aaaggccgga taaaacttgt gcttattttt
     2761 ctttacggtc tttaaaaagg ccgtaatatc cagctgaacg gtctggttat aggtacattg
     2821 agcaactgac tgaaatgcct caaaatgttc tttacgatgc cattgggata tatcaacggt
     2881 ggtatatcca gtgatttttt tctccatttt agcttcctta gctcctgaaa atctcgacgg
     2941 atcctaactc aaaatccaca cattatacga gccggaagca taaagtgtaa agcctggggt
     3001 gcctaatgcg gccgccatag tgactggata tgttgtgttt tacagtatta tgtagtctgt
     3061 tttttatgca aaatctaatt taatatattg atatttatat cattttacgt ttctcgttca
     3121 gcttttttgt acaaacttgt gattggccag atctgagtcc ggccggactt gtacagctcg
     3181 tccatgccga gagtgatccc ggcggcggtc acgaactcca gcaggaccat gtgatcgcgc
     3241 ttctcgttgg ggtctttgct cagggcggac tgggtgctca ggtagtggtt gtcgggcagc
     3301 agcacggggc cgtcgccgat gggggtgttc tgctggtagt ggtcggcgag ctgcacgctg
     3361 ccgtcctcga tgttgtggcg gatcttgaag ttcaccttga tgccgttctt ctgcttgtcg
     3421 gccatgatat agacgttgtg gctgttgtag ttgtactcca gcttgtgccc caggatgttg
     3481 ccgtcctcct tgaagtcgat gcccttcagc tcgatgcggt tcaccagggt gtcgccctcg
     3541 aacttcacct cggcgcgggt cttgtagttg ccgtcgtcct tgaagaagat ggtgcgctcc
     3601 tggacgtagc cttcgggcat ggcggacttg aagaagtcgt gctgcttcat gtggtcgggg
     3661 tagcggctga agcactgcac gccgtaggtc agggtggtca cgagggtggg ccagggcacg
     3721 ggcagcttgc cggtggtgca gatgaacttc agggtcagct tgccgtaggt ggcatcgccc
     3781 tcgccctcgc cggacacgct gaacttgtgg ccgtttacgt cgccgtccag ctcgaccagg
     3841 atgggcacca ccccggtgaa cagctcctcg cccttgctca ccatggttgt ggccatatta
     3901 tcatcgtgtt tttcaaagga aaaccacgtc cccgtggttc ggggggccta gacgtttttt
     3961 taacctcgac taaacacatg taaagcatgt gtaccgaggc cccagatcag atcccataca
     4021 atggggtacc ttctgggcat ccttcagccc cttgttgaat acgcttgagg agagccattt
     4081 gactctttcc acaactatcc aactcacaac gtggcactgg ggttgtgccg cctttgcagg
     4141 tgtatcttat acacgtggct tttggccgca gaggcacctg tcgccaggtg gggggttccg
     4201 ctgcctgcaa agggtcgcta cagacgttgt ttgtcttcaa gaagcttcca gaggaactgc
     4261 ttccttcacg acattcaaca gaccttgcat tcctttggcg agaggggaaa gacccctagg
     4321 aatgctcgtc aagaagacag ggccaggttt ccgggccctc acattgccaa aagacggcaa
     4381 tatggtggaa aataacatat agacaaacgc acaccggcct tattccaagc ggcttcggcc
     4441 agtaacgtta gggggggggg agggagaggg gcggatcccg ggcccgcggt accgtcgact
     4501 gcagaattgg ccgctacagg aacaggtggt ggcggccctc ggcgcgctcg tactgctcca
     4561 cgatggtgta gtcctcgttg tgggaggtga tgtccagctt ggagtccacg tagtagtagc
     4621 cgggcagctg cacgggcttc ttggccatgt agatggactt gaactccacc aggtagtggc
     4681 cgccgtcctt cagcttcagg gccttgtgga tctcgccctt cagcacgccg tcgcgggggt
     4741 acaggcgctc ggtggaggcc tcccagccca tagtcttctt ctgcattacg gggccgtcgg
     4801 aggggaagtt cacgccgatg aacttcacct tgtagatgaa ggagccgtcc tgcagggagg
     4861 agtcctgggt cacggtcacc acgccgccgt cctcgaagtt catcacgcgc tcccacttga
     4921 agccctcggg gaaggacagc ttcttgtagt cggggatgtc ggcggggtgc ttcacgtaca
     4981 ccttggagcc gtactggaac tggggggaca ggatgtccca ggcgaagggc agggggccgc
     5041 ccttggtcac cttcagcttg gcggtctggg tgccctcgta ggggcggccc tcgccctcgc
     5101 cctcgatctc gaactcgtgg ccgttcacgg agccctccat gcgcaccttg aagcgcatga
     5161 actccttgat gacgtcctcg gaggaggcca tggtggcgac cggtggatct gagtccggta
     5221 gcgctagggc cgcggatcaa ttcctgcagg tccgttccaa tgcaccgttc ccggccgcgg
     5281 aggctggatc ggtcccggtg tcttctatgg aggtcaaaac agcgtggatg gcgtctccag
     5341 gcgatctgac ggttcactaa acgagctctg cttatataga cctcccaccg tacacgccta
     5401 ccgcccattt gcgtcaatgg ggcggagttg ttacgacatt ttggaaagtc ccgttgattt
     5461 tggtgccaaa acaaactccc attgacgtca atggggtgga gacttggaaa tccccgtgag
     5521 tcaaaccgct atccacgccc attgatgtac tgccaaaacc gcatcaccat ggtaatagcg
     5581 atgactaata cgtagatgta ctgccaagta ggaaagtccc ataaggtcat gtactgggca
     5641 taatgccagg cgggccattt accgtcattg acgtcaatag ggggcgtact tggcatatga
     5701 tacacttgat gtactgccaa gtgggcagtt taccgtaaat actccaccca ttgacgtcaa
     5761 tggaaagtcc ctattggcgt tactatggga acatacgtca ttattgacgt caatgggcgg
     5821 gggtcgttgg gcggtcagcc aggcgggcca tttaccgtaa gttatgtaac ggactctaga
     5881 ggatcgataa aataaaagat tttatttagt ctccagaaaa aggggggaat gaaagacccc
     5941 acctgtaggt ttggcaagct agagaaccat cagatgtttc cagggtgccc caaggacctg
     6001 aaatgaccct gtgccttatt tgaactaacc aatcagttcg cttctcgctt ctgttcgcgc
     6061 gcttctgctc cccgagctca ataaaagagc ccacaacccc tcactcggcg cgccagtcct
     6121 ccgatagact gcgtcgcccg ggtacccgtg tatccaataa accctcttgc agttgcatcc
     6181 gacttgtggt ctcgctgttc cttgggaggg tctcctctga gtgattgact accgtcagcg
     6241 ggggtctttc atgggtaaca gtttcttgaa gttggagaac aacattctga gggtaggagt
     6301 cgaatattaa gtaatcctga ctcaattagc cactgttttg aatccacata ctccaatact
     6361 cctgaaatag ttcattatgg acagcgcaga aagagctggg gagaattgtg aaattgttat
     6421 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     6481 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     6541 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     6601 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     6661 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     6721 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     6781 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     6841 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     6901 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     6961 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     7021 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     7081 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     7141 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     7201 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     7261 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     7321 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     7381 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     7441 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     7501 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     7561 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     7621 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     7681 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     7741 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     7801 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     7861 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     7921 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     7981 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     8041 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     8101 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     8161 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     8221 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     8281 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     8341 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     8401 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     8461 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     8521 tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat
     8581 aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac
     8641 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
     8701 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat
     8761 gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga
     8821 tgcgtaagga gaaaataccg catcaggcgc cattcgccat tcaggctgcg caactgttgg
     8881 gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct
     8941 gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg
     9001 gcgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg
     9061 ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc
     9121 catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg
     9181 ccacgatgcg tccggcgtag aggcgattag tccaatttgt taaagacagg atatcagtgg
     9241 tccaggctct agttttgact caacaatatc accagctgaa gcctatagag tacgagccat
     9301 agataaaata aaagatttta tttagtctcc agaaaaaggg ggg
//
